Gene

KWMTBOMO15034  Validated by peptides from experiments

Annotation

gallerin_[Galleria_mellonella]

Location

Bomo_Chr25(-):3257033-3266737      View in EpiBrowser

Full name

Insecticyanin-B       + More
Insecticyanin-A      
Bilin-binding protein      
Lopap      
Apolipoprotein D      

Alternative Name

Blue biliprotein
Lipocalin-1/4
Prothrombin activator

Location in the cell

Cytoplasmic   Reliability : 1.795 Extracellular   Reliability : 1.426

CDS

ATGCTGAGGCTAGTGCTACTGACTCTGCTTGCGGCGGCGACTGCTGAAGTTATTCACGAAGGCACCTGCCCCGAGCTGAAACCGGTGAACAACTTCAACTTGACAGCTTACCAAGGCATCTGGTATGAAATCTCGAAATTTCCCAACGAATCTGAGAAGAACGGCAAATGCTCTTCAGCTGAATACAAACTGGAAGGTGACGTGGTGAAGGTCAAGAACGTGCATATCATCGACGGCGTCAAGAAGTATATAGAAGGGACGGCCAAGCTCACCGACGACGCCAATAAAGCCGCAAAGCTAACAGTCACTTTTAAGTTTGGAGAAATATCACGCGATGGATCAGTTCAAGTCTTGGCGACTGACTATAATAACTACGCCATCGCTTACAACTGCAAATACGATGACAAGAAAAAGTCTCATCAAGTGTTCGTCTGGATCCTCTCTAGAAACAAGAAGCTTGAAGGCGACGCTAAAACTGCTGTCGATAATTTCATCAAGGAACACTCCAAAGAGATAGACTCTTCGAAACTTGTGCATACCGATTTTTCAGAGGAAGCCTGCAAATTTACCAGCTCCAGCGTGATAACCGAACACGGAAAACATTAA

Protein

MLRLVLLTLLAAATAEVIHEGTCPELKPVNNFNLTAYQGIWYEISKFPNESEKNGKCSSAEYKLEGDVVKVKNVHIIDGVKKYIEGTAKLTDDANKAAKLTVTFKFGEISRDGSVQVLATDYNNYAIAYNCKYDDKKKSHQVFVWILSRNKKLEGDAKTAVDNFIKEHSKEIDSSKLVHTDFSEEACKFTSSSVITEHGKH

Description

This protein binds the blue pigments bilins.
Serine protease. Activates thrombin by cleavage of prothrombin. Does not activate factor X. When injected into rats, causes thrombus formation, fibrinogen depletion, uncoagulable blood, decreased platelet count, inhibition of collagen-induced platelet aggregation, leukocyte infiltration in lungs, congestion and hemorrhage in renal glomeruli and necrosis in renal distal tubules. Causes increase in surface expression of ICAM-1 and E-selectin on human umbilical vein endothelial cells, but does not affect expression of VCAM-1, or the expression of mediators involved in coagulation and fibrinolysis systems (TF, vWF and t-PA). Increases expression of the platelet activation inhibitors NO and PGI2. Increases viability of human umbilical vein endothelial cells, and inhibits apoptosis.

Biophysicochemical Properties

4.5 uM for Abz-YQTFFNPRTGSQ-EDDnp

Subunit

Homotetramer.
Homodimer.

Similarity

Belongs to the calycin superfamily. Lipocalin family.

Keywords

Disulfide bond   Pigment   Secreted   Signal   3D-structure   Bile pigment   Chromophore   Direct protein sequencing   Glycoprotein   Hydrolase   Protease   Prothrombin activator   Serine protease  

Feature

chain  Insecticyanin-B

Uniprot

Q95P97   Q24996   H9J5S5   A0A142D6P9   A0A2A4JUL1   I3UIH6   + More
I0C0B5   A0A2H1VQI2   A0A2W1BTZ5   Q8T118   F0UYI4   Q00629   Q00630   P00305   P09464   I4DII3   A3KEW8   A0A194RHQ1   I0C0B6   A0A3Q8TBI9   I4DN63   D5MRL5   Q5ECE3   B1B1V3   A0A194PFU7   I4DIB6   A0A194RM80   I4DLT4   I4DQS8   A0A194RGZ2   I0C0B7   D5MRK9   Q8T119   I4DN64   A0A194RI26   A0A212F388   B2NIZ3   A0A194PM32   A0A194PHH8   A0A212EXC5   A0A212EWQ0   A0A212ESV2   A0A3N0Z9L5   A0A1W5A6C6   C3Y145   A0A3N0Z9L3   A0A3Q0QX35   A0A3P9K5L3   A0A3P9K5M3   H2MUK0   H2MUJ9   E7F2R8   W5MJK5   A0A3B3DF18   A0A3B3Q487   A0A3Q0R232   A0A0N1I7I6   A0A3B1JME6   A0A3B1J8Z8   A0A3B1IW18   A0A194PFV2   A0A3P9IV14   A0A3P8UCY4   A0A3P8UJ26   A0A3P9H6J3   A0A3P8WPW0   W5KLE7   A0A3Q2D0T8   A0A3B4A9I1   A0A3P8WXM8   A0A3Q0RKG1   A0A3B3Q6Z3   A0A1N7TLC2   H2SBL0   A0A3Q0RF77   A0A3Q1GA32   A0A3Q2VVE5   B5XEY8   A0A3P8PEY1   A0A2J8WIB1   A0A3P8W739   A0A1S3QAT7   A0A2J8WID0   A0A3Q2P803   A0A3P8P7R6   A0A3P9CTM2   A0A3P9DGN0   Q4SK77   A0A3B4EUH2   A0A3B4EWA2   A0A212ETG8   F1QDM9   H3CJ67   Q16VH7  

EC Number

3.4.21.-

Pubmed

19121390    28756777    21265041    1572353    6386809    3608987    + More
4096898    8112337    3202956    3430616    22651552    26354079    16023793    16734589    11395128    18342903    11910193    11395129    15963468    18712529    18292334    22118469    10930586    18563158    17554307    23594743    29451363    29240929    25329095    24487278    25463417    21551351    20433749    25186727    15496914    17510324   

EMBL

AB050439    BAB47155.1    L41641    AAA85089.1    BABH01025081    BABH01025082    + More
KU865512    AMQ22734.1    NWSH01000611    PCG75314.1    JQ731645    AFK64814.1    JQ913012    AFH68244.1    ODYU01003843    SOQ43105.1    KZ149918    PZC77811.1    AB080223    BAB85482.1    FJ882066    ACZ68115.1    X64714    CAA45969.1    X64715    X76568    AK401101    KQ459604    BAM17723.1    KPI92171.1    AB294515    BAF47403.1    KQ460205    KPJ17092.1    JQ913013    AFH68245.1    MG897155    AZL30770.1    AK402731    BAM19353.1    AB525747    BAJ07597.1    AY829809    AY829833    AY908986    AAV91423.1    AAV91447.1    AAW88441.1    AB264632    BAG12758.1    KPI92167.1    AK401034    BAM17656.1    KPI92166.1    KPJ17091.1    AK402252    BAM18874.1    AK404774    BAM20268.1    KPJ17088.1    JQ913014    AFH68246.1    AB525741    BAJ07591.1    AB069697    BAB84676.1    AK402732    BAM19354.1    KPJ17089.1    AGBW02010599    OWR48205.1    AB436410    BAG32140.1    KPI92170.1    KPI92169.1    AGBW02011784    OWR46150.1    AGBW02011917    OWR45930.1    AGBW02012705    OWR44575.1    RJVU01001704    ROL55129.1    GG666480    EEN65585.1    ROL55130.1    BX649372    AHAT01015256    KQ460594    KPJ13950.1    KPI92172.1    KX186723    ANU05020.1    BT049607    ACI69408.1    NDHI03003390    PNJ69520.1    PNJ69522.1    CAAE01014568    CAF98955.1    AGBW02012595    OWR44741.1    CR450695    CH477592    EAT38559.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000053240    UP000007151    UP000192224    + More
UP000001554    UP000261340    UP000265180    UP000001038    UP000000437    UP000018468    UP000261560    UP000261540    UP000018467    UP000265200    UP000265120    UP000265020    UP000261520    UP000005226    UP000257200    UP000264840    UP000087266    UP000265100    UP000265000    UP000265160    UP000261460    UP000007303    UP000008820   

PRIDE

Q95P97    H9J5S5    P09464   

Pfam

PF00061   Lipocalin        + More
PF08212   Lipocalin_2
PF03973   Triabin

Interpro

IPR003057   Invtbrt_color        + More
IPR012674   Calycin       
IPR022271   Lipocalin_ApoD       
IPR022272   Lipocalin_CS       
IPR000566   Lipocln_cytosolic_FA-bd_dom       
IPR002969   ApolipopD       
IPR026222   ApoD_vertbrte       
IPR005657   Triabi/Procalin       

SUPFAM

SSF50814   SSF50814       

Gene 3D

2.40.128.20    

ProteinModelPortal

Q95P97   Q24996   H9J5S5   A0A142D6P9   A0A2A4JUL1   I3UIH6   + More
I0C0B5   A0A2H1VQI2   A0A2W1BTZ5   Q8T118   F0UYI4   Q00629   Q00630   P00305   P09464   I4DII3   A3KEW8   A0A194RHQ1   I0C0B6   A0A3Q8TBI9   I4DN63   D5MRL5   Q5ECE3   B1B1V3   A0A194PFU7   I4DIB6   A0A194RM80   I4DLT4   I4DQS8   A0A194RGZ2   I0C0B7   D5MRK9   Q8T119   I4DN64   A0A194RI26   A0A212F388   B2NIZ3   A0A194PM32   A0A194PHH8   A0A212EXC5   A0A212EWQ0   A0A212ESV2   A0A3N0Z9L5   A0A1W5A6C6   C3Y145   A0A3N0Z9L3   A0A3Q0QX35   A0A3P9K5L3   A0A3P9K5M3   H2MUK0   H2MUJ9   E7F2R8   W5MJK5   A0A3B3DF18   A0A3B3Q487   A0A3Q0R232   A0A0N1I7I6   A0A3B1JME6   A0A3B1J8Z8   A0A3B1IW18   A0A194PFV2   A0A3P9IV14   A0A3P8UCY4   A0A3P8UJ26   A0A3P9H6J3   A0A3P8WPW0   W5KLE7   A0A3Q2D0T8   A0A3B4A9I1   A0A3P8WXM8   A0A3Q0RKG1   A0A3B3Q6Z3   A0A1N7TLC2   H2SBL0   A0A3Q0RF77   A0A3Q1GA32   A0A3Q2VVE5   B5XEY8   A0A3P8PEY1   A0A2J8WIB1   A0A3P8W739   A0A1S3QAT7   A0A2J8WID0   A0A3Q2P803   A0A3P8P7R6   A0A3P9CTM2   A0A3P9DGN0   Q4SK77   A0A3B4EUH2   A0A3B4EWA2   A0A212ETG8   F1QDM9   H3CJ67   Q16VH7  

PDB

1Z24     E-value=8.0909e-43,     Score=433

KEGG

692416    K03098 apolipoprotein D and lipocalin family protein | (RefSeq) Bombyrin

GO

GO:0031409   GO:0005576   GO:0018298   GO:0016504   GO:0008236   GO:0006869   GO:0008289   GO:0007568   GO:0005737   GO:0000302   GO:0006629   GO:0030682   GO:0006508   GO:0008270   GO:0004180   GO:0005515   GO:0003676   GO:0007154   GO:0016020   GO:0005112   GO:0015930  

Subcellular location

Secreted  

SignalP

Position:   1 - 15,         Likelihood:  0.998712

 

 

Length:

201

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00949000000000001

Exp number, first 60 AAs:

0.00896

Total prob of N-in:

0.00788

outside

1  -  201

 

 

Pi

247.331717

Theta

169.235625

Tajima's D

1.284531

CLR

0.13333

CSRT

0.737013149342533

Interpretation

Uncertain