Gene

KWMTBOMO15082

Annotation

unnamed_protein_product_[Drosophila_melanogaster]

Location

Bomo_Chr25(+):4505432-4508346      View in EpiBrowser

Full name

RNA-directed DNA polymerase from mobile element jockey       + More
Probable RNA-directed DNA polymerase from transposon BS      

Alternative Name

Reverse transcriptase

Location in the cell

Nuclear   Reliability : 2.795

CDS

ATGACTGCACTAAAAATAGCTACATGGAACATCAATGGCTTGGCACCCAACATCGATGAAGTGGAAATTCTGATCTGTCTACATAGTCTAGACATTCTCCTAATTTCAGAGACACATATGACGACCAGCAGCTCGATAAGAATTAAAGGATATAAGATCTATTCCACAAATCACCCAGACGGTACAGCCCACGGCGGGACAGCAGTGATAATCAAAGCCACTGTTAGACACCATGTGCTACCAAACTACTGTACCGAACATATCCAGGCTACCACAGTAGCAGTCAGTGACAGAAATGGAGACTTTAATATATCATCAATTTATTGTCCCCCGAAACATAAAATAAGTGAAGCACAGTTTTCTGACTACTTTTTAAGCTTAGGATACAGATTTATTGCTGGGGGTGACTGGAATGCGAAGCATTCGCACTGGGGCTCTAGATTAATAACTACACGTGGAAGGGAGCTGAAGAAAAGTGTAGATAAACAGTTCTTGACTACTGTGTCAACTCCAGAACCAACACACTGGCCCACAGACCAGAACAGACTGCCAGACGTTCTAGACTTTTTCATCACAACAGGACTGTCTCGACTGTTCTTCAATGTTGAGTCATGCCTCGATAGCTCATCTAACCATATTCCTGTAATATTGACAGTGAGCACCACTATTCTTAAGAAAGAAGTTCGGCAAACATTGTACAACAAATACACAGACTGGGATAGCTTCCGGGAAATAGTTGATACGAAGCTAAGCCTAAAAATAGCACTTAAAACAGAAGAGGATATCGAAGAAGCCACAAAGCATTTCACCACAACAGTTCAAGCTGCCTGCTGGAAAACCTCTCCGGAGCACTTGAATCCCAGGCCACGGCAAAATAAGATATCGTTTGAAATAAAAGAACTGATTCTGAACAAAAGAAAACTGCGAAGAAATAACACTTGGGCAAGAACAAGAAAAGAAAAAGCTGACGCCTTTGCTGTTCACTTAGCAAAAGTATTCACGCCTAATGATGCTGACCCAAACACTGATGATTCCTTCATAGATTCGGCCCTAAGTCAAGACTTCCAGCTCGATTTGCCGATTAGGCCTACAACTCCGAAAGAAGTCGTGGCAACAATCTCAAAATTGGAGGACAAAAAAGCCCCCGGGTTTGATTTAATAGACAAAAAAGTCTTGAAGGAACTTCCCAAAAAAGCCATAGTTTTTCTAACCATCTTATTTAATGGCATACTAAGAACTGGACACTTTCCATCACTGTGGAGAGTTTCACAAGTAATTATGATACCTAAAGTAGGGAAGCCAGTGAACGAACTTAGCTCATACAGGCCGATTAGTCTACTAACCGTAACGTCAAAACTTTTTGAGTCAGTCCTGCTGCACAGATTATTGGAGGAGATTCATCAAAGAGCCATAATCCCGGACCACCAGTTTGGCTTTCGCCAGCAGCATGGCACTGTGGAACAAGTCCATAGAATCTGCTCTGTGATAAGGAAGGCACTAGAGAGAAAGGAGTACTGCTCCTCTGCCTTCCTAGACGTGCAGCAAGCGTTTGATAGAGTATGGCACAAAGGTCTCTTGTATAAAGTAAAAACTCTTCTACCCTGTACATTCTTCCCCATCATAAAATCTTATCTAACAGAAAGAATATTTCAAATAAGAGAAGAAGAGTGTACATCGCCATTTCACTCAATCAAAGCTGGTGTACCGCAGGGGTCTGTCCTGGGTCCAGTCTTGTATACCATTTACACATGTGATTTACCACAAAGCGAACATGTTACATTAGCAACATTCGCAGACGATACCGCTGTACTGGCATGTAGCAATTGCCCAAACGAAGCTTCAGACTTCCTACAGACTGGATTGCAAGAAATTGAAACTTGGCTTTCAAAGTGGAGAATAAAAACTAGTGTGACAAAATCCGTTCATGTAACCTTCACCCTCCGTAGAGGAGACTGTCCGCCAGTAACTCTGTGCAATCAACAACTCCCGCAAGAACACTGCGTAAAATACTTGGGAATACACCTTGATAGGAGACTCGTATGGAAACAACACATACAGAAGAAGAGAGAAGAAATCAACCTGAAATACAGAGGACTCTACTGGCTGCTTGGCAGAAATTCCAAGCTGTCAGTAGATAATAAGCTACTCATCTACGAAATGCTCTTGAAACCAATTTGGACATACGGGATTCAACTATGGGGTTCGGCGTGTGACTCTAACATAAATATCATACAGAGGGTTCAAAATTACATCCTGAAACAAATATCGAACTGTCCGTGGTTTATAAAAACATCGGAGATTCATGAACACCTCAAAATCAGGACTGTTAAACAGGAAATCAAGACCTGGAGTATGAAATACAGAAAAAGGCTAGAAATGCATCCTAATAAGCTTGCCACGCAACTAACCACACCGGACCTAATAAATAGGCTTAAAAGGCGACAAATCCTAAGTCTTGATAGAAACGGTTGA

Protein

MTALKIATWNINGLAPNIDEVEILICLHSLDILLISETHMTTSSSIRIKGYKIYSTNHPDGTAHGGTAVIIKATVRHHVLPNYCTEHIQATTVAVSDRNGDFNISSIYCPPKHKISEAQFSDYFLSLGYRFIAGGDWNAKHSHWGSRLITTRGRELKKSVDKQFLTTVSTPEPTHWPTDQNRLPDVLDFFITTGLSRLFFNVESCLDSSSNHIPVILTVSTTILKKEVRQTLYNKYTDWDSFREIVDTKLSLKIALKTEEDIEEATKHFTTTVQAACWKTSPEHLNPRPRQNKISFEIKELILNKRKLRRNNTWARTRKEKADAFAVHLAKVFTPNDADPNTDDSFIDSALSQDFQLDLPIRPTTPKEVVATISKLEDKKAPGFDLIDKKVLKELPKKAIVFLTILFNGILRTGHFPSLWRVSQVIMIPKVGKPVNELSSYRPISLLTVTSKLFESVLLHRLLEEIHQRAIIPDHQFGFRQQHGTVEQVHRICSVIRKALERKEYCSSAFLDVQQAFDRVWHKGLLYKVKTLLPCTFFPIIKSYLTERIFQIREEECTSPFHSIKAGVPQGSVLGPVLYTIYTCDLPQSEHVTLATFADDTAVLACSNCPNEASDFLQTGLQEIETWLSKWRIKTSVTKSVHVTFTLRRGDCPPVTLCNQQLPQEHCVKYLGIHLDRRLVWKQHIQKKREEINLKYRGLYWLLGRNSKLSVDNKLLIYEMLLKPIWTYGIQLWGSACDSNINIIQRVQNYILKQISNCPWFIKTSEIHEHLKIRTVKQEIKTWSMKYRKRLEMHPNKLATQLTTPDLINRLKRRQILSLDRNG

Catalytic Activity

a 2'-deoxyribonucleoside 5'-triphosphate + DNA(n) = diphosphate + DNA(n+1)

Cofactor

Mg(2+)
Mn(2+)

Miscellaneous

Prefers poly(rC) and poly(rA) as template and activated DNA is not effective.

Keywords

Nucleotidyltransferase   RNA-directed DNA polymerase   Transferase   Transposable element  

Feature

chain  RNA-directed DNA polymerase from mobile element jockey

Uniprot

A0A2A4IUM2   A0A2J7R579   A0A2J7R4B1   A0A2J7PVX3   A0A2J7QPI2   A0A034VFD2   + More
A0A142LX26   A0A1Y1LKN6   A0A224X6T4   Q04135   Q6R811   A0A2S2N814   A0A224XJI0   J9LYS0   A0A2S2PHL7   A0A224X746   A0A224X6U5   A0A224XIB0   A0A2H8TKI9   X1WUG2   A0A0J7K7J8   A0A2S2P698   A0A352X8G4   A0A2S2NGV8   A0A2S2NKC3   A0A224XI99   A0A023F0I3   A0A2S2P3X8   A0A2H8TF37   A0A2S2PJG2   A0A2S2P6S3   A0A2S2P6C4   A0A2S2NH02   A0A142LX53   A0A2S2QDN9   X1WIX8   A0A2S2PUS3   A0A0J7K811   J9KWJ0   A0A2S2QEE0   A0A2S2PA10   A0A0J7KC92   J9KE07   A0A2S2Q2R8   A0A2S2N9P4   J9M0H4   P21328   A0A1Y1MT28   P21329   Q95SX7   A0A2S2Q7E7   A0A2S2PBB4   A0A2S2NI07   A0A2S2PX53   J9LBF1   A0A2S2PPK8  

EC Number

2.7.7.49

Pubmed

25348373    25527032    28004739    1705654    14745026    30148503    + More
25474469    2463954    3040362    1714378    1701254    7753626    12537569   

EMBL

NWSH01007753    PCG62823.1    NEVH01007393    PNF35987.1    NEVH01007578    PNF35646.1    + More
NEVH01020940    PNF20481.1    NEVH01012089    PNF30491.1    GAKP01017773    JAC41179.1    KU543673    AMS38352.1    GEZM01053022    JAV74219.1    GFTR01008261    JAW08165.1    X17551    CAA35587.1    AY508487    AAS13459.1    GGMR01000676    MBY13295.1    GFTR01008257    JAW08169.1    ABLF02041861    GGMR01016296    MBY28915.1    GFTR01008151    JAW08275.1    GFTR01008251    JAW08175.1    GFTR01008246    JAW08180.1    GFXV01002840    MBW14645.1    ABLF02016613    LBMM01012041    KMQ86458.1    GGMR01012305    MBY24924.1    DNOY01000259    HBE18313.1    GGMR01003407    MBY16026.1    GGMR01005022    MBY17641.1    GFTR01008256    JAW08170.1    GBBI01003747    JAC14965.1    GGMR01011511    MBY24130.1    GFXV01000908    MBW12713.1    GGMR01016905    MBY29524.1    GGMR01012443    MBY25062.1    GGMR01012283    MBY24902.1    GGMR01003864    MBY16483.1    KU543687    AMS38379.1    GGMS01006655    MBY75858.1    ABLF02017874    ABLF02027031    GGMR01020516    MBY33135.1    LBMM01012078    KMQ86419.1    ABLF02021819    ABLF02021820    ABLF02055326    GGMS01006359    MBY75562.1    GGMR01013409    MBY26028.1    LBMM01009710    KMQ87932.1    ABLF02024784    ABLF02024792    GGMS01002786    MBY71989.1    GGMR01000857    MBY13476.1    ABLF02034460    M22874    M38643    GEZM01021881    JAV88842.1    M38437    AY060438    AAL25477.1    GGMS01004444    MBY73647.1    GGMR01014053    MBY26672.1    GGMR01003787    MBY16406.1    GGMS01000737    MBY69940.1    ABLF02012739    ABLF02012741    GGMR01018716    MBY31335.1   

Proteomes

UP000218220    UP000235965    UP000007819    UP000036403   

PRIDE

Q04135    P21328    P21329    Q95SX7   

Pfam

PF14529   Exo_endo_phos_2        + More
PF00078   RVT_1
PF07530   PRE_C2HC
PF03372   Exo_endo_phos
PF04937   DUF659

Interpro

IPR000477   RT_dom        + More
IPR036691   Endo/exonu/phosph_ase_sf       
IPR005135   Endo/exonuclease/phosphatase       
IPR006579   Pre_C2HC_dom       
IPR037220   BED_dom       
IPR012337   RNaseH-like_sf       
IPR007021   DUF659       

SUPFAM

SSF56219   SSF56219        + More
SSF140996   SSF140996       
SSF53098   SSF53098       

Gene 3D

3.60.10.10    

ProteinModelPortal

A0A2A4IUM2   A0A2J7R579   A0A2J7R4B1   A0A2J7PVX3   A0A2J7QPI2   A0A034VFD2   + More
A0A142LX26   A0A1Y1LKN6   A0A224X6T4   Q04135   Q6R811   A0A2S2N814   A0A224XJI0   J9LYS0   A0A2S2PHL7   A0A224X746   A0A224X6U5   A0A224XIB0   A0A2H8TKI9   X1WUG2   A0A0J7K7J8   A0A2S2P698   A0A352X8G4   A0A2S2NGV8   A0A2S2NKC3   A0A224XI99   A0A023F0I3   A0A2S2P3X8   A0A2H8TF37   A0A2S2PJG2   A0A2S2P6S3   A0A2S2P6C4   A0A2S2NH02   A0A142LX53   A0A2S2QDN9   X1WIX8   A0A2S2PUS3   A0A0J7K811   J9KWJ0   A0A2S2QEE0   A0A2S2PA10   A0A0J7KC92   J9KE07   A0A2S2Q2R8   A0A2S2N9P4   J9M0H4   P21328   A0A1Y1MT28   P21329   Q95SX7   A0A2S2Q7E7   A0A2S2PBB4   A0A2S2NI07   A0A2S2PX53   J9LBF1   A0A2S2PPK8  

PDB

5G2X     E-value=0.0295283,     Score=91

KEGG

NA   

GO

GO:0003964   GO:0006313  

Length:

823

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.220080000000001

Exp number, first 60 AAs:

0.03605

Total prob of N-in:

0.00761

outside

1  -  823

 

 

Pi

344.715247

Theta

194.891553

Tajima's D

2.435769

CLR

1.118797

CSRT

0.938303084845758

Interpretation

Uncertain