Gene

KWMTBOMO16267  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA013895

Annotation

5'-methylthioadenosine_phosphorylase_[Bombyx_mori]

Location

Bomo_Chr28(-):3284183-3292667      View in EpiBrowser

Full name

S-methyl-5'-thioadenosine phosphorylase       + More
Purine nucleoside phosphorylase      

Alternative Name

5'-methylthioadenosine phosphorylase

Location in the cell

Cytoplasmic   Reliability : 2.21

CDS

ATGGGCGATGTAAGAAAAATAAAGATCGGAATTATCGGAGGTTCGGGCTTCGATGATCCGACGCTTTTCGAGAATCAAATCGAAAAGGAGGTGGTCACACCGTTTGGGAGACCCTCGGACGTACTGATAGAGGGTCAAATCAAACGGGTCCAATGCGTTCTGCTGGCACGACACGGCAGAAAACATCAACTTCAACCTAGCGATGTGAACTATCGAGCTAATATTTGGGCACTGAAGCAAGTCGGCTGCACTCACATCCTCGCCACGACCGCCACTGGGTCTCTAGTTGAAGAATACAGGCCTGGGGATCTGGTCATATTGGACGATTTCATTGATAGGACTTGGGGTCGGAAGTGCACGTTTTACGACAACACGGAGGGGGGTCCGCGCGGCGTGTGCCACCTGCCCATGCGGCCGGCGTATTGTGGGAGAGCGCGCGCCGCACTGTACAGCGCGGCCAAGTCTAGGGGGTACTCGTGCCATGAAACCGGCACTGCGGTTGTCATACAGGGACCCAGATTCTCAAGTCGCGCCGAAAGCTTGGTCCATAGACAATGGGGTGGCCATCTTGTGAACATGACAACTGTCCCTGAGGTTGTGTTAGCTAAAGAAGCCGGCTTGAGTTACGCTGCCGTGGCACTAGTCACAGATTACGATTGTTGGAGGGAAAATGAAAAATCCGTCTCGGTGAGCGAAGTTCTGGCAACATTCTCGAAGAACGTTAAGAAGGCTGCGGATGTTATCGTAGACGCAGTTCAGATATTAGGAGCCGATACCGACCTCCAATATCTGGACGATCACCAGGAACTCGTCAAATCCGCCATAATGCTCAAGGACTGA

Protein

MGDVRKIKIGIIGGSGFDDPTLFENQIEKEVVTPFGRPSDVLIEGQIKRVQCVLLARHGRKHQLQPSDVNYRANIWALKQVGCTHILATTATGSLVEEYRPGDLVILDDFIDRTWGRKCTFYDNTEGGPRGVCHLPMRPAYCGRARAALYSAAKSRGYSCHETGTAVVIQGPRFSSRAESLVHRQWGGHLVNMTTVPEVVLAKEAGLSYAAVALVTDYDCWRENEKSVSVSEVLATFSKNVKKAADVIVDAVQILGADTDLQYLDDHQELVKSAIMLKD

Description

Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.
Purine nucleoside phosphorylase involved in purine salvage.

Catalytic Activity

phosphate + S-methyl-5'-thioadenosine = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate
a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha-D-ribose 1-phosphate

Subunit

Homotrimer.

Miscellaneous

Although this enzyme belongs to the family of MTA phosphorylases based on sequence homology, it lacks several conserved amino acids in the substrate binding pocket that confer specificity towards MTA.

Similarity

Belongs to the PNP/MTAP phosphorylase family. MTAP subfamily.

Keywords

Complete proteome   Cytoplasm   Glycosyltransferase   Nucleus   Purine salvage   Reference proteome   Transferase   Acetylation  

Feature

chain  S-methyl-5'-thioadenosine phosphorylase

Uniprot

Q1HQ82   H9JWI1   A0A2A4K4C7   A0A2W1B6H2   I4DLI4   A0A0N1PH14   + More
A0A194PWJ7   A0A212FEM5   A0A220K8J9   S4PSF7   A0A3S2M6L3   A0A336LVV3   D6WPA2   A0A1L8DXQ8   T1P8V4   A0A1I8N9R6   V5GMI9   A0A1Y1MEH6   A0A1W4XN90   E3TFK1   A0A093Q5T8   B2GQM6   Q7ZV22   A0A3B3ZNI1   F1NCV7   A0A2I4D6S4   A0A3Q0T2T2   A0A091IS19   A0A3B3BKU3   A0A3P9LHK0   A0A093IKE2   A0A087RD82   A0A182M5A4   H0WX98   A0A182SMW3   G1T2Z8   A0A182N801   A0A182PHE2   A0A182YNS0   F7AA57   A0A093I3N3   A0A3B4Z2M5   A0A2U3ZTG6   A0A091V1J1   A0A087VKB9   A0A1W4YT55   A0A2U3WU82   G3T3Y4   A0A099ZW69   A0A091VGE7   A0A091L1G8   A0A182VIQ8   A0A182K140   A0A182LBD4   A0A182XJA1   A0A182HP61   Q7Q9N9   A0A3L7I8P3   Q9CQ65   U6CT37   A0A2Y9L3N6   M3YX12   A0A182RTP8   A0A091MTI6   A0A091P836   A0A1B6CHF1   A0A093DEC8   A0A093FXA3   A0A091ERY4   A0A3B4XVF1   A0A093P8F8   I3KLX3   A0A2U3YP82   A0A383ZPP0   A0A3B4TAR6   A0A3Q0CS45   Q6FHT1   A0A3B4FPQ9   A0A3P8QNY6   A0A1A8PQS3   A0A1A8MHI1   G1LLW4   A0A3S2PTX5   A0A1A8F492   A0A2K6DWP1   A0A2I2UPB1   F6RQM9   I7GJ70   A0A3P9CWM5   A0A3Q7MNR7   A0A1I7Q457   A0A250YEG0   A0A099ZIL1   A0A3Q1ING3   Q5ZHQ7   A0A093DXQ9   A0A0G2K583   A0A0A1X9J3   A0A2K6M620  

EC Number

2.4.2.28
2.4.2.1

Pubmed

19121390    28756777    22651552    26354079    22118469    28630352    + More
23622113    18362917    19820115    25315136    28004739    20634964    23594743    25463417    15592404    29451363    17554307    21993624    25244985    17495919    20966253    12364791    29704459    12824877    16141072    15489334    21183079    23806337    25186727    26319212    28071753    20010809    17975172    17431167    17194215    28087693    15642098    15057822    25830018   

EMBL

DQ443170    ABF51259.1    BABH01018616    BABH01018617    NWSH01000142    PCG79115.1    + More
KZ150355    PZC71238.1    AK402152    BAM18774.1    KQ460417    KPJ14874.1    KQ459588    KPI97746.1    AGBW02008913    OWR52191.1    MF319699    ASJ26413.1    GAIX01013868    JAA78692.1    RSAL01000025    RVE52156.1    UFQS01000015    UFQT01000015    SSW97362.1    SSX17748.1    KQ971354    EFA07386.2    GFDF01002890    JAV11194.1    KA645206    AFP59835.1    GALX01005644    JAB62822.1    GEZM01036390    JAV82735.1    GU589135    ADO29087.1    KL416278    KFW81805.1    BC164800    AAI64800.1    BX323448    BC046035    BC056545    AC195266    KL218126    KFP02436.1    KK589556    KFV99243.1    KL226301    KFM11436.1    AXCM01001928    AAQR03014015    AAQR03014016    AAQR03014017    AAQR03014018    AAQR03014019    AAQR03014020    AAGW02033076    AAGW02033077    AAGW02033078    AAGW02033079    KL214741    KFV61351.1    KK409286    KFQ83628.1    KL496516    KFO13061.1    KL869929    KGL86529.1    KK733937    KFR01538.1    KL291445    KFP49637.1    APCN01002921    AAAB01008900    RAZU01000092    RLQ73941.1    AB056100    AK005064    AK011421    AK167319    BC003858    HAAF01001820    CCP73646.1    AEYP01053750    KK837640    KFP80823.1    KL381615    KFP87675.1    GEDC01024439    JAS12859.1    KN125705    KFU83838.1    KK399278    KFV59011.1    KK718913    KFO59781.1    KL225291    KFW70535.1    AERX01027956    AERX01027957    FJ224338    CR541670    ACI46030.1    CAG46471.1    HAEH01008106    HAEI01009746    SBR83603.1    HAEF01014660    HAEG01003409    SBR55819.1    ACTA01169101    ACTA01177099    CM012437    RVE76207.1    HAEB01007633    HAEC01010783    SBQ54160.1    AANG04001372    JSUE03014969    JSUE03014970    AQIA01024519    AQIA01024520    AQIA01024521    AQIA01024522    AB174101    BAE91163.1    GFFW01002775    JAV42013.1    KL893441    KGL80818.1    AJ721077    CAG32736.1    KL452767    KFV06966.1    AABR07049152    GBXI01006897    JAD07395.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000053268    UP000007151    UP000283053    + More
UP000007266    UP000095301    UP000192223    UP000221080    UP000000437    UP000261520    UP000000539    UP000192220    UP000261340    UP000054308    UP000261560    UP000265180    UP000265200    UP000053286    UP000075883    UP000005225    UP000075901    UP000001811    UP000075884    UP000075885    UP000076408    UP000002280    UP000053875    UP000261400    UP000245340    UP000192224    UP000007646    UP000053858    UP000053605    UP000075903    UP000075881    UP000075882    UP000076407    UP000075840    UP000007062    UP000273346    UP000000589    UP000248482    UP000000715    UP000075900    UP000052976    UP000261360    UP000054081    UP000005207    UP000245341    UP000261681    UP000261420    UP000189706    UP000261460    UP000265100    UP000008912    UP000233120    UP000011712    UP000006718    UP000233100    UP000265160    UP000286641    UP000053641    UP000265040    UP000002494    UP000233180   

PRIDE

Q1HQ82    H9JWI1    Q9CQ65    I3KLX3    Q6FHT1    A0A0G2K583    + More

Pfam

PF01048   PNP_UDP_1        + More
PF08238   Sel1

Interpro

IPR018099   Purine_phosphorylase-2_CS        + More
IPR010044   MTAP       
IPR000845   Nucleoside_phosphorylase_d       
IPR035994   Nucleoside_phosphorylase_sf       
IPR006597   Sel1-like       
IPR011990   TPR-like_helical_dom_sf       

SUPFAM

SSF53167   SSF53167       

Gene 3D

3.40.50.1580     1.25.40.10    

ProteinModelPortal

Q1HQ82   H9JWI1   A0A2A4K4C7   A0A2W1B6H2   I4DLI4   A0A0N1PH14   + More
A0A194PWJ7   A0A212FEM5   A0A220K8J9   S4PSF7   A0A3S2M6L3   A0A336LVV3   D6WPA2   A0A1L8DXQ8   T1P8V4   A0A1I8N9R6   V5GMI9   A0A1Y1MEH6   A0A1W4XN90   E3TFK1   A0A093Q5T8   B2GQM6   Q7ZV22   A0A3B3ZNI1   F1NCV7   A0A2I4D6S4   A0A3Q0T2T2   A0A091IS19   A0A3B3BKU3   A0A3P9LHK0   A0A093IKE2   A0A087RD82   A0A182M5A4   H0WX98   A0A182SMW3   G1T2Z8   A0A182N801   A0A182PHE2   A0A182YNS0   F7AA57   A0A093I3N3   A0A3B4Z2M5   A0A2U3ZTG6   A0A091V1J1   A0A087VKB9   A0A1W4YT55   A0A2U3WU82   G3T3Y4   A0A099ZW69   A0A091VGE7   A0A091L1G8   A0A182VIQ8   A0A182K140   A0A182LBD4   A0A182XJA1   A0A182HP61   Q7Q9N9   A0A3L7I8P3   Q9CQ65   U6CT37   A0A2Y9L3N6   M3YX12   A0A182RTP8   A0A091MTI6   A0A091P836   A0A1B6CHF1   A0A093DEC8   A0A093FXA3   A0A091ERY4   A0A3B4XVF1   A0A093P8F8   I3KLX3   A0A2U3YP82   A0A383ZPP0   A0A3B4TAR6   A0A3Q0CS45   Q6FHT1   A0A3B4FPQ9   A0A3P8QNY6   A0A1A8PQS3   A0A1A8MHI1   G1LLW4   A0A3S2PTX5   A0A1A8F492   A0A2K6DWP1   A0A2I2UPB1   F6RQM9   I7GJ70   A0A3P9CWM5   A0A3Q7MNR7   A0A1I7Q457   A0A250YEG0   A0A099ZIL1   A0A3Q1ING3   Q5ZHQ7   A0A093DXQ9   A0A0G2K583   A0A0A1X9J3   A0A2K6M620  

PDB

6DZ3     E-value=5.06313e-81,     Score=765

KEGG

733102    K00772 5'-methylthioadenosine phosphorylase [EC:2.4.2.28] | (RefSeq) Mtap; methylthioadenosine phosp

PATHWAY

00270    Cysteine and methionine metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0019509   GO:0017061   GO:0005737   GO:0006166   GO:0005634   GO:0005829   GO:0009116   GO:0032259   GO:0033574   GO:0003824   GO:0016763   GO:0005515   GO:0005839   GO:0051603   GO:0046835   GO:0006281   GO:0006259  

PANTHER

PTHR42679  

Subcellular location

Cytoplasm  
Nucleus  

Length:

279

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0195

Exp number, first 60 AAs:

0.00014

Total prob of N-in:

0.07922

outside

1  -  279

 

 

Pi

173.888615

Theta

165.857413

Tajima's D

0.269888

CLR

175.908642

CSRT

0.448177591120444

Interpretation

Uncertain