Gene

KWMTBOMO16370  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA013746

Annotation

prophenoloxidase_activating_enzyme_precursor_[Bombyx_mori]

Location

Bomo_Chr28(-):7434734-7451575      View in EpiBrowser

Full name

Phenoloxidase-activating enzyme      

Alternative Name

Prophenoloxidase-activating enzyme

Location in the cell

Extracellular   Reliability : 4.629

CDS

ATGTTTTTAATTTGGACATTCATCGTGGCTGTTCTGGCGATCCAGACCAAAAGTGTTGTTGCACAATCATGCCGGACACCGAACGGACTTAACGGTAATTGCGTATCCGTGTATGAATGCCAGGCACTCCTGGCCATATTAAATAATCAGAGGAGAACCCAGCAAGATGAGAAGTTCTTGAGGGACTCCCAATGCGGCACCAAGAATTCAGTTCCTGCTGTTTGCTGTCCATGCAACGCAGCTGATGGTCAGCAAGGGAATTGTGTCAACATAAATTCATGTCCGTACGTTCTTCAATTGCTGAAGAATCCGAATGAAGCGAATCTAAATTACGTCCGGGGATCTGTTTGTCAAGGATCAGAACAACAGAGCATATGCTGTGTTACTGCGCCACAGTCGACGGCAGTGACCACGACCCCCAGACCGAAGCGGGTGCACGCCTGCCAGAGCGAGATGACTGCCACCCCCCCTAATCCGGAAGGGAAGTGTTGCGGCCGGGACATAGCCGTCGGAGACAAAATCGTTGGTGGTGCGCCCGCATCCATCGACTCGTATCCTTGGCTGGTGGTCATCGAGTATGTGAGGCTCGAGAGGACGATGCTGCTCTGTGGCGGGGCGCTCATCAGCGGGAAGTACGTGCTCACTGCCGGTCATTGCGTGAAAGGAGCGATCTTGGATGTAGGCACGCCGAAAACAGTGCGTCTCGGCGAGTACAACACGACGAACCCGGGCCGTGATTGCGTGTCCGTATCAGCGGGCGGCACGGATTGCACGGACCCATTGGTCAAGATAGGCATTGAGAAGACCATACCTCACCCCGACTATCAGCCCTATCATTTTCTTAGGAAACACGACATCGGGTTGATCAGGCTGCAAAGCATCGCGCCTTTTACAGATTTCATCAGGCCTATATGCTTGCCGAGTACAGATTACACAGTGAACCCGCCAAGTAAATTCGCTCTGACCGTGGCTGGCTGGGGACGATACTTGCAGTTCGACAATGGGACAGTTAGAAGCAGCAAGATCAAGCTTCACGTAACACTGCCTTTTGTACAACGAGACGTCTGCGAAGCAAACCAGAAACCATTGCGCAATGGCCAAAGAATAACATTGTGGAAGGGACAAATGTGTGCCGGCGGTGAAGCAGGTAAAGATTCTTGTAAGGGTGACTCGGGTGGTCCTCTTATGTATGAACATTCCAAAAAATACGAAGCAGTCGGTATTGTGAGCTTCGGACCCGAAAAGTGTGGCCAGATCGATATACCTGGAGTTTACACAAATGTTTATGAATACCTGCCATGGATACAAAATACAATTGAACCTTGA

Protein

MFLIWTFIVAVLAIQTKSVVAQSCRTPNGLNGNCVSVYECQALLAILNNQRRTQQDEKFLRDSQCGTKNSVPAVCCPCNAADGQQGNCVNINSCPYVLQLLKNPNEANLNYVRGSVCQGSEQQSICCVTAPQSTAVTTTPRPKRVHACQSEMTATPPNPEGKCCGRDIAVGDKIVGGAPASIDSYPWLVVIEYVRLERTMLLCGGALISGKYVLTAGHCVKGAILDVGTPKTVRLGEYNTTNPGRDCVSVSAGGTDCTDPLVKIGIEKTIPHPDYQPYHFLRKHDIGLIRLQSIAPFTDFIRPICLPSTDYTVNPPSKFALTVAGWGRYLQFDNGTVRSSKIKLHVTLPFVQRDVCEANQKPLRNGQRITLWKGQMCAGGEAGKDSCKGDSGGPLMYEHSKKYEAVGIVSFGPEKCGQIDIPGVYTNVYEYLPWIQNTIEP

Description

Endopeptidase with selective post-Arg cleavage site (PubMed:4197814, PubMed:4197815, PubMed:10066809, PubMed:7981662). Activates prophenoloxidase (PubMed:4197814, PubMed:4197815). Has a probable role in the melanization process as part of the innate immune response (Probable).

Biophysicochemical Properties

2.2 uM for prophenoloxidase (at pH 7.6 and 0 degrees Celsius)
1.43 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the absence of KCl)
0.77 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the presence of 0.025 M KCl)
1.2 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the presence of 0.004 M KCl)
0.35 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the presence of 0.5 M KCl)
0.145 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the presence of 0.5 M KCl)
0.709 mM for N-p-tosyl-L-arginine methyl ester hydrochloride (TAME) (in the presence of 0.5 M KCl)

Subunit

In the active form, heterodimer of a light chain and a heavy chain; disulfide-linked.

Similarity

Belongs to the peptidase S1 family. CLIP subfamily.
Belongs to the peptidase S1 family.

Keywords

Calcium   Complete proteome   Direct protein sequencing   Disulfide bond   Glycoprotein   Hydrolase   Metal-binding   Protease   Pyrrolidone carboxylic acid   Reference proteome   Serine protease   Signal   Zymogen  

Feature

chain  Phenoloxidase-activating enzyme light chain

Uniprot

Q9XXV0   Q2FAY5   Q8I917   A0A2A4JWX6   A0A290U627   Q49QW0   + More
A1IIA5   Q6Y2X4   Q5DI98   Q5MPC0   Q2FAY7   Q5MPC2   Q2FAY6   I4DJE3   A0A126U9N5   A0A140EQ25   A0A0N1INY1   S4PHS5   Q2FAY4   A0A0N1IMQ8   A0A212EUU4   I4DS78   A0A0U1WT40   A0A173GPF0   A0A212F8R3   A0A0L7L0F2   H9JW83   V5GX21   D2XPX4   K7J980   A0A2A3EA55   A0A1Y1L107   A0A154P5I7   A0A212F8P6   A0A173GPF7   A0A195FEH3   U4U606   A0A0J7NWE6   A0A1I8N0W4   J3JYT9   A0A088A359   A0A158NI84   E2C8L2   A0A151WR74   A0A0C9QZC0   A0A1I8PP27   A0A195EG37   F4WPR9   A0A026WIN7   B1B5K1   A0JCK6   A0A1A9VW86   A0A3L8D7J0   A0A1W4XI92   A0A0N1PH79   B4N8B1   B3NYQ7   A0A034WRL1   N6TWW7   A0A0K8WKM9   W8CBZ2   A0A0K8U524   A0A0A1WGL4   A0A034VLF9   A0A0L7QXF0   A0A195C079   A0A0A1XFE0   A0A0M9A7E2   E2AV85   A0A1Y1KZL1   A0A3S2M731   A0A0L0CE89   Q17B76   A0A1A9X740   A0A1B0FQW4   A0A1I8JTB4   T1DTQ9   A0A0K8VB28   A0A182XUW4   A0A1Y1K9U3   A0A182VAF6   Q9Y1K6   A0A034V075   Q7QKL2   A0A0A1X7H3   A0A1Y1L1S6   A0A195AWL1   A0A1W4VMD7   W8BLL3   B3F5B1   A0A0A1XAL3   A0A1A9Z167   A0A182IIR9   A0A023EQV6   A0A182G580   A0A1Y1K3W7   A0A336LMT0   Q7QB73  

EC Number

3.4.21.-

Pubmed

10066809    19121390    4197814    4197815    7981662    16324822    + More
12456683    28953952    17307003    14505699    16033436    15944088    22651552    26354079    23622113    22118469    26227816    20075255    28004739    23537049    25315136    22516182    21347285    20798317    21719571    24508170    18195005    30249741    17994087    25348373    24495485    25830018    26108605    17510324    10646969    12364791    14747013    17210077    24945155    26483478   

EMBL

AB009670    BABH01025554    BAA76308.1    DQ115323    AAZ91696.1    AY077643    + More
AAL76085.1    NWSH01000467    PCG76208.1    KY680241    ATD13322.1    AY677082    AAW24481.1    AB264091    BAF43530.1    AY188445    AAO74570.1    AH014670    AY789466    AAX18637.1    AY672790    AAV91012.1    AAZ91694.1    AY672788    AAV91010.1    AAZ91695.1    AK401411    BAM18033.1    KT751522    AML39475.1    KT751521    AML39474.1    KQ460936    KPJ10700.1    GAIX01000374    JAA92186.1    DQ115324    AAZ91697.1    KQ459547    KPJ00023.1    AGBW02012316    OWR45231.1    AK405456    BAM20768.1    JQ581598    AFX82594.1    KT006138    ANH58163.1    AGBW02009696    OWR50099.1    JTDY01003981    KOB68749.1    BABH01025543    BABH01025544    GALX01002154    JAB66312.1    GU229936    ADA84072.3    AAZX01002213    KZ288322    PBC28172.1    GEZM01073843    JAV64787.1    KQ434809    KZC06390.1    OWR50098.1    KT006137    ANH58162.1    KQ981636    KYN38803.1    KB631684    ERL85365.1    LBMM01001207    KMQ96720.1    BT128420    AEE63377.1    ADTU01016095    GL453666    EFN75742.1    KQ982813    KYQ50307.1    GBYB01001065    JAG70832.1    KQ978957    KYN27230.1    GL888255    EGI63810.1    KK107185    EZA55838.1    AB363980    BAG14262.2    AB282644    BAF36824.1    QOIP01000012    RLU16063.1    KQ459999    KPJ18497.1    CH964232    EDW81362.1    CH954181    EDV48170.1    GAKP01002127    JAC56825.1    APGK01057809    KB741282    ENN70807.1    GDHF01000894    JAI51420.1    GAMC01000094    JAC06462.1    GDHF01030580    JAI21734.1    GBXI01016739    GBXI01006351    JAC97552.1    JAD07941.1    GAKP01015995    JAC42957.1    KQ414704    KOC63285.1    KQ978501    KYM93571.1    GBXI01004615    JAD09677.1    KQ435719    KOX78755.1    GL443059    EFN62639.1    GEZM01073844    JAV64786.1    RSAL01000017    RVE52975.1    JRES01000503    KNC30561.1    CH477325    EAT43491.1    CCAG010023843    APCN01002147    GAMD01000809    JAB00782.1    GDHF01016160    JAI36154.1    GEZM01092820    JAV56445.1    AF117749    AAD38335.1    GAKP01023416    JAC35542.1    AAAB01008794    EAA03566.2    GBXI01007200    JAD07092.1    GEZM01066955    JAV67623.1    KQ976725    KYM76561.1    GAMC01016016    JAB90539.1    EF614332    ABU98654.1    GBXI01006130    JAD08162.1    APCN01002556    GAPW01002093    JAC11505.1    JXUM01042955    JXUM01042956    KQ561344    KXJ78893.1    GEZM01097973    JAV54026.1    UFQT01000091    SSX19534.1    AAAB01008880    EAA08507.4   

Proteomes

UP000005204    UP000218220    UP000053240    UP000053268    UP000007151    UP000037510    + More
UP000002358    UP000242457    UP000076502    UP000078541    UP000030742    UP000036403    UP000095301    UP000005203    UP000005205    UP000008237    UP000075809    UP000095300    UP000078492    UP000007755    UP000053097    UP000078200    UP000279307    UP000192223    UP000007798    UP000008711    UP000019118    UP000053825    UP000078542    UP000053105    UP000000311    UP000283053    UP000037069    UP000008820    UP000092443    UP000092444    UP000075840    UP000076407    UP000075903    UP000007062    UP000078540    UP000192221    UP000092445    UP000069940    UP000249989   

PRIDE

Q9XXV0    H9JW83   

Pfam

PF12032   CLIP        + More
PF00089   Trypsin
PF13019   Sde2_N_Ubi

Interpro

IPR022700   CLIP        + More
IPR033116   TRYPSIN_SER       
IPR038565   CLIP_sf       
IPR009003   Peptidase_S1_PA       
IPR001314   Peptidase_S1A       
IPR018114   TRYPSIN_HIS       
IPR001254   Trypsin_dom       
IPR029071   Ubiquitin-like_domsf       
IPR024974   Sde2_N       

SUPFAM

SSF50494   SSF50494        + More
SSF54236   SSF54236       

Gene 3D

2.20.20.90    

CDD

cd00190  Tryp_SPc

ProteinModelPortal

Q9XXV0   Q2FAY5   Q8I917   A0A2A4JWX6   A0A290U627   Q49QW0   + More
A1IIA5   Q6Y2X4   Q5DI98   Q5MPC0   Q2FAY7   Q5MPC2   Q2FAY6   I4DJE3   A0A126U9N5   A0A140EQ25   A0A0N1INY1   S4PHS5   Q2FAY4   A0A0N1IMQ8   A0A212EUU4   I4DS78   A0A0U1WT40   A0A173GPF0   A0A212F8R3   A0A0L7L0F2   H9JW83   V5GX21   D2XPX4   K7J980   A0A2A3EA55   A0A1Y1L107   A0A154P5I7   A0A212F8P6   A0A173GPF7   A0A195FEH3   U4U606   A0A0J7NWE6   A0A1I8N0W4   J3JYT9   A0A088A359   A0A158NI84   E2C8L2   A0A151WR74   A0A0C9QZC0   A0A1I8PP27   A0A195EG37   F4WPR9   A0A026WIN7   B1B5K1   A0JCK6   A0A1A9VW86   A0A3L8D7J0   A0A1W4XI92   A0A0N1PH79   B4N8B1   B3NYQ7   A0A034WRL1   N6TWW7   A0A0K8WKM9   W8CBZ2   A0A0K8U524   A0A0A1WGL4   A0A034VLF9   A0A0L7QXF0   A0A195C079   A0A0A1XFE0   A0A0M9A7E2   E2AV85   A0A1Y1KZL1   A0A3S2M731   A0A0L0CE89   Q17B76   A0A1A9X740   A0A1B0FQW4   A0A1I8JTB4   T1DTQ9   A0A0K8VB28   A0A182XUW4   A0A1Y1K9U3   A0A182VAF6   Q9Y1K6   A0A034V075   Q7QKL2   A0A0A1X7H3   A0A1Y1L1S6   A0A195AWL1   A0A1W4VMD7   W8BLL3   B3F5B1   A0A0A1XAL3   A0A1A9Z167   A0A182IIR9   A0A023EQV6   A0A182G580   A0A1Y1K3W7   A0A336LMT0   Q7QB73  

PDB

2XXL     E-value=5.88573e-43,     Score=439

KEGG

692368    no KO assigned | (RefSeq) PPAE, BmCLIP1, BmPPAE, CLIP1; prophenoloxidase activating enzyme

GO

GO:0004175   GO:0004252   GO:0008233   GO:0016485   GO:0046872   GO:0016021   GO:0008236   GO:0035006   GO:0035008   GO:0006508   GO:0005615   GO:0005840   GO:0006412   GO:0033557   GO:0005839   GO:0051603   GO:0046835   GO:0006281   GO:0006259  

SignalP

Position:   1 - 21,         Likelihood:  0.998347

 

 

Length:

441

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.25092

Exp number, first 60 AAs:

0.24135

Total prob of N-in:

0.01256

outside

1  -  441

 

 

Pi

215.33969

Theta

165.337877

Tajima's D

0.591947

CLR

0.802715

CSRT

0.542222888855557

Interpretation

Uncertain