Gene

KWMTBOMO16497  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004586

Annotation

trehalase-2_precursor_[Bombyx_mori]

Location

Bomo_Scaf003(+):81246-111635      View in EpiBrowser

Full name

Trehalase      

Alternative Name

Alpha-trehalose glucohydrolase
Alpha,alpha-trehalase
Alpha,alpha-trehalose glucohydrolase

Location in the cell

Cytoplasmic   Reliability : 1.577

CDS

ATGTTTATGGTGTGGCTTTGGGTCGGTTTTGCGATGGCCGCTAGCGTCGTTGCTGATCGGAGCAAACTACCTCCTGCCTGTAACAGTATGATTTACTGCCACGGGCCACTGTTAAACACGGTCCAAATGGCCGGCCTCTACAATGACTCGAAGACGTTTGTGGATATGAAAATTAAAATATCACCAAAGATCACGTTGGAGCACTTCTATGACATGATGAGCAGGACGGATTCTAATCCGACTAAAGCGGACATACAGGAGTTCGTGAATCAGAACTTCGATCCTGAGGGTTCGGAATTTGAAGACTGGAGGCCCAGTGATTGGAAGCATAACCCTGGATTTCTTGCTAAAATCAAGGATCCGTTGTTGCACAAATGGGCATCAGCCTTGAACGATTTATGGCTCGATCTCGGGAGGAAGATGAAGGAAGCAGTCAAAGAGAGTCCAGACTTATACTCCATTATATACGTGGAGCAACCTTTTATTGTTCCAGGTGGTCGTTTCCGTGAGTTCTACTACTGGGACTCATACTGGATCATCAAAGGTCTGCTGCTCTCGGAGATGAGGACCACAGCGAAGGGCATGGTCAACAATTTACTCAGCATAGTCGATAGATACGGCTTCATACCGAATGGCGGCAGAATTTATTATCTTATGAGATCTCAACCTCCCCTTCTGATACCGATGGTGCAGCTGCTAATGGAAGACACCGATGACCTGGAATACCTGAGGGAGCACATCCACACCCTGGACAAAGAGTTCGACTACTGGATGACGAATCACACCATCGAAATTAACCACGAAGGGAAGAAATTGAAAATGGCGATGTACATGGATAATTCTCAGGGTCCGAGGCCGGAGAGCTACAAAGAAGACGTCGACTGTGCTAGACATTTTGACACAACTGAGAAGAAAGAAGAATTGTACGCTGAATTGAAGGCAGCCGCTGAATCCGGCTGGGATTTCTCATCACGATGGTTCATACTGAACGGAACCAATAAAGGTAATCTAACTAATTTGAAGGTGAGGTCGATAATTCCCGTCGACCTCAACGCGATCTTGTGTTGGAATGCGCAGCTCATGATGGAGTACCACACCCGCCTTCAAAATGAGGAGAAAGCATCTTACTATAGGCGGATACACGATGACTTCATGGAAGCTATCGAAGAGCTACTGTGGCACGAAGACGTGGGAGTTTGGTTGGACTACAGCCTCGAGTCTAGTCGCAGGAGAGACTACTTTTACCCATCCAACCTGTCTCCGCTCTGGGCCGGCAGCTACGATAAAGCGAGAAAGGATTACTTCGTGAATAGAGTCGTCAATTATTTGGATAAAGTCAAGATGGATATCTTCGAAGGCGGAATCCCAACAACGTTCGAACACACCGGCGAACAGTGGGACTATCCCAACGCGTGGCCGCCCCTTCAGTACATCGTCATAATGGGCCTAGCCAACACCGGGCACCCGGAGGCGATGAGATACGCCACCGAACTCGCCACTAAGTGGGTGCGAAGTAACTTTGAAGTCTGGAAGCAGAAGGCCGCCATGCTGGAGAAGTACGACGCCACGATTCTGGGCGGGCTGGGTGGCGGCGGAGAATACGTCGTCCAAACCGGCTTCGGATGGAGCAATGGCGTTGTGATGTCGCTCCTAAATAGATATGGAGACGTAATCTCGGCAGCCGACAACTTCGGAACGGCGGCGGAATCGGGCGCTGTCTACGGCGCCGGGGTGGGCGCCGGTGGCGTAGTCACGGCGCTCCTAGTGGTCCTAGCTTCTTTGGCTGCTGGAACTTTAGGATTAATGGTGTACAAGAGACGTAAAGATTACGCCGGCATCTGTAACGGCGAAGTCGACAAACTCATCTCGAAAAAACCCTACACTGAACTCAAGAGTATGAACGGCGCGTCGAACCAGAAACAGCGGTGA

Protein

MFMVWLWVGFAMAASVVADRSKLPPACNSMIYCHGPLLNTVQMAGLYNDSKTFVDMKIKISPKITLEHFYDMMSRTDSNPTKADIQEFVNQNFDPEGSEFEDWRPSDWKHNPGFLAKIKDPLLHKWASALNDLWLDLGRKMKEAVKESPDLYSIIYVEQPFIVPGGRFREFYYWDSYWIIKGLLLSEMRTTAKGMVNNLLSIVDRYGFIPNGGRIYYLMRSQPPLLIPMVQLLMEDTDDLEYLREHIHTLDKEFDYWMTNHTIEINHEGKKLKMAMYMDNSQGPRPESYKEDVDCARHFDTTEKKEELYAELKAAAESGWDFSSRWFILNGTNKGNLTNLKVRSIIPVDLNAILCWNAQLMMEYHTRLQNEEKASYYRRIHDDFMEAIEELLWHEDVGVWLDYSLESSRRRDYFYPSNLSPLWAGSYDKARKDYFVNRVVNYLDKVKMDIFEGGIPTTFEHTGEQWDYPNAWPPLQYIVIMGLANTGHPEAMRYATELATKWVRSNFEVWKQKAAMLEKYDATILGGLGGGGEYVVQTGFGWSNGVVMSLLNRYGDVISAADNFGTAAESGAVYGAGVGAGGVVTALLVVLASLAAGTLGLMVYKRRKDYAGICNGEVDKLISKKPYTELKSMNGASNQKQR

Catalytic Activity

alpha,alpha-trehalose + H2O = alpha-D-glucose + beta-D-glucose

Biophysicochemical Properties

0.66 mM for alpha,alpha-trehalose (in 0.1 M sodium phosphate pH 6.75, at 35 degrees Celsius)
0.52 mM for alpha,alpha-trehalose (in 50 mM sodium phosphate pH 6.6, at 35 degrees Celsius)

Subunit

Monomer.

Similarity

Belongs to the glycosyl hydrolase 37 family.

Keywords

Complete proteome   Direct protein sequencing   Glycoprotein   Glycosidase   Hydrolase   Membrane   Reference proteome   Signal   Transmembrane   Transmembrane helix  

Feature

chain  Trehalase

Uniprot

Q3MV18   H9J4Z6   B5ATV4   A7XZC0   A0A0F6PAZ3   D2KHI9   + More
A0A2D0WLR5   A0A3G2SGP7   A0A1B2AQF4   A0A2W1BGC4   A3RLQ4   A0A1U9A8C9   A0A212ES68   A0A2R7X5I1   S4UQ18   A0A1B6KBM1   A0A1B6KL82   A0A0A9WUU0   A0A0K8TDD3   A0A146LQW6   K7R0G4   A0A1B6F1K5   A0A288VJ48   A0A1S6Q350   I3WEV3   A0A1S3D5L1   A0A1S3D5U6   C0LZJ7   A0A1S3D5M3   A0A1S3D5V1   A0A1D8I2M2   A0A2P8YXZ4   I7CKW6   A0A1B6EEF7   A0A1W4WRK7   E1ZVE9   A0A2S2PXE7   A0A1B6DUA6   V9I8L8   A0A2J7PTK0   A0A2J7PTL5   C8CGD2   A0A310SI70   A8J4S9   A0A088AUT8   A0A1W4WFC2   D6W851   E2BGR0   A0A2H8TFR3   J9JU09   A0A3L8DTI2   V5GU59   A0A1X9IF78   A0A195BF82   A0A1B6EC02   A0A0C9S2J2   E0VKM4   A0A2J7Q3Z7   G9I465   A0A0C9S020   A0A067R5E2   A0A195FE62   A0A026VXI6   E9I9Z8   A0A067QSZ4   A0A0C9REV2   F4X8H6   A0A158NBN3   K7IXR0   A0A336MHM1   A0A1W4WQA7   A0A336MLY4   U4UVG1   A0A195DS33   A0A2P8YZX5   A0A023F5Y3   T1HKP0   A0A0J7L328   A0A1Y1LKH8   A0A1Y1LN31   A0A224X931   A0A1S6Q338   A0A151WL58   A0A0L7QL65   A0A151IIY2   E0W4A8   A0A0A1X8X4   J7EJ73   A0A2A3E7C0   J9Q3L8   A0A034VU16   A0A1J1J8M2   A0A034VRC1   A0A034VSD4   A0A034VRD2   A0A034VTZ9   A0A1B0GQK5   A0A0K8W634   A0A0K8W4P2  

EC Number

3.2.1.28

Pubmed

16164606    19121390    19843188    18492231    23613237    28756777    + More
22118469    25401762    26823975    24610752    29403074    20798317    17827701    11826961    18362917    19820115    30249741    20566863    22243654    24845553    24508170    21282665    21719571    21347285    20075255    23537049    25474469    28004739    25830018    22851493    25348373   

EMBL

AB162717    BAE45249.1    BABH01025716    EU872435    ACF94698.1    EU106080    + More
ABU95354.1    KJ652558    AJK29980.1    GU211890    ADA63845.1    KU977457    ARD05075.1    MG787168    AYO46921.1    KU640202    ANY30159.1    KZ150067    PZC74122.1    EF426723    ABO20845.1    KX010600    ANC68249.1    AGBW02012867    OWR44309.1    KK857312    PTY27036.1    JX312193    AGL34007.2    GEBQ01031162    JAT08815.1    GEBQ01027776    JAT12201.1    GBHO01031362    JAG12242.1    GBRD01002317    JAG63504.1    GDHC01009483    JAQ09146.1    JX024262    AFV79627.1    GECZ01025694    JAS44075.1    KY400004    KY400005    KY400006    AQY15496.1    KX371565    AQV08187.1    JQ027051    AFL03410.1    FJ790320    ACN85421.1    KU756286    AOT99589.1    PYGN01000293    PSN49112.1    JX204291    AFO54713.1    GEDC01000986    JAS36312.1    GL434492    EFN74859.1    GGMS01000994    MBY70197.1    GEDC01008038    JAS29260.1    JR036191    JR036192    JR036193    JR036194    JR036195    JR036196    AEY56985.1    AEY56986.1    AEY56987.1    AEY56988.1    AEY56989.1    AEY56990.1    NEVH01021237    PNF19666.1    PNF19664.1    GQ397451    ACV20872.1    KQ759866    OAD62478.1    AB301556    BAF81545.1    KQ971307    EFA11183.1    GL448204    EFN85130.1    GFXV01001141    MBW12946.1    ABLF02022564    QOIP01000004    RLU23209.1    GALX01000767    JAB67699.1    KX349225    AOT82130.1    KQ976500    KYM83241.1    GEDC01001832    JAS35466.1    GBYB01015126    JAG84893.1    DS235248    EEB13930.1    NEVH01018420    PNF23300.1    JN565077    AEW67359.1    GBYB01015125    JAG84892.1    KK852732    KDR17472.1    KQ981673    KYN38309.1    KK107638    EZA48385.1    GL761864    EFZ22607.1    KK852980    KDR13013.1    GBYB01015124    JAG84891.1    GL888932    EGI57245.1    ADTU01011229    ADTU01011230    ADTU01011231    UFQS01001260    UFQT01001260    SSX10014.1    SSX29736.1    UFQS01001328    UFQT01001328    SSX10371.1    SSX30059.1    KB632384    ERL94231.1    KQ980581    KYN15329.1    PYGN01000262    PSN49799.1    GBBI01002113    JAC16599.1    ACPB03020626    LBMM01000908    KMQ97237.1    GEZM01054933    GEZM01054930    GEZM01054928    GEZM01054926    JAV73338.1    GEZM01054932    GEZM01054931    GEZM01054929    GEZM01054927    JAV73335.1    GFTR01007580    JAW08846.1    KX371563    AQV08185.1    KQ982981    KYQ48550.1    KQ414934    KOC59334.1    KQ977445    KYN02611.1    DS235886    EEB20464.1    GBXI01006976    JAD07316.1    HQ231258    ADT91708.1    KZ288356    PBC27182.1    JQ228448    GAKP01013934    GAKP01013922    GAKP01013920    AFI80736.1    JAC45018.1    GAKP01013914    JAC45038.1    CVRI01000074    CRL08140.1    GAKP01013933    GAKP01013926    JAC45019.1    GAKP01013928    GAKP01013925    GAKP01013915    JAC45037.1    GAKP01013923    GAKP01013918    GAKP01013916    JAC45029.1    GAKP01013929    JAC45023.1    AJVK01017190    AJVK01017191    GDHF01005656    JAI46658.1    GDHF01006136    JAI46178.1   

Proteomes

UP000005204    UP000007151    UP000079169    UP000245037    UP000192223    UP000000311    + More
UP000235965    UP000005203    UP000007266    UP000008237    UP000007819    UP000279307    UP000078540    UP000009046    UP000027135    UP000078541    UP000053097    UP000007755    UP000005205    UP000002358    UP000030742    UP000078492    UP000015103    UP000036403    UP000075809    UP000053825    UP000078542    UP000242457    UP000183832    UP000092462   

PRIDE

Q3MV18    H9J4Z6   

Pfam

PF01204   Trehalase

Interpro

IPR001661   Glyco_hydro_37        + More
IPR018232   Glyco_hydro_37_CS       
IPR012341   6hp_glycosidase-like_sf       
IPR008928   6-hairpin_glycosidase_sf       

SUPFAM

SSF48208   SSF48208       

Gene 3D

1.50.10.10    

ProteinModelPortal

Q3MV18   H9J4Z6   B5ATV4   A7XZC0   A0A0F6PAZ3   D2KHI9   + More
A0A2D0WLR5   A0A3G2SGP7   A0A1B2AQF4   A0A2W1BGC4   A3RLQ4   A0A1U9A8C9   A0A212ES68   A0A2R7X5I1   S4UQ18   A0A1B6KBM1   A0A1B6KL82   A0A0A9WUU0   A0A0K8TDD3   A0A146LQW6   K7R0G4   A0A1B6F1K5   A0A288VJ48   A0A1S6Q350   I3WEV3   A0A1S3D5L1   A0A1S3D5U6   C0LZJ7   A0A1S3D5M3   A0A1S3D5V1   A0A1D8I2M2   A0A2P8YXZ4   I7CKW6   A0A1B6EEF7   A0A1W4WRK7   E1ZVE9   A0A2S2PXE7   A0A1B6DUA6   V9I8L8   A0A2J7PTK0   A0A2J7PTL5   C8CGD2   A0A310SI70   A8J4S9   A0A088AUT8   A0A1W4WFC2   D6W851   E2BGR0   A0A2H8TFR3   J9JU09   A0A3L8DTI2   V5GU59   A0A1X9IF78   A0A195BF82   A0A1B6EC02   A0A0C9S2J2   E0VKM4   A0A2J7Q3Z7   G9I465   A0A0C9S020   A0A067R5E2   A0A195FE62   A0A026VXI6   E9I9Z8   A0A067QSZ4   A0A0C9REV2   F4X8H6   A0A158NBN3   K7IXR0   A0A336MHM1   A0A1W4WQA7   A0A336MLY4   U4UVG1   A0A195DS33   A0A2P8YZX5   A0A023F5Y3   T1HKP0   A0A0J7L328   A0A1Y1LKH8   A0A1Y1LN31   A0A224X931   A0A1S6Q338   A0A151WL58   A0A0L7QL65   A0A151IIY2   E0W4A8   A0A0A1X8X4   J7EJ73   A0A2A3E7C0   J9Q3L8   A0A034VU16   A0A1J1J8M2   A0A034VRC1   A0A034VSD4   A0A034VRD2   A0A034VTZ9   A0A1B0GQK5   A0A0K8W634   A0A0K8W4P2  

PDB

5Z6H     E-value=3.47667e-64,     Score=624

KEGG

692454    K01194 alpha,alpha-trehalase [EC:3.2.1.28] | (RefSeq) Treh-2; trehalase-2

PATHWAY

00500    Starch and sucrose metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0004555   GO:0016021   GO:0005991   GO:0008152   GO:0003824   GO:0008270   GO:0003676   GO:0016491   GO:0016651   GO:0046835   GO:0006281   GO:0006259  

PANTHER

PTHR23403  

Subcellular location

Membrane  

SignalP

Position:   1 - 18,         Likelihood:  0.994411

 

 

Length:

642

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

24.24536

Exp number, first 60 AAs:

0.18826

Total prob of N-in:

0.01072

outside

1  -  581

TMhelix

582  -  604

inside

605  -  642

 

 

Pi

28.577211

Theta

29.722853

Tajima's D

-0.952911

CLR

0.000121

CSRT

0.146392680365982

Interpretation

Uncertain