Gene

KWMTBOMO15729  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002318

Annotation

high_temperature_requirement_protein_A2_[Bombyx_mori]

Location

Bomo_Chr26(+):7677377-7687091      View in EpiBrowser

Full name

Serine protease HTRA2, mitochondrial      

Alternative Name

High temperature requirement protein A2
Omi stress-regulated endoprotease

Location in the cell

Mitochondrial   Reliability : 1.531 PlasmaMembrane   Reliability : 1.011

CDS

ATGAATTTAAGGCTTTTCCGTATTTTTAAAACAAATACTATATCCAAAATAAACTACAGATGTACTAGTCGCGTTGTGCCAATACTAAATAACTACAAAAGTAACAGTGATGACAATCAAAGCTCTTACTTTAGATGCATTTTAGGAGCCACTATAGGGTTTATAGGTTATTTTAGTTTACGTGAAAAGGTAACAGCTGCTACAGTTGTAAATGATTTGAAAGGACGAAGGGAGAAATATAATTTTATAGCTGACGTTGTGGCAGTTTCGGCGGCGTCTGTTGTGTATATAGAAATAGTAGACGGACGAAGAATAGATGCGTTTACCGGTAAAAAATTAAAGATATCCAATGGCTCGGGATTCATCATTAAGGAAGATGGGCTGATATTAACTAACGCTCATGTTGTAGTCAATAAGCCTAATGCAATCGTCAAAGTTAGATTAACTGATGGTTCAACACACGAGGCCCTCATAGAACACTATGACCTACAATCAGATCTAGCAACACTTCGGATACCAGTCAAAGGACTACCAACAATGAAACTTGGTACTTCCGCTGATCTCAAGCCTGGCGAGTGGGTGGTGGCTATAGGAAGTCCCCTTGATTTGAGCAACACAGTCACAGCTGGTGTGGTGAGCTCTACTCAGAGAGCTGGAAGCGAACTTGGCTTACAAGACAGAAATATAGTATACATACAAACAGATGCTCCGATTACATTCGGTAACAGCGGGGGACCTCTTGTCAATCTCGACGGCGAAGCTATAGGCATTAACAGCATGAAAGTCACCTACGGAATATCGTTTGCCATACCCATAGATTATGTAAAGGAATTCTTGGCTAAACATAAAACCAAGTCTCCTCAAGTCTCGAAGCGTTACCTTGGCATCACCATGCTCTCATTAACGCCTTCCATACTCATGGAGTTGAAGATGAGAAATCCCGAGATGCCAACCGATATTCAACACGGCATCCTTGTATGGAAGGTCATCATTGGATCGCCGGCTTTCAACGGGGGTCTACAACCAGGCGACATTGTAGTCAAAATAAACGGAAAACCAGTTCACAATACAACCGATATATACAACATTCTAGAAAGTACGACGGGGAGTTTGAAGATTGACGCCGTCAGAGGGCGCCAGCAGATAAACCTGACCATAGTACCAGAGTTACACTGA

Protein

MNLRLFRIFKTNTISKINYRCTSRVVPILNNYKSNSDDNQSSYFRCILGATIGFIGYFSLREKVTAATVVNDLKGRREKYNFIADVVAVSAASVVYIEIVDGRRIDAFTGKKLKISNGSGFIIKEDGLILTNAHVVVNKPNAIVKVRLTDGSTHEALIEHYDLQSDLATLRIPVKGLPTMKLGTSADLKPGEWVVAIGSPLDLSNTVTAGVVSSTQRAGSELGLQDRNIVYIQTDAPITFGNSGGPLVNLDGEAIGINSMKVTYGISFAIPIDYVKEFLAKHKTKSPQVSKRYLGITMLSLTPSILMELKMRNPEMPTDIQHGILVWKVIIGSPAFNGGLQPGDIVVKINGKPVHNTTDIYNILESTTGSLKIDAVRGRQQINLTIVPELH

Description

Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Can antagonize antiapoptotic activity of th by directly inducing the degradation of th (By similarity).
Serine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Can antagonize antiapoptotic activity of th by directly inducing the degradation of th.

Catalytic Activity

Cleavage of non-polar aliphatic amino-acids at the P1 position, with a preference for Val, Ile and Met. At the P2 and P3 positions, Arg is selected most strongly with a secondary preference for other hydrophilic residues.

Subunit

Interacts with th/DIAP1 (via BIR 2 domain).

Similarity

Belongs to the peptidase S1C family.

Keywords

Apoptosis   Complete proteome   Hydrolase   Membrane   Mitochondrion   Protease   Reference proteome   Serine protease   Transit peptide   Transmembrane   Transmembrane helix   Zymogen   Direct protein sequencing   Ubl conjugation  

Feature

chain  Serine protease HTRA2, mitochondrial

Uniprot

E9JEH9   A0A2A4JQZ8   A0A2W1BPY2   A0A2A4JQ82   A0A194QNW3   A0A2H1WSF0   + More
A0A0N1IGF8   B4G316   Q297U2   A0A0M5J9A9   D6WGF2   A0A1W4USN6   A0A0B4KG52   Q9VFJ3   B4HEM8   B4K835   B4QZU6   B4LY58   B3LVG7   B4PST0   B3P3J9   Q176F8   A0A023ET05   A0A3B0JDW7   Q176F7   A0A1S4FDA2   A0A336L1Z8   A0A1Y1KS05   A0A0K8VBF4   W8C2G7   A0A0A1WGS0   A0A1Q3F965   A0A182H2K1   B4N937   A0A336M9M5   A0A034W811   A0A0K8TK95   A0A0K8VAR6   A0A1Q3FBA9   B4JTT7   A0A1A9UEQ4   A0A1A9X9Z1   A0A1B0BH89   A0A1B0G0K2   A0A1A9ZMD8   A0A084VLM0   A0A1A9WNT6   A0A1I8PU85   A0A182KS70   Q7QF03   A0A182XPG7   A0A182VJQ7   A0A1I8M2X9   A0A182UBC6   A0A182IAG4   A0A0L0CA47   A0A1J1HLP9   A0A2J7RFQ5   K7IR33   A0A232EY33   A0A182M124   A0A154PIY5   A0A1B0D3U5   A0A1L8DTK2   A0A1B0CSJ1   A0A182IKI5   A0A182N0B5   A0A182QEE8   A0A182XY18   A0A1V1FKJ0   A0A182RQ72   A0A182T6P8   N6T759   A0A182W597   A0A067RIC9   A0A182K9C3   A0A2M4BRC5   A0A2M4BR44   A0A2M4BR51   A0A2M3ZAZ8   W5JAK0   E2AUP0   A0A2M4ALR3   A0A2M4ANZ4   E2BYN0   A0A151IDG5   A0A182FJ30   A0A151WEF3   T1HZX5   A0A224XFH9   E9IAJ5   A0A195EE27   A0A195FFQ2   A0A182PR27   A0A195BEW1   A0A158NUD5   T1IQJ2   B0WE06   A0A068F678   F4X495  

EC Number

3.4.21.108

Pubmed

19121390    21040523    28756777    26354079    17994087    15632085    + More
18362917    19820115    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    17397804    17557079    18259196    12537569    17510324    24945155    28004739    24495485    25830018    26483478    25348373    26369729    24438588    20966253    12364791    14747013    17210077    25315136    26108605    20075255    28648823    25244985    28410430    23537049    24845553    20920257    23761445    20798317    21282665    21347285    24952583    21719571   

EMBL

BABH01020677    BABH01020678    BABH01020679    GQ426296    ADM32522.1    NWSH01000788    + More
PCG74196.1    KZ150018    PZC74936.1    PCG74197.1    KQ458761    KPJ05171.1    ODYU01010704    SOQ55983.1    KQ460820    KPJ12097.1    CH479179    EDW24211.1    CM000070    EAL28113.1    CP012526    ALC46598.1    KQ971321    EFA00185.1    AE014297    AGB95946.1    AB112473    AY075206    AAF55062.1    AAL68074.1    BAE72064.1    CH480815    EDW42185.1    CH933806    EDW15389.1    CM000364    EDX12944.1    CH940650    EDW67946.1    CH902617    EDV41495.1    CM000160    EDW97576.1    CH954181    EDV48887.1    CH477387    EAT42058.1    GAPW01002124    JAC11474.1    OUUW01000005    SPP80275.1    EAT42059.1    UFQS01000843    UFQT01000843    SSX07220.1    SSX27563.1    GEZM01075222    JAV64199.1    GDHF01016040    JAI36274.1    GAMC01003137    JAC03419.1    GBXI01016210    JAC98081.1    GFDL01010963    JAV24082.1    JXUM01105531    JXUM01105532    JXUM01105533    KQ564974    KXJ71478.1    CH964232    EDW81584.1    UFQT01000749    SSX26945.1    GAKP01008496    GAKP01008495    JAC50457.1    GDAI01002876    JAI14727.1    GDHF01016378    JAI35936.1    GFDL01010262    JAV24783.1    CH916374    EDV91516.1    JXJN01014221    CCAG010004854    ATLV01014525    KE524974    KFB38864.1    AAAB01008846    EAA06411.5    APCN01001235    JRES01000789    KNC28319.1    CVRI01000010    CRK88983.1    NEVH01004410    PNF39662.1    NNAY01001695    OXU23199.1    AXCM01000049    KQ434902    KZC11170.1    AJVK01023706    GFDF01004308    JAV09776.1    AJWK01026143    AXCN02001229    FX985289    BAX07302.1    APGK01041367    KB740993    ENN76049.1    KK852458    KDR23547.1    GGFJ01006413    MBW55554.1    GGFJ01006414    MBW55555.1    GGFJ01006415    MBW55556.1    GGFM01004966    MBW25717.1    ADMH02001819    ETN61021.1    GL442872    EFN62839.1    GGFK01008227    MBW41548.1    GGFK01009172    MBW42493.1    GL451499    EFN79184.1    KQ977963    KYM98420.1    KQ983238    KYQ46205.1    ACPB03009692    GFTR01005199    JAW11227.1    GL762022    EFZ22436.1    KQ979039    KYN23361.1    KQ981606    KYN39530.1    KQ976509    KYM82727.1    ADTU01000443    ADTU01000444    AFFK01018331    DS231903    EDS45136.1    KJ512094    AID60317.1    GL888633    EGI58691.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000008744    UP000001819    + More
UP000092553    UP000007266    UP000192221    UP000000803    UP000001292    UP000009192    UP000000304    UP000008792    UP000007801    UP000002282    UP000008711    UP000008820    UP000268350    UP000069940    UP000249989    UP000007798    UP000001070    UP000078200    UP000092443    UP000092460    UP000092444    UP000092445    UP000030765    UP000091820    UP000095300    UP000075882    UP000007062    UP000076407    UP000075903    UP000095301    UP000075902    UP000075840    UP000037069    UP000183832    UP000235965    UP000002358    UP000215335    UP000075883    UP000076502    UP000092462    UP000092461    UP000075880    UP000075884    UP000075886    UP000076408    UP000075900    UP000075901    UP000019118    UP000075920    UP000027135    UP000075881    UP000000673    UP000000311    UP000008237    UP000078542    UP000069272    UP000075809    UP000015103    UP000078492    UP000078541    UP000075885    UP000078540    UP000005205    UP000002320    UP000007755   

PRIDE

E9JEH9    Q9VFJ3    B4K835    B4PST0    B4N937    B4JTT7    + More

Pfam

PF01869   BcrAD_BadFG        + More
PF17820   PDZ_6
PF13180   PDZ_2

Interpro

IPR036034   PDZ_sf        + More
IPR002731   ATPase_BadF       
IPR001478   PDZ       
IPR041489   PDZ_6       
IPR009003   Peptidase_S1_PA       
IPR001940   Peptidase_S1C       

SUPFAM

SSF50494   SSF50494        + More
SSF50156   SSF50156       

ProteinModelPortal

E9JEH9   A0A2A4JQZ8   A0A2W1BPY2   A0A2A4JQ82   A0A194QNW3   A0A2H1WSF0   + More
A0A0N1IGF8   B4G316   Q297U2   A0A0M5J9A9   D6WGF2   A0A1W4USN6   A0A0B4KG52   Q9VFJ3   B4HEM8   B4K835   B4QZU6   B4LY58   B3LVG7   B4PST0   B3P3J9   Q176F8   A0A023ET05   A0A3B0JDW7   Q176F7   A0A1S4FDA2   A0A336L1Z8   A0A1Y1KS05   A0A0K8VBF4   W8C2G7   A0A0A1WGS0   A0A1Q3F965   A0A182H2K1   B4N937   A0A336M9M5   A0A034W811   A0A0K8TK95   A0A0K8VAR6   A0A1Q3FBA9   B4JTT7   A0A1A9UEQ4   A0A1A9X9Z1   A0A1B0BH89   A0A1B0G0K2   A0A1A9ZMD8   A0A084VLM0   A0A1A9WNT6   A0A1I8PU85   A0A182KS70   Q7QF03   A0A182XPG7   A0A182VJQ7   A0A1I8M2X9   A0A182UBC6   A0A182IAG4   A0A0L0CA47   A0A1J1HLP9   A0A2J7RFQ5   K7IR33   A0A232EY33   A0A182M124   A0A154PIY5   A0A1B0D3U5   A0A1L8DTK2   A0A1B0CSJ1   A0A182IKI5   A0A182N0B5   A0A182QEE8   A0A182XY18   A0A1V1FKJ0   A0A182RQ72   A0A182T6P8   N6T759   A0A182W597   A0A067RIC9   A0A182K9C3   A0A2M4BRC5   A0A2M4BR44   A0A2M4BR51   A0A2M3ZAZ8   W5JAK0   E2AUP0   A0A2M4ALR3   A0A2M4ANZ4   E2BYN0   A0A151IDG5   A0A182FJ30   A0A151WEF3   T1HZX5   A0A224XFH9   E9IAJ5   A0A195EE27   A0A195FFQ2   A0A182PR27   A0A195BEW1   A0A158NUD5   T1IQJ2   B0WE06   A0A068F678   F4X495  

PDB

5M3O     E-value=6.80177e-79,     Score=748

KEGG

100533207    K08669 HtrA serine peptidase 2 [EC:3.4.21.108] | (RefSeq) htra2; high temperature requirement protei

PATHWAY

04214    Apoptosis - fly - Bombyx mori (domestic silkworm)
04215    Apoptosis - multiple species - Bombyx mori (domestic silkworm)

GO

GO:0004252   GO:0016021   GO:0006915   GO:0031966   GO:0005758   GO:0016006   GO:0007283   GO:0035234   GO:0043065   GO:0005829   GO:2001056   GO:0007005   GO:0042981   GO:0008233   GO:0005739   GO:0005515   GO:0006508   GO:0004935   GO:0005215   GO:0006270   GO:0042555   GO:0003707   GO:0006281   GO:0006259  

Subcellular location

Mitochondrion intermembrane space   Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment. The extramitochondrial protein does not diffuse throughout the cytosol but stays near the mitochondria.   With evidence from 2 publications.
Mitochondrion membrane   Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment. The extramitochondrial protein does not diffuse throughout the cytosol but stays near the mitochondria.   With evidence from 2 publications.

Length:

391

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

24.77581

Exp number, first 60 AAs:

12.39054

Total prob of N-in:

0.15764

POSSIBLE N-term signal

sequence

outside

1  -  391

 

 

Pi

194.166632

Theta

158.875052

Tajima's D

1.213389

CLR

0.080148

CSRT

0.715714214289285

Interpretation

Uncertain