Gene

KWMTBOMO16362

Annotation

hemolymph_proteinase_10_[Manduca_sexta]

Location

Bomo_Chr28(-):7236862-7244192      View in EpiBrowser

Full name

CLIP domain-containing serine protease 2       + More
Serine protease easter      

Alternative Name

BzArgOEtase

Location in the cell

Extracellular   Reliability : 2.401

CDS

ATGTATAGTATAAAATTATATTTGTGCATCGCGGCGATTTGCGTATTTGTGTTTCTTGAATTCCCAAAATTTAAGGGAAATGAGCACAGATGTGGAATTGAAGCCAGCGGCAACCTTATCCATCACAACCCCTGGTTAGTCTACTTGGAGTACTACGTGGGCGATCATCAGAAGGAATACAGGTGTTCCGGCACGCTGATCAGCCCTCGGCACGTCGTAACAGCAGCTCATTGTGTCAGGAAAATAAAGTTCACCCGTCTGGCGGCTCGTCTCGGCGACTATGACGTTTCAACGGAAAAGGACTGCGTGACGGGCTTGTGCTCTGATCCTAACCTATCTATCCAAGTTTCGGAGATAATCGTCCACCCCGACTACGATGGAAAAGAAGGGGATATCGCGATTCTGAAACTGGACACGGACGCGCCATATACAGACTTCATTCGGCCGATCTGCCTTCCATCCAATCCGATTAAGGGCAACGTCACCCTTTACGCCACCGGCTGGGGGGAGATCCCCACTATAGGAATGTACAGTAATATCAAGAAGATCCTGCCATTACCCAACTGGTCTCTAGAGCAATGCCATAGAGTCTTCGAGAATCTGCCAGATAAGGTGCTCTGTGCTGGAGGCGAAGAAGGCGTTGATACTTGCCGCGGAGACTCTGGTGGTACTCTTGCTCTGGTGAAGGAGAGAGTGGAGTTCGTCGGAGTCACCAGCACTGGGTGCCAGCGATGCGGAACCAAGGGCTACCCAGCTGTATACGTTAGTGTCTATGACTACATAGATTGGATAAGCTTGGTCACGAAACACTGA

Protein

MYSIKLYLCIAAICVFVFLEFPKFKGNEHRCGIEASGNLIHHNPWLVYLEYYVGDHQKEYRCSGTLISPRHVVTAAHCVRKIKFTRLAARLGDYDVSTEKDCVTGLCSDPNLSIQVSEIIVHPDYDGKEGDIAILKLDTDAPYTDFIRPICLPSNPIKGNVTLYATGWGEIPTIGMYSNIKKILPLPNWSLEQCHRVFENLPDKVLCAGGEEGVDTCRGDSGGTLALVKERVEFVGVTSTGCQRCGTKGYPAVYVSVYDYIDWISLVTKH

Description

Endopeptidase with selective post-Arg cleavage site (PubMed:16399077, PubMed:7737188). Might play a role in innate immunity in response to fungal and Gram-positive bacterial infections (PubMed:16399077).
Component of the extracellular signaling pathway that establishes the dorsal-ventral pathway of the embryo (PubMed:9477324, PubMed:2107028, PubMed:12493753). Three proteases; ndl, gd and snk process easter to create active easter (PubMed:9477324). Active easter defines cell identities along the dorsal-ventral continuum by activating the spz ligand for the Tl receptor in the ventral region of the embryo (PubMed:9477324).

Subunit

In the active form, heterodimer of a light chain and a heavy chain; disulfide-linked.

Similarity

Belongs to the peptidase S1 family.
Belongs to the peptidase S1 family. CLIP subfamily.

Keywords

Calcium   Cleavage on pair of basic residues   Complete proteome   Direct protein sequencing   Disulfide bond   Glycoprotein   Hydrolase   Metal-binding   Protease   Reference proteome   Secreted   Serine protease   Signal   Zymogen   Developmental protein  

Feature

chain  CLIP domain-containing serine protease 2 light chain
propeptide  Activation peptide

Uniprot

A0A2H1WEH9   A0A2A4JXI6   Q5MPC4   A0A2H1W881   Q5MPC7   A0A2A4JQ35   + More
A0A3S2P3X8   Q5MGE4   A0A0T6AZ73   B4M4J5   A0A2M4DQK3   A0A1I8N0W4   W5J9Y3   I4DS78   A0A182S527   A0A2H1WUN7   A0A3F2YTZ8   A0A0L0CE89   A0A1J1J5N3   A0A1I8PP27   A0A212F949   A0A0B4KGQ4   Q7QKL2   A0A1I8JTB4   A0A023EQB0   A0A2C9GV95   A0A2M3Z9P7   A0A2M3ZA33   A0A182GNZ3   B4K6S5   D6W9Z5   B3MSM9   A0A139WN30   A0A2A4JWX6   Q9Y1K6   A0A1W7R8M0   T1DQX5   D2XPX4   A0A0L0C6E5   A0A182YKD5   E2C8L2   A0A212FEW0   A0A290U627   A0A1Q3FEM6   A0A2M4AJX7   A0A2M4BSD0   A0A2M4BSA3   A0A2M4BS12   A0A1S4F560   B3P0U7   A0A3F2YU16   A0A2M4AJZ4   A0A2C9GV93   A0A2C9GWT4   A0A182VAF6   A0A3F2YTX9   B4KCX7   I4DJE3   A0A084VUH0   Q2VG86   A0A182T262   A0A2C9GW66   Q49QW0   B4QYJ5   B0WA88   B4HDX9   A0A1W4VIJ6   C6ZQY3   A0A2M4BRQ8   Q17EX4   P13582   A0A182R026   A0A182QL12   Q17EX5   U5EHU2   A0A2M4BS19   B4PS51   A0A2M4BS35   A0A2M4BS37   W5J6E1   B5DWB5   A0A2M4AR21   W8BLL3   A0A182LCK2   A0A0N1INY1   T1DU92   A1IIA5   A0A1I8PZ22   Q294N1   B4GMV5   A0A1B0G9G5   A0A182XUS5   A0A2C9GTT7   A0A182XUW4   Q17B76   B4JS10   B4N7Z2   Q7PQR9   T1P924   A0A0A1WGL4  

EC Number

3.4.21.-

Pubmed

15944088    16023793    17994087    25315136    20920257    23761445    + More
22651552    26108605    22118469    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    12364791    14747013    17210077    24945155    26483478    18362917    19820115    10646969    25244985    20798317    28953952    17510324    24438588    16399077    19121390    7737188    20496585    2492450    9477324    9533958    2107028    12493753    17550304    15632085    24495485    20966253    26354079    17307003    25830018   

EMBL

ODYU01008137    SOQ51490.1    NWSH01000467    PCG76213.1    AY672786    AAV91008.1    + More
ODYU01006975    SOQ49305.1    AY672783    AAV91005.1    NWSH01000925    PCG73522.1    RSAL01000029    RVE51737.1    AY829842    AAV91456.1    LJIG01022469    KRT80412.1    CH940652    EDW59556.1    GGFL01015657    MBW79835.1    ADMH02002074    ETN59569.1    AK405456    BAM20768.1    ODYU01011137    SOQ56686.1    JRES01000503    KNC30561.1    CVRI01000070    CRL07282.1    AGBW02009640    OWR50264.1    AE014297    AGB95978.1    AAAB01008794    EAA03566.2    APCN01002147    GAPW01001836    JAC11762.1    GGFM01004490    MBW25241.1    GGFM01004547    MBW25298.1    JXUM01015012    JXUM01015013    KQ560420    KXJ82504.1    CH933806    EDW14191.1    KQ971312    EEZ99203.2    CH902623    EDV30269.1    KYB29266.1    PCG76208.1    AF117749    AAD38335.1    GEHC01000168    JAV47477.1    GAMD01000905    JAB00686.1    GU229936    ADA84072.3    JRES01000835    KNC27963.1    GL453666    EFN75742.1    AGBW02008891    OWR52286.1    KY680241    ATD13322.1    GFDL01009047    JAV25998.1    GGFK01007768    MBW41089.1    GGFJ01006752    MBW55893.1    GGFJ01006751    MBW55892.1    GGFJ01006726    MBW55867.1    CH954181    EDV48995.1    GGFK01007753    MBW41074.1    EDW16999.2    AK401411    BAM18033.1    ATLV01016791    KE525113    KFB41614.1    AB035418    AY061936    DQ157441    BABH01019990    AAL31707.1    ABB58762.1    BAE73254.1    AXCN02000165    AY677082    AAW24481.1    CM000364    EDX12840.1    DS231869    EDS40917.1    CH480815    EDW42071.1    EZ114934    ACU30987.1    GGFJ01006522    MBW55663.1    CH477278    EAT45085.1    J03154    BT009978    EAT45084.1    GANO01002884    JAB56987.1    GGFJ01006671    MBW55812.1    CM000160    EDW97471.1    GGFJ01006728    MBW55869.1    GGFJ01006754    MBW55895.1    ETN59571.1    CM000070    EDY68533.1    GGFK01009924    MBW43245.1    GAMC01016016    JAB90539.1    KQ460936    KPJ10700.1    GAMD01000359    JAB01232.1    AB264091    BAF43530.1    EAL28934.1    CH479185    EDW38179.1    CCAG010018107    CH477325    EAT43491.1    CH916373    EDV94550.1    CH964232    EDW81243.1    AAAB01008879    EAA08404.4    KA645202    AFP59831.1    GBXI01016739    GBXI01006351    JAC97552.1    JAD07941.1   

Proteomes

UP000218220    UP000283053    UP000008792    UP000095301    UP000000673    UP000075900    + More
UP000075881    UP000037069    UP000183832    UP000095300    UP000007151    UP000000803    UP000007062    UP000075840    UP000075884    UP000069940    UP000249989    UP000009192    UP000007266    UP000007801    UP000076408    UP000008237    UP000008711    UP000075903    UP000030765    UP000005204    UP000075901    UP000075886    UP000000304    UP000002320    UP000001292    UP000192221    UP000008820    UP000002282    UP000001819    UP000075882    UP000053240    UP000008744    UP000092444    UP000076407    UP000001070    UP000007798   

PRIDE

Q2VG86    P13582   

Pfam

PF00089   Trypsin        + More
PF12032   CLIP

Interpro

IPR018114   TRYPSIN_HIS        + More
IPR001254   Trypsin_dom       
IPR001314   Peptidase_S1A       
IPR009003   Peptidase_S1_PA       
IPR033116   TRYPSIN_SER       
IPR038565   CLIP_sf       
IPR022700   CLIP       

SUPFAM

SSF50494   SSF50494       

Gene 3D

2.20.20.90    

CDD

cd00190  Tryp_SPc

ProteinModelPortal

A0A2H1WEH9   A0A2A4JXI6   Q5MPC4   A0A2H1W881   Q5MPC7   A0A2A4JQ35   + More
A0A3S2P3X8   Q5MGE4   A0A0T6AZ73   B4M4J5   A0A2M4DQK3   A0A1I8N0W4   W5J9Y3   I4DS78   A0A182S527   A0A2H1WUN7   A0A3F2YTZ8   A0A0L0CE89   A0A1J1J5N3   A0A1I8PP27   A0A212F949   A0A0B4KGQ4   Q7QKL2   A0A1I8JTB4   A0A023EQB0   A0A2C9GV95   A0A2M3Z9P7   A0A2M3ZA33   A0A182GNZ3   B4K6S5   D6W9Z5   B3MSM9   A0A139WN30   A0A2A4JWX6   Q9Y1K6   A0A1W7R8M0   T1DQX5   D2XPX4   A0A0L0C6E5   A0A182YKD5   E2C8L2   A0A212FEW0   A0A290U627   A0A1Q3FEM6   A0A2M4AJX7   A0A2M4BSD0   A0A2M4BSA3   A0A2M4BS12   A0A1S4F560   B3P0U7   A0A3F2YU16   A0A2M4AJZ4   A0A2C9GV93   A0A2C9GWT4   A0A182VAF6   A0A3F2YTX9   B4KCX7   I4DJE3   A0A084VUH0   Q2VG86   A0A182T262   A0A2C9GW66   Q49QW0   B4QYJ5   B0WA88   B4HDX9   A0A1W4VIJ6   C6ZQY3   A0A2M4BRQ8   Q17EX4   P13582   A0A182R026   A0A182QL12   Q17EX5   U5EHU2   A0A2M4BS19   B4PS51   A0A2M4BS35   A0A2M4BS37   W5J6E1   B5DWB5   A0A2M4AR21   W8BLL3   A0A182LCK2   A0A0N1INY1   T1DU92   A1IIA5   A0A1I8PZ22   Q294N1   B4GMV5   A0A1B0G9G5   A0A182XUS5   A0A2C9GTT7   A0A182XUW4   Q17B76   B4JS10   B4N7Z2   Q7PQR9   T1P924   A0A0A1WGL4  

PDB

2OLG     E-value=2.23805e-35,     Score=371

KEGG

101736559    no KO assigned | (RefSeq) serine protease easter-like

GO

GO:0004252   GO:0008236   GO:0035006   GO:0035008   GO:0006508   GO:0005615   GO:0004175   GO:0005576   GO:0046872   GO:0009950   GO:0007370   GO:0045752   GO:0009953   GO:0007311   GO:0098595   GO:0008063   GO:0005840   GO:0006412   GO:0033557   GO:0005839   GO:0051603   GO:0046835   GO:0006281   GO:0006259  

Subcellular location

Secreted   Secreted in the hemolymph.   With evidence from 6 publications.

SignalP

Position:   1 - 26,         Likelihood:  0.755356

 

 

Length:

270

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

9.79067999999998

Exp number, first 60 AAs:

9.75252000000001

Total prob of N-in:

0.46817

outside

1  -  270

 

 

Pi

32.855533

Theta

13.917719

Tajima's D

-1.234908

CLR

39.599476

CSRT

0.0983450827458627

Interpretation

Uncertain