Gene

KWMTBOMO15225

Annotation

hemolymph_proteinase_5_[Danaus_plexippus]

Location

Bomo_Chr25(-):7142611-7150313      View in EpiBrowser

Full name

Melanization protease 1      

Location in the cell

Extracellular   Reliability : 2.585

CDS

ATGCTGCAGACGTTTTTTTTAGTCGTCACATCGATCCTGCAAATACAAAGTTTAGAATTTGGAGAGAGTTGTTTTGCAAATGATGAACCGGGAACGTGTATCGCCCTCTCAGAATGCAAGCCAGTTTTATCATACATCGAAAAGTTTGGAAATCAAATCCCTAATAATTTGCTACAGAAGTTTCAAGATCTGACGTGCGGCTATGACCACAGCGATCCCCTGGTTTGCTGCACTACATCCCAACACATAGCTGGATACTTTAACAATTTTGGTAATCAAAATGGCGGGACTAGCGTACCGAAACGACCGAAGCCCGTTCCTACTAGTAACAGCGGTAATTGGGGCAGTCAACCAAGCAAGGAACAAACAACAAGCAACCGACCGACTACTGCTGATACTGATATTAATAAGAGCGGCCGTGATGACGACTTTCCGGACATTCGTCAGAACCCTAACTTGAGTTTACTATCAAGTAATTGTGGAACGATAGAAAGCGATAGAATATTCGGAGGGAATCGTACCCGACTGTTCGAGATGCCGTGGATGGTGCTTCTTTCCTACGATTCAGATCGTGGAACAAAACTGAATTGCGGAGGAACACTTATAAACGAATGGTATGTTTTAACGGCCGCGCATTGTGTATCGTTCTTAGGAAATAGATTAACATTAAAACACGTTATCCTGGGAGAATATGACACAAGGCAAGATCCTGACTGCGAAAGATCGGAAGGAAAGGAATATTGCGCGGCTGGTATAATCACGGCAGAATTCGACGAAGTAATTCCTCATACAGGCTACACACCACAAACATTAATCGATGATATAGCATTAATCAGACTGAAAGAGCCTGCCGACTTTGATTTAGATAATATTAAAGCGATTTGCTTACCGATTACTCCTGAACTGCAAAGGGAGCCTCTTGTCGATAACTTTGGCGTGGTAGCAGGTTGGGGAGTGACAGAAGAAGGACTACAATCTCCTGTTTTGCTTAGTGTACAGCTGCCAATTCTCAGCAAAGAAAAATGTTTGCAAGACTATTCCCAATATTCCTTGAAAATCAACGACAAGCAACTCTGCGCCGGCGGTTTGCACGATAAGGATTCATGCGCCGGGGATTCTGGTGGCCCTTTGCTGTATCCAGGGAAAGTTGGCTCAACTGGCGTCCGATATGTTCAGAGAGGCATAGTCTCGTTTGGGTCGAAGCGGTGTGGTATCAGCCTGCCGGGAGTGTACACGAATGTTGCATACTACATGGATTGGATCTTGAATAATATTAGAAGTTGA

Protein

MLQTFFLVVTSILQIQSLEFGESCFANDEPGTCIALSECKPVLSYIEKFGNQIPNNLLQKFQDLTCGYDHSDPLVCCTTSQHIAGYFNNFGNQNGGTSVPKRPKPVPTSNSGNWGSQPSKEQTTSNRPTTADTDINKSGRDDDFPDIRQNPNLSLLSSNCGTIESDRIFGGNRTRLFEMPWMVLLSYDSDRGTKLNCGGTLINEWYVLTAAHCVSFLGNRLTLKHVILGEYDTRQDPDCERSEGKEYCAAGIITAEFDEVIPHTGYTPQTLIDDIALIRLKEPADFDLDNIKAICLPITPELQREPLVDNFGVVAGWGVTEEGLQSPVLLSVQLPILSKEKCLQDYSQYSLKINDKQLCAGGLHDKDSCAGDSGGPLLYPGKVGSTGVRYVQRGIVSFGSKRCGISLPGVYTNVAYYMDWILNNIRS

Description

Serine protease which plays an essential role in the melanization immune response by acting downstream of sp7 to activate prophenoloxidase (PPO1) (PubMed:16861233). May function in diverse Hayan-dependent PPO1-activating cascades that are negatively controlled by different serpin proteins; Spn27A in the hemolymph and Spn77BA in the trachea (PubMed:18854145, PubMed:16861233). Regulation of melanization and PPO1 activation appears to be largely independent of the Toll signaling pathway (PubMed:16861233).

Similarity

Belongs to the peptidase S1 family.
Belongs to the peptidase S1 family. CLIP subfamily.

Keywords

Alternative splicing   Calcium   Complete proteome   Disulfide bond   Glycoprotein   Hydrolase   Metal-binding   Protease   Reference proteome   Serine protease   Signal   Zymogen  

Feature

propeptide  Activation peptide
chain  Melanization protease 1
splice variant  In isoform A.

Uniprot

A0A212F937   G9F9I4   A0A2H1W4A8   A0A2A4IZ28   A0A2W1BQ49   A0A194PG32   + More
I4DNT5   A0A194RL52   Q5MPC9   A0A2J7Q286   A0A2P8YP04   A0A1L8DR27   A0A1L8DQP3   A0A1V1G586   A0A0M9A8Q5   A0A067QFH0   D6WP83   A0A0L7QXJ6   A0A026WIG3   A0A195FEJ5   F4WPR7   A0A195EG59   A0A0C9PIF6   E2C8L1   U5EMJ9   A0A0L7LRR0   D6WP86   A0A3L8D7J0   K7J981   E2C8L2   A0A232EZG2   A0A1B0CMU1   A0A1W6EWF7   A0A2J7Q299   A0A182QQA1   A0A154P3E5   A0A310SR39   A0A182S526   A0A1Y1MBK3   F4WPR8   A0A1V1FKN6   A0A139WF86   A0A0J7NWE6   A0A3F2YU18   E0VXK9   A0A182YKD4   A0A034VLF9   K7J980   Q7QKL1   A0A182MDC4   E2AV85   A0A195EG37   A0A2C9GV88   B4PNV6   A0A1I8JTD0   I4DKD5   A0A154P5I7   A0A182V2M2   A0A0M9A7E2   A0A0L7QXF0   A0A182XUW3   A0A1Y1L107   A0A126GUP6-2   A0A0C9QZC0   A0A034VVW3   U4U606   A0A067QHJ6   A0A0A1WS20   J3JYT9   B4QUZ8   A0A1W4UJW8   A0A1D2MDI9   A0A0C9QBV2  

EC Number

3.4.21.-

Pubmed

22118469    28756777    26354079    22651552    15944088    29403074    + More
28410430    24845553    18362917    19820115    24508170    21719571    20798317    26227816    30249741    20075255    28648823    28004739    20566863    25244985    25348373    12364791    14747013    17210077    17994087    17550304    10731132    12537572    10731138    16861233    18854145    22227521    23537049    25830018    22516182    27289101   

EMBL

AGBW02009640    OWR50265.1    JN033729    AEW46882.1    ODYU01006253    SOQ47925.1    + More
NWSH01004460    PCG65075.1    KZ150014    PZC75016.1    KQ459604    KPI92346.1    AK403129    BAM19575.1    KQ460045    KPJ18149.1    AY672781    AAV91003.1    NEVH01019373    PNF22687.1    PYGN01000457    PSN45975.1    GFDF01005299    JAV08785.1    GFDF01005298    JAV08786.1    FX985343    BAX07356.1    KQ435719    KOX78756.1    KK853628    KDR03823.1    KQ971353    EFA07454.1    KQ414704    KOC63286.1    KK107185    EZA55837.1    KQ981636    KYN38801.1    GL888255    EGI63808.1    KQ978957    KYN27228.1    GBYB01000710    GBYB01000713    JAG70477.1    JAG70480.1    GL453666    EFN75741.1    GANO01000994    JAB58877.1    JTDY01000242    KOB78094.1    EFA07452.2    QOIP01000012    RLU16063.1    AAZX01002213    EFN75742.1    NNAY01001477    OXU23832.1    AJWK01019165    AJWK01019166    AJWK01019167    AJWK01019168    KY563640    ARK20049.1    PNF22708.1    AXCN02000165    KQ434809    KZC06391.1    KQ761039    OAD58341.1    GEZM01037774    JAV81940.1    EGI63809.1    FX985369    BAX07382.1    KYB26592.1    LBMM01001207    KMQ96720.1    DS235833    EEB18115.1    GAKP01015995    JAC42957.1    AAAB01008794    EAA03537.4    AXCM01000867    GL443059    EFN62639.1    KYN27230.1    CM000160    EDW98166.2    APCN01002147    AK401753    BAM18375.1    KZC06390.1    KOX78755.1    KOC63285.1    GEZM01073843    JAV64787.1    AE014297    AF145611    BT125977    AAD38586.1    ADX35956.1    GBYB01001065    JAG70832.1    GAKP01013295    GAKP01013294    JAC45658.1    KB631684    ERL85365.1    KDR03824.1    GBXI01012856    JAD01436.1    BT128420    AEE63377.1    CM000364    EDX11579.1    LJIJ01001731    ODM90951.1    GBYB01000714    GBYB01006695    JAG70481.1    JAG76462.1   

Proteomes

UP000007151    UP000218220    UP000053268    UP000053240    UP000235965    UP000245037    + More
UP000053105    UP000027135    UP000007266    UP000053825    UP000053097    UP000078541    UP000007755    UP000078492    UP000008237    UP000037510    UP000279307    UP000002358    UP000215335    UP000092461    UP000075886    UP000076502    UP000075900    UP000036403    UP000075881    UP000009046    UP000076408    UP000007062    UP000075883    UP000000311    UP000075884    UP000002282    UP000075840    UP000075903    UP000076407    UP000000803    UP000030742    UP000000304    UP000192221    UP000094527   

PRIDE

A0A126GUP6   

Pfam

PF00089   Trypsin        + More
PF12032   CLIP
PF13019   Sde2_N_Ubi

Interpro

IPR018114   TRYPSIN_HIS        + More
IPR001254   Trypsin_dom       
IPR009003   Peptidase_S1_PA       
IPR001314   Peptidase_S1A       
IPR033116   TRYPSIN_SER       
IPR022700   CLIP       
IPR038565   CLIP_sf       
IPR024974   Sde2_N       
IPR029071   Ubiquitin-like_domsf       

SUPFAM

SSF50494   SSF50494        + More
SSF54236   SSF54236       

Gene 3D

2.20.20.90    

CDD

cd00190  Tryp_SPc

ProteinModelPortal

A0A212F937   G9F9I4   A0A2H1W4A8   A0A2A4IZ28   A0A2W1BQ49   A0A194PG32   + More
I4DNT5   A0A194RL52   Q5MPC9   A0A2J7Q286   A0A2P8YP04   A0A1L8DR27   A0A1L8DQP3   A0A1V1G586   A0A0M9A8Q5   A0A067QFH0   D6WP83   A0A0L7QXJ6   A0A026WIG3   A0A195FEJ5   F4WPR7   A0A195EG59   A0A0C9PIF6   E2C8L1   U5EMJ9   A0A0L7LRR0   D6WP86   A0A3L8D7J0   K7J981   E2C8L2   A0A232EZG2   A0A1B0CMU1   A0A1W6EWF7   A0A2J7Q299   A0A182QQA1   A0A154P3E5   A0A310SR39   A0A182S526   A0A1Y1MBK3   F4WPR8   A0A1V1FKN6   A0A139WF86   A0A0J7NWE6   A0A3F2YU18   E0VXK9   A0A182YKD4   A0A034VLF9   K7J980   Q7QKL1   A0A182MDC4   E2AV85   A0A195EG37   A0A2C9GV88   B4PNV6   A0A1I8JTD0   I4DKD5   A0A154P5I7   A0A182V2M2   A0A0M9A7E2   A0A0L7QXF0   A0A182XUW3   A0A1Y1L107   A0A126GUP6-2   A0A0C9QZC0   A0A034VVW3   U4U606   A0A067QHJ6   A0A0A1WS20   J3JYT9   B4QUZ8   A0A1W4UJW8   A0A1D2MDI9   A0A0C9QBV2  

PDB

2XXL     E-value=2.19343e-46,     Score=468

KEGG

101738778    no KO assigned | (RefSeq) serine protease easter

GO

GO:0004252   GO:0008236   GO:0016021   GO:0004175   GO:0035006   GO:0046872   GO:0009620   GO:0006508   GO:0046873   GO:0000287   GO:0043874   GO:0016020   GO:0003677   GO:0008408   GO:0005112   GO:0015930  

SignalP

Position:   1 - 17,         Likelihood:  0.986002

 

 

Length:

427

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.13038

Exp number, first 60 AAs:

0.00183

Total prob of N-in:

0.00191

outside

1  -  427

 

 

Pi

189.874502

Theta

178.233668

Tajima's D

1.511313

CLR

0

CSRT

0.789660516974151

Interpretation

Uncertain