Gene

KWMTBOMO16183  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA013930

Annotation

juvenile_hormone_epoxide_hydrolase_[Bombyx_mori]

Location

Bomo_Chr28(-):733474-745866      View in EpiBrowser

Full name

Epoxide hydrolase       + More
Juvenile hormone epoxide hydrolase      
Glutathione peroxidase      
Juvenile hormone epoxide hydrolase 1      
Juvenile hormone epoxide hydrolase 2      

Alternative Name

CfEH1
Juvenile hormone epoxide hydrolase I
Juvenile hormone-specific epoxide hydrolase I
CfEH2
Juvenile hormone epoxide hydrolase II
Juvenile hormone-specific epoxide hydrolase II

Location in the cell

PlasmaMembrane   Reliability : 1.038

CDS

ATGTCACGTCTTCTTTTTATCGCCTTGCCGCTGTTAGTGCTGGCGTCCATACCATTGTACCTCCTCGTTCTGAAGAGTCCACCGCCGATGCCTAAGCTCGACCTGGAGGAGTGGTGGGGACCGCCCGAACTTAAGCAGAAACAGGATACCAGCATCAAACCCTTCGAGATCACTTTCAGTGAAACGATGGTAAAAGAACTTAAAGAACGCATCAAGAAACGAAGACCTTTCGCCCCTCCATTGGAGGGTGTTGGCTTCAAATACGGGTTCAATTCGAAACAATTGGACAGTTGGCTCAAATATTGGGCAGAAGAATACCCATTCGCTGAAAGGCAGAAGTTCCTTAACCAATATCCTCATTTCAAGACTAATATCCAGGGTTTGAATATTCACTTCATGAGGATTACTCCAAAGGTCCCTAAAGACGTTGAAATAGTACCACTTCTGTTACTCCACGGATGGCCGGGCTCCGTCAGGGAGTTCTACGAAGCCATTCCTCATCTCACAGCTGTCAGCAAAGACAGGAACTTCGCTCTGGAAATTATCGCTCCAAGTTTACCTGGTTATGGCTTTTCCGACGCGGCGGTTCGTCCCGGCCTCGCTGCTGCTGAAGTTGCAGTCATTTTCAAAAACCTGATGGCCAGACTTGGTTACAAGCAGTACTACGTCCAAGGAGGTGATTGGGGAGCTCTCATCGGTAGCGCTATGGCTACTTTCTTCCCTAAGGAAATAATCGGCTTCCACTCCAACATGGCGCTTACTCTTAGTCCAGCGGCGACATTCCTTGAGTTTGTCGGTGCCTTATTCCCATCTCTGATAGTCGAACCAGAACTAGCTAACAGACTTTACCCGTTATCGGAGAAATACTCAACCCTTCTCGAGGAATTGGGCTACATGCATATTCAAGCCACGAAGCCTGATACTGTTGGAATTGGATTAACAGACTCCCCGGCCGGTCTCCTCGCCTATATCCTGGAGAAGTTTTCCACTTGGACCAACCCCGACTTCCGCAGTAAAGAAGACGGCGGTCTCTCCTACCGGTGGACAAAGGACCAGCTGATCGATAATCTCATGCTGTACTGGAGCACGAAGTCAATAGTCACGTCAATGAGACTTTATGCTGAGTCTTTCAGTTCTAGGCACTTCGATTTAAAACTAGATGAGATTCAAGTACAAGTCCCAACGTGGGTGCTCCAGGCGAAATACGAGTTAGCCTACCAGCCACCCTGCATCCTCAAACTGAAGTACCCGAAACTGGTCAATGCCAGTGTCATTGAAGACGGAGGACATTTCCTTTCGTTTGAACTACCGGAGATCTTCGCCAAGGACGTCCTGAAGGCAATCGGCGAGTTCAGAAAACTCAAGAACGTGAAGACTGAGTTGTGA

Protein

MSRLLFIALPLLVLASIPLYLLVLKSPPPMPKLDLEEWWGPPELKQKQDTSIKPFEITFSETMVKELKERIKKRRPFAPPLEGVGFKYGFNSKQLDSWLKYWAEEYPFAERQKFLNQYPHFKTNIQGLNIHFMRITPKVPKDVEIVPLLLLHGWPGSVREFYEAIPHLTAVSKDRNFALEIIAPSLPGYGFSDAAVRPGLAAAEVAVIFKNLMARLGYKQYYVQGGDWGALIGSAMATFFPKEIIGFHSNMALTLSPAATFLEFVGALFPSLIVEPELANRLYPLSEKYSTLLEELGYMHIQATKPDTVGIGLTDSPAGLLAYILEKFSTWTNPDFRSKEDGGLSYRWTKDQLIDNLMLYWSTKSIVTSMRLYAESFSSRHFDLKLDEIQVQVPTWVLQAKYELAYQPPCILKLKYPKLVNASVIEDGGHFLSFELPEIFAKDVLKAIGEFRKLKNVKTEL

Description

Catalyzes juvenile hormone hydrolysis.
Catalyzes juvenile hormone hydrolysis. Degrades juvenile hormone III (JH III) about 3 times and 5 times slower than juvenile hormone I (JH I) and II (JH II), respectively. Degrades cis-stilbene oxide and trans-stilbene oxide about 18 and 43 times slower than JH III, respectively.

Catalytic Activity

1-(4-methoxyphenyl)-N-methyl-N-[(3-methyloxetan-3-yl)methyl]methanamine + H2O = 2-{[(4-methoxybenzyl)(methyl)amino]methyl}-2-methylpropane-1,3-diol
cis-stilbene oxide + H2O = (1R,2R)-hydrobenzoin

Biophysicochemical Properties

0.52 uM for juvenile hormone III

Subunit

Homodimer.

Similarity

Belongs to the peptidase S33 family.
Belongs to the glutathione peroxidase family.

Keywords

3D-structure   Aromatic hydrocarbons catabolism   Complete proteome   Endoplasmic reticulum   Hydrolase   Membrane   Microsome   Reference proteome   Transmembrane   Transmembrane helix   Direct protein sequencing  

Feature

chain  Epoxide hydrolase

Uniprot

H9JWL6   Q6U6J0   A0A2A4K8F5   A0A2W1B6Y8   C0KH33   O44124   + More
A0A194PZ44   A0A212F9W9   Q94806   A0A3S2LEJ8   A0A3S2P9A9   A0A194Q5D4   A0A0N0PEI4   Q1W696   L7R9X8   A0A2A4K9D8   A0A194PZ48   A0A2W1B2C5   A0A0N1IBG8   I4DKH4   D4Q9H4   A0A0N0PCA1   A0A194Q0T4   A0A194PZZ0   A0A0R6CEE9   A0A0N1IIM0   A0A194PZ55   C6KWM6   A0A212F9X4   H9JWT0   A0A2W1B693   A0A0N0PD06   C6KWM7   A0A3S2NYE4   H9JI63   A0A2W1BCV7   A0A0N1ICQ7   A0A2A4JI09   A0A088M9L0   A0A2A4JKY8   A0A2C9DK11   V5G602   A0A212F9Y7   A0A0T6BH94   A0A2A4J6Z6   A0A1Y1K079   A0A1I8JUR7   A0A1Y1K345   A0A0P0ELB6   D0W014   A0A1W4XE43   D6WGJ4   V5G055   D0W015   A0A2H1VS44   U5EUG0   A0A3G6VF49   A0A2D3FB68   Q7Q8D4   Q8MZR6   A0A2C9GS53   A0A1V0M8A5   A0A182U1G4   A0A182QWG9   A0A182USI7   D6WGJ2   Q16QD7   A0A182KYJ1   Q8MMJ6   A0A2J7PHG7   D0W012   A0A182ME36   Q86HE6   A0A0F6SIA8   A0A0N1IJV9   A0A0T6BDK1   A0A224XNL2   B3U0E6   A0A067QLM6   Q8MZR5   A0A2M4BM54   A0A2M4BMN3   A0A2M3ZA99   A0A154PRH0   J3JY41   A0A2M3Z944   W5JHA0   A0A0V0G516   A0A3S2NQU9   A0A2M4CM88   A0A2M4BMG5   A0A2A3E8M7   A0A2M4BM88   A0A336KD36   A0A2M4BM59   A0A151I9X6   A0A2M4BMA4   A0A0L7QRY7   A0A182G4D7   Q16QD6  

EC Number

3.3.2.9

Pubmed

19121390    15681225    25143157    28756777    26354079    22118469    + More
9927177    23276675    22651552    20622465    26385554    28536442    28004739    26445454    18362917    19820115    12364791    14747013    17210077    12125060    28349297    17510324    20966253    18579785    20075255    24845553    22516182    20920257    23761445    26483478   

EMBL

BABH01018379    AY377854    AB362775    AAQ87024.1    BAF81491.1    NWSH01000031    + More
PCG80507.1    KZ150418    PZC70941.1    FJ602793    ACM78602.2    AF035482    AAB88192.1    KQ459583    KPI98602.1    AGBW02009550    OWR50536.1    U73680    AAB18243.1    RSAL01000177    RVE45005.1    RVE45007.1    KPI98605.1    KQ459764    KPJ20089.1    DQ431847    ABD85119.1    JX681816    AGC00788.1    PCG80508.1    KPI98607.1    PZC70942.1    KQ459630    KPJ20526.1    AK401792    BAM18414.1    AB299279    BAI94514.1    KQ460733    KPJ12641.1    KPI98604.1    KPI98603.1    KM926340    AJR27470.1    KPJ20091.1    KPI98601.1    AB293555    BAH97089.1    OWR50537.1    BABH01018608    KZ150284    PZC70943.1    PZC71622.1    KQ460417    KPJ14867.1    AB293556    BAH97090.1    RSAL01000025    RVE52162.1    BABH01022402    KZ150476    PZC70740.1    KPJ20092.1    NWSH01001318    PCG71725.1    KJ579233    AIN34709.1    NWSH01001212    PCG72103.1    KX855995    ARE68677.1    GALX01002976    JAB65490.1    OWR50538.1    LJIG01000290    KRT86650.1    NWSH01002656    PCG67731.1    GEZM01096421    JAV54922.1    GEZM01096422    JAV54921.1    KT261710    ALJ30250.1    AB534188    BAI49689.1    KQ971321    EFA00569.1    GALX01005126    JAB63340.1    AB534189    BAI49690.1    ODYU01004100    SOQ43618.1    GANO01002338    JAB57533.1    MH932586    AZB52850.1    KY407548    ATU47254.1    AAAB01008944    EAA10122.2    AF503908    AAM22694.1    APCN01000682    KU755500    ARD71210.1    AXCN02002199    EFA00568.1    CH477751    EAT36615.1    AF517544    AAM88326.1    NEVH01025136    PNF15772.1    AB534186    BAI49687.1    AXCM01002766    AY212033    AAO52658.1    KP271046    AKF11871.1    KPJ20090.1    LJIG01001532    KRT85408.1    GFTR01006406    JAW10020.1    EU441215    ACC55233.1    KK853182    KDR10172.1    AF503909    AAM22695.1    GGFJ01005028    MBW54169.1    GGFJ01005082    MBW54223.1    GGFM01004705    MBW25456.1    KQ435007    KZC13710.1    BT128165    AEE63126.1    GGFM01004306    MBW25057.1    ADMH02001380    ETN62698.1    GECL01002991    JAP03133.1    RSAL01000008    RVE53920.1    GGFL01002133    MBW66311.1    GGFJ01005081    MBW54222.1    KZ288345    PBC27632.1    GGFJ01005024    MBW54165.1    UFQS01000285    UFQT01000285    SSX02441.1    SSX22816.1    GGFJ01005025    MBW54166.1    KQ978260    KYM95872.1    GGFJ01005078    MBW54219.1    KQ414766    KOC61382.1    JXUM01042080    KQ561310    KXJ79035.1    JF295126    ADZ76080.1    EAT36616.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000007151    UP000283053    UP000053240    + More
UP000075900    UP000192223    UP000007266    UP000007062    UP000075840    UP000075902    UP000075886    UP000075903    UP000008820    UP000075882    UP000235965    UP000075883    UP000002358    UP000027135    UP000076502    UP000000673    UP000242457    UP000078542    UP000053825    UP000069940    UP000249989   

PRIDE

H9JWL6    Q6U6J0    D4Q9H4    C6KWM6    H9JWT0    C6KWM7    + More
H9JI63   

Pfam

PF06441   EHN        + More
PF14529   Exo_endo_phos_2
PF00078   RVT_1
PF00255   GSHPx

Interpro

IPR029058   AB_hydrolase        + More
IPR016292   Epoxide_hydrolase       
IPR010497   Epoxide_hydro_N       
IPR000639   Epox_hydrolase-like       
IPR005135   Endo/exonuclease/phosphatase       
IPR036691   Endo/exonu/phosph_ase_sf       
IPR000477   RT_dom       
IPR029759   GPX_AS       
IPR029760   GPX_CS       
IPR000889   Glutathione_peroxidase       
IPR036249   Thioredoxin-like_sf       

SUPFAM

SSF53474   SSF53474        + More
SSF56219   SSF56219       
SSF52833   SSF52833       

Gene 3D

3.60.10.10     3.40.50.1820    

CDD

cd00340  GSH_Peroxidase

ProteinModelPortal

H9JWL6   Q6U6J0   A0A2A4K8F5   A0A2W1B6Y8   C0KH33   O44124   + More
A0A194PZ44   A0A212F9W9   Q94806   A0A3S2LEJ8   A0A3S2P9A9   A0A194Q5D4   A0A0N0PEI4   Q1W696   L7R9X8   A0A2A4K9D8   A0A194PZ48   A0A2W1B2C5   A0A0N1IBG8   I4DKH4   D4Q9H4   A0A0N0PCA1   A0A194Q0T4   A0A194PZZ0   A0A0R6CEE9   A0A0N1IIM0   A0A194PZ55   C6KWM6   A0A212F9X4   H9JWT0   A0A2W1B693   A0A0N0PD06   C6KWM7   A0A3S2NYE4   H9JI63   A0A2W1BCV7   A0A0N1ICQ7   A0A2A4JI09   A0A088M9L0   A0A2A4JKY8   A0A2C9DK11   V5G602   A0A212F9Y7   A0A0T6BH94   A0A2A4J6Z6   A0A1Y1K079   A0A1I8JUR7   A0A1Y1K345   A0A0P0ELB6   D0W014   A0A1W4XE43   D6WGJ4   V5G055   D0W015   A0A2H1VS44   U5EUG0   A0A3G6VF49   A0A2D3FB68   Q7Q8D4   Q8MZR6   A0A2C9GS53   A0A1V0M8A5   A0A182U1G4   A0A182QWG9   A0A182USI7   D6WGJ2   Q16QD7   A0A182KYJ1   Q8MMJ6   A0A2J7PHG7   D0W012   A0A182ME36   Q86HE6   A0A0F6SIA8   A0A0N1IJV9   A0A0T6BDK1   A0A224XNL2   B3U0E6   A0A067QLM6   Q8MZR5   A0A2M4BM54   A0A2M4BMN3   A0A2M3ZA99   A0A154PRH0   J3JY41   A0A2M3Z944   W5JHA0   A0A0V0G516   A0A3S2NQU9   A0A2M4CM88   A0A2M4BMG5   A0A2A3E8M7   A0A2M4BM88   A0A336KD36   A0A2M4BM59   A0A151I9X6   A0A2M4BMA4   A0A0L7QRY7   A0A182G4D7   Q16QD6  

PDB

4QLA     E-value=0,     Score=2236

KEGG

692686    K10719 juvenile hormone epoxide hydrolase | (RefSeq) Jheh2, JHEH, bommo-JHEH; juvenile hormone epoxi

PATHWAY

00981    Insect hormone biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0033961   GO:0005789   GO:0019439   GO:0016021   GO:0016787   GO:0004602   GO:0006979   GO:0004601   GO:0016740   GO:0003824   GO:0003676   GO:0016491   GO:0016311   GO:0016791   GO:0001678   GO:0005536   GO:0046835   GO:0006281   GO:0006259  

Subcellular location

Endoplasmic reticulum membrane  
Microsome membrane  

SignalP

Position:   1 - 25,         Likelihood:  0.597126

 

 

Length:

461

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

21.6503499999999

Exp number, first 60 AAs:

19.89314

Total prob of N-in:

0.93351

POSSIBLE N-term signal

sequence

inside

1  -  4

TMhelix

5  -  24

outside

25  -  461

 

 

Pi

226.313897

Theta

157.57552

Tajima's D

1.383895

CLR

0.383187

CSRT

0.760461976901155

Interpretation

Uncertain