Gene

KWMTBOMO15246  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA005015

Annotation

flavin-dependent_monooxygenase_FMO1_[Bombyx_mori]

Location

Bomo_Chr25(+):7321534-7339554      View in EpiBrowser

Full name

Flavin-containing monooxygenase       + More
Senecionine N-oxygenase      
Dimethylaniline monooxygenase [N-oxide-forming]      

Location in the cell

Cytoplasmic   Reliability : 1.78

CDS

ATGGAATATTACGACTTTCCATTTCCCGAGGGAACCCCGTCGTATCCATCGGCAACGTGCTTCCTCGATTACTTAAAATCGTTTGTGAAGCATTTCGATTTACTCAGTCATATTCAATTGCGCAGCTTGGTCACATCAGTGAAGTGGGCGGGCAATCACTGGAACCTTACTTACACAAAGACGGACACCAAGGAAAATGTAACTGAGACGTGTGACTTCATTGTGGTAGCAAATGGCCCTTATAATACTCCTGTGTGGCCCAAGTACGACGGAATAGACACATTCGAAGGCAGCATGATCCACAGCCATGATTACAAAGATCGGAAGGCGTACAAAAACAGAAAGGTGCTTATAGTAGGAGCTGGAGCTTCAGGCTTGGATCTCGCGATACAGTTGTCCAACGTGACCGCCAAATTAGTTCACAGCCACCACTTGGTGTATAACGAACCGAAATTTTTCGATGGTTACGTGAAGAAACCAGACATAATGGCGTTTACTCCTAAAGGCGTTATTTTCCGGGACGAATCATTTGAGGAATTGGATGATGTAATTTTTTGCACAGGTTATGACTTCAACCACCCGTTTCTAGACGAGAGCTGCGGTGTTACCTCAACCGCCAAATTTGTACTGCCTCTCCATAAGCAACTTGTCAATATAAAACATCCCAGCATGGTATTTCTTGGAATCGCCAAGAAGATTATAACAAGAGTTATGGATGCTCAGGCTGAATACGCAGCACTACTAGCTTCGGGTAAATTGAAGCTTCCATCACAGGAGGAAATGTTGAATTCTTGGCTGAAACACATCTCTTCCCTACAGGTTAAGGGCATGAAAATTATCGATTTAAACGTAGTCGGTTCGGAAATGGATCAATATTTCGGCAATTTAACTGAAGAAGCTGGTGTGGTGAGAGCGCCCCCGGTACTCACAGCGATCAGGGATTTCAACGGCGTCAACCGTTTGGATGATTTGCTCAACTACCGCGAATACGACTATAGCATTATTGATAATTTTCATTACGAAAGAAAATATAATCCAAGGCAGAACATACCTTGCCCTGTCGAAGTGTGA

Protein

MEYYDFPFPEGTPSYPSATCFLDYLKSFVKHFDLLSHIQLRSLVTSVKWAGNHWNLTYTKTDTKENVTETCDFIVVANGPYNTPVWPKYDGIDTFEGSMIHSHDYKDRKAYKNRKVLIVGAGASGLDLAIQLSNVTAKLVHSHHLVYNEPKFFDGYVKKPDIMAFTPKGVIFRDESFEELDDVIFCTGYDFNHPFLDESCGVTSTAKFVLPLHKQLVNIKHPSMVFLGIAKKIITRVMDAQAEYAALLASGKLKLPSQEEMLNSWLKHISSLQVKGMKIIDLNVVGSEMDQYFGNLTEEAGVVRAPPVLTAIRDFNGVNRLDDLLNYREYDYSIIDNFHYERKYNPRQNIPCPVEV

Description

NADPH-dependent monooxygenase that detoxifies senecionine and similar plant alkaloids that are ingested by the larvae. Is active towards a narrow range of related substrates with highest activity towards senecionine, followed by seneciphylline, retrorsine, monocrotaline, senecivernine, axillarine and axillaridine.

Catalytic Activity

NADPH + O2 + senecionine = H2O + NADP(+) + senecionine N-oxide
H(+) + N,N-dimethylaniline + NADPH + O2 = H2O + N,N-dimethylaniline N-oxide + NADP(+)

Cofactor

FAD

Biophysicochemical Properties

1.4 uM for senecionine
1.3 uM for NADPH
12.5 uM for monocrotaline

Subunit

Homotetramer.

Miscellaneous

Larvae store pyrrolizidine alkaloids from their host plant S.jacobaeae as non-toxic N-oxides. This serves as protection against insectivores; after ingestion of the larvae, the N-oxides are reduced in the gut of the insectivores to form toxic alkaloids (PubMed:11972041).

Similarity

Belongs to the FMO family.

Keywords

Direct protein sequencing   FAD   Flavoprotein   Monooxygenase   NADP   Oxidoreductase   Secreted   Signal  

Feature

chain  Flavin-containing monooxygenase

Uniprot

D7PGZ3   D7PGZ4   H9J674   A0A3S2LIM8   D7PGZ8   A0A2W1BRW5   + More
A0A2A4JMJ1   A0A286R1L9   A0A194PGD2   A0A194RKS1   A0A212F1T7   A0A2H1VF51   D6CHF2   D6CHF1   D6CHF0   Q8MP06   A0A212FDL5   D6CHF3   A0A212FDS2   D6CHF7   A0A2H1VFD8   D7PGZ5   A0A286R1L3   D6CHF5   A0A194RQ38   D6CHF4   A0A0N1IQD4   D6CHF6   D6CHF8   A0A2W1BHH5   A0A0N1I9T0   D7PGZ9   A0A2A4JPB9   A0A2A4JPF0   D7PH00   A0A194PI12   H9J6M5   T1HWV6   R4G4T9   D7PGZ7   A0A212ERB5   S4P1Z8   A0A286R1N3   D7GXL5   D7PGZ6   A0A1B0CSN7   A0A2H8TMC0   A0A0N1IDD1   A0A1L8E4E9   D7PH01   A0A087ZTT5   A0A224XCP3   A0A084W6V0   J9JV88   A0A2A3EBU3   A0A154PME0   A0A1B0D7G2   A0A1L8E571   U5ETD5   A0A026WCD8   A0A182R8W8   A0A182QJN8   A0A2H1V3V2   A0A336MKL4   E2A9M6   A0A182PUE9   A0A194R599   A0A182YE75   A0A182IWA4   A0A0N0U3V8   A0A2M3Z5D9   A0A182SW70   Q17N35   A0A182FBL5   A0A1I8M338   T1PI45   W5JX01   A0A0K8WHR4   A0A182LSD8   A0A1S4EWV8   A0A146M349   A0A3B0JIE5   B4I8X8   W5JX83   A0A0A9Z036   A0A182NVD3   A0A2M4BPB0   A0A0A9Z1L3   A0A195BQ08   A0A0L0CIS3   A0A0C9R0F3   A0A034W0H1   A0A0J9U9S6   A0A2M4BPF2   N6TLD0   A0A2A4ITS9   U4UI72   A0A2M4BNW4   A0A2W1BRB6  

EC Number

1.-.-.-
1.14.13.101
1.14.13.8

Pubmed

20454663    19121390    28756777    26354079    22118469    11972041    + More
9182998    23622113    18362917    19820115    24438588    24508170    30249741    20798317    25244985    17510324    25315136    20920257    23761445    26823975    17994087    25401762    26108605    25348373    22936249    23537049   

EMBL

GU564654    ADH16746.1    GU564655    ADH16747.1    BABH01019965    BABH01019966    + More
BABH01019967    RSAL01000102    RVE47468.1    GU564659    ADH16751.1    KZ150046    PZC74503.1    NWSH01000977    PCG73295.1    MF465793    ASV48991.1    KQ459604    KPI92362.1    KQ460045    KPJ18137.1    AGBW02010802    OWR47700.1    ODYU01002253    SOQ39490.1    FN649424    CBI83748.1    FN649423    CBI83747.1    FN649422    CBI83746.1    AJ420233    AGBW02009018    OWR51849.1    FN649425    CBI83749.1    OWR51848.1    FN649429    CBI83753.1    SOQ39491.1    GU564656    ADH16748.1    MF465794    ASV48992.1    FN649427    CBI83751.1    KPJ18136.1    FN649426    CBI83750.1    LADI01010417    KPJ20713.1    FN649428    CBI83752.1    FN649430    CBI83754.1    PZC74502.1    KQ459383    KPJ01246.1    GU564660    ADH16752.1    PCG73293.1    PCG73292.1    GU564661    ADH16753.1    KPI92364.1    ACPB03023670    GAHY01000476    JAA77034.1    GU564658    ADH16750.1    AGBW02013066    OWR44027.1    GAIX01012245    JAA80315.1    MF465795    ASV48993.1    GG694257    EFA13493.1    BABH01041995    GU564657    ADH16749.1    AJWK01026328    GFXV01003498    MBW15303.1    KQ459169    KPJ03412.1    GFDF01000456    JAV13628.1    GU564662    ADH16754.1    GFTR01006259    JAW10167.1    ATLV01020959    KE525310    KFB45944.1    ABLF02031373    KZ288291    PBC29207.1    KQ434977    KZC12907.1    AJVK01003957    AJVK01003958    AJVK01003959    GFDF01000402    JAV13682.1    GANO01002830    JAB57041.1    KK107274    QOIP01000008    EZA53735.1    RLU19303.1    AXCN02002299    ODYU01000543    SOQ35479.1    UFQS01001190    UFQT01001190    SSX09607.1    SSX29403.1    GL437918    EFN69828.1    KQ460779    KPJ12415.1    KQ435851    KOX70828.1    GGFM01002990    MBW23741.1    CH477202    EAT48108.1    KA647573    AFP62202.1    ADMH02000071    ETN67910.1    GDHF01001623    JAI50691.1    AXCM01001621    GDHC01004411    JAQ14218.1    OUUW01000001    SPP73189.1    CH480824    EDW57053.1    ETN67909.1    GBHO01006368    GBHO01006367    GBRD01005428    GBRD01005427    GBRD01005425    GBRD01005423    JAG37236.1    JAG37237.1    JAG60393.1    GGFJ01005701    MBW54842.1    GBHO01006366    GBHO01006365    JAG37238.1    JAG37239.1    KQ976424    KYM88590.1    JRES01000340    KNC32117.1    GBYB01001475    JAG71242.1    GAKP01009862    GAKP01009861    JAC49091.1    CM002911    KMY96190.1    GGFJ01005743    MBW54884.1    APGK01019955    KB740150    ENN81259.1    NWSH01008153    PCG62674.1    KB632173    ERL89585.1    GGFJ01005629    MBW54770.1    KZ150001    PZC75310.1   

Proteomes

UP000005204    UP000283053    UP000218220    UP000053268    UP000053240    UP000007151    + More
UP000015103    UP000007266    UP000092461    UP000005203    UP000030765    UP000007819    UP000242457    UP000076502    UP000092462    UP000053097    UP000279307    UP000075900    UP000075886    UP000000311    UP000075885    UP000076408    UP000075880    UP000053105    UP000075901    UP000008820    UP000069272    UP000095301    UP000000673    UP000075883    UP000268350    UP000001292    UP000075884    UP000078540    UP000037069    UP000019118    UP000030742   

PRIDE

D7PGZ3    D7PGZ4    H9J674    Q8MP06    D7PGZ5    H9J6M5    + More
D7PGZ7    D7PGZ6   

Pfam

PF00743   FMO-like

Interpro

IPR020946   Flavin_mOase-like        + More
IPR000960   Flavin_mOase       
IPR036188   FAD/NAD-bd_sf       

SUPFAM

SSF51905   SSF51905       

Gene 3D

3.50.50.60    

ProteinModelPortal

D7PGZ3   D7PGZ4   H9J674   A0A3S2LIM8   D7PGZ8   A0A2W1BRW5   + More
A0A2A4JMJ1   A0A286R1L9   A0A194PGD2   A0A194RKS1   A0A212F1T7   A0A2H1VF51   D6CHF2   D6CHF1   D6CHF0   Q8MP06   A0A212FDL5   D6CHF3   A0A212FDS2   D6CHF7   A0A2H1VFD8   D7PGZ5   A0A286R1L3   D6CHF5   A0A194RQ38   D6CHF4   A0A0N1IQD4   D6CHF6   D6CHF8   A0A2W1BHH5   A0A0N1I9T0   D7PGZ9   A0A2A4JPB9   A0A2A4JPF0   D7PH00   A0A194PI12   H9J6M5   T1HWV6   R4G4T9   D7PGZ7   A0A212ERB5   S4P1Z8   A0A286R1N3   D7GXL5   D7PGZ6   A0A1B0CSN7   A0A2H8TMC0   A0A0N1IDD1   A0A1L8E4E9   D7PH01   A0A087ZTT5   A0A224XCP3   A0A084W6V0   J9JV88   A0A2A3EBU3   A0A154PME0   A0A1B0D7G2   A0A1L8E571   U5ETD5   A0A026WCD8   A0A182R8W8   A0A182QJN8   A0A2H1V3V2   A0A336MKL4   E2A9M6   A0A182PUE9   A0A194R599   A0A182YE75   A0A182IWA4   A0A0N0U3V8   A0A2M3Z5D9   A0A182SW70   Q17N35   A0A182FBL5   A0A1I8M338   T1PI45   W5JX01   A0A0K8WHR4   A0A182LSD8   A0A1S4EWV8   A0A146M349   A0A3B0JIE5   B4I8X8   W5JX83   A0A0A9Z036   A0A182NVD3   A0A2M4BPB0   A0A0A9Z1L3   A0A195BQ08   A0A0L0CIS3   A0A0C9R0F3   A0A034W0H1   A0A0J9U9S6   A0A2M4BPF2   N6TLD0   A0A2A4ITS9   U4UI72   A0A2M4BNW4   A0A2W1BRB6  

PDB

5NMX     E-value=4.73285e-46,     Score=465

KEGG

100462670    no KO assigned | (RefSeq) FMO1; flavin-dependent monooxygenase FMO1

GO

GO:0050661   GO:0050660   GO:0016021   GO:0004499   GO:0004497   GO:0033784   GO:0009820   GO:0055114   GO:0005576   GO:0051289   GO:0005789   GO:0005739   GO:0005829   GO:0016491   GO:0005524   GO:0016020   GO:0003677   GO:0008408   GO:0005112   GO:0015930  

Subcellular location

Secreted  
Microsome membrane  
Endoplasmic reticulum membrane  

Length:

356

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.17517

Exp number, first 60 AAs:

0.00411

Total prob of N-in:

0.01043

outside

1  -  356

 

 

Pi

259.755149

Theta

162.254028

Tajima's D

1.842205

CLR

0.085805

CSRT

0.856407179641018

Interpretation

Uncertain