Gene

KWMTBOMO15138

Pre Gene Modal

BGIBMGA004921

Annotation

PREDICTED:_4-coumarate--CoA_ligase_1-like_[Bombyx_mori]

Location

Bomo_Chr25(+):5727875-5734699      View in EpiBrowser

Full name

Luciferin 4-monooxygenase      

Location in the cell

Cytoplasmic   Reliability : 2.927

CDS

ATGGTGTACTCACCTGTAGATTACTTAAAAGCCATTGAGGAATATAAGGTAAACCTTTTGATAATAGTGCCGCCAATCGCTGTTTTTCTATCAAAGTCTGATCTCGTCCAAAAGTATGACTTGTCTTCAGTGACTAGAATTTGGTGTGGTGCAGCTCCACTCAGTCAAGAGACGACTGTGGGGGTTTTAAGGAATTTCCCTAACTGTCAGAAGATAATCCATGGATACGGAATGACGGAAACATCTTTTATAATAATAAAAAGCTATGATACCTTGGACAGCGAACAAAAGCATGGAAGTTGCGGGCACGTCGTACCTGGCATGAAAGTGAAGGTTGTTGACATTAATACTCGACAAACATTAGGATCGTATGAACGAGGTGAAGTCTGCGTTAAAGGACCCCTGGTGATGAAAGGCTACATGAATAACGATCAAGCTAATGTAGATATAAGGGACGACGAGGGTTTCCTCAAAACTGGAGATATCGGATATTATGATTCAGACGGCTGTTTCTACATAGTCGACAGACTGAAAGAACTTATCAAATACAAAGGACATCAGGTAGCTCCGGCAATGATAGAGCGCGTACTGCTGCAGCACAGCGACGTGTTGGAGTGCGGAGTGGTGGGAGCGCCCGACGAGTTAGCTGGCGAGCTGCCCACCGCCTTCGTAGTGCCCAAGCCTGACGTCACGCTCACAGAAAAAGAACTGTTAGACTTTACTAATGAACGGGTCGGTAACATAGTTTGA

Protein

MVYSPVDYLKAIEEYKVNLLIIVPPIAVFLSKSDLVQKYDLSSVTRIWCGAAPLSQETTVGVLRNFPNCQKIIHGYGMTETSFIIIKSYDTLDSEQKHGSCGHVVPGMKVKVVDINTRQTLGSYERGEVCVKGPLVMKGYMNNDQANVDIRDDEGFLKTGDIGYYDSDGCFYIVDRLKELIKYKGHQVAPAMIERVLLQHSDVLECGVVGAPDELAGELPTAFVVPKPDVTLTEKELLDFTNERVGNIV

Description

Produces green light.
Produces green light with a wavelength of 544 nm.
Produces green light with a wavelength of 570 nm.

Catalytic Activity

ATP + firefly D-luciferin + O2 = AMP + CO2 + diphosphate + firefly oxyluciferin + hnu

Cofactor

Mg(2+)

Biophysicochemical Properties

11.5 uM for luciferin
130 uM for ATP

Subunit

Homodimer.

Similarity

Belongs to the ATP-dependent AMP-binding enzyme family.

Keywords

ATP-binding   Luminescence   Magnesium   Metal-binding   Monooxygenase   Nucleotide-binding   Oxidoreductase   Peroxisome   Photoprotein   3D-structure  

Feature

chain  Luciferin 4-monooxygenase

Uniprot

H9J5Y0   H9J5X9   A0A2A4JN67   A0A2A4JMM7   A0A194PHR8   A0A194RK67   + More
A0A3S2NFX7   A0A2H1WNI5   A0A2A4JNM0   A0A194PGU6   A0A194RLN8   A0A3S2PFN8   H9J647   A0A1Y9H2Q7   A0A2C9GUN5   A0A240PK82   A0A182MLJ2   Q2ACC9   A0A1Y9IUV9   H9J2F6   A0A1I8JUE9   A0A194PT23   G8GE17   A0A182Q5M2   I4DPZ6   A0A212ETT6   A0A240PLR9   A0A182R329   A0A182TA88   A0A182YM73   Q1WLP6   A0A1I8JUI2   A0A194QZM0   A0A182TRT7   A0A182LYC6   A0A0J7KT57   A0A182Q9Y0   A0A2J7QLT8   A0A1I8JVD9   A0A1I8JTE0   F5HJ76   A0A084VAF1   A0A182WW21   K7J1G6   A0A182JAX9   A0A1Y9H2R4   Q8ITG4   Q8T6U3   A0A1Q2TT53   Q8ITG5   Q8I0E8   A0A2J7QLX8   H1AD94   Q01158   H1AD95   E3WI50   A0A1Q2TT48   P13129   Q2ACC8   Q27348   A0A182T271   Q2ABY2   A0A084VAF2   A0A0C6DRW5   A0A2W1BTP3   A0A182YH13   A0A182W5X3   A0A1Y9IV15   A0A182YM74   Q27321   Q8IRZ9   Q25118   Q8ITG3   E2AA43   A0A232FI37   Q9U4U7   D2DHG8   E3SH50   Q26304   E0VSL5   A0A1I8JVG1   Q1AG35   B3TMS5   A0A084W803   A0A2M4CMC1   A0A182QSE0   A0A1I8JTD3   A0A2M4BJS1   A0A2M4BJM2   A0A2M4BJN0   D2DHG9   A0A240PNB0   Q5TVT3   A0A067R342   G9M6I9   A0A1I8JVV1   A0A2S3QZT6   A0A182F8R8   Q05KC0  

EC Number

1.13.12.7

Pubmed

19121390    26354079    16380223    22651552    22118469    25244985    + More
17002879    12364791    14747013    17210077    24438588    20075255    12568798    12091112    1610896    2473944    10380103    28756777    7480137    20798317    28648823    10387072    30456363    8504162    2801227    20566863    16916615    24845553    29358930   

EMBL

BABH01029976    BABH01029979    BABH01029980    NWSH01001030    PCG73034.1    PCG73036.1    + More
KQ459604    KPI92264.1    KQ460045    KPJ18228.1    RSAL01000053    RVE50075.1    ODYU01009511    SOQ54004.1    PCG73033.1    KPI92263.1    KPJ18230.1    RVE50074.1    BABH01029982    AXCM01004086    AB196455    BAE80728.1    BABH01007891    KQ459593    KPI96566.1    JN127350    AET05796.1    AXCN02000090    AK403861    BAM19986.1    AGBW02012531    OWR44889.1    DQ137139    AAZ74651.1    KQ460890    KPJ10988.1    AXCM01007305    AXCM01007306    LBMM01003413    KMQ93562.1    NEVH01013241    PNF29543.1    APCN01002222    AAAB01008820    EGK96337.1    ATLV01003206    KE524124    KFB34945.1    AF486801    AAN40976.1    AF420006    AAM00429.1    AB909130    BAW94791.1    AF486800    AAN40975.1    AF486802    AF486803    AAN40977.1    PNF29548.1    AB644226    BAL46510.1    X66919    AB644227    BAL46511.1    AB603803    BAJ41367.1    AB908263    BAW94790.1    M26194    AB196456    BAE80729.1    U49182    AY192561    Z49891    AAA91472.1    AAO39674.1    CAA90072.1    AB220162    BAE80731.1    KFB34946.1    AB251887    BAQ25864.1    KZ149903    PZC78438.1    U49181    U51019    Z69619    AAA91471.1    AAB00229.1    CAA93444.1    AY181996    AY192560    AAN73267.1    AAO39673.2    L39929    AAC37253.1    AF486804    AAN40979.1    GL438029    EFN69681.1    NNAY01000193    OXU30119.1    AF139645    AAD34543.2    FJ545728    ACT68596.1    GU062703    ADK56478.1    S61961    DS235751    EEB16371.1    DQ138966    ABA03040.1    EU302126    ABZ88151.1    ATLV01021336    KE525316    KFB46347.1    GGFL01002163    MBW66341.1    GGFJ01004113    MBW53254.1    GGFJ01004114    MBW53255.1    GGFJ01004116    MBW53257.1    FJ545729    ACT68597.1    EAL41501.4    KK852730    KDR17523.1    AB604790    BAL40875.1    PDGH01000119    POB45175.1    AB255748    BAF34360.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000283053    UP000075884    + More
UP000075883    UP000075885    UP000075920    UP000075900    UP000075886    UP000007151    UP000075881    UP000075901    UP000076408    UP000075902    UP000036403    UP000235965    UP000075903    UP000075840    UP000007062    UP000030765    UP000076407    UP000002358    UP000075880    UP000000311    UP000215335    UP000009046    UP000027135    UP000237466    UP000069272   

PRIDE

H9J5Y0    H9J5X9    H9J647    H9J2F6    G8GE17    Q26304    + More

Pfam

PF00501   AMP-binding        + More
PF13193   AMP-binding_C

Interpro

IPR020845   AMP-binding_CS        + More
IPR042099   AMP-dep_Synthh-like_sf       
IPR000873   AMP-dep_Synth/Lig       
IPR025110   AMP-bd_C       
IPR027380   Luciferase-like_N_sf       

Gene 3D

3.40.50.12780     4.10.8.10    

ProteinModelPortal

H9J5Y0   H9J5X9   A0A2A4JN67   A0A2A4JMM7   A0A194PHR8   A0A194RK67   + More
A0A3S2NFX7   A0A2H1WNI5   A0A2A4JNM0   A0A194PGU6   A0A194RLN8   A0A3S2PFN8   H9J647   A0A1Y9H2Q7   A0A2C9GUN5   A0A240PK82   A0A182MLJ2   Q2ACC9   A0A1Y9IUV9   H9J2F6   A0A1I8JUE9   A0A194PT23   G8GE17   A0A182Q5M2   I4DPZ6   A0A212ETT6   A0A240PLR9   A0A182R329   A0A182TA88   A0A182YM73   Q1WLP6   A0A1I8JUI2   A0A194QZM0   A0A182TRT7   A0A182LYC6   A0A0J7KT57   A0A182Q9Y0   A0A2J7QLT8   A0A1I8JVD9   A0A1I8JTE0   F5HJ76   A0A084VAF1   A0A182WW21   K7J1G6   A0A182JAX9   A0A1Y9H2R4   Q8ITG4   Q8T6U3   A0A1Q2TT53   Q8ITG5   Q8I0E8   A0A2J7QLX8   H1AD94   Q01158   H1AD95   E3WI50   A0A1Q2TT48   P13129   Q2ACC8   Q27348   A0A182T271   Q2ABY2   A0A084VAF2   A0A0C6DRW5   A0A2W1BTP3   A0A182YH13   A0A182W5X3   A0A1Y9IV15   A0A182YM74   Q27321   Q8IRZ9   Q25118   Q8ITG3   E2AA43   A0A232FI37   Q9U4U7   D2DHG8   E3SH50   Q26304   E0VSL5   A0A1I8JVG1   Q1AG35   B3TMS5   A0A084W803   A0A2M4CMC1   A0A182QSE0   A0A1I8JTD3   A0A2M4BJS1   A0A2M4BJM2   A0A2M4BJN0   D2DHG9   A0A240PNB0   Q5TVT3   A0A067R342   G9M6I9   A0A1I8JVV1   A0A2S3QZT6   A0A182F8R8   Q05KC0  

PDB

6AC3     E-value=1.55295e-62,     Score=605

KEGG

101745810    no KO assigned | (RefSeq) 4-coumarate--CoA ligase 1-like

GO

GO:0003824   GO:0004497   GO:0016021   GO:0016874   GO:0047077   GO:0008218   GO:0005777   GO:0005524   GO:0046872   GO:0000287   GO:0008152   GO:0003677   GO:0046983   GO:0005525   GO:0000166   GO:0008408   GO:0005112   GO:0015930  

Subcellular location

Peroxisome  

Length:

249

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.18782

Exp number, first 60 AAs:

0.18544

Total prob of N-in:

0.04645

outside

1  -  249

 

 

Pi

155.482437

Theta

163.166763

Tajima's D

0.377845

CLR

0.269612

CSRT

0.476626168691565

Interpretation

Uncertain