Gene

KWMTBOMO15033  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004805

Annotation

PREDICTED:_insecticyanin-A-like_[Papilio_polytes]

Location

Bomo_Chr25(+):3250168-3254513      View in EpiBrowser

Full name

Lopap       + More
Insecticyanin-B      
Insecticyanin-A      

Alternative Name

Lipocalin-1/4
Prothrombin activator
Blue biliprotein

Location in the cell

Extracellular   Reliability : 2.883

CDS

ATGTTATTGTTTCTGCTCTGTATCTCTTTGTATGGCGCTTCAGGAGCGGAGTTCACGCTACCGGGTGAGTGTCCAAATATGAAGCTGCAAGAGAACTTGGATTATACTAAGTTTTCAGGGGTATGGTACAACGTTGCAAGCTATGCAAGCGATGGACGGCCAATCTACGATTGTGCCACCCTGGACTTCCAGGAAGACAACCTTGGCTACACCCTACGGGAGACGTACGTGAATGTCGACGGGGGTAATAGAACCCAGAAGTCTTACCTGGCAAGAGTCGACCCCACGTTTGATGCTGGAAACAAGGCTCAGTTCATTGTCAGCCACGAGGGCGGTGATAAAGTTCTGCAGTTTCCGTTTTTCATCTTGTCCACTGATTACGATGAATACGCCATAGCGTACACGTGTAAAACGTTGAAGAAGAAAACCAGGACTCATTATGTTTTCACATGGATCCTCACGAGAACCAAGAACAAACTGCAAGGCGATACTCAAAAGAAAGTAGAGACTGCTCTTTCGAATTACCTAGAACTGGCCGAGCACCGAAAGCAGTTCGTATTTAAAGATTTTTCCGAAGAAGACTGCTCCTACACGGATCAATTCAAAACCGATTTCTTCACAAGCAACTTCTGGTGA

Protein

MLLFLLCISLYGASGAEFTLPGECPNMKLQENLDYTKFSGVWYNVASYASDGRPIYDCATLDFQEDNLGYTLRETYVNVDGGNRTQKSYLARVDPTFDAGNKAQFIVSHEGGDKVLQFPFFILSTDYDEYAIAYTCKTLKKKTRTHYVFTWILTRTKNKLQGDTQKKVETALSNYLELAEHRKQFVFKDFSEEDCSYTDQFKTDFFTSNFW

Description

Serine protease. Activates thrombin by cleavage of prothrombin. Does not activate factor X. When injected into rats, causes thrombus formation, fibrinogen depletion, uncoagulable blood, decreased platelet count, inhibition of collagen-induced platelet aggregation, leukocyte infiltration in lungs, congestion and hemorrhage in renal glomeruli and necrosis in renal distal tubules. Causes increase in surface expression of ICAM-1 and E-selectin on human umbilical vein endothelial cells, but does not affect expression of VCAM-1, or the expression of mediators involved in coagulation and fibrinolysis systems (TF, vWF and t-PA). Increases expression of the platelet activation inhibitors NO and PGI2. Increases viability of human umbilical vein endothelial cells, and inhibits apoptosis.

Biophysicochemical Properties

4.5 uM for Abz-YQTFFNPRTGSQ-EDDnp

Subunit

Homotetramer.

Similarity

Belongs to the calycin superfamily. Lipocalin family.

Keywords

Direct protein sequencing   Disulfide bond   Glycoprotein   Hydrolase   Protease   Prothrombin activator   Secreted   Serine protease   Signal   Pigment   3D-structure   Bile pigment   Chromophore  

Feature

chain  Lopap

Uniprot

A0A194RHP6   A0A194PM29   A0A1E1WGA5   A0A159YQU9   A0A212FJU3   A0A212EIV4   + More
A0A2W1BYB5   A0A2H1VSA5   A0A2A4JTE9   A0A0L7KXZ8   A0A2H1VQI2   A0A194RHQ1   Q24996   Q5ECE3   Q95P97   A0A2A4JUL1   A0A142D6P9   I0C0B5   I3UIH6   Q8T119   I4DII3   A0A194RM77   A0A2W1BRV6   Q00630   I0C0B6   A0A3Q8TBI9   Q8T118   A0A2A4JU62   P00305   A0A2H1VQH8   Q00629   A3KEW8   H9J5S5   I0C0B7   H9J5S6   D5MRL5   S4PVS5   D5MRK9   B2NIZ3   A0A212EIU3   B1B1V3   A0A212ETG8   A0A1A8VAG0   K1PRB6   A0A194PFU7   A0A1B6JTI0   A0A194RI26   A0A2W1BTZ5   I4DLT4   A0A2W1BRU9   A0A2R4K2Q2   I4DN63   A0A212F388   G3PXV7   A0A194RGZ2   A0A2U9BID1   A0A2A4JTG0   A0A2A4JTA8   A0A1D5B369   A0A1D5B367   A0A147AQ88   A0A2H1VQP6   B4GQ24   A0A2H1VRJ6   A0A194PFV2   I4DIB6   A0A1I9WL92   A0A2H1W3W0   B4LT40   A0A194PM32   A0A212ESV2   A0A226ETN7   A0A0N1I7I6   A0A0M4DZE9   I4DQS8   E9FY92   A0A3Q2P803   H9J5L4   A0A1W4VIZ5   A0A3B0JWS6   A0A2A4JTG4   A0A2A4JTJ4   A0A194RM80   A0A2A4JUF4   K7G6U9   A0A2A4JV91   A0A2A4JVA8   A0A034WMW6   A0A2A4JTH0   A0A2A4JV19   A0A2A4JUN1   A0A194PGK5   A0A3P9K5L3   A0A1B0FY26   B5DJ00  

EC Number

3.4.21.-

Pubmed

26354079    22118469    28756777    26227816    16023793    16734589    + More
11395128    18342903    11910193    11395129    15963468    22651552    1572353    6386809    3608987    4096898    19121390    23622113    10930586    18712529    18292334    22992520    27688756    17994087    27538518    21292972    17381049    25348373    17554307    15632085   

EMBL

KQ460205    KPJ17087.1    KQ459604    KPI92165.1    GDQN01005045    GDQN01003677    + More
JAT86009.1    JAT87377.1    KU096845    AMY63178.1    AGBW02008230    OWR53979.1    AGBW02014563    OWR41414.1    KZ149918    PZC77810.1    ODYU01003843    SOQ43104.1    NWSH01000611    PCG75315.1    JTDY01004480    KOB68097.1    SOQ43105.1    KPJ17092.1    L41641    AAA85089.1    AY829809    AY829833    AY908986    AAV91423.1    AAV91447.1    AAW88441.1    AB050439    BAB47155.1    PCG75314.1    KU865512    AMQ22734.1    JQ913012    AFH68244.1    JQ731645    AFK64814.1    AB069697    BAB84676.1    AK401101    BAM17723.1    KPI92171.1    KPJ17086.1    PZC77809.1    X64715    JQ913013    AFH68245.1    MG897155    AZL30770.1    AB080223    BAB85482.1    PCG75316.1    SOQ43103.1    X64714    CAA45969.1    AB294515    BAF47403.1    BABH01025081    BABH01025082    JQ913014    AFH68246.1    BABH01025083    BABH01025084    AB525747    BAJ07597.1    GAIX01008028    JAA84532.1    AB525741    BAJ07591.1    AB436410    BAG32140.1    OWR41413.1    AB264632    BAG12758.1    AGBW02012595    OWR44741.1    HAEJ01016135    SBS56592.1    JH816675    EKC24158.1    KPI92167.1    GECU01005172    JAT02535.1    KPJ17089.1    PZC77811.1    AK402252    BAM18874.1    PZC77812.1    MF138149    AVV48151.1    AK402731    BAM19353.1    AGBW02010599    OWR48205.1    KPJ17088.1    CP026249    AWP03818.1    PCG75325.1    NWSH01000617    PCG75275.1    PCG75276.1    GCES01005579    JAR80744.1    SOQ43106.1    CH479187    EDW39696.1    SOQ43102.1    KPI92172.1    AK401034    BAM17656.1    KPI92166.1    KU932276    APA33912.1    ODYU01006132    SOQ47717.1    CH940649    EDW63871.2    KPI92170.1    AGBW02012705    OWR44575.1    LNIX01000002    OXA60985.1    KQ460594    KPJ13950.1    CP012523    ALC37954.1    AK404774    BAM20268.1    GL732527    EFX87818.1    OUUW01000004    SPP79950.1    PCG75327.1    PCG75331.1    KPJ17091.1    PCG75328.1    AGCU01131987    AGCU01131988    PCG75330.1    PCG75332.1    GAKP01003839    JAC55113.1    PCG75335.1    PCG75333.1    PCG75334.1    KPI92163.1    AJVK01031154    CH379060    EDY70295.2   

Proteomes

UP000053240    UP000053268    UP000007151    UP000218220    UP000037510    UP000005204    + More
UP000005408    UP000007635    UP000246464    UP000008744    UP000008792    UP000198287    UP000092553    UP000000305    UP000265000    UP000192221    UP000268350    UP000007267    UP000265180    UP000092462    UP000001819   

PRIDE

Q95P97    H9J5S5    H9J5S6    H9J5L4   

Pfam

PF00061   Lipocalin        + More
PF03973   Triabin
PF08212   Lipocalin_2

Interpro

IPR000566   Lipocln_cytosolic_FA-bd_dom        + More
IPR022272   Lipocalin_CS       
IPR003057   Invtbrt_color       
IPR012674   Calycin       
IPR022271   Lipocalin_ApoD       
IPR002449   Retinol-bd/Purpurin       
IPR005657   Triabi/Procalin       
IPR002969   ApolipopD       
IPR026222   ApoD_vertbrte       

SUPFAM

SSF50814   SSF50814       

Gene 3D

2.40.128.20    

ProteinModelPortal

A0A194RHP6   A0A194PM29   A0A1E1WGA5   A0A159YQU9   A0A212FJU3   A0A212EIV4   + More
A0A2W1BYB5   A0A2H1VSA5   A0A2A4JTE9   A0A0L7KXZ8   A0A2H1VQI2   A0A194RHQ1   Q24996   Q5ECE3   Q95P97   A0A2A4JUL1   A0A142D6P9   I0C0B5   I3UIH6   Q8T119   I4DII3   A0A194RM77   A0A2W1BRV6   Q00630   I0C0B6   A0A3Q8TBI9   Q8T118   A0A2A4JU62   P00305   A0A2H1VQH8   Q00629   A3KEW8   H9J5S5   I0C0B7   H9J5S6   D5MRL5   S4PVS5   D5MRK9   B2NIZ3   A0A212EIU3   B1B1V3   A0A212ETG8   A0A1A8VAG0   K1PRB6   A0A194PFU7   A0A1B6JTI0   A0A194RI26   A0A2W1BTZ5   I4DLT4   A0A2W1BRU9   A0A2R4K2Q2   I4DN63   A0A212F388   G3PXV7   A0A194RGZ2   A0A2U9BID1   A0A2A4JTG0   A0A2A4JTA8   A0A1D5B369   A0A1D5B367   A0A147AQ88   A0A2H1VQP6   B4GQ24   A0A2H1VRJ6   A0A194PFV2   I4DIB6   A0A1I9WL92   A0A2H1W3W0   B4LT40   A0A194PM32   A0A212ESV2   A0A226ETN7   A0A0N1I7I6   A0A0M4DZE9   I4DQS8   E9FY92   A0A3Q2P803   H9J5L4   A0A1W4VIZ5   A0A3B0JWS6   A0A2A4JTG4   A0A2A4JTJ4   A0A194RM80   A0A2A4JUF4   K7G6U9   A0A2A4JV91   A0A2A4JVA8   A0A034WMW6   A0A2A4JTH0   A0A2A4JV19   A0A2A4JUN1   A0A194PGK5   A0A3P9K5L3   A0A1B0FY26   B5DJ00  

PDB

1Z24     E-value=6.49628e-15,     Score=193

KEGG

101745175    K03098 apolipoprotein D and lipocalin family protein | (RefSeq) lopap-like

GO

GO:0031409   GO:0034632   GO:0005501   GO:0016504   GO:0008236   GO:0005576   GO:0018298   GO:0030682   GO:0006869   GO:0008289   GO:0007568   GO:0016021   GO:0033500   GO:0070328   GO:0006979   GO:0005615   GO:0008340   GO:0005737   GO:0006629   GO:0000302   GO:2000098   GO:0071638   GO:2000405   GO:0060588   GO:0010642   GO:0048471   GO:1900016   GO:0042308   GO:0051895   GO:0048662   GO:0006006   GO:0015485   GO:0006508   GO:0008270   GO:0004180   GO:0005515   GO:0003676   GO:0007154   GO:0016020   GO:0005112   GO:0015930  

Subcellular location

Secreted  

SignalP

Position:   1 - 15,         Likelihood:  0.997945

 

 

Length:

211

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.17613

Exp number, first 60 AAs:

0.1703

Total prob of N-in:

0.01198

outside

1  -  211

 

 

Pi

180.752653

Theta

153.067253

Tajima's D

0.694123

CLR

0.011748

CSRT

0.572371381430928

Interpretation

Uncertain