Pre Gene Modal
BGIBMGA010689
Annotation
PREDICTED:_E3_ubiquitin-protein_ligase_MARCH5_[Papilio_machaon]
Full name
E3 ubiquitin-protein ligase MARCH5
Alternative Name
Membrane-associated RING finger protein 5
Membrane-associated RING-CH protein V
RING-type E3 ubiquitin transferase MARCH5
Mitochondrial ubiquitin ligase
RING finger protein 153
Location in the cell
PlasmaMembrane   Reliability : 4.654
Sequence
CDS
ATGTCGGGTGAATCTAATGAGTGTCATGGAACCAGCGTTAGTAGTTCACAAGCTTTGCAGCCAGCTGAAGATGAAAATGCCAAGTCTTGTTGGGTCTGCTTCGCTACAGAGGCAGACGATAAACTCGCGGCGTGGGTACAACCGTGTAAATGTATCGGAACAACAAAATGGGTTCATCAGTCATGTCTCCAAAGATGGGTCGATGAGAAGCAGCGGGGCAACATCACACGCAAGGTTCTCTGCCCTCAGTGTAAAGCGGAATACATTGTCGTGTTTCCGTCCATGGGTGCCTTCGTAGCCATGCTCGATGCTATGGAAGAGATCACCCACAAGATATGTCCATTTATTGCTGGTGGAGTGCTACTTGGCTCCATATATTGGATTGCCATTACATACGGAGCGGTCACTGTCATGCAGGTAGTAGGCCATCGCGAAGGTCTCGAGATGATGGAGTCGGCGGACCCCTTGGTTTTACTCGTGCTACTGCCCACGATCCCAGTGACCCTCATCAGTGCGAAGATGTATAACTGGGAGGATTCCGTTCTCCTGTTCCTCAGGAAGTACTGCGCTAAGATACCTGTCTTGGCTTATATTCTGCCTTTCGGAAACGTAGACAATGATCGCGCTATAGTGGCCGGTCAGACGACGCCACAGAACCCAAACGCGAGCCATCTATCCCCGACAAGGATATTCTGTTCGGCTCTGTTGCTACCAACGATATCGACGATCATAGGAAAGATATTCTTCCGTTCGATACAGAATAACCTCCACAGAACAATGCTCGGAGGCCTGACTTATATCACGTTGAAAGGGGCCTTGAAAATATATCACAAACAAATGCTGTACGTGAGGCATTCCAGTAGAAAGATATTGGATTACACGGAATCTAATTTGAAATTATACAGGAATTCGACAACCCTAGCGACCGATGCCGTTTCCAGTAGGGACGACAGCGAACAAGTCGTTTGA
Protein
MSGESNECHGTSVSSSQALQPAEDENAKSCWVCFATEADDKLAAWVQPCKCIGTTKWVHQSCLQRWVDEKQRGNITRKVLCPQCKAEYIVVFPSMGAFVAMLDAMEEITHKICPFIAGGVLLGSIYWIAITYGAVTVMQVVGHREGLEMMESADPLVLLVLLPTIPVTLISAKMYNWEDSVLLFLRKYCAKIPVLAYILPFGNVDNDRAIVAGQTTPQNPNASHLSPTRIFCSALLLPTISTIIGKIFFRSIQNNLHRTMLGGLTYITLKGALKIYHKQMLYVRHSSRKILDYTESNLKLYRNSTTLATDAVSSRDDSEQVV
Summary
Description
Mitochondrial E3 ubiquitin-protein ligase that plays a crucial role in the control of mitochondrial morphology by acting as a positive regulator of mitochondrial fission. May play a role in the prevention of cell senescence acting as a regulator of mitochondrial quality control. Promotes ubiquitination of FIS1, DNM1L and MFN1.
Catalytic Activity
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
Subunit
Monomer and homodimer. Interacts with MFN1, MFN2, DNM1L and FIS1.
Miscellaneous
By binding to and ubiquitinating two ALS1 variants of SOD1 (mSOD1 variants Arg-86 and Ala-94) it attenuates their cytotoxicity.
Keywords
Complete proteome
Endoplasmic reticulum
Membrane
Metal-binding
Mitochondrion
Mitochondrion outer membrane
Reference proteome
Transferase
Transmembrane
Transmembrane helix
Ubl conjugation
Ubl conjugation pathway
Zinc
Zinc-finger
Feature
chain E3 ubiquitin-protein ligase MARCH5
PDB
2D8S
E-value=0.000666952,
Score=101
Ontologies
Topology
Subcellular location
Mitochondrion outer membrane
Endoplasmic reticulum membrane
Number of predicted TMHs:
4
Exp number of AAs in TMHs:
80.4385599999999
Exp number, first 60 AAs:
0.0007
Total prob of N-in:
0.85223
Population Genetic Test Statistics