Gene

KWMTBOMO15768  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002237

Annotation

PREDICTED:_serine/threonine-protein_phosphatase_PP2A_65_kDa_regulatory_subunit_[Papilio_polytes]

Location

Bomo_Chr26(-):9607275-9616187      View in EpiBrowser

Full name

Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform      

Alternative Name

PP2A subunit A isoform PR65-alpha
PP2A subunit A isoform R1-alpha

Location in the cell

Cytoplasmic   Reliability : 1.788

CDS

ATGACTTGTTTTTACGATGAGCTAAAGAATGAGGATGTACAGCTTCGTTTGAACTCTATAAAGAAATTGTCGACGATCGCGTTGGCTCTGGGCGTGGAGAGGACTAAATCGGAGTTGATCCCGTTCCTCACTGAGACAATCTATGATGAAGACGAGGTTCTCTTAGCACTCGCTGAACAACTTGGTAACTTCATAAACTTGGTTGGAGGGGGAGAATTTGCACACTGTCTCCTTCCTCCACTAGAGACATTAGCAGCAGTGGAAGAAACAGTAGTCAGAGACAAGGCAGTGGCCTCGCTCCGTGCTGTTGCCGAGCACCATAGCCCCCAGGCACTCGAGGAACACTTTGTGCCATTAGTACAGCGTCTTGCCGGAGGAGACTGGTTTACTTCCAGAACATCTGCCTGTGGACTCTTTAGTGTCTGCTACCCACGCGTTTCGGCCGTAGTGAAGGCGGAACTTCGTCAGCATTTTTGCTCGCTCTGCCAAGATGACACTCCGATGGTGCGACGCGCTGCCGCCTACAAACTTGGGGAGTTCGCTAAAGTCGTAGAAATCGAATACGTCAAGAGCGATCTCATTCCCATCTTTGTGTTTTTGGCAAAGGATGACCAAGACTCGGTACGTTTGCTAGCCGCAGAGGCATGTGCTGTTGTGGCTTCTCTGCTGGCACCCGAGGATATGGAGCAACATGTGATGCCGACCGTGCGGGCTCGTGCCGGAGATACTTCGTGGAGGGTGCGGTACATGGTCGCTGATAAGTTCGTAGAGCTGCAACAAGCTGTTGGTCCAGAGCTAGCTCGTACAGATCTAGCACAGATCTTCCAGGCACTGCTCAAGGACTCGGAGGCTGAGGTTAGAGCCGCAGCTGCTGGGAAGGTCAAGGACTTCTGTATGAACTTGGATAAAGCTCATCAAGAACACATCATCATGACCATGATCTTGCCACAGATCAAGGACTTGGTGTGTGATGCCAACCAACACGTCAAGTCTGCCCTAGCCTCCGTCATCATGGGCCTGAGCCCCATAGTGGGCAGACAGAACACTATTGAACATCTCCTGCCGCTGTTCCTCACACAACTGAAGGATGAATGTCCCGAAGTCCGACTCAACATAATCTCCAACCTCGAGTGTGTGAATGAAGTGATCGGCATCCAACAGCTGGTGCAGTCTCTCCTCCCCGCCATCGTGGAGCTGGCAGAGGACACCAAGTGGAGGGTCCGTCTGGCCATCATCGAGCACATGCCGCTACTGGCGGGACAGCTGGGCCAGGAGTTCTTCGACGAGAAGCTGACCAGCTTGTGCATGTCGTGGCTGGTGGACCACGTGTACGCCATCCGCGAGGCTGCGACCCTCAACCTGAAGAAGCTGGTGGAGCAGTACGGGCCGCAGTGGGCAGAGAACAACGTCATACCCAAAGTACTGAACATGTCGCACGAGCAGAACTACTTGCACAGGATGACGTACTTGTTCTGCATTAACGTGCTGTCCGAAGTGTGCGGCAAGGACATCACGACCAGGGTGCTGCTGCCGACCGTGTTGTCTATGGCCGACGACAATGTTGCCAACGTGCGGTTCAATGTTGCCAAGACGCTGCAGATAATGGCCAAATACTTGGACCCGGCCGTCATCCAGCCCCAAGTCAAGCCGGTCCTGGAGAAGCTGAACGTGGACCCCGACGTCGACGTCAAGTACTTCGCATCGGAAGCGATCGCCGGAATAGCTGGATAA

Protein

MTCFYDELKNEDVQLRLNSIKKLSTIALALGVERTKSELIPFLTETIYDEDEVLLALAEQLGNFINLVGGGEFAHCLLPPLETLAAVEETVVRDKAVASLRAVAEHHSPQALEEHFVPLVQRLAGGDWFTSRTSACGLFSVCYPRVSAVVKAELRQHFCSLCQDDTPMVRRAAAYKLGEFAKVVEIEYVKSDLIPIFVFLAKDDQDSVRLLAAEACAVVASLLAPEDMEQHVMPTVRARAGDTSWRVRYMVADKFVELQQAVGPELARTDLAQIFQALLKDSEAEVRAAAAGKVKDFCMNLDKAHQEHIIMTMILPQIKDLVCDANQHVKSALASVIMGLSPIVGRQNTIEHLLPLFLTQLKDECPEVRLNIISNLECVNEVIGIQQLVQSLLPAIVELAEDTKWRVRLAIIEHMPLLAGQLGQEFFDEKLTSLCMSWLVDHVYAIREAATLNLKKLVEQYGPQWAENNVIPKVLNMSHEQNYLHRMTYLFCINVLSEVCGKDITTRVLLPTVLSMADDNVANVRFNVAKTLQIMAKYLDPAVIQPQVKPVLEKLNVDPDVDVKYFASEAIAGIAG

Description

The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit. Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT. Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis.
The PR65 subunit of protein phosphatase 2A serves as a scaffolding molecule to coordinate the assembly of the catalytic subunit and a variable regulatory B subunit (PubMed:10100624). Upon interaction with GNA12 promotes dephosphorylation of microtubule associated protein TAU/MAPT (By similarity). Required for proper chromosome segregation and for centromeric localization of SGO1 in mitosis (By similarity).

Subunit

PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9. Interacts with TP53 and SGO1. Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD. Interacts with PLA2G16; this interaction might decrease PP2A activity. Interacts with FOXO1; the interaction dephosphorylates FOXO1 on AKT-mediated phosphorylation sites. Interacts with CTTNBP2NL. Interacts with GNA12; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT. Interacts with CIP2A; this interaction stabilizes CIP2A (By similarity). Interacts with FAM122A (By similarity). Interacts with ADCY8; antagonizes interaction between ADCY8 and calmodulin (By similarity). Interacts with CRTC3 (when phosphorylated at 'Ser-391') (By similarity).
PP2A consists of a common heterodimeric core enzyme, composed of PPP2CA a 36 kDa catalytic subunit (subunit C) and PPP2R1A a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules. Interacts with IPO9 (By similarity). Interacts with TP53 and SGO1 (By similarity). Found in a complex with at least ARL2, PPP2CB, PPP2R1A, PPP2R2A, PPP2R5E and TBCD (By similarity). Interacts with PLA2G16; this interaction might decrease PP2A activity (By similarity). Interacts with FOXO1; the interaction dephosphorylates FOXO1 on AKT-mediated phosphorylation sites (PubMed:22417654). Interacts with CTTNBP2NL (By similarity). Interacts with GNA12; the interaction promotes protein phosphatase 2A activation causing dephosphorylation of MAPT (By similarity).Interacts with CIP2A; this interaction stabilizes CIP2A (By similarity). Interacts with FAM122A (By similarity). Interacts with ADCY8; antagonizes interaction between ADCY8 and calmodulin. Interacts with CRTC3 (when phosphorylated at 'Ser-391') (PubMed:30611118).

Similarity

Belongs to the phosphatase 2A regulatory subunit A family.

Keywords

Acetylation   Cell membrane   Cell projection   Centromere   Chromosome   Chromosome partition   Complete proteome   Cytoplasm   Membrane   Nucleus   Reference proteome   Repeat   3D-structure  

Feature

chain  Serine/threonine-protein phosphatase 2A 65 kDa regulatory subunit A alpha isoform

Uniprot

H9IYA4   H9IYK6   F5C3T7   A0A2H1X028   A0A194QF74   A0A0N0PAZ1   + More
S4PGS2   A0A1E1W1R9   F4WYE4   A0A158NXP8   A0A151WPX9   E9J0Q7   A0A0J7KU48   E1ZYP9   A0A195EV27   A0A1W4X0F5   A0A1W4X9W6   Q5F303   A0A1Y1MEW1   D6X2U1   A0A1Y1MK82   A0A026WJR2   E2BS64   A0A2J7PMR1   A0A067RRK4   A0A2J7PMP8   J3JZB5   K7IW91   A0A232EQS9   N6TCF6   A0A087ZQA3   A0A2A3EG59   A0A1S4EJQ5   A0A1B6FR68   A0A1B6KRX0   A0A1B6EHF0   A0A1B6M1B4   T1J3L9   A0A1B6C9G4   A0A1B6CBY7   A0A1B6DBN8   A0A1W7R9K6   A0A0P4VJ81   A0A0V0G412   A0A069DVN3   T1H983   A0A2R5LEA6   A0A131Y1Y5   A0A224XGT3   V5HVQ0   A0A0L7QUV4   U4UVC1   A0A293MSE6   A0A1Z5KVU6   A0A0B8RP59   B7PA24   A0A1W7RDE6   T1E4V0   A0A0F7Z5F7   U3FB63   H9GGK1   A0A0N0BHJ3   A0A2Y9DWC4   A0A2Y9LE43   M3Z137   A0A3Q7MDD0   A0A2I2V4A6   A0A3Q7V4T8   A0A0A9Z6Z2   Q7ZWS7   A0A3Q1LW84   A0A2U3ZW71   A0A3Q7VMD3   A0A0P6JF70   A0A3L7IA09   A0A1U7R3I2   Q5XI34   G1LUS6   F1PX75   A0A341DBD8   A0A340X9T8   A0A2Y9EQM8   A0A2Y9GDL4   M3W170   A0A2Y9L1P1   A0A3Q7TJL4   A0A2Y9P9C0   A0A1U7TCT8   A0A2K6EPM9   A0A3Q7V4U4   A0A3Q7MED3   P54612   Q76MZ3   S4RKW9   A0A250YKC1   G1NY18   D2HXP5   A0A3Q7U6Z0   A0A2K6CBT5   A0A2K6KFG8  

Pubmed

19121390    23557680    26354079    23622113    21719571    21347285    + More
21282665    20798317    28004739    18362917    19820115    24508170    24845553    22516182    20075255    28648823    23537049    27129103    26334808    25765539    28528879    25476704    26358130    23758969    23915248    21881562    17975172    25401762    26823975    27762356    19393038    29704459    15489334    15057822    15632090    20010809    16341006    10100624    16141072    16258073    21183079    22417654    23806337    30611118    28087693    21993624   

EMBL

BABH01020881    JF460008    AEB77782.1    ODYU01012348    SOQ58617.1    KQ459144    + More
KPJ03595.1    KQ461111    KPJ08931.1    GAIX01003527    JAA89033.1    GDQN01010116    JAT80938.1    GL888440    EGI60796.1    ADTU01003277    KQ982851    KYQ49861.1    GL767538    EFZ13522.1    LBMM01003176    KMQ93848.1    GL435242    EFN73589.1    KQ981958    KYN32006.1    AJ876407    CAI45288.1    GEZM01033297    JAV84274.1    KQ971372    EFA10293.2    GEZM01033296    JAV84276.1    KK107168    EZA56198.1    GL450151    EFN81456.1    NEVH01023960    PNF17616.1    KK852469    KDR23265.1    PNF17614.1    BT128596    AEE63553.1    NNAY01002716    OXU20710.1    APGK01043334    APGK01043335    APGK01043336    APGK01043337    KB741014    ENN75423.1    KZ288254    PBC30727.1    GECZ01017099    JAS52670.1    GEBQ01025778    JAT14199.1    GECZ01032455    JAS37314.1    GEBQ01010297    JAT29680.1    JH431829    GEDC01027176    GEDC01019288    GEDC01011774    GEDC01010575    GEDC01002434    JAS10122.1    JAS18010.1    JAS25524.1    JAS26723.1    JAS34864.1    GEDC01026331    JAS10967.1    GEDC01014195    JAS23103.1    GFAH01000563    JAV47826.1    GDKW01001581    JAI55014.1    GECL01003343    JAP02781.1    GBGD01000924    JAC87965.1    ACPB03022440    ACPB03022441    GGLE01003561    MBY07687.1    GEFM01002899    JAP72897.1    GFTR01007398    JAW09028.1    GANP01004121    JAB80347.1    KQ414731    KOC62413.1    KB632381    ERL94150.1    GFWV01019085    MAA43813.1    GFJQ02007956    JAV99013.1    GBSH01002502    JAG66524.1    ABJB010192854    ABJB010726455    ABJB010801276    DS668644    EEC03446.1    GDAY02002263    JAV49169.1    GAAZ01002306    JAA95637.1    GBEX01003137    JAI11423.1    GAEP01001497    GBEW01000945    JAB53324.1    JAI09420.1    AAWZ02023228    KQ435750    KOX76312.1    AEYP01098490    AANG04000747    GBHO01006029    GDHC01020677    JAG37575.1    JAP97951.1    BC046723    CM004478    AAH46723.1    OCT73313.1    GEBF01000500    JAO03133.1    RAZU01000084    RLQ74810.1    AABR07001873    BC083859    CH474033    AAH83859.1    EDL99781.1    ACTA01138807    AAEX03000754    Z34955    AB021743    AK054239    AK078135    BC006606    GFFW01000822    JAV43966.1    AAPE02043049    AAPE02043050    AAPE02043051    GL193637    EFB14807.1   

Proteomes

UP000005204    UP000053268    UP000053240    UP000007755    UP000005205    UP000075809    + More
UP000036403    UP000000311    UP000078541    UP000192223    UP000007266    UP000053097    UP000008237    UP000235965    UP000027135    UP000002358    UP000215335    UP000019118    UP000005203    UP000242457    UP000079169    UP000015103    UP000053825    UP000030742    UP000001555    UP000001646    UP000053105    UP000248480    UP000248482    UP000000715    UP000286641    UP000011712    UP000286642    UP000186698    UP000009136    UP000245340    UP000273346    UP000189706    UP000002494    UP000008912    UP000002254    UP000252040    UP000265300    UP000248484    UP000248481    UP000286640    UP000248483    UP000189704    UP000233160    UP000008227    UP000000589    UP000245300    UP000001074    UP000233120    UP000233180   

PRIDE

H9IYA4    H9IYK6    V5HVQ0    B7PA24    F1PX75    P54612    + More
Q76MZ3   

Pfam

PF02985   HEAT        + More
PF00226   DnaJ

Interpro

IPR021133   HEAT_type_2        + More
IPR016024   ARM-type_fold       
IPR011989   ARM-like       
IPR000357   HEAT       
IPR034085   TOG       
IPR001623   DnaJ_domain       
IPR036869   J_dom_sf       
IPR031090   PP2A_A_meta       

SUPFAM

SSF48371   SSF48371        + More
SSF46565   SSF46565       

Gene 3D

1.25.10.10     1.10.287.110    

CDD

cd06257  DnaJ

ProteinModelPortal

H9IYA4   H9IYK6   F5C3T7   A0A2H1X028   A0A194QF74   A0A0N0PAZ1   + More
S4PGS2   A0A1E1W1R9   F4WYE4   A0A158NXP8   A0A151WPX9   E9J0Q7   A0A0J7KU48   E1ZYP9   A0A195EV27   A0A1W4X0F5   A0A1W4X9W6   Q5F303   A0A1Y1MEW1   D6X2U1   A0A1Y1MK82   A0A026WJR2   E2BS64   A0A2J7PMR1   A0A067RRK4   A0A2J7PMP8   J3JZB5   K7IW91   A0A232EQS9   N6TCF6   A0A087ZQA3   A0A2A3EG59   A0A1S4EJQ5   A0A1B6FR68   A0A1B6KRX0   A0A1B6EHF0   A0A1B6M1B4   T1J3L9   A0A1B6C9G4   A0A1B6CBY7   A0A1B6DBN8   A0A1W7R9K6   A0A0P4VJ81   A0A0V0G412   A0A069DVN3   T1H983   A0A2R5LEA6   A0A131Y1Y5   A0A224XGT3   V5HVQ0   A0A0L7QUV4   U4UVC1   A0A293MSE6   A0A1Z5KVU6   A0A0B8RP59   B7PA24   A0A1W7RDE6   T1E4V0   A0A0F7Z5F7   U3FB63   H9GGK1   A0A0N0BHJ3   A0A2Y9DWC4   A0A2Y9LE43   M3Z137   A0A3Q7MDD0   A0A2I2V4A6   A0A3Q7V4T8   A0A0A9Z6Z2   Q7ZWS7   A0A3Q1LW84   A0A2U3ZW71   A0A3Q7VMD3   A0A0P6JF70   A0A3L7IA09   A0A1U7R3I2   Q5XI34   G1LUS6   F1PX75   A0A341DBD8   A0A340X9T8   A0A2Y9EQM8   A0A2Y9GDL4   M3W170   A0A2Y9L1P1   A0A3Q7TJL4   A0A2Y9P9C0   A0A1U7TCT8   A0A2K6EPM9   A0A3Q7V4U4   A0A3Q7MED3   P54612   Q76MZ3   S4RKW9   A0A250YKC1   G1NY18   D2HXP5   A0A3Q7U6Z0   A0A2K6CBT5   A0A2K6KFG8  

PDB

2PF4     E-value=0,     Score=2203

KEGG

101741612    K03456 serine/threonine-protein phosphatase 2A regulatory subunit A | (RefSeq) serine/threonine-prot

PATHWAY

03015    mRNA surveillance pathway - Bombyx mori (domestic silkworm)
04350    TGF-beta signaling pathway - Bombyx mori (domestic silkworm)
04391    Hippo signaling pathway - fly - Bombyx mori (domestic silkworm)

GO

GO:0000159   GO:0019888   GO:0006470   GO:0005737   GO:0065003   GO:0046982   GO:0000775   GO:0005634   GO:1990405   GO:0007059   GO:0051754   GO:2001241   GO:0051232   GO:0098978   GO:0005829   GO:1903538   GO:0004722   GO:0070262   GO:0051306   GO:0007143   GO:0030425   GO:0016328   GO:0045202   GO:0005515   GO:0005488   GO:0006468   GO:0008270   GO:0005215   GO:0006270   GO:0042555   GO:0003707   GO:0006281   GO:0006259  

PANTHER

PTHR10648:SF2  

Subcellular location

Cytoplasm   Centromeric localization requires the presence of BUB1.   With evidence from 1 publications.
Nucleus   Centromeric localization requires the presence of BUB1.   With evidence from 1 publications.
Chromosome   Centromeric localization requires the presence of BUB1.   With evidence from 1 publications.
Centromere   Centromeric localization requires the presence of BUB1.   With evidence from 1 publications.
Lateral cell membrane   Centromeric localization requires the presence of BUB1.   With evidence from 1 publications.
Cell projection   Centromeric localization requires the presence of BUB1.   With evidence from 1 publications.
Dendrite   Centromeric localization requires the presence of BUB1.   With evidence from 1 publications.

Length:

576

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.01493

Exp number, first 60 AAs:

0.00347

Total prob of N-in:

0.00186

outside

1  -  576

 

 

Pi

227.104548

Theta

197.91259

Tajima's D

0.350727

CLR

0

CSRT

0.469926503674816

Interpretation

Uncertain