Gene

KWMTBOMO15205  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004950

Annotation

PREDICTED:_C-1-tetrahydrofolate_synthase?_cytoplasmic_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr25(+):6713319-6731756      View in EpiBrowser

Full name

C-1-tetrahydrofolate synthase, cytoplasmic      

Location in the cell

Cytoplasmic   Reliability : 1.937 Mitochondrial   Reliability : 1.808

CDS

ATGTGGAAGAATTCTGAAGAATTGGAAGCTGTACAAGAGGTCACTTGCATCTTCGATCCATCTCAGTCCGAGAGAGCAGTCGGGATTGGATCGACCAACGAGACAGTTCTCTGGGCTAAGATGGCACAAATTATATCTGGGATTGAAGTTGCAGGATCTATAGAAAATGACCTGCGTCAACAAGTGACTAGGCTCCGTTCAAAATGGTCTGGCTTCGAGCCCAGGCTTGCCATCGTGCAGGTGGGCGGGCGCGAGGACTCCAACGTCTACATCAGGATGAAGCTGAAGGCAGCTGAGAAAATTGGTATCGCCGCCGAACATATCCGCTTACCAAGGGACATCACTGAAATAGAGCTGTTAGCAAAAATAACGAGTCTGAATGAATCTCCTTCGGTTCACGGCATCATTGTTCAAATGCCCCTTGATTCGGACCACGCGATCGACGCTCATCGAGTCACTGATGCTGTCTCGCCTGATAAAGATGTTGACGGCTTAAACACAATTAACGAAGGTCGAGTAGCAGTAGGTGATCTGTCCGGATTCATTCCTTGCACTCCGGCCGGGTGCGTGGAACTGATTAAGAAGACCGGCGTCACTATAGCTGGCAAAAATGTGGTAGTGCTCGGGCGGAGTCGCATTGTGGGTACTCCGGTGTCAGAGTTGCTGAAATGGGAACACGCTACTGTCACAGTGTGCCATTCAAAGACTAAGAATTTGAGTGAAATTACTAAGACAGCAGAAATACTGGTGGTGGCGATCGGTCGTCCAGAGATGGTCCGAGGTTCTTGGATCAAGCCTGGAGCGGTCGTTATAGACTGTGGTATCAACGCTATTGAGGATCCTACAAAGAAAAGCGGGCAACGTTTAGTGGGCGACGTGGCTTACCAGGAGGCGGTCAAAGTAGCTTCGGCTGTGACTCCTGTGCCGGGCGGCGTGGGGCCCATGACCGTGGCGATGCTCATGCGGAACACCGTGCAGGCAGCGCGCCGACAGCTCGACCGCTTGCTTGCCCCCTCTTGGCCTCTGAAACCGCTTCGCCTCACCCCGATTACGCCACCGCCCAGTGACATCGTAATAGCTCGTTCTCAGAAGCCTAAAGACATCGGAGAGCTGGCGGCAGAGATCGGTCTGTGTCCGAGCGAGGTGTCTCAATATGGACGTACTAAGGCCAAGATTTCACTGTCCGTTCTCGACAGACTACGCAACCAACGCACAGGGAAATACATCGTAGTTGCTGGTATAACTCCGACACCACTTGGTGAAGGTAAAAGCACCACTCTAATAGGTCTGGTGCAAGCGTTGACAGCACATCGCGGGAAGAACGCGTTTGCATGCATGCGGCAACCGAGTCAGGGACCGACCTTTGGCGTTAAAGGGGGTGCTGCTGGAGGGGGCTACTCTCAAGTAATTCCAATGGAGGAGTTCAACCTGCACATGACTGGCGACATTCATGCCGTAACCGCTGCCAACAATCTTCTTGCTGCACAAATGGACGCGCGCATCTTCCACGAGCTGACACAGAAGGACGGACCTCTGTATGACCGTCTCGTCCCCAAGATCAAAGGCGTCAGGAAGTTCTCTCCTATCCAGCTACGCAGACTGAAGAGATTGGGAATCGATAAAACGGACCCTGACTCTTTAACTGCCGAAGAGAGGGTTAAGTTCGCTCGACTCAATATAGACCCAACAAAGATTATGTGGAACAGAGTTGTGGATCTAAACGACAGATATCTTCGAAAGATTACAATAGGCCAGTCTCCTACGGAAAAAGGCTTTACTCGGGAGACTGCTTTCGATATTTCCGTCGCTTCGGAGATCATGGCTATTCTGGCGTTAGGTAATGACGTAGACGACATCAAAGAACGACTCGCCAGCATGGTGGTGGCCCTCGATAAGGACGGCAACGCTGTCACCGCAGACGACTTGGGCATGACGGGAGCGTTGCTGGTGTTGCTACGTGATGCGTTCGAGCCGACGCTGATGCAGAGTCTGGAGGGCACGCCGGTGCTGGTGCACGCAGGACCCTTCGCCAACATCGCGCACGGCTGCTCCTCCATACTGGCCGACAAGATCGCCATGAAGCTGGCCGGTCCCGCTGGATACGTCGCCACTGAAGCTGGATTTGGATCTGATATTGGAATGGAGAAGTTCTTCGACATAAAGTGTCGCGCAAGCGGTGACCGGCCTCACTGCGCGGTCATCGTTGCGACCGTGCGCGCGCTCAAGATGCACGGCGGCGGCCCGCCCGTCTCACCCGGCCTGCCTCTGCATGACGTCTACCTGCAGGAGAACCTAGATCTGCTACACAAAGGTCTATGTAACTTGGGCAAACACATCAGTAACGGCAACAAATTTGGTATTCCGGTCGTTGTAGCTGTCAACAAACACGGAAATGATAGCGCCGCGGAATTGAGTTTGGTGCAAGACTTCGCTCTGCACAACGGCGCGTTCAGGGCGGTGGTGTGTAGCCACTTCACCGACGGCGGGGCGGGCGCTATCGAGCTTGCCGACGCCGTTATCGACGCCTGCAACCGACCCACGCATTTCCAGTACCTATACCCCCTGAGCTTCTCCATCCAGGACAAGATTCACACAATCGCCAGTCAGATGTACGGCGCTTCCGAAATCGAATACACTGAAGAGGTTTTAGAAAAAATTAAAATCTTCACCGAAAAGGGATACGACAAATTTCCGATTTGCATGGCCAAGACTTCGAACTCCTTAACCGGTGATCCCTCCATCAAGGGAGCTCCCACTGACTTCACGCTAAAGATCAACGGAATATTCGTATCGGTAGGCGCCGGCTTCGTGGTGCCCATGGTCGGGGAGATCTCGAAGATGCCCGGACTGCCGACACGACCTAGCATCTACGATATTGACCTCAACACAAAAACAGGGGAAATCGAAGGCCTTTTCTAA

Protein

MWKNSEELEAVQEVTCIFDPSQSERAVGIGSTNETVLWAKMAQIISGIEVAGSIENDLRQQVTRLRSKWSGFEPRLAIVQVGGREDSNVYIRMKLKAAEKIGIAAEHIRLPRDITEIELLAKITSLNESPSVHGIIVQMPLDSDHAIDAHRVTDAVSPDKDVDGLNTINEGRVAVGDLSGFIPCTPAGCVELIKKTGVTIAGKNVVVLGRSRIVGTPVSELLKWEHATVTVCHSKTKNLSEITKTAEILVVAIGRPEMVRGSWIKPGAVVIDCGINAIEDPTKKSGQRLVGDVAYQEAVKVASAVTPVPGGVGPMTVAMLMRNTVQAARRQLDRLLAPSWPLKPLRLTPITPPPSDIVIARSQKPKDIGELAAEIGLCPSEVSQYGRTKAKISLSVLDRLRNQRTGKYIVVAGITPTPLGEGKSTTLIGLVQALTAHRGKNAFACMRQPSQGPTFGVKGGAAGGGYSQVIPMEEFNLHMTGDIHAVTAANNLLAAQMDARIFHELTQKDGPLYDRLVPKIKGVRKFSPIQLRRLKRLGIDKTDPDSLTAEERVKFARLNIDPTKIMWNRVVDLNDRYLRKITIGQSPTEKGFTRETAFDISVASEIMAILALGNDVDDIKERLASMVVALDKDGNAVTADDLGMTGALLVLLRDAFEPTLMQSLEGTPVLVHAGPFANIAHGCSSILADKIAMKLAGPAGYVATEAGFGSDIGMEKFFDIKCRASGDRPHCAVIVATVRALKMHGGGPPVSPGLPLHDVYLQENLDLLHKGLCNLGKHISNGNKFGIPVVVAVNKHGNDSAAELSLVQDFALHNGAFRAVVCSHFTDGGAGAIELADAVIDACNRPTHFQYLYPLSFSIQDKIHTIASQMYGASEIEYTEEVLEKIKIFTEKGYDKFPICMAKTSNSLTGDPSIKGAPTDFTLKINGIFVSVGAGFVVPMVGEISKMPGLPTRPSIYDIDLNTKTGEIEGLF

Catalytic Activity

(6R)-5,10-methylene-5,6,7,8-tetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH
5,10-methenyltetrahydrofolate + H2O = (6S)-10-formyltetrahydrofolate + H(+)
(6S)-5,6,7,8-tetrahydrofolate + ATP + formate = (6S)-10-formyltetrahydrofolate + ADP + phosphate

Subunit

Homodimer.

Similarity

In the N-terminal section; belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.
In the C-terminal section; belongs to the formate--tetrahydrofolate ligase family.

Keywords

Amino-acid biosynthesis   ATP-binding   Cytoplasm   Histidine biosynthesis   Hydrolase   Ligase   Methionine biosynthesis   Multifunctional enzyme   NADP   Nucleotide-binding   One-carbon metabolism   Oxidoreductase   Purine biosynthesis   Alternative splicing   Complete proteome   Reference proteome  

Feature

chain  C-1-tetrahydrofolate synthase, cytoplasmic
splice variant  In isoform A.

Uniprot

H9J609   A0A1S6PVE1   A0A2H1VNX3   Q27772   A0A2A4JLQ7   A0A2W1BQ31   + More
A0A2A4JL58   S4NT00   A0A194PG96   A0A194RQ72   A0A212F741   A0A2A4JKD0   A0A1J1J1D7   A0A1I8MEA8   A0A1I8MEB5   A0A1I8MEB1   A0A1W4WS48   A0A1Y1L059   A0A084W7B5   Q7QCW3   A0A182VRC4   A0A1S4FCI2   A0A182P257   A0A182UPM3   A0A182HTP7   A0A182XHP1   T1P9G4   A0A182RT97   A0A182QPE3   A0A1Q3FYF8   A0A1Q3FYD8   A0A182N758   A0A182J027   A0A182LWE5   A0A1Q3FYF0   W8BMC6   W8B1E2   A0A182GUE2   E9ILQ9   A0A067RJK1   A0A0M9A773   A0A0L7QK77   A0A0A1WE49   A0A182XW44   A0A0L0BV52   A0A232F5A3   A0A0C9QQT1   A0A1I8Q3K9   A0A1I8Q3M2   E2AG54   A0A034WE13   A0A034WFL5   A0A195DUB5   A0A0K8UA64   A0A1B6ED89   A0A026WV52   A0A1B6DLD3   A0A2M4BCH4   A0A1L8DZL7   A0A182F763   U5EUM8   A0A2M4CUR3   A0A2M4CTC1   A0A0A1WU59   A0A2M3ZFZ5   D6WUB5   A0A195CGD6   A0A195AY49   A0A0K8ULN7   A0A2M4CT95   A0A2A3EJF6   A0A088ABT0   A0A154P667   A0A2M4BCQ6   E0VA04   A0A2M4AEA5   A0A2M4AFE3   B4GLS8   A0A0R3NPL3   Q293Q3   A0A151XF40   A0A0R3NK86   B4PKR2   A0A0R1E6P9   A0A0R1E5B0   B3P1M0   O96553-2   E8NH74   O96553   A0A0B4K623   A0A0M5J4X3   B3LW32   B4HIS0   A0A1A9WGX4   A0A3B0K6U8   A0A336LX68   B4QV66   A0A1W4VZ59   A0A1B6G1K4   A0A1W4W008  

Pubmed

19121390    7893749    28756777    23622113    26354079    22118469    + More
25315136    28004739    24438588    12364791    14747013    17210077    17510324    24495485    26483478    21282665    24845553    25830018    25244985    26108605    28648823    20798317    25348373    24508170    30249741    18362917    19820115    20566863    17994087    15632085    17550304    9832531    10731132    12537572    12537569    12537568    12537573    12537574    16110336    17569856    17569867   

EMBL

BABH01029887    KY622019    AQV04396.1    ODYU01003584    SOQ42530.1    L36189    + More
NWSH01001119    PCG72504.1    KZ150014    PZC74996.1    PCG72506.1    GAIX01013842    JAA78718.1    KQ459604    KPI92322.1    KQ460045    KPJ18171.1    AGBW02009936    OWR49547.1    PCG72507.1    CVRI01000066    CRL06152.1    GEZM01071446    JAV65800.1    ATLV01021193    ATLV01021194    ATLV01021195    KE525314    KFB46109.1    AAAB01008859    EAA07766.5    APCN01000574    KA645349    AFP59978.1    AXCN02000326    GFDL01002495    JAV32550.1    GFDL01002497    JAV32548.1    AXCM01002203    GFDL01002487    JAV32558.1    GAMC01015725    GAMC01015721    JAB90834.1    GAMC01015722    GAMC01015720    JAB90833.1    JXUM01088996    JXUM01088997    JXUM01088998    JXUM01088999    KQ563739    KXJ73402.1    GL764129    EFZ18327.1    KK852643    KDR19573.1    KQ435733    KOX77269.1    KQ414983    KOC59022.1    GBXI01017018    JAC97273.1    JRES01001414    KNC23124.1    NNAY01000972    OXU25660.1    GBYB01003027    JAG72794.1    GL439266    EFN67545.1    GAKP01006023    JAC52929.1    GAKP01006022    JAC52930.1    KQ980419    KYN16129.1    GDHF01028770    JAI23544.1    GEDC01001389    JAS35909.1    KK107087    QOIP01000001    EZA59897.1    RLU27323.1    GEDC01010846    JAS26452.1    GGFJ01001613    MBW50754.1    GFDF01002217    JAV11867.1    GANO01002248    JAB57623.1    GGFL01004400    MBW68578.1    GGFL01004399    MBW68577.1    GBXI01012121    JAD02171.1    GGFM01006723    MBW27474.1    KQ971352    EFA07236.1    KQ977791    KYM99782.1    KQ976703    KYM77173.1    GDHF01024843    JAI27471.1    GGFL01004398    MBW68576.1    KZ288239    PBC31316.1    KQ434809    KZC06698.1    GGFJ01001661    MBW50802.1    DS235004    EEB10210.1    GGFK01005796    MBW39117.1    GGFK01006162    MBW39483.1    CH479185    EDW38502.1    CM000070    KRT01327.1    EAL29161.2    KQ982215    KYQ58888.1    KRT01326.1    CM000160    EDW96821.1    KRK03378.1    KRK03379.1    CH954181    EDV49619.1    AF082097    AE014297    BT021278    AY069146    BT125950    ADX35929.1    AFH06346.1    CP012526    ALC46524.1    CH902617    EDV41565.1    CH480815    EDW42717.1    OUUW01000008    SPP83790.1    UFQT01000188    SSX21369.1    CM000364    EDX13472.1    GECZ01013459    JAS56310.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000007151    UP000183832    + More
UP000095301    UP000192223    UP000030765    UP000007062    UP000075920    UP000075885    UP000075903    UP000075840    UP000076407    UP000075900    UP000075886    UP000075884    UP000075880    UP000075883    UP000069940    UP000249989    UP000027135    UP000053105    UP000053825    UP000076408    UP000037069    UP000215335    UP000095300    UP000000311    UP000078492    UP000053097    UP000279307    UP000069272    UP000007266    UP000078542    UP000078540    UP000242457    UP000005203    UP000076502    UP000009046    UP000008744    UP000001819    UP000075809    UP000002282    UP000008711    UP000000803    UP000092553    UP000007801    UP000001292    UP000091820    UP000268350    UP000000304    UP000192221   

PRIDE

H9J609    Q27772    O96553    E8NH74   

Pfam

PF00763   THF_DHG_CYH        + More
PF01268   FTHFS
PF02882   THF_DHG_CYH_C
PF01795   Methyltransf_5

Interpro

IPR036291   NAD(P)-bd_dom_sf        + More
IPR020630   THF_DH/CycHdrlase_cat_dom       
IPR000672   THF_DH/CycHdrlase       
IPR020628   Formate_THF_ligase_CS       
IPR027417   P-loop_NTPase       
IPR020867   THF_DH/CycHdrlase_CS       
IPR000559   Formate_THF_ligase       
IPR020631   THF_DH/CycHdrlase_NAD-bd_dom       
IPR002903   RsmH       
IPR023397   SAM-dep_MeTrfase_MraW_recog       
IPR029063   SAM-dependent_MTases       

SUPFAM

SSF52540   SSF52540        + More
SSF51735   SSF51735       
SSF81799   SSF81799       
SSF53335   SSF53335       

CDD

cd00477  FTHFS
cd01080  NAD_bind_m-THF_DH_Cyclohyd

ProteinModelPortal

H9J609   A0A1S6PVE1   A0A2H1VNX3   Q27772   A0A2A4JLQ7   A0A2W1BQ31   + More
A0A2A4JL58   S4NT00   A0A194PG96   A0A194RQ72   A0A212F741   A0A2A4JKD0   A0A1J1J1D7   A0A1I8MEA8   A0A1I8MEB5   A0A1I8MEB1   A0A1W4WS48   A0A1Y1L059   A0A084W7B5   Q7QCW3   A0A182VRC4   A0A1S4FCI2   A0A182P257   A0A182UPM3   A0A182HTP7   A0A182XHP1   T1P9G4   A0A182RT97   A0A182QPE3   A0A1Q3FYF8   A0A1Q3FYD8   A0A182N758   A0A182J027   A0A182LWE5   A0A1Q3FYF0   W8BMC6   W8B1E2   A0A182GUE2   E9ILQ9   A0A067RJK1   A0A0M9A773   A0A0L7QK77   A0A0A1WE49   A0A182XW44   A0A0L0BV52   A0A232F5A3   A0A0C9QQT1   A0A1I8Q3K9   A0A1I8Q3M2   E2AG54   A0A034WE13   A0A034WFL5   A0A195DUB5   A0A0K8UA64   A0A1B6ED89   A0A026WV52   A0A1B6DLD3   A0A2M4BCH4   A0A1L8DZL7   A0A182F763   U5EUM8   A0A2M4CUR3   A0A2M4CTC1   A0A0A1WU59   A0A2M3ZFZ5   D6WUB5   A0A195CGD6   A0A195AY49   A0A0K8ULN7   A0A2M4CT95   A0A2A3EJF6   A0A088ABT0   A0A154P667   A0A2M4BCQ6   E0VA04   A0A2M4AEA5   A0A2M4AFE3   B4GLS8   A0A0R3NPL3   Q293Q3   A0A151XF40   A0A0R3NK86   B4PKR2   A0A0R1E6P9   A0A0R1E5B0   B3P1M0   O96553-2   E8NH74   O96553   A0A0B4K623   A0A0M5J4X3   B3LW32   B4HIS0   A0A1A9WGX4   A0A3B0K6U8   A0A336LX68   B4QV66   A0A1W4VZ59   A0A1B6G1K4   A0A1W4W008  

PDB

4JKI     E-value=2.34062e-139,     Score=1274

KEGG

101743494    K00288 methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / f

PATHWAY

00670    One carbon pool by folate - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0004488   GO:0004329   GO:0005524   GO:0006164   GO:0005737   GO:0009086   GO:0000105   GO:0035999   GO:0004477   GO:0009113   GO:0009257   GO:0016787   GO:0008168   GO:0008652   GO:0003824   GO:0009396   GO:0031167   GO:0003677   GO:0008408   GO:0005112   GO:0015930   GO:0055114  

Subcellular location

Cytoplasm  

Length:

972

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.50644

Exp number, first 60 AAs:

0.00109

Total prob of N-in:

0.00055

outside

1  -  972

 

 

Pi

271.465554

Theta

185.604193

Tajima's D

1.752803

CLR

0.185425

CSRT

0.84120793960302

Interpretation

Uncertain