Gene

KWMTBOMO15171  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004979

Annotation

PREDICTED:_diphthine_synthase_[Plutella_xylostella]

Location

Bomo_Chr25(-):6082369-6090613      View in EpiBrowser

Full name

Diphthine methyl ester synthase      

Alternative Name

Diphthamide biosynthesis methyltransferase

Location in the cell

Cytoplasmic   Reliability : 3.228

CDS

ATGTTTTATTTAATTGGGTTAGGTCTTGGCGACGCAAAAGATATTACAGTTAAAGGCTTAGAAATAGTAAAGAAATGTGATAAAGTGTATCTAGAGGCATATACTTCAATATTAACTATCGGAAAAGAAGCCTTAGAAACATTTTATGGGAGAACATTAACATTAGCTGACCGTGAACTATGTGAAAGCAATATAGATGACATCCTCAAAGAGGCTAAAGATATTGAAATTGCATTATTAGTAGTTGGCGATCCTCTTGGGGCTACAACACATACCGATATGTTGCTTCGTGCAAAAGAATTGGGAGTTAAAACACAGATAGTACACAATGCATCAATTATGAATGCGGTCAGTTGCTGTGGATTGCAATTATATAACTTTGGAGAAACTGTATCAATACCCTTCTGGACTGATACATGGAAACCTGATAGCTTTTTCGAAAAAATTATAGCTAATTACTCAAGAAATCTCCATACTTTATGTCTTTTAGATATCAAAGTTAAGGAGCCCACAGAGGAATCCCTGACCAAGAAGGTGAGAACTTACATGGATCCAAAATTTATGTCTGTTAAAGATGCTGCAATACAGCTGGTGCAGATCATTGATGCTAAGCCTGATTGTGGTATACAACGTGATTGCACAGCAGTGGGCCTTTCAAGGGTGGGAGCGCCTGACCAAAGGATATCTGTTATGACACTGGAAGCTATGCAGATGTCGGACCTCGGGCCGCCTCTGCATAGCCTCGTCATCCCCGCTCCCAATTTACATCCGCTCGAACTAGACTATCTTTCACAATTTAGATAG

Protein

MFYLIGLGLGDAKDITVKGLEIVKKCDKVYLEAYTSILTIGKEALETFYGRTLTLADRELCESNIDDILKEAKDIEIALLVVGDPLGATTHTDMLLRAKELGVKTQIVHNASIMNAVSCCGLQLYNFGETVSIPFWTDTWKPDSFFEKIIANYSRNLHTLCLLDIKVKEPTEESLTKKVRTYMDPKFMSVKDAAIQLVQIIDAKPDCGIQRDCTAVGLSRVGAPDQRISVMTLEAMQMSDLGPPLHSLVIPAPNLHPLELDYLSQFR

Description

S-adenosyl-L-methionine-dependent methyltransferase that catalyzes four methylations of the modified target histidine residue in translation elongation factor 2 (EF-2), to form an intermediate called diphthine methyl ester. The four successive methylation reactions represent the second step of diphthamide biosynthesis.

Catalytic Activity

2-[(3S)-amino-3-carboxypropyl]-L-histidyl-[translation elongation factor 2] + 4 S-adenosyl-L-methionine = diphthine methyl ester-[translation elongation factor 2] + 3 H(+) + 4 S-adenosyl-L-homocysteine

Similarity

Belongs to the diphthine synthase family.

Keywords

Complete proteome   Methyltransferase   Phosphoprotein   Reference proteome   S-adenosyl-L-methionine   Transferase  

Feature

chain  Diphthine methyl ester synthase

Uniprot

H9J638   A0A2A4JN23   A0A194PG66   A0A2H1WWW2   S4Q0C8   A0A194RLA3   + More
A0A212ENP1   A0A1E1WKF0   A0A0T6B0U8   A0A0B4J2S3   A0A0C9PHJ3   A0A0C9QXA2   B0WNF9   A0A1Q3F2I8   A0A182KZR6   Q7QA67   A0A182HVP2   A0A182XF71   A0A0J7KDU5   A0A232EMI4   A0A182THH9   A0A1Y1MLG7   A0A182IJF8   A0A182UUW4   A0A182SEX1   E1ZXL1   A0A182RI21   A0A026WP88   A0A1W4WYI7   U4UJ53   A0A182PLH5   N6TI26   E2BLJ6   A0A1B0CXD5   D6WME0   A0A182MK68   A0A182NCV3   A0A2M4BW35   A0A158P049   A0A182Y904   A0A195E027   A0A195BGM3   F4X3U1   A0A1B0D3X0   A0A2J7R4R6   A0A2M4AYT4   A0A151XJK2   A0A2M3ZJ90   A0A182W388   A0A2M3ZBK8   A0A1B3PDM0   A0A067R3Q3   A0A182Q3G7   A0A2M4CUQ5   E9HLA8   H0VAJ6   A0A182GQF1   A0A091D8S2   T1HFE9   Q17H41   A0A0P5ESH9   A0A162NX87   A0A210R1M7   A0A0P6HL29   A0A1W4W2D8   A0A2Y9IXP4   A0A0V0GCU4   A0A2U3X9T0   A0A2U3X9W9   A0A2K6EMJ7   A0A1B0G0N5   A0A1A9XN10   A0A1A9UZS7   A0A1B0C2U5   A0A1A9ZZP7   M1ELR5   Q9CWQ0   M3YKW4   B4GP41   A0A3B0KB11   Q569A7   A0A2I2UH15   Q29BK7   B4M179   B4HEQ0   L5K5S4   A0A1A9W6M8   A0A0A9XCS8   B4K7P6   B3M004   E2RQL8   B4R0M6   B4PMB9   B3P8N0   W8BWC3   A0A3Q7TB74   U6CN03   E0V961   A0A2Y9H8Y1   B4NAV5  

EC Number

2.1.1.314

Pubmed

19121390    26354079    23622113    22118469    20075255    20966253    + More
12364791    14747013    17210077    28648823    28004739    20798317    24508170    30249741    23537049    18362917    19820115    21347285    25244985    21719571    24845553    21292972    21993624    26483478    17510324    28812685    23236062    16141072    19468303    15489334    15485916    21183079    17994087    15057822    15632090    17975172    15632085    23258410    25401762    26823975    16341006    17550304    24495485    20566863   

EMBL

BABH01029937    BABH01029938    NWSH01001069    PCG72800.1    KQ459604    KPI92292.1    + More
ODYU01011623    SOQ57482.1    GAIX01000013    JAA92547.1    KQ460045    KPJ18199.1    AGBW02013634    OWR43077.1    GDQN01003554    JAT87500.1    LJIG01016323    KRT80946.1    GBYB01000303    JAG70070.1    GBYB01000305    JAG70072.1    DS232010    EDS31613.1    GFDL01013337    JAV21708.1    AAAB01008898    EAA09127.4    APCN01005809    LBMM01008815    KMQ88618.1    NNAY01003348    OXU19573.1    GEZM01028008    JAV86521.1    GL435066    EFN74094.1    KK107139    QOIP01000003    EZA57872.1    RLU24634.1    KB632197    ERL89990.1    APGK01036914    KB740945    ENN77473.1    GL449033    EFN83433.1    AJWK01033613    KQ971343    EFA04256.2    AXCM01001830    GGFJ01008146    MBW57287.1    ADTU01005357    KQ979955    KYN18456.1    KQ976490    KYM83371.1    GL888624    EGI58888.1    AJVK01011076    NEVH01007402    PNF35828.1    GGFK01012623    MBW45944.1    KQ982074    KYQ60521.1    GGFM01007784    MBW28535.1    GGFM01005130    MBW25881.1    KU660012    AOG17810.1    KK852718    KDR17798.1    AXCN02001207    GGFL01004899    MBW69077.1    GL732677    EFX67483.1    AAKN02004287    AAKN02004288    JXUM01080397    KQ563183    KXJ74317.1    KN123067    KFO26893.1    ACPB03004962    CH477253    EAT45972.1    GDIQ01265348    JAJ86376.1    LRGB01000512    KZS18345.1    NEDP02000888    OWF54781.1    GDIQ01017446    JAN77291.1    GECL01000220    JAP05904.1    CCAG010015463    JXJN01024713    JP008922    AER97519.1    AK010475    AC131113    CH466532    BC060372    AEYP01085866    AEYP01085867    AEYP01085868    AEYP01085869    AEYP01085870    CH479186    EDW38924.1    OUUW01000005    SPP80758.1    AABR07013018    AABR07013019    BC092598    CH473952    AAH92598.1    EDL82019.1    AANG04003279    CM000070    EAL26990.2    CH940650    EDW67490.1    CH480815    EDW43211.1    KB031037    ELK05833.1    GBHO01028705    GBHO01028704    GDHC01017189    GDHC01012101    JAG14899.1    JAG14900.1    JAQ01440.1    JAQ06528.1    CH933806    EDW16417.1    CH902617    EDV44194.1    AAEX03004766    CM000364    EDX13949.1    CM000160    EDW98024.1    CH954182    EDV54125.1    GAMC01003038    JAC03518.1    HAAF01000079    CCP71905.1    DS234988    EEB09917.1    CH964232    EDW80919.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000007151    UP000002358    + More
UP000002320    UP000075882    UP000007062    UP000075840    UP000076407    UP000036403    UP000215335    UP000075902    UP000075880    UP000075903    UP000075901    UP000000311    UP000075900    UP000053097    UP000279307    UP000192223    UP000030742    UP000075885    UP000019118    UP000008237    UP000092461    UP000007266    UP000075883    UP000075884    UP000005205    UP000076408    UP000078492    UP000078540    UP000007755    UP000092462    UP000235965    UP000075809    UP000075920    UP000027135    UP000075886    UP000000305    UP000005447    UP000069940    UP000249989    UP000028990    UP000015103    UP000008820    UP000076858    UP000242188    UP000192221    UP000248482    UP000245341    UP000233160    UP000092444    UP000092443    UP000078200    UP000092460    UP000092445    UP000000589    UP000000715    UP000008744    UP000268350    UP000002494    UP000011712    UP000001819    UP000008792    UP000001292    UP000010552    UP000091820    UP000009192    UP000007801    UP000002254    UP000000304    UP000002282    UP000008711    UP000286640    UP000009046    UP000248481    UP000007798   

PRIDE

H9J638    Q9CWQ0   

Pfam

PF00590   TP_methylase

Interpro

IPR014776   4pyrrole_Mease_sub2        + More
IPR004551   Dphthn_synthase       
IPR035996   4pyrrol_Methylase_sf       
IPR000878   4pyrrol_Mease       
IPR014777   4pyrrole_Mease_sub1       

SUPFAM

SSF53790   SSF53790       

Gene 3D

3.30.950.10     3.40.1010.10    

CDD

cd11647  Diphthine_synthase

ProteinModelPortal

H9J638   A0A2A4JN23   A0A194PG66   A0A2H1WWW2   S4Q0C8   A0A194RLA3   + More
A0A212ENP1   A0A1E1WKF0   A0A0T6B0U8   A0A0B4J2S3   A0A0C9PHJ3   A0A0C9QXA2   B0WNF9   A0A1Q3F2I8   A0A182KZR6   Q7QA67   A0A182HVP2   A0A182XF71   A0A0J7KDU5   A0A232EMI4   A0A182THH9   A0A1Y1MLG7   A0A182IJF8   A0A182UUW4   A0A182SEX1   E1ZXL1   A0A182RI21   A0A026WP88   A0A1W4WYI7   U4UJ53   A0A182PLH5   N6TI26   E2BLJ6   A0A1B0CXD5   D6WME0   A0A182MK68   A0A182NCV3   A0A2M4BW35   A0A158P049   A0A182Y904   A0A195E027   A0A195BGM3   F4X3U1   A0A1B0D3X0   A0A2J7R4R6   A0A2M4AYT4   A0A151XJK2   A0A2M3ZJ90   A0A182W388   A0A2M3ZBK8   A0A1B3PDM0   A0A067R3Q3   A0A182Q3G7   A0A2M4CUQ5   E9HLA8   H0VAJ6   A0A182GQF1   A0A091D8S2   T1HFE9   Q17H41   A0A0P5ESH9   A0A162NX87   A0A210R1M7   A0A0P6HL29   A0A1W4W2D8   A0A2Y9IXP4   A0A0V0GCU4   A0A2U3X9T0   A0A2U3X9W9   A0A2K6EMJ7   A0A1B0G0N5   A0A1A9XN10   A0A1A9UZS7   A0A1B0C2U5   A0A1A9ZZP7   M1ELR5   Q9CWQ0   M3YKW4   B4GP41   A0A3B0KB11   Q569A7   A0A2I2UH15   Q29BK7   B4M179   B4HEQ0   L5K5S4   A0A1A9W6M8   A0A0A9XCS8   B4K7P6   B3M004   E2RQL8   B4R0M6   B4PMB9   B3P8N0   W8BWC3   A0A3Q7TB74   U6CN03   E0V961   A0A2Y9H8Y1   B4NAV5  

PDB

3I4T     E-value=2.53999e-69,     Score=664

KEGG

101746700    K00586 diphthine methyl ester synthase [EC:2.1.1.314] | (RefSeq) diphthine synthase

GO

GO:0004164   GO:0017183   GO:0008152   GO:0008168   GO:0006391   GO:0031167   GO:0003677   GO:0008408   GO:0005112   GO:0015930  

PANTHER

PTHR10882:SF0  

Length:

267

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00549

Exp number, first 60 AAs:

0.00021

Total prob of N-in:

0.00226

outside

1  -  267

 

 

Pi

205.156236

Theta

152.866097

Tajima's D

1.064932

CLR

0.481763

CSRT

0.680265986700665

Interpretation

Uncertain