Gene

KWMTBOMO16366

Pre Gene Modal

BGIBMGA013797

Annotation

PREDICTED:_serine_protease_easter-like_[Bombyx_mori]

Location

Bomo_Chr28(+):7313883-7337797      View in EpiBrowser

Full name

Phenoloxidase-activating enzyme       + More
Melanization protease 1      
Serine protease easter      

Alternative Name

Prophenoloxidase-activating enzyme

Location in the cell

Extracellular   Reliability : 3.618

CDS

ATGTCTCCGTTGTTGTTTACGACAATCGTAGCGGCTTATTTATGCGGCGCAAACGGACAGTCAGCGTGCACGGCCTCCGGTGGCGGTCAGGGAACGTGTGTGGATATCTACAAATGCGAGCCATTGCTGGCCCTCGTTAGAAAGCCTGATCGTACTCAACAGGATCTGGAAGCTCTCAAGAAATCTCAATGCGGGTTTCAGGGAGGGAAGCCATTGGTATGCTGTCCAAGTGAATGTCACACACCGGATGGTAAACCAGGAAAATGTATAAACATATACTCTTGCGAACACCTCACTCAAATGTTGAGGCCTCCGACCTCGAAGGAAAACATGTTATACGTACAAAACTCCAGGTGTAAAAGTGCAGATCAATACAGTGTATGCTGTGGCCCAGCGCCTTTAGCCACACCCCCTCCTCCAGCGGGCAGCTGCAGGGCGACTGTTTCGGCATTACTACCCAACCCAGCGGACTCTTGCTGTGGTCTAGACGCCAACGTTGGAAACAAAATCGTTGGTGGAACAGCGACTGCTATCGACCAATACCCGTGGCTGATAGTCATCGAGTATGTCAGTGATAACAGGATCAAATTATTGTGTGGTGGCTCGCTGATAAGCGGTAGATATGTTCTTACAGCTGGCCACTGCATAGCCGGAGCGGTCCTTCAAATCGGAAAACCTACCAACATTAGACTCGGCGAATACGACACTGCTAACAGCGGACCGGATTGTGTGGTTGTCGAAGCCGGAGGCACGGACTGCACGGAAGATATAGTAGTCATACCCATTGAACAGATCATACCCCATCCGGAATATAATCCTGTCAGCCCTCAAAGGAAGAACGATATCGCTTTGATCAGAATGAGTCAAATGGCTCCTTATACTGATTTTATCAGACCGATCTGCCTTCCATCGGCGGACATTACAGTGAACACACCAAATAAAACAGTATTCTACGCCGCCGGTTGGGGTGCAGTGAGCACGAACCAGATCAGCAGCGATGTCAAATCCCACGTAGACTTGCCTTACATTCCCCACAGTATTTGCCAACCAGCGTACTCGGTGCAAAACCGCAAAATAACTTTATGGAACAAACAGATTTGCGCTGGCGGTGTTCCGGGCAAAGACTCTTGCAAGGGCGACTCCGGTGGGCCATTGATGTACGAGAACGGTAGACAATATGAAGTCGTCGGTGTGGTCAGTTTTGGCCCAACGCCGTGCGGTCTTCCCGACATTCCGGGCGTTTACACCAAAGTCTACGAATACCTCGACTGGATCAGGAGCACGGTCAAATCATAG

Protein

MSPLLFTTIVAAYLCGANGQSACTASGGGQGTCVDIYKCEPLLALVRKPDRTQQDLEALKKSQCGFQGGKPLVCCPSECHTPDGKPGKCINIYSCEHLTQMLRPPTSKENMLYVQNSRCKSADQYSVCCGPAPLATPPPPAGSCRATVSALLPNPADSCCGLDANVGNKIVGGTATAIDQYPWLIVIEYVSDNRIKLLCGGSLISGRYVLTAGHCIAGAVLQIGKPTNIRLGEYDTANSGPDCVVVEAGGTDCTEDIVVIPIEQIIPHPEYNPVSPQRKNDIALIRMSQMAPYTDFIRPICLPSADITVNTPNKTVFYAAGWGAVSTNQISSDVKSHVDLPYIPHSICQPAYSVQNRKITLWNKQICAGGVPGKDSCKGDSGGPLMYENGRQYEVVGVVSFGPTPCGLPDIPGVYTKVYEYLDWIRSTVKS

Description

Endopeptidase with selective post-Arg cleavage site (PubMed:4197814, PubMed:4197815, PubMed:10066809, PubMed:7981662). Activates prophenoloxidase (PubMed:4197814, PubMed:4197815). Has a probable role in the melanization process as part of the innate immune response (Probable).
Serine protease which plays an essential role in the melanization immune response by acting downstream of sp7 to activate prophenoloxidase (PPO1) (PubMed:16861233). May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea (PubMed:18854145, PubMed:16861233). Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway (PubMed:16861233).
Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo (PubMed:9477324, PubMed:2107028, PubMed:12493753). Three proteases; ndl, gd and snk process easter to create active easter (PubMed:9477324). Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo (PubMed:9477324).

Biophysicochemical Properties

2.2 uM for prophenoloxidase (at pH 7.6 and 0 degrees Celsius)
1.43 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the absence of KCl)
0.77 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the presence of 0.025 M KCl)
1.2 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the presence of 0.004 M KCl)
0.35 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the presence of 0.5 M KCl)
0.145 mM for N-benzoyl-L-arginine ethyl ester hydrochloride (BAEE) (in the presence of 0.5 M KCl)
0.709 mM for N-p-tosyl-L-arginine methyl ester hydrochloride (TAME) (in the presence of 0.5 M KCl)

Subunit

In the active form, heterodimer of a light chain and a heavy chain; disulfide-linked.

Similarity

Belongs to the peptidase S1 family.
Belongs to the peptidase S1 family. CLIP subfamily.

Keywords

Calcium   Complete proteome   Direct protein sequencing   Disulfide bond   Glycoprotein   Hydrolase   Metal-binding   Protease   Pyrrolidone carboxylic acid   Reference proteome   Serine protease   Signal   Zymogen   Alternative splicing   Developmental protein   Secreted  

Feature

chain  Phenoloxidase-activating enzyme light chain
propeptide  Activation peptide
splice variant  In isoform A.

Uniprot

Q6Y2X4   Q5DI98   A0A2A4JWX6   A0A290U627   Q49QW0   A0A126U9N5   + More
H9JW83   I4DJE3   A0A140EQ25   S4PHS5   A0A0N1INY1   A0A0N1IMQ8   A0A212EUU4   Q9XXV0   A0A0U1WT40   A0A212F8R3   A0A173GPF0   Q2FAY5   Q8I917   A1IIA5   I4DS78   Q5MPC0   Q2FAY6   Q2FAY4   Q2FAY7   Q5MPC2   A0A0L7L0F2   A0A126GUP6-3   A0A126GUP6-2   A0A173GPF7   A0A126GUP6   D2XPX4   W8CBZ2   B3P1Y6   A0A3B0K246   A0A3B0K4S8   A0A154P5I7   A0A1W4VLE8   A0A1J1J5N3   A0A1Y1L1S6   V5GX21   A0A2A3EA55   B4QUZ8   B0WQE7   J3JXM3   A0A034WRL1   A0A1I8PP27   A0A1I8NE97   A0A0A1WGL4   Q17B76   A0A182G580   A0A023EQV6   B3P0U7   B4PTQ4   A0A1Q3FHX1   A0A0M4EWB3   A0A0K8WKM9   Q295E4   A0A1I8N0W4   A0A0K8U524   A0A034VLF9   A0A182G5Z0   P13582   A0A034V0K7   K7J980   A0A336LMT0   A0A0K8VF53   B3MSM9   B4GM57   B4MBJ7   A0A1W4VIJ6   A0A0A1X7H3   A0A182SX76   B4QYJ5   B4KCX7   E2C8L2   A0A1B0B4Z6   D3TMC5   B4HDX9   A0A1B0FFU3   A0A3L8D7J0   A0A026WIN7   Q5MPC6   A0A1A9YQ00   B4PS51   A0A034V075   A0A0A1WU83   A0A1Y1LA41   A0A1A9UFJ1   A0A1Y1LA48   A0A1Y1K3W7   A0A3B0KH63   A0A0C9QZC0   B4N7Z2   A0A182IIR9   Q7QB73   A0A2Y9D4S9   A0A1Q3FC43  

EC Number

3.4.21.-

Pubmed

14505699    16033436    28953952    19121390    22651552    23622113    + More
26354079    22118469    10066809    4197814    4197815    7981662    16324822    12456683    17307003    15944088    26227816    10731132    12537572    10731138    16861233    18854145    22227521    24495485    17994087    28004739    22516182    25348373    25315136    25830018    17510324    26483478    24945155    17550304    15632085    2492450    9477324    9533958    2107028    12493753    20075255    20798317    20353571    30249741    24508170    12364791    14747013    17210077   

EMBL

AY188445    AAO74570.1    AH014670    AY789466    AAX18637.1    NWSH01000467    + More
PCG76208.1    KY680241    ATD13322.1    AY677082    AAW24481.1    KT751522    AML39475.1    BABH01025543    BABH01025544    AK401411    BAM18033.1    KT751521    AML39474.1    GAIX01000374    JAA92186.1    KQ460936    KPJ10700.1    KQ459547    KPJ00023.1    AGBW02012316    OWR45231.1    AB009670    BABH01025554    BAA76308.1    JQ581598    AFX82594.1    AGBW02009696    OWR50099.1    KT006138    ANH58163.1    DQ115323    AAZ91696.1    AY077643    AAL76085.1    AB264091    BAF43530.1    AK405456    BAM20768.1    AY672790    AAV91012.1    AAZ91695.1    DQ115324    AAZ91697.1    AAZ91694.1    AY672788    AAV91010.1    JTDY01003981    KOB68749.1    AE014297    AF145611    BT125977    AAD38586.1    ADX35956.1    KT006137    ANH58162.1    GU229936    ADA84072.3    GAMC01000094    JAC06462.1    CH954181    EDV47759.1    OUUW01000014    SPP88304.1    SPP88303.1    KQ434809    KZC06390.1    CVRI01000070    CRL07282.1    GEZM01066955    JAV67623.1    GALX01002154    JAB66312.1    KZ288322    PBC28172.1    CM000364    EDX11579.1    DS232039    EDS32818.1    BT127992    AEE62954.1    GAKP01002127    JAC56825.1    GBXI01016739    GBXI01006351    JAC97552.1    JAD07941.1    CH477325    EAT43491.1    JXUM01042955    JXUM01042956    KQ561344    KXJ78893.1    GAPW01002093    JAC11505.1    EDV48995.1    CM000160    EDW95637.1    GFDL01007911    JAV27134.1    CP012526    ALC47792.1    GDHF01000894    JAI51420.1    CM000070    EAL28768.2    GDHF01030580    JAI21734.1    GAKP01015995    JAC42957.1    JXUM01044209    JXUM01044210    KQ561391    KXJ78725.1    J03154    BT009978    GAKP01022176    JAC36776.1    AAZX01002213    UFQT01000091    SSX19534.1    GDHF01015149    JAI37165.1    CH902623    EDV30269.1    CH479185    EDW37931.1    CH940656    EDW58468.1    GBXI01007200    JAD07092.1    EDX12840.1    CH933806    EDW16999.2    GL453666    EFN75742.1    JXJN01008576    EZ422577    ADD18853.1    CH480815    EDW42071.1    CCAG010004727    QOIP01000012    RLU16063.1    KK107185    EZA55838.1    AY672784    AAV91006.1    EDW97471.1    GAKP01023416    JAC35542.1    GBXI01012101    JAD02191.1    GEZM01061363    JAV70514.1    GEZM01061360    JAV70519.1    GEZM01097973    JAV54026.1    OUUW01000013    SPP87780.1    GBYB01001065    JAG70832.1    CH964232    EDW81243.1    APCN01002556    AAAB01008880    EAA08507.4    GFDL01009899    JAV25146.1   

Proteomes

UP000218220    UP000005204    UP000053240    UP000053268    UP000007151    UP000037510    + More
UP000000803    UP000008711    UP000268350    UP000076502    UP000192221    UP000183832    UP000242457    UP000000304    UP000002320    UP000095300    UP000095301    UP000008820    UP000069940    UP000249989    UP000002282    UP000092553    UP000001819    UP000002358    UP000007801    UP000008744    UP000008792    UP000075901    UP000009192    UP000008237    UP000092460    UP000001292    UP000092444    UP000279307    UP000053097    UP000092443    UP000078200    UP000007798    UP000075840    UP000007062   

PRIDE

H9JW83    Q9XXV0    A0A126GUP6    P13582   

Pfam

PF12032   CLIP        + More
PF00089   Trypsin
PF13019   Sde2_N_Ubi

Interpro

IPR033116   TRYPSIN_SER        + More
IPR022700   CLIP       
IPR038565   CLIP_sf       
IPR001254   Trypsin_dom       
IPR018114   TRYPSIN_HIS       
IPR009003   Peptidase_S1_PA       
IPR001314   Peptidase_S1A       
IPR029071   Ubiquitin-like_domsf       
IPR024974   Sde2_N       

SUPFAM

SSF50494   SSF50494        + More
SSF54236   SSF54236       

Gene 3D

2.20.20.90    

CDD

cd00190  Tryp_SPc

ProteinModelPortal

Q6Y2X4   Q5DI98   A0A2A4JWX6   A0A290U627   Q49QW0   A0A126U9N5   + More
H9JW83   I4DJE3   A0A140EQ25   S4PHS5   A0A0N1INY1   A0A0N1IMQ8   A0A212EUU4   Q9XXV0   A0A0U1WT40   A0A212F8R3   A0A173GPF0   Q2FAY5   Q8I917   A1IIA5   I4DS78   Q5MPC0   Q2FAY6   Q2FAY4   Q2FAY7   Q5MPC2   A0A0L7L0F2   A0A126GUP6-3   A0A126GUP6-2   A0A173GPF7   A0A126GUP6   D2XPX4   W8CBZ2   B3P1Y6   A0A3B0K246   A0A3B0K4S8   A0A154P5I7   A0A1W4VLE8   A0A1J1J5N3   A0A1Y1L1S6   V5GX21   A0A2A3EA55   B4QUZ8   B0WQE7   J3JXM3   A0A034WRL1   A0A1I8PP27   A0A1I8NE97   A0A0A1WGL4   Q17B76   A0A182G580   A0A023EQV6   B3P0U7   B4PTQ4   A0A1Q3FHX1   A0A0M4EWB3   A0A0K8WKM9   Q295E4   A0A1I8N0W4   A0A0K8U524   A0A034VLF9   A0A182G5Z0   P13582   A0A034V0K7   K7J980   A0A336LMT0   A0A0K8VF53   B3MSM9   B4GM57   B4MBJ7   A0A1W4VIJ6   A0A0A1X7H3   A0A182SX76   B4QYJ5   B4KCX7   E2C8L2   A0A1B0B4Z6   D3TMC5   B4HDX9   A0A1B0FFU3   A0A3L8D7J0   A0A026WIN7   Q5MPC6   A0A1A9YQ00   B4PS51   A0A034V075   A0A0A1WU83   A0A1Y1LA41   A0A1A9UFJ1   A0A1Y1LA48   A0A1Y1K3W7   A0A3B0KH63   A0A0C9QZC0   B4N7Z2   A0A182IIR9   Q7QB73   A0A2Y9D4S9   A0A1Q3FC43  

PDB

2OLG     E-value=2.4764e-45,     Score=459

KEGG

101737050    no KO assigned | (RefSeq) serine protease easter-like

GO

GO:0004252   GO:0004175   GO:0008233   GO:0016485   GO:0046872   GO:0035006   GO:0009620   GO:0006508   GO:0005615   GO:0009950   GO:0007370   GO:0045752   GO:0005576   GO:0009953   GO:0007311   GO:0098595   GO:0008063   GO:0008236   GO:0016021   GO:0005840   GO:0006412   GO:0033557   GO:0005839   GO:0051603   GO:0046835   GO:0006281   GO:0006259  

Subcellular location

Secreted  

SignalP

Position:   1 - 19,         Likelihood:  0.993707

 

 

Length:

431

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0637500000000001

Exp number, first 60 AAs:

0.02197

Total prob of N-in:

0.00245

outside

1  -  431

 

 

Pi

244.464067

Theta

159.471328

Tajima's D

1.45855

CLR

0.052049

CSRT

0.779211039448028

Interpretation

Uncertain