Gene

KWMTBOMO15293  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA005151

Annotation

PREDICTED:_DNA_replication_licensing_factor_mcm5_[Papilio_polytes]

Location

Bomo_Chr25(-):8478876-8486842      View in EpiBrowser

Full name

DNA helicase       + More
DNA replication licensing factor Mcm5      
DNA replication licensing factor mcm5      
DNA replication licensing factor mcm5-A      
DNA replication licensing factor mcm5-B      

Alternative Name

Minichromosome maintenance 5 protein
CDC46 homolog A
CDC46p
p92
CDC46 homolog B

Location in the cell

Mitochondrial   Reliability : 1.274 Nuclear   Reliability : 1.717

CDS

ATGGAAGGTTTTGATGATCCTGGTGTATTTTTCTCTGATAATTTTGGTGTAGAAGAAAACGAATCTCAAGATCATGTTAATTTACAAGCAGTCAAAAAGAAATTCAAAGAATTCATGAGACAGTTTCATACTGGAAACTTCAATTACAAATACAGAGATGCCCTCAAACGCAACTATAACCTAAACCAATATTGGGTCGAGATAAATATTGAAGATTTGTCTAGTTTTGATGACGTTCTTGCTGAAAAATTATACAAGAAACCAACTGAACATTTGCCTATACTAGAAGAAGCTGCTAAAGAGTTAGCTGATGAGTTGACTGCACCGAGACCAGAAGGTGAGGAGAAAGTTGAGGATATTCAAGTGCTTTTGTCATCAGATGCACATTCATCAAATTTGAGGGAGCTTAAATCAGAAACTGTATCAAGACTAGTGAAGATTCCTGGTATTGTAATATCAGCATCCGGTATTAAAGCAAAAGCCACAAAAATATCCATACAATGTCGCTCATGCCGCAATGTTATTCCCAATCTTCCTGTGAAGCCAGGTCTGGAAGGATATGTCATGCCAAGAAAATGCAATACAGAACAATCTGGACGTCCTAAATGTCCTTTAGATCCATATTTCATTATTCCCGACAAATGCAAATGTATCGATTATCAGGTGTTGAAACTTCAAGAAGCACCAGAAAGTATTCCACAAGGAGAAATGCCAAGGCATTTGACTGTATACTGTGATCGTACATTATGCGAACGTGTTGCACCAGGCGCACGAGTCACGGTTTTGGGGATTTATTCTATAAAAAAGATAGCAAAAGTAGGGCGCGAGGGTCGCGACAAAGGCTCCGTAGGCGTCCGGTCATCGTACCTACGAGCCGTAGGGCTCACCGCAGAGGAAGGTGTCACTAGCGGCCTGAGACCCTTTACTGCAGAAGAAGAAGAGCAATTCAGAAGACTCGCTGCCTCTCCAGACATTTATGAAAGGATCGGAAAGTCCATTGCTCCAAGTGTTTTTGGTGCTGTTGATATGAAGAAAGCTATCGCTTGTCTGCTTTTTGGAGGATCTAGAAAACGCCTACCGGATGGCCTGACTCGTAGAGGAGATATAAATATACTTTTACTTGGAGACCCTGGTACTGCCAAATCTCAATTGCTAAAATTTGTGGAGAAGGTTGCCCCGATTGGCGTTTATACCTCAGGGAAAGGTTCGAGTGCAGCAGGTCTCACCGCTTCAGTCATTAGAGATCCTGGAAGCCGTAACTTCGTAATGGAGGGAGGGGCTATGGTTTTGGCTGATGGTGGTGTTGTTTGTATAGACGAATTTGATAAGATGAGAGAAGATGATCGCGTCGCCATACACGAGGCAATGGAACAACAAACTATTTCTATTGCTAAGGCGGGTATCACCACTACATTAAATTCTCGTTGTTCTGTCCTGGCGGCCGCCAACTCTGTATTCGGACGATGGGACGATACTAAAGCTGATGATAACATTGATTTCATGCCAACAATTTTATCCAGATTCGACATGATTTTTATAGTCAAAGATGAACATGATCAGAACAGAGATATAACGCTCGCGAAGCACATAATCAGCGTGCACATGGGCGGAGAGAGCAGCGAGCGTGCGGCGGCGGCGGGCGAGCTGCCGCTGACGACGCTGCGTCGGTACAGCGCGTACTGCCGCGCGCGCTGCGGGCCCCGCCTGGCGCCGCCCGCCGCCGCCCGCCTGCAGGCGCGCTACGTGCTCATGCGGACCGGCGCGCTGCAACACGAGCGACAAGCCGACAAGCGTCTCTCCATACCCATCACCGTTAGGCAATTGGAAGCCATAGTTCGTATATCAGAATCTCTCGCTAAGATGCAGTTGCAACCGTTTGCGACTGAAGCTCACGTAAATGAAGCACTTCGTCTGTTCCAAGTGTCCACGCTAGATGCGGCTATGACTGGAAGTTTGGCAGGAGCTGAAGGTTTTACAACAGAAGAAGATCACGAAATGCTGACTCGCGTCGAGAAACAATTAAAGCGCAGGTTCGCTGTGGGCTCTCAGGTTTCTGAACAGACAATCATTCAAGACTTCTTGCGACAAAAATATCCAGAAAGAGCCATTCTTAAAGTAATACATATGATGATACGACGAGGGGAGCTTCAGCATAGATTACAGCGAAAAATGTTGTATAGACTCTCATAA

Protein

MEGFDDPGVFFSDNFGVEENESQDHVNLQAVKKKFKEFMRQFHTGNFNYKYRDALKRNYNLNQYWVEINIEDLSSFDDVLAEKLYKKPTEHLPILEEAAKELADELTAPRPEGEEKVEDIQVLLSSDAHSSNLRELKSETVSRLVKIPGIVISASGIKAKATKISIQCRSCRNVIPNLPVKPGLEGYVMPRKCNTEQSGRPKCPLDPYFIIPDKCKCIDYQVLKLQEAPESIPQGEMPRHLTVYCDRTLCERVAPGARVTVLGIYSIKKIAKVGREGRDKGSVGVRSSYLRAVGLTAEEGVTSGLRPFTAEEEEQFRRLAASPDIYERIGKSIAPSVFGAVDMKKAIACLLFGGSRKRLPDGLTRRGDINILLLGDPGTAKSQLLKFVEKVAPIGVYTSGKGSSAAGLTASVIRDPGSRNFVMEGGAMVLADGGVVCIDEFDKMREDDRVAIHEAMEQQTISIAKAGITTTLNSRCSVLAAANSVFGRWDDTKADDNIDFMPTILSRFDMIFIVKDEHDQNRDITLAKHIISVHMGGESSERAAAAGELPLTTLRRYSAYCRARCGPRLAPPAAARLQARYVLMRTGALQHERQADKRLSIPITVRQLEAIVRISESLAKMQLQPFATEAHVNEALRLFQVSTLDAAMTGSLAGAEGFTTEEDHEMLTRVEKQLKRRFAVGSQVSEQTIIQDFLRQKYPERAILKVIHMMIRRGELQHRLQRKMLYRLS

Description

Acts as component of the Mcm2-7 complex (Mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the Mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity.
Acts as component of the mcm2-7 complex (mcm complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the mcm2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity (By similarity).

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Subunit

Component of the Mcm2-7 complex. The complex forms a toroidal hexameric ring with the proposed subunit order Mcm2-Mcm6-Mcm4-Mcm7-Mcm3-Mcm5 (Probable).
Component of the mcm2-7 complex (RLF-M). The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (By simililarity).
Component of the mcm2-7 complex (RLF-M). The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5 (Probable). The heterodimer of mmcm3/mcm5 interacts with mcm4, mmcm6, mcm7 and weakly with mcm2.
Component of the mcm2-7 complex (RLF-M). The complex forms a toroidal hexameric ring with the proposed subunit order mcm2-mcm6-mcm4-mcm7-mcm3-mcm5. The heterodimer of mmcm3/mcm5 interacts with mcm4, mmcm6, mcm7 and weakly with mcm2 (By similarity).

Similarity

Belongs to the MCM family.

Keywords

ATP-binding   Cell cycle   Complete proteome   Cytoplasm   DNA replication   DNA-binding   Helicase   Hydrolase   Nucleotide-binding   Nucleus   Reference proteome   Alternative splicing  

Feature

chain  DNA replication licensing factor Mcm5
splice variant  In isoform 2.

Uniprot

A0A2H1W380   A0A2W1BKQ9   H9J6K9   A0A194PI49   A0A212F8Y1   A0A2P8Z223   + More
A0A2J7PYT0   A0A067QM81   A0A1B6D033   A0A0N0BDP3   A0A194RLA6   A0A3R7QW06   A0A154PHC8   A0A0J7NTI1   A0A2A3ERL1   A0A088A0G3   Q17H38   A0A182GQF3   A0A182FAF4   A0A026WH14   E2BWT0   E2A7N9   B0WNG2   W5JTL1   A0A182NCU9   A0A182PLH0   A0A089GK37   A0A151X597   A0A182M566   A0A182RI25   A0A158NAF4   A0A182W384   E9H6I5   A0A182VKP3   A0A084VDK0   A0A182XF67   A0A164K478   A0A1W4WBT4   A0A182Y908   A0A182K4W3   A0A1Q3EVG0   A0A182UHP0   A0A182KZR5   Q7QA70   A0A182HVN8   A0A182QVG1   A0A0P5V2S2   A0A0P6JBI5   A0A182JCU9   A0A0P5HSI7   A0A2P2HWJ7   A0A0C9RED7   A0A336L2Y3   A0A224YX91   A0A195CKR6   A0A0A1X8M6   A0A0C9S360   A0A131YXL0   L7M9Q6   A0A1Y1MI36   A0A0L7R1I7   W8BKX8   B3P1K0   B4QUP5   A0A034WVN9   B4HIQ0   Q9VGW6   B4PLD4   B3M291   A0A1E1X2S1   B4NJY3   B4KC98   A0A0L0BQ18   A0A1I8NCC4   A0A0P4W8T1   U5ELZ4   A0A0M5J5H1   A0A1W4W052   A0A1Z5L9M3   R7V2U4   A0A210Q0K1   A0A3B0JQH7   Q561P5   A0A1I8Q5I2   B4JHM4   A0A2R5LIX8   A0A293LWJ3   A0A1L8GNF3   P55862   K7J409   A0A099ZFS6   A0A091HGL6   A0A2I0USR1   Q5ZKL0   A0A232FEX0   A0A131XFY4   V5HPK2   A0A1L8GGS4   B4M5I3   Q6PCI7  

EC Number

3.6.4.12

Pubmed

28756777    19121390    26354079    22118469    29403074    24845553    + More
17510324    26483478    24508170    30249741    20798317    20920257    23761445    21347285    21292972    24438588    25244985    20966253    12364791    14747013    17210077    28797301    25830018    26131772    26830274    25576852    28004739    24495485    17994087    25348373    9224901    10731132    12537572    16798881    20122406    17550304    28503490    26108605    25315136    28528879    23254933    28812685    27762356    9214647    8917078    9214646    9851868    16369567    20075255    15592404    15642098    28648823    28049606    25765539   

EMBL

ODYU01006049    SOQ47560.1    KZ149994    PZC75478.1    BABH01019865    BABH01019866    + More
KQ459604    KPI92404.1    AGBW02009658    OWR50187.1    PYGN01000235    PSN50546.1    NEVH01020342    PNF21485.1    KK853179    KDR10221.1    GEDC01018365    JAS18933.1    KQ435863    KOX70391.1    KQ460045    KPJ18100.1    QCYY01001128    ROT80337.1    KQ434905    KZC11207.1    LBMM01001798    KMQ95730.1    KZ288194    PBC33912.1    CH477253    EAT45976.1    JXUM01080405    JXUM01094528    KQ564135    KQ563183    KXJ72707.1    KXJ74319.1    KK107231    QOIP01000009    EZA54966.1    RLU18576.1    GL451188    EFN79794.1    GL437365    EFN70560.1    DS232010    EDS31616.1    ADMH02000180    ETN67461.1    KM036513    AIP98400.1    KQ982519    KYQ55494.1    AXCM01000104    ADTU01010273    GL732597    EFX72666.1    ATLV01011556    KE524695    KFB36044.1    LRGB01003375    KZS02936.1    GFDL01015744    JAV19301.1    AAAB01008898    EAA09249.2    APCN01005808    AXCN02001206    GDIP01104936    GDIQ01019924    JAL98778.1    JAN74813.1    GDIQ01019925    JAN74812.1    GDIQ01229345    JAK22380.1    IACF01000398    LAB66175.1    GBYB01013200    GBYB01014969    JAG82967.1    JAG84736.1    UFQS01001768    UFQT01001768    SSX12278.1    SSX31729.1    GFPF01007296    MAA18442.1    KQ977642    KYN01047.1    GBXI01007202    JAD07090.1    GBZX01001246    JAG91494.1    GEDV01005342    JAP83215.1    GACK01005146    JAA59888.1    GEZM01036149    JAV82937.1    KQ414667    KOC64709.1    GAMC01008922    JAB97633.1    CH954181    EDV49599.1    CM000364    EDX13452.1    GAKP01001104    JAC57848.1    CH480815    EDW42697.1    U83493    AE014297    AY071628    CM000160    EDW96841.1    CH902617    EDV42282.1    GFAC01005623    JAT93565.1    CH964272    EDW83985.1    CH933806    EDW13707.1    JRES01001539    KNC22180.1    GDRN01063226    JAI64995.1    GANO01001217    JAB58654.1    CP012526    ALC47711.1    GFJQ02002864    JAW04106.1    AMQN01000770    KB295623    ELU12874.1    NEDP02005311    OWF42189.1    OUUW01000007    SPP83213.1    CR848584    BC093455    AAH93455.1    CAJ81496.1    CH916369    EDV93863.1    GGLE01005303    MBY09429.1    GFWV01007408    MAA32138.1    CM004472    OCT85375.1    U44048    D63920    BC047250    AAZX01001005    KL892383    KGL79685.1    KL217466    KFO95403.1    KZ505643    PKU49093.1    AC145933    AJ720074    CAG31733.1    NNAY01000320    OXU29242.1    GEFH01003960    JAP64621.1    GANP01006826    JAB77642.1    CM004473    OCT83020.1    CH940652    EDW58909.1    BC059310    AAH59310.1   

Proteomes

UP000005204    UP000053268    UP000007151    UP000245037    UP000235965    UP000027135    + More
UP000053105    UP000053240    UP000283509    UP000076502    UP000036403    UP000242457    UP000005203    UP000008820    UP000069940    UP000249989    UP000069272    UP000053097    UP000279307    UP000008237    UP000000311    UP000002320    UP000000673    UP000075884    UP000075885    UP000075809    UP000075883    UP000075900    UP000005205    UP000075920    UP000000305    UP000075903    UP000030765    UP000076407    UP000076858    UP000192223    UP000076408    UP000075881    UP000075902    UP000075882    UP000007062    UP000075840    UP000075886    UP000075880    UP000078542    UP000053825    UP000008711    UP000000304    UP000001292    UP000000803    UP000002282    UP000007801    UP000007798    UP000009192    UP000037069    UP000095301    UP000092553    UP000192221    UP000014760    UP000242188    UP000268350    UP000008143    UP000095300    UP000001070    UP000186698    UP000002358    UP000053641    UP000054308    UP000000539    UP000215335    UP000008792   

PRIDE

H9J6K9    Q9VGW6    V5HPK2   

Pfam

PF14551   MCM_N        + More
PF17855   MCM_lid
PF00493   MCM
PF17207   MCM_OB

Interpro

IPR012340   NA-bd_OB-fold        + More
IPR031327   MCM       
IPR001208   MCM_dom       
IPR041562   MCM_lid       
IPR033762   MCM_OB       
IPR008048   MCM5       
IPR027417   P-loop_NTPase       
IPR018525   MCM_CS       
IPR027925   MCM_N       
IPR003593   AAA+_ATPase       

SUPFAM

SSF52540   SSF52540        + More
SSF50249   SSF50249       

ProteinModelPortal

A0A2H1W380   A0A2W1BKQ9   H9J6K9   A0A194PI49   A0A212F8Y1   A0A2P8Z223   + More
A0A2J7PYT0   A0A067QM81   A0A1B6D033   A0A0N0BDP3   A0A194RLA6   A0A3R7QW06   A0A154PHC8   A0A0J7NTI1   A0A2A3ERL1   A0A088A0G3   Q17H38   A0A182GQF3   A0A182FAF4   A0A026WH14   E2BWT0   E2A7N9   B0WNG2   W5JTL1   A0A182NCU9   A0A182PLH0   A0A089GK37   A0A151X597   A0A182M566   A0A182RI25   A0A158NAF4   A0A182W384   E9H6I5   A0A182VKP3   A0A084VDK0   A0A182XF67   A0A164K478   A0A1W4WBT4   A0A182Y908   A0A182K4W3   A0A1Q3EVG0   A0A182UHP0   A0A182KZR5   Q7QA70   A0A182HVN8   A0A182QVG1   A0A0P5V2S2   A0A0P6JBI5   A0A182JCU9   A0A0P5HSI7   A0A2P2HWJ7   A0A0C9RED7   A0A336L2Y3   A0A224YX91   A0A195CKR6   A0A0A1X8M6   A0A0C9S360   A0A131YXL0   L7M9Q6   A0A1Y1MI36   A0A0L7R1I7   W8BKX8   B3P1K0   B4QUP5   A0A034WVN9   B4HIQ0   Q9VGW6   B4PLD4   B3M291   A0A1E1X2S1   B4NJY3   B4KC98   A0A0L0BQ18   A0A1I8NCC4   A0A0P4W8T1   U5ELZ4   A0A0M5J5H1   A0A1W4W052   A0A1Z5L9M3   R7V2U4   A0A210Q0K1   A0A3B0JQH7   Q561P5   A0A1I8Q5I2   B4JHM4   A0A2R5LIX8   A0A293LWJ3   A0A1L8GNF3   P55862   K7J409   A0A099ZFS6   A0A091HGL6   A0A2I0USR1   Q5ZKL0   A0A232FEX0   A0A131XFY4   V5HPK2   A0A1L8GGS4   B4M5I3   Q6PCI7  

PDB

6HV9     E-value=8.4023e-164,     Score=1483

KEGG

101746319    K02209 DNA replication licensing factor MCM5 [EC:3.6.4.12] | (RefSeq) DNA replication licensing fact

PATHWAY

03030    DNA replication - Bombyx mori (domestic silkworm)

GO

GO:0005634   GO:0006270   GO:0005524   GO:0004386   GO:0042555   GO:0003688   GO:0003677   GO:0006260   GO:0000727   GO:0032508   GO:0006267   GO:0003697   GO:0042023   GO:0043138   GO:0000712   GO:0030261   GO:0005829   GO:0051321   GO:0000278   GO:0043066   GO:0030174   GO:0006268   GO:0043009   GO:0000785   GO:0007049   GO:0005654   GO:0003678   GO:0003682   GO:0015930  

PANTHER

PTHR11630  

Subcellular location

Nucleus  
Cytoplasm  
Cytosol  

Length:

729

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00321

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00003

outside

1  -  729

 

 

Pi

255.7237

Theta

190.060606

Tajima's D

0.721308

CLR

0.212317

CSRT

0.579821008949553

Interpretation

Uncertain