Gene

KWMTBOMO16257

Pre Gene Modal

BGIBMGA013994

Annotation

PREDICTED:_juvenile_hormone_epoxide_hydrolase-like_protein_3_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr28(+):3191858-3199535      View in EpiBrowser

Full name

Glutathione peroxidase       + More
Epoxide hydrolase      
Juvenile hormone epoxide hydrolase      
Juvenile hormone epoxide hydrolase 1      

Alternative Name

CfEH1
Juvenile hormone epoxide hydrolase I
Juvenile hormone-specific epoxide hydrolase I

Location in the cell

Cytoplasmic   Reliability : 1.675 Mitochondrial   Reliability : 1.039

CDS

ATGAATTCCAACGAAAAAAATAAGTCATTCAAATCATTTATTCTCATCACTGTCATACAAGTGCTCAGCGCATATTATCTGTATCGCTTTTTCTTCGTACCACCTGATATGCCAGCACTTGACACTGAACAATGGTGGGGCCCGTACCCTATGGACTTGGACCACGATAAATCAATAAGACCGCTAACGATAGAATTTTCTGATGTGATGATAAACGATCTACGCGAGCGACTTCTCCACCGAAGACCCCTACAGCCACCCTTAGAAAATGTTGGATTCACATACGGCTTCAACCCAAGATATTTAAACCAAATAATGGAGTATTGGCATAACAAATACAACTTTAAAGAGAGGGAACAGTTTTTGAACCAATACGATCACTTTGTAACCAATATCCAGGGTTTGGACATACATTTCATGCATATAAAACCTAAGAACATTGCTGGTCTTGAGGTGGTTCCTCTAATCATGCTGCATGGATGGCCCGGTTCATTCAGGGAATTCTATGATGTCATCCCATATTTAATGGCAGAACAGCCAGAACAAAAAATCGCTTTTGAAATCATCGTGCCAAGCTTACCCGGTTTTGGTTACTCACAGGCCTCGGTGAGACCAGGTTTGGGTCCAGCTGAAATGGCGGTGATCATCAACAATCTCATGAAGAGGATCGGCCATGATAAATATTACATACAAGGTGGAGACCTAGGGCACACTGTTGGCTCCATCATTGCTACTGCATTCCCTGAAAACGTATTAGGATTTCACACAAATTTCCCAGTGCTTCTATCTCATAGAGCTGCTCTCTTATATATAACTTGTGGCTCTTTGATTCCATCTCTAATTGAAACGAAAGAATTGCAACCCCGCCTGTACCCGCTATCCGAGCACTGGTCTAGACTCATTGAGGAGTCAGGGTACATGCACATTCAAGCCACAAAACCCGAGACTGTCGGTGTGGGACTATCAGACTCGCCAGCAGGCCTAGCAGCCTACATTCTAGAAAAGTTTTCAACTTGGACAAATCCTGATAACAAGAAGGCCGGTGACGGAAACCTCTTGAAGAAGTTCTCTTTGAATCAGCTATTGGATAATCTGATGATATACTGGGCCACTAACACTGTCACAACCAGCATGAGGGTGTATGCTGAATCATTTAATGAAAAACAAAACTTTCTTATTTTCGACAGGATACCAACAAATGTTCCGACGTGGGGTATAAAATTCAAGTATGAACTGATGTACCAACCGGACACAGCATTAAGTTTCAAATACAAGAAGTACTTGCAGAGCACTGTGGTGGAGGACGGTGGTCACTTCGCTGCCATGGAATATCCTGAATTGATGGCCAAAGATATCATACAAGCTGTGATTAAATTTCAAAAATTTTGGAATTTCGAAAAACAAGAATCTAACGAAACGCCGCCTCTCCACGAGACCGCGAGAACAGTATACGAATTTACAGTTAAATCAATAAACGGACGCGACGTCAAGCTGTCAGATTACAAAGGGAACGTGCTGCTCATTGTCAATGTAGCCTCTCAGTGCGGCCTTACGGCCACCAACTATCAACAACTCAACGAGCTACACGAAAAGTACCACCAGAAAGGTCTACGTATACTGGCCTTCCCGTGCAACCAGTTCAACGGCCAAGAACCAGGTACAGCTAAGGACATTCTGAACTTCACAAAAGACAGAGGCGTTAAATTTGATCTGTTTGAAAAAGTCGATGTGAACGGTGACAACGCGCATCCGCTTTGGAAGTTTCTGAAGAAGGCGCAGAGCGGGACGATTGGTGATTTTATCAAGTGGAACTTTAGTAAGTTCGTAGTCGACAGAAACGGCGTGCCCGTCGAGAGATACGCGCCGCATGTCAATCCACTGGATCTGGAAAAGGATTTGGCCAAATATTGGTAA

Protein

MNSNEKNKSFKSFILITVIQVLSAYYLYRFFFVPPDMPALDTEQWWGPYPMDLDHDKSIRPLTIEFSDVMINDLRERLLHRRPLQPPLENVGFTYGFNPRYLNQIMEYWHNKYNFKEREQFLNQYDHFVTNIQGLDIHFMHIKPKNIAGLEVVPLIMLHGWPGSFREFYDVIPYLMAEQPEQKIAFEIIVPSLPGFGYSQASVRPGLGPAEMAVIINNLMKRIGHDKYYIQGGDLGHTVGSIIATAFPENVLGFHTNFPVLLSHRAALLYITCGSLIPSLIETKELQPRLYPLSEHWSRLIEESGYMHIQATKPETVGVGLSDSPAGLAAYILEKFSTWTNPDNKKAGDGNLLKKFSLNQLLDNLMIYWATNTVTTSMRVYAESFNEKQNFLIFDRIPTNVPTWGIKFKYELMYQPDTALSFKYKKYLQSTVVEDGGHFAAMEYPELMAKDIIQAVIKFQKFWNFEKQESNETPPLHETARTVYEFTVKSINGRDVKLSDYKGNVLLIVNVASQCGLTATNYQQLNELHEKYHQKGLRILAFPCNQFNGQEPGTAKDILNFTKDRGVKFDLFEKVDVNGDNAHPLWKFLKKAQSGTIGDFIKWNFSKFVVDRNGVPVERYAPHVNPLDLEKDLAKYW

Description

Catalyzes juvenile hormone hydrolysis.
Catalyzes juvenile hormone hydrolysis. Degrades juvenile hormone III (JH III) about 3 times and 5 times slower than juvenile hormone I (JH I) and II (JH II), respectively. Degrades cis-stilbene oxide and trans-stilbene oxide about 18 and 43 times slower than JH III, respectively.

Catalytic Activity

1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-{[(4-methoxybenzyl)(methyl)amino]methyl}-2-methylpropane-1,3-diol
cis-stilbene oxide + H2O = (1R,2R)-hydrobenzoin

Biophysicochemical Properties

0.52 uM for juvenile hormone III

Subunit

Homodimer.

Similarity

Belongs to the glutathione peroxidase family.
Belongs to the peptidase S33 family.

Keywords

3D-structure   Aromatic hydrocarbons catabolism   Complete proteome   Endoplasmic reticulum   Hydrolase   Membrane   Microsome   Reference proteome   Transmembrane   Transmembrane helix   Direct protein sequencing  

Feature

chain  Epoxide hydrolase

Uniprot

H9JWT0   C6KWM7   A0A0N0PD06   A0A2W1BCV7   A0A088M9L0   A0A2A4JKY8   + More
A0A3S2NYE4   A0A194PYB3   A0A1V0M8A5   A0A2H1VS44   A0A0P0ELB6   H9JWL6   Q6U6J0   A0A2W1B6Y8   O44124   Q1W696   Q94806   A0A0N0PEI4   A0A212F9W9   A0A3S2LEJ8   A0A2A4JV24   V5G602   D6WGJ2   D0W012   Q8MZR6   D0W015   A0A3S2NQU9   A0A0L7LT60   A0A2C9DK11   A0A2H1VAR3   A0A1W5KJY8   J3JY41   E9HGT3   A0A182ME36   A0A3S2P9A9   A0A0T6BDK1   R4G4K1   A0A1I8JUR7   A0A0F6SIA8   A0A3L8DLY6   A0A0N0P9P2   F4WV40   A0A0P5ZLI5   A0A1Y1K079   A0A1Y1K345   T1PCN6   A0A023ESJ9   C6KWM8   A0A0P5IQR9   A0A023EUU0   A0A2M4BMN3   A0A2M4BM54  

EC Number

3.3.2.9

Pubmed

19121390    20622465    26354079    28756777    26385554    28349297    + More
26445454    15681225    25143157    9927177    22118469    18362917    19820115    12125060    26227816    28536442    22516182    21292972    30249741    21719571    28004739    25315136    24945155   

EMBL

BABH01018608    AB293556    BAH97090.1    KQ460417    KPJ14867.1    KZ150476    + More
PZC70740.1    KJ579233    AIN34709.1    NWSH01001212    PCG72103.1    RSAL01000025    RVE52162.1    KQ459588    KPI97739.1    KU755500    ARD71210.1    ODYU01004100    SOQ43618.1    KT261710    ALJ30250.1    BABH01018379    AY377854    AB362775    AAQ87024.1    BAF81491.1    KZ150418    PZC70941.1    AF035482    AAB88192.1    DQ431847    ABD85119.1    U73680    AAB18243.1    KQ459764    KPJ20089.1    AGBW02009550    OWR50536.1    RSAL01000177    RVE45005.1    NWSH01000520    PCG75887.1    GALX01002976    JAB65490.1    KQ971321    EFA00568.1    AB534186    BAI49687.1    AF503908    AAM22694.1    AB534189    BAI49690.1    RSAL01000008    RVE53920.1    JTDY01000179    KOB78426.1    KX855995    ARE68677.1    ODYU01001536    SOQ37891.1    KU941586    AMR44689.1    BT128165    AEE63126.1    GL732643    EFX69015.1    AXCM01002766    RVE45007.1    LJIG01001532    KRT85408.1    ACPB03008739    GAHY01001290    JAA76220.1    KP271046    AKF11871.1    QOIP01000006    RLU21450.1    KQ459547    KPJ00030.1    GL888384    EGI61928.1    GDIP01250763    GDIP01043274    JAM60441.1    GEZM01096421    JAV54922.1    GEZM01096422    JAV54921.1    KA645900    AFP60529.1    GAPW01001360    JAC12238.1    AB293557    BAH97091.1    GDIQ01210360    GDIQ01190372    JAK41365.1    GAPW01001359    JAC12239.1    GGFJ01005082    MBW54223.1    GGFJ01005028    MBW54169.1   

Proteomes

UP000005204    UP000053240    UP000218220    UP000283053    UP000053268    UP000007151    + More
UP000007266    UP000037510    UP000000305    UP000075883    UP000015103    UP000075900    UP000279307    UP000007755    UP000095301   

PRIDE

H9JWT0    C6KWM7    H9JWL6    Q6U6J0    C6KWM8   

Pfam

PF06441   EHN        + More
PF00255   GSHPx
PF14529   Exo_endo_phos_2
PF00078   RVT_1

Interpro

IPR029759   GPX_AS        + More
IPR029760   GPX_CS       
IPR000889   Glutathione_peroxidase       
IPR029058   AB_hydrolase       
IPR036249   Thioredoxin-like_sf       
IPR010497   Epoxide_hydro_N       
IPR016292   Epoxide_hydrolase       
IPR000639   Epox_hydrolase-like       
IPR005135   Endo/exonuclease/phosphatase       
IPR036691   Endo/exonu/phosph_ase_sf       
IPR000477   RT_dom       

SUPFAM

SSF52833   SSF52833        + More
SSF53474   SSF53474       
SSF56219   SSF56219       

Gene 3D

3.60.10.10     3.40.50.1820    

CDD

cd00340  GSH_Peroxidase

ProteinModelPortal

H9JWT0   C6KWM7   A0A0N0PD06   A0A2W1BCV7   A0A088M9L0   A0A2A4JKY8   + More
A0A3S2NYE4   A0A194PYB3   A0A1V0M8A5   A0A2H1VS44   A0A0P0ELB6   H9JWL6   Q6U6J0   A0A2W1B6Y8   O44124   Q1W696   Q94806   A0A0N0PEI4   A0A212F9W9   A0A3S2LEJ8   A0A2A4JV24   V5G602   D6WGJ2   D0W012   Q8MZR6   D0W015   A0A3S2NQU9   A0A0L7LT60   A0A2C9DK11   A0A2H1VAR3   A0A1W5KJY8   J3JY41   E9HGT3   A0A182ME36   A0A3S2P9A9   A0A0T6BDK1   R4G4K1   A0A1I8JUR7   A0A0F6SIA8   A0A3L8DLY6   A0A0N0P9P2   F4WV40   A0A0P5ZLI5   A0A1Y1K079   A0A1Y1K345   T1PCN6   A0A023ESJ9   C6KWM8   A0A0P5IQR9   A0A023EUU0   A0A2M4BMN3   A0A2M4BM54  

PDB

4QLA     E-value=1.64658e-137,     Score=1256

KEGG

100307010    K10719 juvenile hormone epoxide hydrolase | (RefSeq) Jheh-lp3; juvenile hormone epoxide hydrolase-li

PATHWAY

00981    Insect hormone biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0016021   GO:0004602   GO:0006979   GO:0016787   GO:0016740   GO:0004601   GO:0033961   GO:0005789   GO:0019439   GO:0005737   GO:0006168   GO:0006261   GO:0001678   GO:0005536   GO:0046835   GO:0006281   GO:0006259   GO:0055114  

Subcellular location

Endoplasmic reticulum membrane  
Microsome membrane  

Length:

637

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

20.25292

Exp number, first 60 AAs:

20.1975

Total prob of N-in:

0.99548

POSSIBLE N-term signal

sequence

inside

1  -  12

TMhelix

13  -  32

outside

33  -  637

 

 

Pi

259.077384

Theta

168.849024

Tajima's D

1.604172

CLR

0.026608

CSRT

0.810559472026399

Interpretation

Uncertain