SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO15964
Pre Gene Modal
BGIBMGA005722
Annotation
PREDICTED:_endothelin-converting_enzyme_1_[Bombyx_mori]
Full name
Neprilysin-3       + More
Endothelin-converting enzyme homolog      
Location in the cell
Cytoplasmic   Reliability : 1.346
 

Sequence

CDS
ATGCCAACTCTACATCTGAAACCCGGGATCACGATGGCTCGTTACAAGAAGACTGGTTTTATGGACGACGATGCCGGAAGCATCAGCTCGATCCAGCTCAACGAAGGAGTACGGTCCGGAGGAACTCATATTCGCTATCATATGGGTGTATCGTTCATAGGATGGCATTCGCGTACAAGATTGGAGCGTGTTCTGCTCGTGACCAGCGCTACGCTCTTTGTGGGTGTACTGCTCATGGTGACTCTTTTGCTAACCACAAGAGGTACTCCGGATATCCCTGTGCCGCCATGTTATCAGCCTGAACCTAAAGGCCCAAGCATTTGTCTTTCGAGCTCCTGCATTTACACTGCCAGCGAAGTTATCCAGGCCCTGGACGAAACTAAGGACCCGTGCGATGACTTCTACGAGTTCGCGTGTGGAGGATGGCTGAGGAACAATCCAATTCCTGAAGGAAAATCTACATGGGGTATCTTTAGTAAGATCGAGCTGCAGAACCAGTTGATCATCAGGTCCGCTCTGGAAAAGGTGAATACTACAAACAAATCGGAAGCAGAATATAAGGCGCGCGTGTATTATGATGCGTGCATCGACACGAATGAAACAATAGAGAAACTGGCGGAGAAACCTTTGATAGCCGTCATCAAGAAACTCGGAGGCTGGCATATACTGCCGTCAACGATGGTCAAACAGCAGAAATGGGACTTCCAGAAGCTGGTTCAGGACGTACAGAACTCATACAACCTGGGAGGCCTCTTCAACTGGGCCGTGTCTGAGGACGACAGAAACTCATCCAAACACGTCATCGTTCTTGACCAAGGAGGTCTTAATCTACCAACACGAGACAACTACCTGAACAAAACCGCCCATAAGAAGGTCCTAGACGCATATCTAGATTTTATGACTAGGATCTGTGTCCTCTTAGGCGCAAACAAATCCGAGGCTCGTGCCCAAATGAGCAAGGTCATTCAGTTCGAAACAGATTTAGCTAATATTACTACGCCCTCTGAAGACAGAAGAGATGAAGAAGGAATGTATAATCCTTATACAATAAGGCAGTGGCAGAAGGAAGTGTCTTTCTTGAATTGGACCCAGTTTTTTAATGATGCATTTAAGCTTGTCAATCGTACTATTCCAGATACGCAGAGAATTGTCGTTTACGCGCCAGACTACTTCAAGAATTTGACGAGGATCATACAAAAGTACAATAAAACTGAAGAAGACCAGAGAACGATAACCAGTTACATGATGTGGCAAGTTTCAAGATCTCTCTCCACTTATTTATCGAAGCCTTTTCGGGACGCCACCAAGATTCTTAGGAAGGCGCTTTTCGGATCTGAAGGCACCGAAGAGTCTTGGAGATACTGCGTCACTGACACTAACAATGCTATTGGTTTCGCGGTTGGAGCCATGTTCGTCCGCGAAGTCTTCCACGGCGAAGCGAAGACCCAGGGAGAAATAATGATTGATAACATCCGTAACGCTTTCAAGGAGAACCTGAGGAATTTGGACTGGATGGACACCGAGACGAGGAAAGCGGCCGAGACGAAGGCTGACGCTATAACTGATATGATCGGTTTCCCGGACTACATTCTCCACAAAGACGAACTGAACAAGCAATATGAAGAGTTGGTCGTTAAGCCAAACGAATACTTCGAAAATAACATAGCTTACAACATGTATTCGCTTAAGAACGACCTATTAAAACTGGATAAACCTGTCAACAAATCAAAATGGGGAATGACTCCGTCAACGGTAAACGCTTACTACACTCCCACGAAGAACCAAATAGTGTTTCCGGCCGGGATCCTGCAGTTGCCTTTCTATGATGGGGACAACCCGAAGTGCGTGAACTACGGGGCCATGGGGGTCGTCATGGGACACGAGCTGACCCACGCCTTTGACGATCAAGGAAGAGAATACGACAGATTTGGCAACTTGAACCAGTGGTGGAACGACGCGACGATTGCGAAATTCAAAAAACGCGCCGAATGCATTCGAGAACAATATTCGAAATATACAATGGACGGACATCATCTAAACGGGAAACAAACATTAGGGGAGAACATAGCCGACAACGGTGGCCTCAAAGCGTCATTCCACGCTTACCTGGACTACACCAAGAAAGCATCAGTTAACCTAACACTGCCGGGACTGAAATACAACGATAGACAACTTTTCTTTATTTCTTTTGCACAGGTATGGTGCTCGGCAATGACTAAAGAATCAACGAAAATGCAAATTGAGAAGGACGATCATACAGTTGCCAAATATCGAGTTATAGGACCAATAGCAAACCTAAAGGAGTTCTCCGACGAATTTCACTGTCCAATTGGTTCGAAAATGAATCCAACACATAAATGTGAAGTTTGGTAA
Protein
MPTLHLKPGITMARYKKTGFMDDDAGSISSIQLNEGVRSGGTHIRYHMGVSFIGWHSRTRLERVLLVTSATLFVGVLLMVTLLLTTRGTPDIPVPPCYQPEPKGPSICLSSSCIYTASEVIQALDETKDPCDDFYEFACGGWLRNNPIPEGKSTWGIFSKIELQNQLIIRSALEKVNTTNKSEAEYKARVYYDACIDTNETIEKLAEKPLIAVIKKLGGWHILPSTMVKQQKWDFQKLVQDVQNSYNLGGLFNWAVSEDDRNSSKHVIVLDQGGLNLPTRDNYLNKTAHKKVLDAYLDFMTRICVLLGANKSEARAQMSKVIQFETDLANITTPSEDRRDEEGMYNPYTIRQWQKEVSFLNWTQFFNDAFKLVNRTIPDTQRIVVYAPDYFKNLTRIIQKYNKTEEDQRTITSYMMWQVSRSLSTYLSKPFRDATKILRKALFGSEGTEESWRYCVTDTNNAIGFAVGAMFVREVFHGEAKTQGEIMIDNIRNAFKENLRNLDWMDTETRKAAETKADAITDMIGFPDYILHKDELNKQYEELVVKPNEYFENNIAYNMYSLKNDLLKLDKPVNKSKWGMTPSTVNAYYTPTKNQIVFPAGILQLPFYDGDNPKCVNYGAMGVVMGHELTHAFDDQGREYDRFGNLNQWWNDATIAKFKKRAECIREQYSKYTMDGHHLNGKQTLGENIADNGGLKASFHAYLDYTKKASVNLTLPGLKYNDRQLFFISFAQVWCSAMTKESTKMQIEKDDHTVAKYRVIGPIANLKEFSDEFHCPIGSKMNPTHKCEVW

Summary

Description
Metalloendoprotease which is required in the dorsal paired medial neurons for the proper formation of long-term (LTM) and middle-term memories (MTM). Also required in the mushroom body neurons where it functions redundantly with neprilysins Nep2 and Nep4 in normal LTM formation.
Catalytic Activity
Preferential cleavage of polypeptides between hydrophobic residues, particularly with Phe or Tyr at P1'.
Cofactor
Zn(2+)
Similarity
Belongs to the peptidase M13 family.
Keywords
Cell membrane   Complete proteome   Disulfide bond   Glycoprotein   Hydrolase   Membrane   Metal-binding   Metalloprotease   Protease   Reference proteome   Signal-anchor   Transmembrane   Transmembrane helix   Zinc  
Feature
chain  Neprilysin-3
EC Number
3.4.24.11
3.4.24.-
EMBL
BABH01038191    BABH01038192    BABH01038193    RSAL01000012    RVE53513.1    KQ459590    + More
KPI97453.1    NWSH01000851    PCG73849.1    ODYU01010405    SOQ55484.1    AGBW02014530    OWR41506.1    AXCN02002075    AAAB01008964    EAA12912.1    APCN01005203    AXCM01004319    ATLV01013267    KE524849    KFB37623.1    NEVH01005280    PNF39029.1    GBYB01014696    JAG84463.1    GFDL01000411    JAV34634.1    GFDL01000406    JAV34639.1    GBYB01014697    JAG84464.1    GFDL01000385    JAV34660.1    GFDL01000415    JAV34630.1    GFDL01000426    JAV34619.1    KK852548    KDR21598.1    NNAY01003046    OXU20060.1    GAMC01010552    JAB96003.1    CH477758    EAT36553.1    GAMC01010551    JAB96004.1    ADMH02002119    ETN58796.1    QOIP01000001    RLU26255.1    GGFL01000152    MBW64330.1    KK107218    EZA55169.1    GGFL01000151    MBW64329.1    GGFK01003057    MBW36378.1    GEDC01022659    GEDC01003191    JAS14639.1    JAS34107.1    GECU01009716    JAS97990.1    GFDL01000413    JAV34632.1    CH902621    EDV44632.1    CM000162    EDX02067.2    KRK06544.1    KPU81656.1    CCAG010015359    KPU81655.1    CH954180    EDV46768.1    AE014298    AY095202    BT132915    AAM12295.1    AEW12887.1    JXJN01003092    JXJN01003093    KQS30114.1    GFDL01000418    JAV34627.1    AY149919    AAN73018.1    GL434809    EFN74503.1    GGFJ01002285    MBW51426.1    GGFJ01002275    MBW51416.1    CH379064    KRT06767.1    KZ288204    PBC33201.1    EAL32008.2    GBBI01002796    JAC15916.1    GL445407    EFN89640.1    KRT06765.1    KRT06766.1    OUUW01000003    SPP78516.1    CH963925    EDW78269.2    KRF98844.1    JXUM01056431    KQ561910    KXJ77198.1    CH916371    EDV91912.1    KQ434931    KZC11845.1    KQ414615    KOC68757.1    GDKW01000749    JAI55846.1    KQ760382    OAD60700.1    CH940651    EDW65857.1    KRF82444.1    CP012528    ALC49112.1    GGFM01002934    MBW23685.1    KQ435714    KOX79211.1    UFQS01000201    UFQT01000201    SSX01253.1    SSX21633.1    GEZM01096133    JAV55135.1    KQ971345    EFA05107.2    AJWK01011058   
Pfam
PF05649   Peptidase_M13_N        + More
PF01431   Peptidase_M13
Interpro
IPR042089   Peptidase_M13_dom_2        + More
IPR008753   Peptidase_M13_N       
IPR024079   MetalloPept_cat_dom_sf       
IPR018497   Peptidase_M13_C       
IPR000718   Peptidase_M13       
IPR029734   ECE1       
Gene 3D
PDB
3DWB     E-value=0,     Score=1653

Ontologies

Topology

Subcellular location
Cell membrane  
Length:
790
Number of predicted TMHs:
1
Exp number of AAs in TMHs:
21.53308
Exp number, first 60 AAs:
0.06227
Total prob of N-in:
0.96363
inside
1  -  63
TMhelix
64  -  86
outside
87  -  790
 
 

Population Genetic Test Statistics

Pi
185.23701
Theta
157.846155
Tajima's D
0.321105
CLR
0.852207
CSRT
0.460626968651567
Interpretation
Uncertain
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