Gene

KWMTBOMO15359  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA005116

Annotation

PREDICTED:_bifunctional_glutamate/proline--tRNA_ligase_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr25(-):10059238-10070096      View in EpiBrowser

Full name

Bifunctional glutamate/proline--tRNA ligase      

Alternative Name

Bifunctional aminoacyl-tRNA synthetase

Location in the cell

Cytoplasmic   Reliability : 2.196

CDS

ATGATGCGGGTATCTTGCAATAGATCTAACCCGCCTTTAGGTGGTTTGATGGCTGTAGAATTTTACAGATCGTCGGCAAAAGAAATAGACATCGTTTGGGAGAATGACTCAGTTATTATTCTTCCAAATTCCACCAAACCAGTGCCATTTGGGACTAGCAATGATTTGATTAGAATTCTTGAAAATACCTTTAACAAGTCTGTGAGTCCACTTGAGAAAGTCACAATGAATCATTGGTTGTCTTTCAGTCTTATATTGGAAGATGAAATACCCAAGTCTGTTGAGTATTTAGATAAAACACTAGGGCCACTAACCTATTTGGTTGGTGAAACTTTATCTGTATCAGACTTTGCGGTCTTCAGTGTTCTTTACGTATCAAGTAAATTCAAAGAAATTTCAAAAGTGATTCCTTCTCAAAACATAAGACGCTGGATGAAATTGGTAGAAGCTCAACCGCAAGTCCAAACAGCTATAAAGGCTCTGCCGTCTGATGCCCTTGACAAACTTACCAAAGTTTCCTCCAGGAAATCACCACCAACAAATGCTGAATCAGGAGGTAGACAACAGGAAGGAAAGTTCATTGAACTCCCTCATGCTGAAATGGGAAAAGTTGTTGTAAGATTTCCACCTGAAGCTTCAGGATATCTTCATATTGGTCATGCAAAAGCGGCTCTTCTTAATCAATATTACCAGCAAGCCTTTCATGGCAAATTGGTCATGAGATTTGATGACACAAATCCAGCTAAAGAAAATGCAGATTTTGAAAAGGTTATTTTAGAGGATGTAGCAATGCTCGAAATAAAACCAGATATGTTCACACACACGTCCCAATACTTTGATTTAATGATGGAGTATTGTGAGAAACTCATCCGTGAAAACAAAGCTTTTGTGGATGATACTCCTGCTGAACAAATGAAAACTGAGAGAGAACAGAAAATTGAAAGCAAAAATAGAAACAATTCCGTAGAAAGGAACCTGCAACTGTGGGAAGAGATGAAGAAAGGCAGTAATATTGGTGTCCAATGTTGTGTACGAGCTAAAATAGACATGCAGTCAGCAAATGGTTGCCTGCGTGATCCCACCATTTACAGGTGCAAGCCTGAAGAACACCCAAGAACCGGCACACAATATAAAGTTTATCCGACCTACGACTTCGCGTGTCCAATCGTGGATTCCATAGAAGGGGTAACTCACGTCCTGCGAACGATGGAGTATCATGATCGAGACCCGCAGTTCTACTGGTTCATAGAGGCACTTGGTATTAGAAAACCTTATATTTGGGAGTATAGTAGATTGAGCATGACAAACACTGTACTGTCGAAAAGGAAACTAACTTGGTTTGTGGAACAAGGACTTGTAGATGGATGGGATGACCCTCGTATGCCAACAGTACGTGGAGTGCTGCGACGCGGTATGACCGTGGAAGCGCTACGTCAGTTTATTCACGCTCAAGGCTCCAGCAGGTCCGTGGTGTTCATGGAATGGGATAAAATTTGGGCAATCAATAAGAAGGTCATAGATCCGGTGGCTCCTCGCTACACAGCATTAGAAACAAACCCTGTTCCTGTGAATCTTAAGGGTGTCACAAGCGACAGCAGCTTAACTGTACCCCTGCATCCTAAAAATCCAGATGTTGGTACCAAAACTATATGGGTGGCTCCCAGGTTACTAATAGACCAGGCTGATGCGAAGACCTTGAAAGAAGGAGAAAACGCCACGTTTATTAACTATGGCAATGTTACCATCGATAAGATTCACAGATCAAAGGATGGTACAGTAACAAGTATAGATGGAACACCGAATCTCGATAATAAAGACTACAAAAAGACCGTCAAGTTGACTTGGCTCGCTGAGAGCAAACAATCACCAATGGTAGAGACCTATTGTGTTTACTTCGATCATATCATATCTAAAGCAGTGCTGGGCAAAGATGAGGACTTCAAGCAGTATATCGGCCATCAGACTAGGTGGGAAATTCCGATGGTCGGCGAGCCGGAGCTGGCCAACGCGAAGGTTGGGGACATAGTGCAGCTGCAGCGGCGCGGGTTCTTCCGCGTGGACGTGGCCAGCGCGCCCCCCTCCCCGCACACCAGCCGCCCCGCCCCGCTGCTGCTGTTCCACGTTCCCGACGGACACACTAAAGAAATGCCCGGACAGTCACGTATCAAGGACTGA

Protein

MMRVSCNRSNPPLGGLMAVEFYRSSAKEIDIVWENDSVIILPNSTKPVPFGTSNDLIRILENTFNKSVSPLEKVTMNHWLSFSLILEDEIPKSVEYLDKTLGPLTYLVGETLSVSDFAVFSVLYVSSKFKEISKVIPSQNIRRWMKLVEAQPQVQTAIKALPSDALDKLTKVSSRKSPPTNAESGGRQQEGKFIELPHAEMGKVVVRFPPEASGYLHIGHAKAALLNQYYQQAFHGKLVMRFDDTNPAKENADFEKVILEDVAMLEIKPDMFTHTSQYFDLMMEYCEKLIRENKAFVDDTPAEQMKTEREQKIESKNRNNSVERNLQLWEEMKKGSNIGVQCCVRAKIDMQSANGCLRDPTIYRCKPEEHPRTGTQYKVYPTYDFACPIVDSIEGVTHVLRTMEYHDRDPQFYWFIEALGIRKPYIWEYSRLSMTNTVLSKRKLTWFVEQGLVDGWDDPRMPTVRGVLRRGMTVEALRQFIHAQGSSRSVVFMEWDKIWAINKKVIDPVAPRYTALETNPVPVNLKGVTSDSSLTVPLHPKNPDVGTKTIWVAPRLLIDQADAKTLKEGENATFINYGNVTIDKIHRSKDGTVTSIDGTPNLDNKDYKKTVKLTWLAESKQSPMVETYCVYFDHIISKAVLGKDEDFKQYIGHQTRWEIPMVGEPELANAKVGDIVQLQRRGFFRVDVASAPPSPHTSRPAPLLLFHVPDGHTKEMPGQSRIKD

Description

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.

Catalytic Activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)

Subunit

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.

Similarity

Belongs to the class-I aminoacyl-tRNA synthetase family.
In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords

Alternative splicing   Aminoacyl-tRNA synthetase   ATP-binding   Complete proteome   Ligase   Metal-binding   Multifunctional enzyme   Nucleotide-binding   Phosphoprotein   Protein biosynthesis   Reference proteome   Repeat   RNA-binding   Zinc  

Feature

chain  Bifunctional glutamate/proline--tRNA ligase
splice variant  In isoform B.

Uniprot

H9J6H4   A0A2H1WFZ5   A0A2A4J483   A0A2A4J4S7   A0A194Q932   A0A336L220   + More
A0A336L852   A0A139WFG4   A0A1Q3F221   A0A182NG17   A0A182Q3N8   A0A182SQU0   A0A182WRS5   A0A1J1IVI3   A0A1Y1M9C0   A0A182M1F8   A0A182XWB2   Q7PRA2   Q178Q6   A0A182LG26   A0A182UM46   A0A182UC14   A0A182HU04   A0A084WR59   A0A3F2YU35   B4M0G6   B4K6U5   A0A182RND0   A0A182FL30   A0A182P6L4   A0A182IPC6   A0A0M3QYL1   B3M314   W5J719   B4JT86   A0A2M4A0W7   A0A2M4A119   A0A3B0KGI1   A0A2M4B8P7   A0A1B0FQR2   A0A1B6LQ16   A0A1I8MW01   B0XC16   A0A1A9UX28   B4G4B7   B4N9E6   A0A0L0C9X6   A0A1A9WPS7   W4VRQ8   A0A034VDY8   Q298M5   B4PL45   P28668   A0A1W4U806   A0A2J7R2T7   A0A0A1WW79   W8C9A2   B4HG12   B4QSD7   A0A1I8PHD6   A0A1A9ZK45   A0A1B0BXM6   A0A1B6EWL2   A0A1A9YLA9   B3P8B5   A0A1L8DRV9   A0A1B0CAF5   A0A146LEC7   A0A182VYL6   A0A1B6CRJ3   T1IPR4   N6TI36   A0A0P5JVV4   A0A0P5SAH8   A0A0N8E716   A0A0P6F0A3   A0A0P5KC80   A0A0P5G3D5   A0A0P5HW32   A0A0N8B0P1   A0A0P5JFC7   A0A0P6EYY1   A0A0P5I379   A0A0P5XHC9   A0A0P5HLU0   A0A0N7ZJZ0   A0A0P6EMB3   A0A0P5HQR8   A0A0P6HVR4   A0A0P6JTC3   A0A0P5Q9R7   A0A0P5IB91   A0A0P6DN03   A0A0P5I0B7   A0A0P5RT24   A0A0P5QA20   A0A0P5PBN8   A0A0P5KNW5   A0A0N8AHZ5   A0A0P5ABU0  

Pubmed

19121390    26354079    18362917    19820115    28004739    25244985    + More
12364791    14747013    17210077    17510324    20966253    24438588    17994087    20920257    23761445    25315136    26108605    25348373    15632085    17550304    1756734    9063462    10731132    12537572    12537569    18327897    25830018    24495485    26823975    23537049   

EMBL

BABH01019736    BABH01019737    ODYU01008408    SOQ51981.1    NWSH01003255    PCG66679.1    + More
PCG66678.1    KQ459324    KPJ01475.1    UFQS01001447    UFQT01001447    SSX10927.1    SSX30605.1    UFQS01002076    UFQT01002076    SSX13151.1    SSX32590.1    KQ971352    KYB26720.1    GFDL01013440    JAV21605.1    AXCN02001200    CVRI01000061    CRL04183.1    GEZM01041270    JAV80536.1    AXCM01001293    AAAB01008859    EAA07591.6    CH477360    EAT42685.1    ATLV01025930    ATLV01025931    ATLV01025932    KE525402    KFB52703.1    CH940650    EDW68345.2    CH933806    EDW14211.1    CP012526    ALC47809.1    CH902617    EDV42414.1    ADMH02002130    ETN58579.1    CH916373    EDV94976.1    GGFK01001071    MBW34392.1    GGFK01001099    MBW34420.1    OUUW01000007    SPP82758.1    GGFJ01000230    MBW49371.1    CCAG010019255    GEBQ01014199    JAT25778.1    DS232669    EDS44579.1    CH479179    EDW24465.1    CH964232    EDW80579.1    JRES01000809    KNC28249.1    GANO01000693    JAB59178.1    GAKP01019204    JAC39748.1    CM000070    EAL27930.1    CM000160    EDW99030.2    M74104    U59923    AE014297    AY058703    BT001645    NEVH01007826    PNF35140.1    GBXI01011421    JAD02871.1    GAMC01006822    GAMC01006821    JAB99734.1    CH480815    EDW43405.1    CM000364    EDX14136.1    JXJN01022279    GECZ01027410    JAS42359.1    CH954182    EDV53939.1    GFDF01004868    JAV09216.1    AJWK01003746    GDHC01011976    JAQ06653.1    GEDC01030360    GEDC01021260    JAS06938.1    JAS16038.1    JH431265    APGK01036917    KB740945    ENN77483.1    GDIQ01192741    JAK58984.1    GDIQ01094567    JAL57159.1    GDIQ01055540    JAN39197.1    GDIQ01055539    JAN39198.1    GDIQ01186185    JAK65540.1    GDIQ01247069    JAK04656.1    GDIQ01224334    JAK27391.1    GDIQ01224331    JAK27394.1    GDIQ01225534    JAK26191.1    GDIQ01055541    JAN39196.1    GDIQ01224332    JAK27393.1    GDIP01084425    JAM19290.1    GDIQ01225535    JAK26190.1    GDIP01238017    JAI85384.1    GDIQ01060535    JAN34202.1    GDIQ01224333    JAK27392.1    GDIQ01012947    JAN81790.1    GDIQ01009209    JAN85528.1    GDIQ01117221    JAL34505.1    GDIQ01220236    JAK31489.1    GDIQ01075183    JAN19554.1    GDIQ01220237    JAK31488.1    GDIQ01114768    JAL36958.1    GDIQ01117222    JAL34504.1    GDIQ01130863    JAL20863.1    GDIQ01188693    JAK63032.1    GDIP01145577    JAJ77825.1    GDIP01201415    JAJ21987.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000007266    UP000075884    UP000075886    + More
UP000075901    UP000076407    UP000183832    UP000075883    UP000076408    UP000007062    UP000008820    UP000075882    UP000075903    UP000075902    UP000030765    UP000075881    UP000008792    UP000009192    UP000075900    UP000069272    UP000075885    UP000075880    UP000092553    UP000007801    UP000000673    UP000001070    UP000268350    UP000092444    UP000095301    UP000002320    UP000078200    UP000008744    UP000007798    UP000037069    UP000091820    UP000001819    UP000002282    UP000000803    UP000192221    UP000235965    UP000001292    UP000000304    UP000095300    UP000092445    UP000092460    UP000092443    UP000008711    UP000092461    UP000075920    UP000019118   

PRIDE

H9J6H4    P28668   

Pfam

PF00749   tRNA-synt_1c        + More
PF03950   tRNA-synt_1c_C
PF14497   GST_C_3
PF09180   ProRS-C_1
PF00587   tRNA-synt_2b
PF00458   WHEP-TRS
PF03129   HGTP_anticodon
PF02463   SMC_N
PF00043   GST_C

Interpro

IPR020059   Glu/Gln-tRNA-synth_Ib_codon-bd        + More
IPR004046   GST_C       
IPR036282   Glutathione-S-Trfase_C_sf       
IPR004526   Glu-tRNA-synth_arc/euk       
IPR001412   aa-tRNA-synth_I_CS       
IPR011035   Ribosomal_L25/Gln-tRNA_synth       
IPR010987   Glutathione-S-Trfase_C-like       
IPR020058   Glu/Gln-tRNA-synth_Ib_cat-dom       
IPR020056   Rbsml_L25/Gln-tRNA_synth_N       
IPR014729   Rossmann-like_a/b/a_fold       
IPR000924   Glu/Gln-tRNA-synth       
IPR004154   Anticodon-bd       
IPR017449   Pro-tRNA_synth_II       
IPR016061   Pro-tRNA_ligase_II_C       
IPR006195   aa-tRNA-synth_II       
IPR002314   aa-tRNA-synt_IIb       
IPR009068   S15_NS1_RNA-bd       
IPR000738   WHEP-TRS_dom       
IPR004499   Pro-tRNA-ligase_IIa_arc-type       
IPR033721   ProRS_core_arch_euk       
IPR036621   Anticodon-bd_dom_sf       
IPR003395   RecF/RecN/SMC_N       
IPR027417   P-loop_NTPase       
IPR036277   SMC_hinge_sf       
IPR020061   Glu_tRNA_lig_a-bdl       

SUPFAM

SSF47616   SSF47616        + More
SSF50715   SSF50715       
SSF47060   SSF47060       
SSF64586   SSF64586       
SSF52540   SSF52540       
SSF75553   SSF75553       

Gene 3D

2.40.240.10     3.40.50.620     3.30.110.30     3.40.50.800     1.10.1160.10    

CDD

cd00778  ProRS_core_arch_euk

ProteinModelPortal

H9J6H4   A0A2H1WFZ5   A0A2A4J483   A0A2A4J4S7   A0A194Q932   A0A336L220   + More
A0A336L852   A0A139WFG4   A0A1Q3F221   A0A182NG17   A0A182Q3N8   A0A182SQU0   A0A182WRS5   A0A1J1IVI3   A0A1Y1M9C0   A0A182M1F8   A0A182XWB2   Q7PRA2   Q178Q6   A0A182LG26   A0A182UM46   A0A182UC14   A0A182HU04   A0A084WR59   A0A3F2YU35   B4M0G6   B4K6U5   A0A182RND0   A0A182FL30   A0A182P6L4   A0A182IPC6   A0A0M3QYL1   B3M314   W5J719   B4JT86   A0A2M4A0W7   A0A2M4A119   A0A3B0KGI1   A0A2M4B8P7   A0A1B0FQR2   A0A1B6LQ16   A0A1I8MW01   B0XC16   A0A1A9UX28   B4G4B7   B4N9E6   A0A0L0C9X6   A0A1A9WPS7   W4VRQ8   A0A034VDY8   Q298M5   B4PL45   P28668   A0A1W4U806   A0A2J7R2T7   A0A0A1WW79   W8C9A2   B4HG12   B4QSD7   A0A1I8PHD6   A0A1A9ZK45   A0A1B0BXM6   A0A1B6EWL2   A0A1A9YLA9   B3P8B5   A0A1L8DRV9   A0A1B0CAF5   A0A146LEC7   A0A182VYL6   A0A1B6CRJ3   T1IPR4   N6TI36   A0A0P5JVV4   A0A0P5SAH8   A0A0N8E716   A0A0P6F0A3   A0A0P5KC80   A0A0P5G3D5   A0A0P5HW32   A0A0N8B0P1   A0A0P5JFC7   A0A0P6EYY1   A0A0P5I379   A0A0P5XHC9   A0A0P5HLU0   A0A0N7ZJZ0   A0A0P6EMB3   A0A0P5HQR8   A0A0P6HVR4   A0A0P6JTC3   A0A0P5Q9R7   A0A0P5IB91   A0A0P6DN03   A0A0P5I0B7   A0A0P5RT24   A0A0P5QA20   A0A0P5PBN8   A0A0P5KNW5   A0A0N8AHZ5   A0A0P5ABU0  

PDB

2RE8     E-value=2.77666e-72,     Score=694

KEGG

101739247    K14163 bifunctional glutamyl/prolyl-tRNA synthetase [EC:6.1.1.17 6.1.1.15] | (RefSeq) bifunctional g

PATHWAY

00860    Porphyrin and chlorophyll metabolism - Bombyx mori (domestic silkworm)
00970    Aminoacyl-tRNA biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0005737   GO:0006424   GO:0005524   GO:0004818   GO:0004827   GO:0006433   GO:0004819   GO:0006425   GO:0005829   GO:0005694   GO:0051276   GO:0017101   GO:0003723   GO:0046872   GO:0000166   GO:0004812   GO:0006418   GO:0006412   GO:0016876   GO:0043039  

Length:

724

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.520409999999999

Exp number, first 60 AAs:

0.000580000000000001

Total prob of N-in:

0.02432

outside

1  -  724

 

 

Pi

196.172237

Theta

167.691628

Tajima's D

1.86725

CLR

0.224376

CSRT

0.860806959652017

Interpretation

Uncertain