Silkworm Genome Informatics Database

Gene

KWMTBOMO15648

Pre Gene Modal

BGIBMGA010689

Annotation

PREDICTED:_E3_ubiquitin-protein_ligase_MARCH5_[Papilio_machaon]

Location

Bomo_Chr26(-):5205568-5231906 View in EpiBrowser

Full name

E3 ubiquitin-protein ligase MARCH5

Alternative Name

Membrane-associated RING finger protein 5
Membrane-associated RING-CH protein V
RING-type E3 ubiquitin transferase MARCH5
Mitochondrial ubiquitin ligase
RING finger protein 153

Location in the cell

PlasmaMembrane Reliability : 4.654

CDS

ATGTCGGGTGAATCTAATGAGTGTCATGGAACCAGCGTTAGTAGTTCACAAGCTTTGCAGCCAGCTGAAGATGAAAATGCCAAGTCTTGTTGGGTCTGCTTCGCTACAGAGGCAGACGATAAACTCGCGGCGTGGGTACAACCGTGTAAATGTATCGGAACAACAAAATGGGTTCATCAGTCATGTCTCCAAAGATGGGTCGATGAGAAGCAGCGGGGCAACATCACACGCAAGGTTCTCTGCCCTCAGTGTAAAGCGGAATACATTGTCGTGTTTCCGTCCATGGGTGCCTTCGTAGCCATGCTCGATGCTATGGAAGAGATCACCCACAAGATATGTCCATTTATTGCTGGTGGAGTGCTACTTGGCTCCATATATTGGATTGCCATTACATACGGAGCGGTCACTGTCATGCAGGTAGTAGGCCATCGCGAAGGTCTCGAGATGATGGAGTCGGCGGACCCCTTGGTTTTACTCGTGCTACTGCCCACGATCCCAGTGACCCTCATCAGTGCGAAGATGTATAACTGGGAGGATTCCGTTCTCCTGTTCCTCAGGAAGTACTGCGCTAAGATACCTGTCTTGGCTTATATTCTGCCTTTCGGAAACGTAGACAATGATCGCGCTATAGTGGCCGGTCAGACGACGCCACAGAACCCAAACGCGAGCCATCTATCCCCGACAAGGATATTCTGTTCGGCTCTGTTGCTACCAACGATATCGACGATCATAGGAAAGATATTCTTCCGTTCGATACAGAATAACCTCCACAGAACAATGCTCGGAGGCCTGACTTATATCACGTTGAAAGGGGCCTTGAAAATATATCACAAACAAATGCTGTACGTGAGGCATTCCAGTAGAAAGATATTGGATTACACGGAATCTAATTTGAAATTATACAGGAATTCGACAACCCTAGCGACCGATGCCGTTTCCAGTAGGGACGACAGCGAACAAGTCGTTTGA

Protein

MSGESNECHGTSVSSSQALQPAEDENAKSCWVCFATEADDKLAAWVQPCKCIGTTKWVHQSCLQRWVDEKQRGNITRKVLCPQCKAEYIVVFPSMGAFVAMLDAMEEITHKICPFIAGGVLLGSIYWIAITYGAVTVMQVVGHREGLEMMESADPLVLLVLLPTIPVTLISAKMYNWEDSVLLFLRKYCAKIPVLAYILPFGNVDNDRAIVAGQTTPQNPNASHLSPTRIFCSALLLPTISTIIGKIFFRSIQNNLHRTMLGGLTYITLKGALKIYHKQMLYVRHSSRKILDYTESNLKLYRNSTTLATDAVSSRDDSEQVV

Description

Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.

Catalytic Activity

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Subunit

Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.

Miscellaneous

By binding to and ubiquitinating two ALS1 variants of SOD1 (mSOD1 variants Arg-86 and Ala-94) it attenuates their cytotoxicity.

Keywords

Complete proteome Endoplasmic reticulum Membrane Metal-binding Mitochondrion Mitochondrion outer membrane Reference proteome Transferase Transmembrane Transmembrane helix Ubl conjugation Ubl conjugation pathway Zinc Zinc-finger

Feature

chain E3 ubiquitin-protein ligase MARCH5

Uniprot

A0A0N0PEH6 A0A194QCM8 A0A2A4K3M3 S4PB30 A0A0L7LHP4 A0A2H1VT43 + More
A0A212FDD8 A0A2P8YLE4 E2ACF1 A0A195CSM3 A0A195E2Y8 A0A151JYB4 A0A151WKE6 A0A2J7RFK2 F4X2E0 A0A026W793 A0A0T6AYV8 E2C3C6 A0A1W4WKJ4 A0A0J7N3M3 A0A1L8DSM4 A0A0C9S2M3 A0A067QL61 N6TWA5 A0A1I8M762 A0A151I0R4 A0A0L7QUW5 A0A1Y1JUC2 A0A088A2T3 A0A1I8Q051 A0A0N0BHJ0 A0A1A9WZ19 A0A2A3ECH4 A0A1B0AJX1 A0A240SWY3 A0A1A9UWY2 A0A154PU19 A0A1W4WKH2 E0V9S6 G3URQ1 F7EEW5 A0A1B0AL61 D6WZW6 A0A3Q7PSZ0 A0A2R9CEI2 V4AT16 A0A1S3GPB8 A0A0N8EUL7 A0A287DE71 A0A1U7QD46 A0A250YEJ3 A0A286XMX9 B0BNF6 A2RTC8 A0A2K6CYS2 A0A384CHZ6 G1SPH8 K9IX32 G3RZ06 G1RQ17 A0A341B0F7 A0A287A6L2 A0A2K5XDY0 A0A0D9R2M2 U6CTI2 F7DKE7 A0A2Y9D8J6 A0A2U4C408 A0A096P5I9 A0A2K5DUU8 A0A2Y9EU78 F7G4B1 A0A2K6RMD1 A0A2Y9HTT5 A0A2U3XR95 A0A2I2U6T9 A0A2K5KSL1 A0A3Q7RWE9 A0A1S2ZBL8 A0A2Y9MSV7 A0A2U3WPN3 A0A2K5H8Y5 A0A2K5SID1 A0A2K6USI4 A0A2K6G2K9 A0A3Q7V3L5 H2NB06 G7PDK0 H2R4C6 F6QQS0 U3DPN3 Q3KNM2 Q9NX47 Q0X0A5 Q3ZC24 A0A0B6ZBJ0 A0A1L8FJ74 M3Z0K6 A0A151NZV0

EC Number

2.3.2.27

Pubmed

26354079 23622113 26227816 22118469 29403074 20798317 + More
21719571 24508170 24845553 23537049 25315136 28004739 20566863 20838655 18464734 18362917 19820115 22722832 23254933 28087693 21993624 15489334 15057822 15632090 12040188 21183079 24813606 22398555 19892987 17495919 25362486 17975172 22002653 16136131 17431167 25319552 25243066 16141072 16936636 14702039 15164054 14722266 16874301 17606867 19741096 20103533 21269460 22814378 24275569 25944712 27762356 22293439

EMBL

KQ459941 KPJ19163.1 KQ459193 KPJ03174.1 NWSH01000220 PCG78270.1 + More
GAIX01004771 JAA87789.1 JTDY01001030 KOB75083.1 ODYU01004268 SOQ43958.1 AGBW02009060 OWR51769.1 PYGN01000512 PSN45061.1 GL438494 EFN68890.1 KQ977329 KYN03527.1 KQ979701 KYN19513.1 KQ981477 KYN41443.1 KQ983028 KYQ48155.1 NEVH01004410 PNF39614.1 GL888578 EGI59365.1 KK107364 EZA51925.1 LJIG01022500 KRT80242.1 GL452318 EFN77572.1 LBMM01010715 KMQ87300.1 GFDF01004710 JAV09374.1 GBYB01015156 JAG84923.1 KK853305 KDR08720.1 APGK01052696 KB741213 KB631874 ENN72671.1 ERL86868.1 KQ976606 KYM79326.1 KQ414731 KOC62428.1 GEZM01102898 JAV51741.1 KQ435750 KOX76292.1 KZ288293 PBC28992.1 CCAG010007645 KQ435130 KZC14858.1 DS234996 EEB10132.1 JXJN01030051 KQ971372 EFA10490.1 AJFE02089241 AJFE02089242 AJFE02089243 AJFE02089244 AJFE02089245 AJFE02089246 AJFE02089247 AJFE02089248 AJFE02089249 AJFE02089250 KB201362 ESO96851.1 GEBF01001227 JAO02406.1 AGTP01072989 GFFW01002814 JAV41974.1 AAKN02053761 AAKN02053762 AC103056 BC158801 CH473953 AAI58802.1 EDM13194.1 BC132455 BC132457 CH466534 AAI32456.1 EDL41781.1 AAGW02036317 AAGW02036318 AAGW02036319 GABZ01007359 JAA46166.1 CABD030075335 CABD030075336 CABD030075337 CABD030075338 CABD030075339 CABD030075340 ADFV01114829 ADFV01114830 ADFV01114831 ADFV01114832 ADFV01114833 ADFV01114834 ADFV01114835 ADFV01114836 AEMK02000096 AQIB01042863 AQIB01042864 AQIB01042865 HAAF01001798 CCP73624.1 AHZZ02035779 AHZZ02035780 AANG04000273 ABGA01344827 ABGA01344828 ABGA01344829 ABGA01344830 ABGA01344831 ABGA01344832 ABGA01344833 ABGA01344834 ABGA01344835 ABGA01344836 NDHI03003280 PNJ89437.1 AQIA01071989 AQIA01071990 AQIA01071991 AQIA01071992 CM001284 EHH64882.1 AACZ04051057 GABC01009800 GABC01009799 GABF01001453 GABD01007721 GABE01001630 NBAG03000216 JAA01538.1 JAA20692.1 JAA25379.1 JAA43109.1 PNI82326.1 JSUE03043984 JSUE03043985 JSUE03043986 JU320794 JU471454 JU471455 JV047445 CM001261 AFE64550.1 AFH28258.1 AFI37516.1 EHH19228.1 GAMT01000136 GAMS01007764 GAMR01007664 GAMQ01003536 GAMP01006224 JAB11725.1 JAB15372.1 JAB26268.1 JAB38315.1 JAB46531.1 AK003478 AK009232 BC064752 BC107215 AB191202 AK000452 AL158040 AL161652 BC015480 AB212856 BC102966 HACG01018421 CEK65286.1 CM004478 OCT71622.1 AEYP01026409 AEYP01026410 AEYP01026411 AEYP01026412 AEYP01026413 AEYP01026414 AEYP01026415 AEYP01026416 AEYP01026417 AEYP01026418 AKHW03001467 KYO42294.1

Proteomes

UP000053240 UP000053268 UP000218220 UP000037510 UP000007151 UP000245037 + More
UP000000311 UP000078542 UP000078492 UP000078541 UP000075809 UP000235965 UP000007755 UP000053097 UP000008237 UP000192223 UP000036403 UP000027135 UP000019118 UP000030742 UP000095301 UP000078540 UP000053825 UP000005203 UP000095300 UP000053105 UP000091820 UP000242457 UP000092445 UP000092444 UP000078200 UP000076502 UP000009046 UP000001645 UP000002279 UP000092460 UP000007266 UP000286641 UP000240080 UP000030746 UP000081671 UP000005215 UP000189706 UP000005447 UP000002494 UP000233120 UP000261680 UP000291021 UP000001811 UP000001519 UP000001073 UP000252040 UP000008227 UP000233140 UP000029965 UP000002281 UP000248480 UP000245320 UP000028761 UP000233020 UP000248484 UP000002280 UP000233200 UP000248481 UP000245341 UP000011712 UP000233060 UP000286640 UP000079721 UP000248483 UP000245340 UP000233080 UP000233040 UP000233220 UP000233160 UP000286642 UP000001595 UP000009130 UP000233100 UP000002277 UP000006718 UP000008225 UP000000589 UP000005640 UP000009136 UP000186698 UP000000715 UP000050525

PRIDE

Q3KNM2 Q9NX47

Pfam

PF12906 RINGv + More
PF03345 DDOST_48kD

Interpro

IPR013083   Znf_RING/FYVE/PHD        + More
IPR011016   Znf_RING-CH
IPR005013   DDOST_48_kDa_subunit

Gene 3D

3.30.40.10

ProteinModelPortal

A0A0N0PEH6 A0A194QCM8 A0A2A4K3M3 S4PB30 A0A0L7LHP4 A0A2H1VT43 + More
A0A212FDD8 A0A2P8YLE4 E2ACF1 A0A195CSM3 A0A195E2Y8 A0A151JYB4 A0A151WKE6 A0A2J7RFK2 F4X2E0 A0A026W793 A0A0T6AYV8 E2C3C6 A0A1W4WKJ4 A0A0J7N3M3 A0A1L8DSM4 A0A0C9S2M3 A0A067QL61 N6TWA5 A0A1I8M762 A0A151I0R4 A0A0L7QUW5 A0A1Y1JUC2 A0A088A2T3 A0A1I8Q051 A0A0N0BHJ0 A0A1A9WZ19 A0A2A3ECH4 A0A1B0AJX1 A0A240SWY3 A0A1A9UWY2 A0A154PU19 A0A1W4WKH2 E0V9S6 G3URQ1 F7EEW5 A0A1B0AL61 D6WZW6 A0A3Q7PSZ0 A0A2R9CEI2 V4AT16 A0A1S3GPB8 A0A0N8EUL7 A0A287DE71 A0A1U7QD46 A0A250YEJ3 A0A286XMX9 B0BNF6 A2RTC8 A0A2K6CYS2 A0A384CHZ6 G1SPH8 K9IX32 G3RZ06 G1RQ17 A0A341B0F7 A0A287A6L2 A0A2K5XDY0 A0A0D9R2M2 U6CTI2 F7DKE7 A0A2Y9D8J6 A0A2U4C408 A0A096P5I9 A0A2K5DUU8 A0A2Y9EU78 F7G4B1 A0A2K6RMD1 A0A2Y9HTT5 A0A2U3XR95 A0A2I2U6T9 A0A2K5KSL1 A0A3Q7RWE9 A0A1S2ZBL8 A0A2Y9MSV7 A0A2U3WPN3 A0A2K5H8Y5 A0A2K5SID1 A0A2K6USI4 A0A2K6G2K9 A0A3Q7V3L5 H2NB06 G7PDK0 H2R4C6 F6QQS0 U3DPN3 Q3KNM2 Q9NX47 Q0X0A5 Q3ZC24 A0A0B6ZBJ0 A0A1L8FJ74 M3Z0K6 A0A151NZV0

PDB

2D8S E-value=0.000666952, Score=101

KEGG

101736006 K10660 E3 ubiquitin-protein ligase MARCH5 [EC:2.3.2.27] | (RefSeq) E3 ubiquitin-protein ligase MARCH

GO

GO:0016874 GO:0008270 GO:0016021 GO:0018279 GO:0005789 GO:0090141 GO:0051865 GO:0051020 GO:0004842 GO:0000209 GO:0090344 GO:0005741 GO:0070585 GO:0046872 GO:0005739 GO:0090140 GO:0005783 GO:0016020 GO:0006289 GO:0006355 GO:0020037 GO:0030117 GO:0006418 GO:0003707 GO:0006281 GO:0006259

Subcellular location

Mitochondrion outer membrane
Endoplasmic reticulum membrane

Length:

322

Number of predicted TMHs:

4

Exp number of AAs in TMHs:

80.4385599999999

Exp number, first 60 AAs:

0.0007

Total prob of N-in:

0.85223

inside

1 - 87

TMhelix

88 - 105

outside

106 - 119

TMhelix

120 - 142

inside

143 - 154

TMhelix

155 - 177

outside

178 - 226

TMhelix

227 - 249

inside

250 - 322

Pi

224.367619

Theta

159.934281

Tajima's D

1.217099

CLR

0.447024

CSRT

0.71596420178991

Interpretation

Uncertain

Sequence

Summary

Ontologies

Topology

Population Genetic Test Statistics