Silkworm Genome Informatics Database

Gene

KWMTBOMO15247

Pre Gene Modal

BGIBMGA005167

Annotation

PREDICTED:_flavin-dependent_monooxygenase_FMO2_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr25(-):7343371-7350343 View in EpiBrowser

Full name

Flavin-containing monooxygenase       + More
Dimethylaniline monooxygenase [N-oxide-forming]
Senecionine N-oxygenase

Location in the cell

Cytoplasmic Reliability : 3.326

CDS

ATGAAACGGGTTTGTGTTATCGGAGCCGGCATAGCTGGTCTCTCATCGGCAAGATATTTAAAGGAAGAAGGAATCGACTTCGTTGTATTCGAAGCCACGAAGTATATTGGTGGTACGTGGCGTTACGATCCAAGAGTTGGTACCGATGAAAATGGTCTACCATTGCACACCAGCATGTACAAGCATTTACACACGAATTTACCAAAACCAACGATGGAATTAAGAGGTTTCCCATTACCAGATGGAATACCATCGTTTCCTAGTTGGAAAATTTATTATGATTACTTGAAAGATTACGCTAAGCATTTTGACATAGAAAAATATATACAGTTCCGACACAATGTTACTTTGGTGAGACGAGAACAAAACGTTTGGAAAGTGACACACGAACACGTCATCACTGGGGAGGTATTTGAAGAAAATTATGACTACGTCATAGTCGGTAACGGGCATTTTAGTACACCGAACATGCCTAACATACGGGGAGAGAAACTGTTCAAAGGAACAATAATCCACAGTCACGACTACCGTGTACCGGACGTTTACAAAGATCGCAGGGTGCTTGTTGTCGGGGCGGGTCCATCAGGAATGGACATAGGCCTTGACGTGGCTGAATGCAGTAAGAGCCTCCTACACAGCCACCATTCTAAAGTGAACTTCAGAACACCGTTCCCACCGCATTACGTGAGGAAGCCTGACGTAAAGGAATTCAATGAGACTGGAGTTATTTTTGTGGATGGAACTTATGAGGAAATCGATGACGTCATCTACTGCACTGGTTTTCAATACGACTATCCATTCCTAGACAAGACCTGTGGTCTAGATATAGACCCGCACAGCGTGGTCCCCCTGTACAAATACATGGTGAACATCCGTCAGCCGAGCATGGTCATTTTGGGCTTAGTTGTTCGCGCTTGTCTCGTCGTAGCTCTAGACGCCCAGGCACGGTATGCAACGGCCTTAATAAAAGGAAACTTCACTTTGCCGAGCGAAGCAGAAATGATGGACGAATGGCAGAGACGGGCTGATGCGATCCGATCTAAAGGACTAAGAATGTCTCACATACACACCCTTGCAGAAAAAGAAGACGAATATTACGCTGAACTATCGGAGCAATCTGGAATAGAAAGAGTTCCACCGGTCATGTTCAAGATACGCGCCATGGACATTGAAGCTAAGCTTGAAAACCTGTACACGTACAGGCACTACGTGTACAAAGTCATTGACGACAACACGTTCGTTAGGACCCTGGAAAAAGAACAGATCAACGATACTTTGGTGATTTAG

Protein

MKRVCVIGAGIAGLSSARYLKEEGIDFVVFEATKYIGGTWRYDPRVGTDENGLPLHTSMYKHLHTNLPKPTMELRGFPLPDGIPSFPSWKIYYDYLKDYAKHFDIEKYIQFRHNVTLVRREQNVWKVTHEHVITGEVFEENYDYVIVGNGHFSTPNMPNIRGEKLFKGTIIHSHDYRVPDVYKDRRVLVVGAGPSGMDIGLDVAECSKSLLHSHHSKVNFRTPFPPHYVRKPDVKEFNETGVIFVDGTYEEIDDVIYCTGFQYDYPFLDKTCGLDIDPHSVVPLYKYMVNIRQPSMVILGLVVRACLVVALDAQARYATALIKGNFTLPSEAEMMDEWQRRADAIRSKGLRMSHIHTLAEKEDEYYAELSEQSGIERVPPVMFKIRAMDIEAKLENLYTYRHYVYKVIDDNTFVRTLEKEQINDTLVI

Description

NADPH-dependent monooxygenase that detoxifies senecionine and similar plant alkaloids that are ingested by the larvae. Is active towards a narrow range of related substrates with highest activity towards senecionine, followed by seneciphylline, retrorsine, monocrotaline, senecivernine, axillarine and axillaridine.

Catalytic Activity

H(+) + N,N-dimethylaniline + NADPH + O2 = H2O + N,N-dimethylaniline N-oxide + NADP(+)
NADPH + O2 + senecionine = H2O + NADP(+) + senecionine N-oxide

Cofactor

FAD

Biophysicochemical Properties

1.4 uM for senecionine
1.3 uM for NADPH
12.5 uM for monocrotaline

Subunit

Homotetramer.

Miscellaneous

Larvae store pyrrolizidine alkaloids from their host plant S.jacobaeae as non-toxic N-oxides. This serves as protection against insectivores; after ingestion of the larvae, the N-oxides are reduced in the gut of the insectivores to form toxic alkaloids (PubMed:11972041).

Similarity

Belongs to the FMO family.

Keywords

Direct protein sequencing FAD Flavoprotein Monooxygenase NADP Oxidoreductase Secreted Signal

Feature

chain Flavin-containing monooxygenase

Uniprot

D7PGZ5 A0A194RQ38 A0A286R1L3 A0A2H1VFD8 A0A194PI12 A0A2W1BHH5 + More
D7PGZ9 A0A2A4JPB9 A0A2A4JPF0 A0A0N1IQD4 D7PH00 A0A0N1I9T0 A0A3S2LIM8 D7PGZ8 A0A2W1BRW5 A0A2A4JMJ1 A0A194PGD2 H9J674 D7PGZ3 A0A194RKS1 A0A286R1L9 A0A212F1T7 D7PGZ4 D6CHF2 Q8MP06 A0A212ERB5 D6CHF0 A0A2A3EBU3 A0A087ZTT5 D7PGZ7 A0A232EWQ4 A0A0L7RBA9 A0A154PME0 A0A0N1IDD1 K7IN99 B0X209 D7PGZ6 A0A2A4ITS9 A0A336MKL4 A0A1B0CSN7 A0A1L8E571 A0A182SUY5 A0A182MKS9 A0A286R1N3 A0A182R8W5 A0A182J3T6 D7PH01 A0A1B0D7G2 A0A1S4EWV8 A0A3B0JIE5 Q9W1E9 Q962N6 A0A194R599 A0A182GF86 A0A0J9U9S6 S4P1Z8 B4I8X8 Q17N35 A0A146M349 B4MRM2 A0A0A9Z1L3 A0A0A9Z036 J9JV88 A0A1A9XZL9 B3NQ36 B4GD01 A0A212FDS2 A0A182XFN9 A0A182W8C5 A0A2H1V3V2 A0A1I8N0T0 A0A182JS82 W5JX01 B4KMB0 A0A0K8TLH5 B3MCN8 B4MRM1 B4PAB2 A0A1I8N0S7 A0A1J1IBQ1 A0A084W6V0 A0A182IWA4 A0A1B0B9Z9 A0A182QBZ2 A0A182FBL6 A0A0L0CIS3 U5ETD5 A0A0N0U3V8 R4G4T9 A0A1I8P926 T1HWV6 A0A1I8PUP4 A0A224XCP3 D6CHF1 A0A2M3Z5D9 A0A182QJN8 A0A2H8TMC0

EC Number

1.-.-.-
1.14.13.8
1.14.13.101

Pubmed

20454663 26354079 28756777 19121390 22118469 11972041 + More
9182998 28648823 20075255 10731132 12537568 12537572 12537573 12537574 16110336 17569856 17569867 26109357 26109356 15352153 26483478 22936249 23622113 17994087 17510324 26823975 25401762 25315136 20920257 23761445 26369729 18057021 17550304 24438588 26108605

EMBL

GU564656 ADH16748.1 KQ460045 KPJ18136.1 MF465794 ASV48992.1 + More
ODYU01002253 SOQ39491.1 KQ459604 KPI92364.1 KZ150046 PZC74502.1 GU564660 ADH16752.1 NWSH01000977 PCG73293.1 PCG73292.1 LADI01010417 KPJ20713.1 GU564661 ADH16753.1 KQ459383 KPJ01246.1 RSAL01000102 RVE47468.1 GU564659 ADH16751.1 PZC74503.1 PCG73295.1 KPI92362.1 BABH01019965 BABH01019966 BABH01019967 GU564654 ADH16746.1 KPJ18137.1 MF465793 ASV48991.1 AGBW02010802 OWR47700.1 GU564655 ADH16747.1 FN649424 CBI83748.1 AJ420233 AGBW02013066 OWR44027.1 FN649422 CBI83746.1 KZ288291 PBC29207.1 GU564658 ADH16750.1 NNAY01001829 OXU22776.1 KQ414617 KOC68207.1 KQ434977 KZC12907.1 KQ459169 KPJ03412.1 DS232276 EDS38939.1 BABH01041995 GU564657 ADH16749.1 NWSH01008153 PCG62674.1 UFQS01001190 UFQT01001190 SSX09607.1 SSX29403.1 AJWK01026328 GFDF01000402 JAV13682.1 AXCM01001621 MF465795 ASV48993.1 GU564662 ADH16754.1 AJVK01003957 AJVK01003958 AJVK01003959 OUUW01000001 SPP73189.1 AE013599 AY051603 AAF47118.1 AAK93027.1 AF405243 AAK94940.1 KQ460779 KPJ12415.1 JXUM01059552 KQ562059 KXJ76794.1 CM002911 KMY96190.1 GAIX01012245 JAA80315.1 CH480824 EDW57053.1 CH477202 EAT48108.1 GDHC01004411 JAQ14218.1 CH963850 EDW74761.1 GBHO01006366 GBHO01006365 JAG37238.1 JAG37239.1 GBHO01006368 GBHO01006367 GBRD01005428 GBRD01005427 GBRD01005425 GBRD01005423 JAG37236.1 JAG37237.1 JAG60393.1 ABLF02031373 CH954179 EDV56909.1 CH479181 EDW31539.1 AGBW02009018 OWR51848.1 ODYU01000543 SOQ35479.1 ADMH02000071 ETN67910.1 CH933808 EDW09798.1 GDAI01002396 JAI15207.1 CH902619 EDV36272.1 KPU76107.1 EDW74760.1 CM000158 EDW92438.1 CVRI01000047 CRK97656.1 ATLV01020959 KE525310 KFB45944.1 JXJN01010647 AXCN02002299 JRES01000340 KNC32117.1 GANO01002830 JAB57041.1 KQ435851 KOX70828.1 GAHY01000476 JAA77034.1 ACPB03023670 GFTR01006259 JAW10167.1 FN649423 CBI83747.1 GGFM01002990 MBW23741.1 GFXV01003498 MBW15303.1

Proteomes

UP000053240 UP000053268 UP000218220 UP000283053 UP000005204 UP000007151 + More
UP000242457 UP000005203 UP000215335 UP000053825 UP000076502 UP000002358 UP000002320 UP000092461 UP000075901 UP000075883 UP000075900 UP000075880 UP000092462 UP000268350 UP000000803 UP000069940 UP000249989 UP000001292 UP000008820 UP000007798 UP000007819 UP000092443 UP000008711 UP000008744 UP000076407 UP000075920 UP000095301 UP000075881 UP000000673 UP000009192 UP000007801 UP000002282 UP000183832 UP000030765 UP000092460 UP000075886 UP000069272 UP000037069 UP000053105 UP000095300 UP000015103

PRIDE

D7PGZ5 H9J674 D7PGZ3 D7PGZ4 Q8MP06 D7PGZ7 + More
D7PGZ6 Q9W1E9 Q962N6

Pfam

PF00743 FMO-like

Interpro

IPR000960   Flavin_mOase        + More
IPR036188   FAD/NAD-bd_sf
IPR020946   Flavin_mOase-like

SUPFAM

SSF51905 SSF51905

Gene 3D

3.50.50.60

ProteinModelPortal

D7PGZ5 A0A194RQ38 A0A286R1L3 A0A2H1VFD8 A0A194PI12 A0A2W1BHH5 + More
D7PGZ9 A0A2A4JPB9 A0A2A4JPF0 A0A0N1IQD4 D7PH00 A0A0N1I9T0 A0A3S2LIM8 D7PGZ8 A0A2W1BRW5 A0A2A4JMJ1 A0A194PGD2 H9J674 D7PGZ3 A0A194RKS1 A0A286R1L9 A0A212F1T7 D7PGZ4 D6CHF2 Q8MP06 A0A212ERB5 D6CHF0 A0A2A3EBU3 A0A087ZTT5 D7PGZ7 A0A232EWQ4 A0A0L7RBA9 A0A154PME0 A0A0N1IDD1 K7IN99 B0X209 D7PGZ6 A0A2A4ITS9 A0A336MKL4 A0A1B0CSN7 A0A1L8E571 A0A182SUY5 A0A182MKS9 A0A286R1N3 A0A182R8W5 A0A182J3T6 D7PH01 A0A1B0D7G2 A0A1S4EWV8 A0A3B0JIE5 Q9W1E9 Q962N6 A0A194R599 A0A182GF86 A0A0J9U9S6 S4P1Z8 B4I8X8 Q17N35 A0A146M349 B4MRM2 A0A0A9Z1L3 A0A0A9Z036 J9JV88 A0A1A9XZL9 B3NQ36 B4GD01 A0A212FDS2 A0A182XFN9 A0A182W8C5 A0A2H1V3V2 A0A1I8N0T0 A0A182JS82 W5JX01 B4KMB0 A0A0K8TLH5 B3MCN8 B4MRM1 B4PAB2 A0A1I8N0S7 A0A1J1IBQ1 A0A084W6V0 A0A182IWA4 A0A1B0B9Z9 A0A182QBZ2 A0A182FBL6 A0A0L0CIS3 U5ETD5 A0A0N0U3V8 R4G4T9 A0A1I8P926 T1HWV6 A0A1I8PUP4 A0A224XCP3 D6CHF1 A0A2M3Z5D9 A0A182QJN8 A0A2H8TMC0

PDB

5NMX E-value=1.69976e-73, Score=702

KEGG

100462671 no KO assigned | (RefSeq) FMO2; flavin-dependent monooxygenase FMO2

GO

GO:0004499 GO:0050661 GO:0050660 GO:0005789 GO:0004497 GO:0016021 GO:0009820 GO:0055114 GO:0033784 GO:0005576 GO:0051289 GO:0005829 GO:0005739 GO:0043231 GO:0016491 GO:0005524 GO:0016020 GO:0003677 GO:0008408 GO:0005112 GO:0015930

Subcellular location

Microsome membrane
Endoplasmic reticulum membrane
Secreted

SignalP

Position: 1 - 17, Likelihood: 0.621873

Length:

428

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

1.25885

Exp number, first 60 AAs:

0.02288

Total prob of N-in:

0.03583

outside

1 - 428

Pi

239.426599

Theta

174.857029

Tajima's D

1.048834

CLR

0.394419

CSRT

0.670166491675416

Interpretation

Uncertain

Sequence

Summary

Ontologies

Topology

Population Genetic Test Statistics