SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO15583  Validated by peptides from experiments
Pre Gene Modal
BGIBMGA010725
Annotation
coatomer_protein_complex_subunit_alpha_[Bombyx_mori]
Full name
Coatomer subunit alpha      
Alternative Name
Alpha-coat protein
HEP-COP
Location in the cell
Cytoplasmic   Reliability : 2.249
 

Sequence

CDS
ATGTCGTTGAACCACGCCGAGTGGTGCGTGCTGGTGACGTGGCGCAACGGTGAGAACTGCTCTTACGAGCTGTACGCCGCGCCCCGGGACCACAGCGGCGCCGTGCCCGAGGGTACGGAGCCGGCCAGGGGGCAGGCCACCACCGCCATCTGGGTCGCCAGGAATCGATTCGCCGCACTAGAGAAGAACAACCAGCTTGTGATCAGGAATCTGAAGAACGAAGTGTCGAAGAAGATCTCGACTCCGACTTGCGAGGAGATCATGTACGCCGGCACGGGGATGCTGCTGCTACGCGAAGCCGACTCCGTGCAGCTGCTCGACGTACAACAGAAGAGGACCATTGCTTCCGTGAAAGTATCGAAATGTCGTTACGCCATTTGGAATACCGATATGTCAATAGTGGCTCTGTTGGGCAAGCACACGGTCACACTATGCACCAAGAAGCTGGAGCAGCTGTGCTGCATCACCGAAGGGGCCAGAGTCAAGTCCGGCGCCTTCGACGATTCCACACCGCAGCCCGTGTTCATATACACCACGGCAAATCACATCAAGTACTGCTGTAAGGACGGCGATTACGGCATCATCCGCACTCTGGACGTGCCCGTGTACGCGGTGCGCGTGCTGTCGACGGAGACGGGCGCGCGCGTGGTGTGCTTGGACCGAGAATGCAGGCCCAAAGTACTCAACATCGACCCCACGGAATACAGATTCAAATTAGCTCTAGTGACGCGCCAATACGATCAGGTGTTGCACATGGTGCGGACGGCTAAACTTGTCGGGCAGAGCATCATCGCCTACTTGCAGGAGAAGGGCTATCCCGAAGTGGCGCTTCATTTCGTGAAGGACTCCCGCACGCGTCTCTCGTTGGCCCTGCAGTGCGGGAACATCGAGGTGGCTCTGGAGGCGGCCAAGAGTTTGGACGAGCCAGCCGCCTGGGACCAGCTGGCCAAAGCCGCCCTCACGACTGGGAACCACCAGATCGTCGAGATGTGCTACCAGCGCACGAAGAACTTCGACAAGCTCTCGTTCCTGTACTTGGTGACCGGCAATCTGGAGAAGCTGCGCAAGATGATGAAGATAGCCGAGATAAGGAAGGACACCTCGGCCCAGTTCCAAGGGGCGCTGCTCCTCGGCGACGTCGGCGAACGGATAAGGCTGCTGAGGAACTCGGGACAGCTGTCCCTGGCGTATCTGACGGCGATCAACCACAAGCAAGTGGAGGAGGCGGAGCAGCTGAAGGCAGCCTTGGAGGCAGCCGGCATGCCGGTCCCGGAGGCGAACCCCGACGCGGTGTTCCTCCGCCCGCCGCTGCCCATACAGAGGAACCAGCCTAACTGGCCCCTGCTCGCCGTCTCCAAGAGTTTCTTCGAAGTGGCCGGACAAGCGCGGGCGGCGGCCGAGGGCTCCGGCAGCGCGGTGGCGGCCGCCCTCGACGAGCCCTTGGAGGCCGCGGGCGCCTGGGGAGACGACGACGTGCTGCCCGACCATAAGGAAGAAGGCGAAGAGGAGATCATGGAGGACGCCTGCGAAGATGGCGGCTGGGACGTCGGTGATGAAGACCTGGAGCTTCCCGAGGAGCTGGCGCCCGTGTCGGCGGACATGGGCGCCGCGGAGGACTCGGAGCAGTACTTCGTGGCCCCCACGCGCGGAGCCTCCGCCCCCCTCGCCGCGCGCCTGCGGACGGCGCACGACCACGTGGCCACCGGACAGTTTGAGGCCGCCATGAGATTGTTGAACGAGCAAGTCGGAATCGTGAACTTTGCCCCTTACGAGTCTGTGTTCGCGGAGATGTTCGCGCACGCTCGCGTTACGTTCGGCGCGCTGCCCTCGCTGCCCGCGCTCACTGCGTACCTGCACAGGAATTGGAAGGAAGCCACCGGGAAGGATCTCCTGCCTGTCATAACGTTGAAGCTAAGCGACTTGGTGAGTCAGCTGCAGCAGAGCTACCAGTTGACCACGGCGGGCCGGTTCCCCGAGGCCATAGAGCGGCTGCAGGGCGTGGCGCAGCGCGTGCCGCTGCTGCTGGTGGACTCCAAGGCCGAGCTGTCCGAGGCCCAACAGTTATTGGCCGTCTGCAGGGACTACCTCGTGGGACTCGCCATGGAGACCGCCAGGAAAGCGATGCCGAAGAACACCGTCGACGAACAGAAACGTACCTGCGAGATGGCCGCCTACTTCACGCACTGCAAGCTCCAGCCCGTGCACCAGATCCTCACGCTGCGGACGGCCCTCAACATGTTCTTCAAGCTGAAGAACTACCGCACGGCCGCTTCGTTCGCGAGGCGCCTCCTAGAGCTCGGACCCCGGCCTGAGGTCGCCCAGCAAGCGCGGAAGATCCTCCAGGCCTGCGAGAAGACCCCGACTGATGAGCACCAGCTGCTCTACGACGAGCATAATCCATTCAGCGTCTGCGGCATCAGCTACAAGCCCATCTACCGCGGCAAGCCGGAGGAAAAGTGCTCGCTGTGCGCGGCTAGCTTCATGCCTGAACACAAGGGGAAGCTGTGCCCGGTGTGCGGGGTCGCCGAGATCGGCAAGGACGCGCTCGGCCTCCGTATCTGTCCCTTACAGTTCAACAGATAG
Protein
MSLNHAEWCVLVTWRNGENCSYELYAAPRDHSGAVPEGTEPARGQATTAIWVARNRFAALEKNNQLVIRNLKNEVSKKISTPTCEEIMYAGTGMLLLREADSVQLLDVQQKRTIASVKVSKCRYAIWNTDMSIVALLGKHTVTLCTKKLEQLCCITEGARVKSGAFDDSTPQPVFIYTTANHIKYCCKDGDYGIIRTLDVPVYAVRVLSTETGARVVCLDRECRPKVLNIDPTEYRFKLALVTRQYDQVLHMVRTAKLVGQSIIAYLQEKGYPEVALHFVKDSRTRLSLALQCGNIEVALEAAKSLDEPAAWDQLAKAALTTGNHQIVEMCYQRTKNFDKLSFLYLVTGNLEKLRKMMKIAEIRKDTSAQFQGALLLGDVGERIRLLRNSGQLSLAYLTAINHKQVEEAEQLKAALEAAGMPVPEANPDAVFLRPPLPIQRNQPNWPLLAVSKSFFEVAGQARAAAEGSGSAVAAALDEPLEAAGAWGDDDVLPDHKEEGEEEIMEDACEDGGWDVGDEDLELPEELAPVSADMGAAEDSEQYFVAPTRGASAPLAARLRTAHDHVATGQFEAAMRLLNEQVGIVNFAPYESVFAEMFAHARVTFGALPSLPALTAYLHRNWKEATGKDLLPVITLKLSDLVSQLQQSYQLTTAGRFPEAIERLQGVAQRVPLLLVDSKAELSEAQQLLAVCRDYLVGLAMETARKAMPKNTVDEQKRTCEMAAYFTHCKLQPVHQILTLRTALNMFFKLKNYRTAASFARRLLELGPRPEVAQQARKILQACEKTPTDEHQLLYDEHNPFSVCGISYKPIYRGKPEEKCSLCAASFMPEHKGKLCPVCGVAEIGKDALGLRICPLQFNR

Summary

Description
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network.
The coatomer is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles, which further mediate biosynthetic protein transport from the ER, via the Golgi up to the trans Golgi network. Coatomer complex is required for budding from Golgi membranes, and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, the coatomer can only be recruited by membranes associated to ADP-ribosylation factors (ARFs), which are small GTP-binding proteins; the complex also influences the Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).
Xenin stimulates exocrine pancreatic secretion. It inhibits pentagastrin-stimulated secretion of acid, to induce exocrine pancreatic secretion and to affect small and large intestinal motility. In the gut, xenin interacts with the neurotensin receptor (By similarity).
Subunit
Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits.
Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1. Interacts with JAGN1 (By similarity).
Oligomeric complex that consists of at least the alpha, beta, beta', gamma, delta, epsilon and zeta subunits. Probably interacts with PEX11A. Interacts with SCYL1 (By similarity). Interacts with JAGN1.
Keywords
3D-structure   Complete proteome   Cytoplasm   Cytoplasmic vesicle   Direct protein sequencing   ER-Golgi transport   Golgi apparatus   Hormone   Membrane   Methylation   Phosphoprotein   Protein transport   Reference proteome   Repeat   Secreted   Transport   WD repeat   Alternative splicing   Disease mutation   Polymorphism   RNA editing  
Feature
chain  Coatomer subunit alpha
peptide  Proxenin
splice variant  In isoform 2.
sequence variant  In RNA edited version.
EMBL
GU322815    ADB66734.1    BABH01016793    BABH01016794    ODYU01003431    SOQ42188.1    + More
NWSH01000595    PCG75453.1    PCG75452.1    RSAL01000221    RVE44062.1    GANO01000743    JAB59128.1    ATLV01020668    KE525304    KFB45492.1    GFDF01003493    JAV10591.1    AAAB01008980    EAA14358.1    APCN01005265    CH477974    EAT34698.1    JXUM01056641    JXUM01056642    KQ561919    KXJ77156.1    GBHO01004829    GDHC01020868    JAG38775.1    JAP97760.1    NEVH01003779    PNF39949.1    GFDL01011243    JAV23802.1    PNF39948.1    GDAI01000389    JAI17214.1    GDAI01001856    JAI15747.1    AXCM01000224    KU932326    APA33962.1    DS232122    EDS35527.1    KK852597    KDR20569.1    DS235408    EEB15564.1    GFTR01008671    JAW07755.1    AXCN02001866    RCHS01001746    RMX51607.1    LSMT01000034    PFX31523.1    GBGD01000164    JAC88725.1    KZ288324    PBC28090.1    KQ414775    KOC61310.1    UFQS01000912    UFQT01000912    SSX07627.1    SSX27965.1    KQ434807    KZC06156.1    UFQS01000047    UFQT01000047    SSW98541.1    SSX18927.1    GALX01003057    JAB65409.1    KQ435794    KOX73744.1    AKCR02000219    PKK18229.1    GDKW01000789    JAI55806.1    ACPB03025161    X96768    BC153251    CH478573    EAT32766.1    AJFE02037595    AJFE02037596    AACZ04025375    KA647729    AFP62358.1    AAEX03018465    AAEX03018466    GGFK01001252    MBW34573.1    KB016438    EPY89012.1    AGTP01101573    AGTP01101574    AGTP01101575    AGTP01101576    AGTP01101577    AGTP01101578    AAKN02043110    AAGW02000288    AAGW02000289    GABF01000900    GABF01000899    GABF01000897    JAA21246.1    KB031109    ELK03355.1    GABE01001289    NBAG03000493    JAA43450.1    PNI19746.1    BC082785    AAH82785.1    JH172217    EHB13664.1    JH000204    EGV93396.1    AEMK02000022    AMGL01005150    AMGL01005151    AMGL01005152    AK043479    BAC31555.1    GEBF01001956    JAO01677.1    AEYP01087984    AEYP01087985    AEYP01087986    AEYP01087987    GBYB01004627    GBYB01004628    GBYB01004629    JAG74394.1    JAG74395.1    JAG74396.1    AQIA01002081    AC158930    BC024070    BC047429    U24105    AL513282    AL445230    CH471121    BC038447    AABR07021770    AABR07021771    AABR07021772    AABR07072344    AC095300    CH473985    EDL94678.1   
Pfam
PF12894   ANAPC4_WD40        + More
PF04053   Coatomer_WDAD
PF00400   WD40
PF06957   COPI_C
PF00324   AA_permease
PF00248   Aldo_ket_red
PF03522   SLC12
Interpro
IPR015943   WD40/YVTN_repeat-like_dom_sf        + More
IPR001680   WD40_repeat       
IPR011048   Haem_d1_sf       
IPR024977   Apc4_WD40_dom       
IPR017986   WD40_repeat_dom       
IPR006692   Coatomer_WD-assoc_reg       
IPR020472   G-protein_beta_WD-40_rep       
IPR010714   Coatomer_asu_C       
IPR036322   WD40_repeat_dom_sf       
IPR016391   Coatomer_asu       
IPR019775   WD40_repeat_CS       
IPR004841   AA-permease/SLC12A_dom       
IPR018170   Aldo/ket_reductase_CS       
IPR023210   NADP_OxRdtase_dom       
IPR018491   SLC12_C       
IPR036812   NADP_OxRdtase_dom_sf       
IPR020471   Aldo/keto_reductase       
IPR011044   Quino_amine_DH_bsu       
SUPFAM
SSF50978   SSF50978        + More
SSF51004   SSF51004       
SSF51430   SSF51430       
SSF50969   SSF50969       
Gene 3D
PDB
5NZV     E-value=0,     Score=2581

Ontologies

Topology

Subcellular location
Cytoplasm  
Golgi apparatus membrane  
Cytoplasmic vesicle   The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.   With evidence from 2 publications.
COPI-coated vesicle membrane   The coatomer is cytoplasmic or polymerized on the cytoplasmic side of the Golgi, as well as on the vesicles/buds originating from it.   With evidence from 2 publications.
Secreted  
Length:
860
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.02886
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00036
outside
1  -  860
 
 

Population Genetic Test Statistics

Pi
292.742926
Theta
159.899594
Tajima's D
2.772541
CLR
0.362671
CSRT
0.968801559922004
Interpretation
Uncertain
Peptides ×
Source Sequence Identity Evalue
31250652 LLLLRHMVHVHAPAR 100.00 5e-25
27102218 TIDVPVYAVR 100.00 4e-10
28467696 SGADTIVACEAFQPMAECCIR 100.00 4e-10
28556443 LVGQSIIAYLQEK 100.00 4e-10
28556443 TLDVPVYAVR 100.00 4e-09
31250652 SVAPGPAGLTEHLRNPQATDLFGQLVIR 100.00 5e-06
28556443 DHSGAVPEGTEPAR 100.00 2e-05
28556443 GVNWACFHPTLPLIASAADDR 100.00 2e-05
26822097 TCEMAAYFTHCK 100.00 1e-04
26280517 TAGYNNVNVR 100.00 1e-04
26822097 AAIEAAGMPVPEANPDAVFIRPPIPIQR 100.00 0.002
26280517 DFYFGK 100.00 0.002
25044914 DHSEVIQSTSWK 100.00 0.002
27102218 QYDQVIHMVR 100.00 0.002
28467696 DHSDVSNQIYACYAIGK 100.00 0.002
28556443 LVTGNLEK 100.00 0.002
28556443 VLEGILNDYMDLQYAK 100.00 0.002
26822097 SIDEPAAWDQIAK 100.00 0.009
25044914 TCDKPWSYANMTAEAPICCENSQVK 100.00 0.009
27102218 TTFFHHEYPWIISASDDQTIR 100.00 0.009
28556443 LVGQSIIAYLQEK 100.00 0.024
28556443 SVAPGPAGLTEHLR 100.00 0.024
28556443 SFFEVAGQAR 100.00 0.041
28556443 SGAFDDSTPQPVFIYTTANHIK 100.00 0.041
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