Gene

KWMTBOMO15357  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA005117

Annotation

PREDICTED:_bifunctional_glutamate/proline--tRNA_ligase_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr25(-):10017982-10040186      View in EpiBrowser

Full name

Bifunctional glutamate/proline--tRNA ligase      

Alternative Name

Bifunctional aminoacyl-tRNA synthetase

Location in the cell

Cytoplasmic   Reliability : 1.713 Mitochondrial   Reliability : 1.035

CDS

ATGTTATCGCAAATTGGAACGGAATTATTGATTACGCCGAAACCAGCTCAGAGCGTTCAAGCGAGCAAGCCGGTGGCCGCGGCGCTGACTCCAACCTCGTCAGATTTGAACGACCAGATCACAAAACAGGGAGATCTGGTGAGATCTCTCAAAGCTGCTAAAGCCGAAAAGACAAAAGTTGACGAAGCTGTCAAAGTGCTTTTAGAATTAAAGGCTAAATATAAAACGGCCACGGGACAAGACTGGAAGCCAGGTACCGCAGCACCAGCACCAGCACATTCCGCATCTCCCTCCGGTGACGCAGCGTCGCTAGACCAGCAGATCACGAAGCAAGGCGACTTAGTTCGTTCCCTGAAAACGTCTAAGGCCGAAAAATCGAAAATTGACGCGGCTGTCAAGGCATTGCTGGAACTCAAAGCCAAATACAAGGCTGCCACCGGACAAGATTGGAAGCCAGGATCCGCGCCTGCAGCAATGGCCACCCCCGCACCGTCCAATGACGTCACCGCTCTCGATCGTGACGTCACCGCGCAGGGAGACTTGGTGCGCTCGTTGAAAGCGTCTAAAGCCGACAAGGCCAAGATCGACGAGGCCGTCAAGCAACTCCTGGACTTGAAAGCGAAGTACAAGGCTGCCGCTGGACAAGACTGGAAACCGGGAGCGAAACCAGCGCCGCCGCAGCAAGCTGCTCCGCCGGCGGCCGATCCGAACGTAGCGGAACTCGCGTCGCAAATCGAAGCCCAAGGTGACAAAGTTCGGAGGATCAAAAGCGAAAAAGCCGATAAATCAGTCGTCGACGTCGAGGTCAAAAATCTGTTGAACCTCAAAGCTCAATACAAAGCAATGACTGGAAGCGACTGGACACCGAAACCTAATCAAGCGAAAAGCAATCAGGCGAGCGCTAAACCGACCCCGGCTGCTAAGGTACCGAAGACGCAACCACCTAAACCTGTAAAGGAGGAATCTTCAAGCGGAGTCAAGAAGGTCACGAGACTAGGGCTGGAGGCCAGCAAGGAGACCGATCTATCCGAATGGTATTCGCAAGTCATCACGAAATCGGAAATGATCGACTACTACGACATATCTGGTTGCTACATACTGCGGCCGTGGTCGTTCAGTATATGGGACACGATAAGGCACTTCCTCAGTGCCGAATTCCGGAAGCTCGGAGTCAAAGACGGATATTTTCCTATATTCGTATCGAAGGCGGCGCTAGAGCGAGAGAAGACGCACATAGCTGACTTCGCGCCGGAAGTGGCGTGGGTGACGCACTCGGGCTCGTCCGAGCTCGCCGAGCCCATCGCCGTGCGCCCCACCTCCGAGACCGTCATGTACCCCGCCTACGCCAAGTGGATCCAGAGCCACAGGGACCTGCCGCTCAAACTCAACCAGTGGAACAACGTCGTTAGATGGGAGTTCAAGCAACCGCAACCTTTCCTGAGGACGCGCGAGTTCCTCTGGCAGGAAGGACACACCGCTTTCCGGACCAAAGAGGAGGCGGCCGAAGAGGTCTTAATTATATTAGATTTATATGCTCGCGTATACGAAGAGTTGCTAGCGATACCGGTGATCAAAGGTCGTAAAACAGAGAAGGAGAAGTTTGCTGGCGGCGACTACACCACCACGGTCGAAGCCTACGTACCGGCCAGCGGCAGAGGCGTTCAGGGAGCGACGAGTCATCACCTAGGTCAAAACTTTTCGAAGATGTTCGAGATTGTATACGACGATGCCGAAACTCTGGAGAAGAATTACGTCTATCAGAATTCGTGGGGCATCACAACTAGAACTATTGGTGTAATGGTGTTGGTTCACGGAGACGACAAGGGCCTAGTGCTGCCCCCTCGCGTCGCCGAGATACAGGCCGTGGTGGTTCCGTGCGGCATCACGGCGTCCAGCTCGGCGGACGAGCGCCAGGCGCTGATGGACTCCTGCAAGGCGCTAGTCAACGACCTGCTCGCCGCCGGAGTGAGGGCCGAAGGCGACTACAGGGATAACTATTCGCCTGGCTGGAAATTTAACCACTGGGAGTTGAAGGGGGTTCCAGTAAGAGTTGAATTAGGTCCCAAGGATATAGCAAAGGGTACAGTTGTAGCAGTACAGCGTCTCACCGGTGATAAGATGGTCTTCAAACGTGATTCTGTTGCAAAAGACATTGTTGAACTCCTTGATAAAACACACGCGCAAATGTTTGAAAGGGCAACGAAAGATCGAGATGCTCGGTTATCATCTGTTACTAAATGGGAAGATTTCACAGCTGCTCTTGAAGAAAAAAACATTTTACTTGCACCATTTTGTGGAGAGATATCGTGTGAAGATAACATTAAGAACGACAGTGCAAGGACAGAAGACGACCCTAACGTTGATGTCAAGGCACCAGCCATGGGTGCGAAATCACTGTGCATACCATTTGAACCTCCAAGACAATTATCTGAATCTGACCGCTGCATACACCCGGCTTGTAAAAATAAGCCCAAGTTCATTACATTATTTGGAAGAAGTTACTGA

Protein

MLSQIGTELLITPKPAQSVQASKPVAAALTPTSSDLNDQITKQGDLVRSLKAAKAEKTKVDEAVKVLLELKAKYKTATGQDWKPGTAAPAPAHSASPSGDAASLDQQITKQGDLVRSLKTSKAEKSKIDAAVKALLELKAKYKAATGQDWKPGSAPAAMATPAPSNDVTALDRDVTAQGDLVRSLKASKADKAKIDEAVKQLLDLKAKYKAAAGQDWKPGAKPAPPQQAAPPAADPNVAELASQIEAQGDKVRRIKSEKADKSVVDVEVKNLLNLKAQYKAMTGSDWTPKPNQAKSNQASAKPTPAAKVPKTQPPKPVKEESSSGVKKVTRLGLEASKETDLSEWYSQVITKSEMIDYYDISGCYILRPWSFSIWDTIRHFLSAEFRKLGVKDGYFPIFVSKAALEREKTHIADFAPEVAWVTHSGSSELAEPIAVRPTSETVMYPAYAKWIQSHRDLPLKLNQWNNVVRWEFKQPQPFLRTREFLWQEGHTAFRTKEEAAEEVLIILDLYARVYEELLAIPVIKGRKTEKEKFAGGDYTTTVEAYVPASGRGVQGATSHHLGQNFSKMFEIVYDDAETLEKNYVYQNSWGITTRTIGVMVLVHGDDKGLVLPPRVAEIQAVVVPCGITASSSADERQALMDSCKALVNDLLAAGVRAEGDYRDNYSPGWKFNHWELKGVPVRVELGPKDIAKGTVVAVQRLTGDKMVFKRDSVAKDIVELLDKTHAQMFERATKDRDARLSSVTKWEDFTAALEEKNILLAPFCGEISCEDNIKNDSARTEDDPNVDVKAPAMGAKSLCIPFEPPRQLSESDRCIHPACKNKPKFITLFGRSY

Description

Catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA.

Catalytic Activity

ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)

Subunit

Component of the multisynthetase complex which is comprised of a bifunctional glutamyl-prolyl-tRNA synthetase, the monospecific isoleucyl, leucyl, glutaminyl, methionyl, lysyl, arginyl, and aspartyl-tRNA synthetases as well as three auxiliary proteins, p18, p48 and p43.

Similarity

In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.
In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords

Alternative splicing   Aminoacyl-tRNA synthetase   ATP-binding   Complete proteome   Ligase   Metal-binding   Multifunctional enzyme   Nucleotide-binding   Phosphoprotein   Protein biosynthesis   Reference proteome   Repeat   RNA-binding   Zinc  

Feature

chain  Bifunctional glutamate/proline--tRNA ligase
splice variant  In isoform B.

Uniprot

H9J6H5   A0A2H1WFZ5   A0A2A4J4S7   A0A2A4J483   A0A212FJ95   A0A182P6L4   + More
A0A336L220   A0A3B0KGI1   A0A3F2YU35   A0A182RND0   A0A3B0K9L2   P28668-2   H8F4R5   A0A0R1E9D1   B3P8B5   A0A1W4U806   A0A336L852   Q178Q6   B4PL45   A0A1J1IVI3   A0A182M1F8   P28668   T1PD31   A0A0M3QYL1   A0A182HU04   A0A1I8MW01   B0XC16   A0A182H987   B4G4B7   A0A182IPC6   A0A182NG17   Q298M5   Q7PRA2   A0A1Q3F221   A0A182LG26   B4K6U5   A0A182WRS5   A0A0K8SGP1   A0A084WR59   A0A182VYL6   A0A0L0C9X6   A0A2M4B932   A0A182UM46   A0A182FL30   A0A182XWB2   W8C9A2   W5J719   A0A146LEC7   A0A2M4CPC0   A0A2M4B8P7   B4M0G6   A0A2M4A0W7   A0A2M4A119   B4JT86   A0A034VDY8   A0A182Q3N8   A0A139WFQ4   B4N9E6   A0A1I8PHD6   A0A0A1XP37   A0A0K8U4D3   A0A1A9UX28   A0A0A1WW79   A0A232EXF6   A0A1L8DRV9   K1QNW9   A0A1B0BXM6   W4VRQ8   A0A1A9YLA9   A0A1A9ZK45   A0A0N7ZML5   A0A0P5VRJ7   A0A0P5D0H6   A0A0N7ZJ34   A0A0P6FU54   A0A0P5BRK0   A0A1B0GML6   A0A0P5C6J9   A0A1Y1M9C0   A0A0P5XJQ5   A0A0P5BXI0   A0A0P5DHR7   A0A0P5RT24   A0A0N8CVS1   A0A0P5EJI7   A0A0P5ABU0   E9H862   A0A0P5TB53   A0A0P5ULW1   A0A0P5CRD7   A0A0P5YNQ6   A0A0P6HJS2   A0A0P5TLX8   A0A0P5ZGX6   A0A0P4ZG37   A0A1B0CAF5   A0A0N8AHZ5   C3XZ88   A0A0P4Z6F2  

Pubmed

19121390    22118469    1756734    9063462    10731132    12537572    + More
12537569    18327897    17994087    17550304    17510324    25315136    26483478    15632085    12364791    14747013    17210077    20966253    24438588    26108605    25244985    24495485    20920257    23761445    26823975    25348373    18362917    19820115    25830018    28648823    22992520    28004739    21292972    18563158   

EMBL

BABH01019740    BABH01019741    ODYU01008408    SOQ51981.1    NWSH01003255    PCG66678.1    + More
PCG66679.1    AGBW02008295    OWR53802.1    UFQS01001447    UFQT01001447    SSX10927.1    SSX30605.1    OUUW01000007    SPP82758.1    SPP82759.1    M74104    U59923    AE014297    AY058703    BT001645    BT133305    AFD10752.1    CM000160    KRK04627.1    CH954182    EDV53939.1    UFQS01002076    UFQT01002076    SSX13151.1    SSX32590.1    CH477360    EAT42685.1    EDW99030.2    CVRI01000061    CRL04183.1    AXCM01001293    KA646015    AFP60644.1    CP012526    ALC47809.1    DS232669    EDS44579.1    JXUM01030070    JXUM01030071    KQ560872    KXJ80532.1    CH479179    EDW24465.1    CM000070    EAL27930.1    AAAB01008859    EAA07591.6    GFDL01013440    JAV21605.1    CH933806    EDW14211.1    GBRD01013876    JAG51950.1    ATLV01025930    ATLV01025931    ATLV01025932    KE525402    KFB52703.1    JRES01000809    KNC28249.1    GGFJ01000371    MBW49512.1    GAMC01006822    GAMC01006821    JAB99734.1    ADMH02002130    ETN58579.1    GDHC01011976    JAQ06653.1    GGFL01002937    MBW67115.1    GGFJ01000230    MBW49371.1    CH940650    EDW68345.2    GGFK01001071    MBW34392.1    GGFK01001099    MBW34420.1    CH916373    EDV94976.1    GAKP01019204    JAC39748.1    AXCN02001200    KQ971352    KYB26719.1    CH964232    EDW80579.1    GBXI01001168    JAD13124.1    GDHF01030717    JAI21597.1    GBXI01011421    JAD02871.1    NNAY01001764    OXU22980.1    GFDF01004868    JAV09216.1    JH819095    EKC32794.1    JXJN01022279    GANO01000693    JAB59178.1    GDIP01230617    JAI92784.1    GDIP01096380    JAM07335.1    GDIP01164565    JAJ58837.1    GDIP01240465    JAI82936.1    GDIQ01053741    JAN40996.1    GDIP01181019    JAJ42383.1    AJVK01026286    AJVK01026287    AJVK01026288    GDIP01179067    JAJ44335.1    GEZM01041270    JAV80536.1    GDIP01071133    JAM32582.1    GDIP01179066    JAJ44336.1    GDIP01156116    JAJ67286.1    GDIQ01114768    JAL36958.1    GDIP01094079    JAM09636.1    GDIP01145576    JAJ77826.1    GDIP01201415    JAJ21987.1    GL732603    EFX72016.1    GDIP01132669    GDIP01129868    JAL71045.1    GDIP01147077    GDIP01111088    JAL92626.1    GDIP01166563    JAJ56839.1    GDIP01056080    JAM47635.1    GDIQ01041593    JAN53144.1    GDIP01124824    GDIP01044041    LRGB01000005    JAL78890.1    KZS22036.1    GDIP01218962    GDIP01084424    GDIP01045486    JAM58229.1    GDIP01217262    JAJ06140.1    AJWK01003746    GDIP01145577    JAJ77825.1    GG666475    EEN66652.1    GDIP01217261    JAJ06141.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000075885    UP000268350    UP000075881    + More
UP000075900    UP000000803    UP000002282    UP000008711    UP000192221    UP000008820    UP000183832    UP000075883    UP000092553    UP000095301    UP000002320    UP000069940    UP000249989    UP000008744    UP000075880    UP000075884    UP000001819    UP000007062    UP000075882    UP000009192    UP000076407    UP000030765    UP000075920    UP000037069    UP000075903    UP000069272    UP000076408    UP000000673    UP000008792    UP000001070    UP000075886    UP000007266    UP000007798    UP000095300    UP000078200    UP000215335    UP000005408    UP000092460    UP000092443    UP000092445    UP000092462    UP000000305    UP000076858    UP000092461    UP000001554   

PRIDE

H9J6H5    P28668    H8F4R5   

Pfam

PF09180   ProRS-C_1        + More
PF03129   HGTP_anticodon
PF00458   WHEP-TRS
PF00587   tRNA-synt_2b
PF14497   GST_C_3
PF00749   tRNA-synt_1c
PF03950   tRNA-synt_1c_C
PF02463   SMC_N
PF00043   GST_C

Interpro

IPR006195   aa-tRNA-synth_II        + More
IPR016061   Pro-tRNA_ligase_II_C       
IPR017449   Pro-tRNA_synth_II       
IPR004154   Anticodon-bd       
IPR036621   Anticodon-bd_dom_sf       
IPR002316   Pro-tRNA-ligase_IIa       
IPR033721   ProRS_core_arch_euk       
IPR004499   Pro-tRNA-ligase_IIa_arc-type       
IPR009068   S15_NS1_RNA-bd       
IPR000738   WHEP-TRS_dom       
IPR002314   aa-tRNA-synt_IIb       
IPR036282   Glutathione-S-Trfase_C_sf       
IPR004046   GST_C       
IPR020059   Glu/Gln-tRNA-synth_Ib_codon-bd       
IPR020056   Rbsml_L25/Gln-tRNA_synth_N       
IPR014729   Rossmann-like_a/b/a_fold       
IPR000924   Glu/Gln-tRNA-synth       
IPR001412   aa-tRNA-synth_I_CS       
IPR004526   Glu-tRNA-synth_arc/euk       
IPR011035   Ribosomal_L25/Gln-tRNA_synth       
IPR020058   Glu/Gln-tRNA-synth_Ib_cat-dom       
IPR010987   Glutathione-S-Trfase_C-like       
IPR003395   RecF/RecN/SMC_N       
IPR036277   SMC_hinge_sf       
IPR027417   P-loop_NTPase       
IPR020061   Glu_tRNA_lig_a-bdl       

SUPFAM

SSF64586   SSF64586        + More
SSF47060   SSF47060       
SSF50715   SSF50715       
SSF47616   SSF47616       
SSF52540   SSF52540       
SSF75553   SSF75553       

Gene 3D

3.30.110.30     3.40.50.800     2.40.240.10     3.40.50.620     1.10.1160.10    

CDD

cd00778  ProRS_core_arch_euk

ProteinModelPortal

H9J6H5   A0A2H1WFZ5   A0A2A4J4S7   A0A2A4J483   A0A212FJ95   A0A182P6L4   + More
A0A336L220   A0A3B0KGI1   A0A3F2YU35   A0A182RND0   A0A3B0K9L2   P28668-2   H8F4R5   A0A0R1E9D1   B3P8B5   A0A1W4U806   A0A336L852   Q178Q6   B4PL45   A0A1J1IVI3   A0A182M1F8   P28668   T1PD31   A0A0M3QYL1   A0A182HU04   A0A1I8MW01   B0XC16   A0A182H987   B4G4B7   A0A182IPC6   A0A182NG17   Q298M5   Q7PRA2   A0A1Q3F221   A0A182LG26   B4K6U5   A0A182WRS5   A0A0K8SGP1   A0A084WR59   A0A182VYL6   A0A0L0C9X6   A0A2M4B932   A0A182UM46   A0A182FL30   A0A182XWB2   W8C9A2   W5J719   A0A146LEC7   A0A2M4CPC0   A0A2M4B8P7   B4M0G6   A0A2M4A0W7   A0A2M4A119   B4JT86   A0A034VDY8   A0A182Q3N8   A0A139WFQ4   B4N9E6   A0A1I8PHD6   A0A0A1XP37   A0A0K8U4D3   A0A1A9UX28   A0A0A1WW79   A0A232EXF6   A0A1L8DRV9   K1QNW9   A0A1B0BXM6   W4VRQ8   A0A1A9YLA9   A0A1A9ZK45   A0A0N7ZML5   A0A0P5VRJ7   A0A0P5D0H6   A0A0N7ZJ34   A0A0P6FU54   A0A0P5BRK0   A0A1B0GML6   A0A0P5C6J9   A0A1Y1M9C0   A0A0P5XJQ5   A0A0P5BXI0   A0A0P5DHR7   A0A0P5RT24   A0A0N8CVS1   A0A0P5EJI7   A0A0P5ABU0   E9H862   A0A0P5TB53   A0A0P5ULW1   A0A0P5CRD7   A0A0P5YNQ6   A0A0P6HJS2   A0A0P5TLX8   A0A0P5ZGX6   A0A0P4ZG37   A0A1B0CAF5   A0A0N8AHZ5   C3XZ88   A0A0P4Z6F2  

PDB

4K88     E-value=0,     Score=1786

KEGG

101739247    K14163 bifunctional glutamyl/prolyl-tRNA synthetase [EC:6.1.1.17 6.1.1.15] | (RefSeq) bifunctional g

GO

GO:0005524   GO:0006433   GO:0004827   GO:0005737   GO:0006424   GO:0004818   GO:0051276   GO:0005694   GO:0046872   GO:0003723   GO:0017101   GO:0004812   GO:0006418   GO:0000166   GO:0004222   GO:0031012   GO:0008233  

Length:

834

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00019

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00001

outside

1  -  834

 

 

Pi

229.39846

Theta

184.051021

Tajima's D

0.903518

CLR

0.18704

CSRT

0.631968401579921

Interpretation

Uncertain