Gene

KWMTBOMO14994  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA004876

Annotation

hypothetical_protein_KGM_22030_[Danaus_plexippus]

Location

Bomo_Chr25(-):1968412-1985096      View in EpiBrowser

Full name

Molybdopterin synthase catalytic subunit       + More
Multifunctional fusion protein      

Alternative Name

Molybdenum cofactor synthesis protein 2 large subunit
Molybdenum cofactor synthesis protein 2B

Location in the cell

Cytoplasmic   Reliability : 1.96 Nuclear   Reliability : 2.053

CDS

ATGGACCATATAAAATTAACAGTAGATAGACTGTCAGTCGAAGAAATAAGTGAACTTGTAACTGATGATAGCTGCGGTGCAATTTCATTGTTTATTGGCACAACTAGAGACAATTTTGAAGGAAAAAAGGTACTAAGGCTGGAATATGAGGCCTACGAATCTATGGCCATAAAAGCGATAAAAACAATTTGTGATGACGTCAGACAGAAATGGCCGCTTGTACACGGAATAGCTATTTATCACAGGTTGGGCAACGTTCCGTGTCGAGAGGCGTCTGTCGTGATAGCCATAAGCAGTCCGCAGAGGAAGGACTCTTTAAGCGCCGTATCCTACACCATCGATCAGCTAAAGGCCTCAGTTCCTATATGGAAGAAGGAAATATACGACGGACAACCCGCCCAATGGAAGGAGAATCGTGAATTTCCGTCGAAATCGGAGTCGAAGAACATCGACAAAAATTTAGTACAGATCAACGTTTCGAGCGAAGAACTACAGAAGCGGATACAGAACTTTATTGAGAGGAAACGGGAGCAGGTTAATTACGGCAACATACAGGACTTCGTGCCGGGGCGGCGTCAGGACGAGGAGGACACGTGCGCGCGGGTCAGGGCGCAGTTCGTCAAACGAAACGATTCTAAAGGACATTTGAAAGTATACAAAGTCAACAACGAATGGGGTCCACAAACGGTTAGTTTCAGTGGGGTCCCCGTTGGGTCCTCGGACCCGGGCCTGCCGCCCTCCGTAGCCGAGCGAGTCGTGGCCGTCGAGAAGTTCCTTAACCTGGGCCCCGTCTCCAAGGACATCTTCAAGCGGCTCAAGGACATGGAGGACAGGATCGCGTATCTGCAGTCGGTGTCGCCGGAATACGCGCAGTTCTGGAAAACAAAGGCAGACCTGGAAGAGATAAAATCTGAGGATCGTATAGATTACGCGTTCTCTGCTGAAGACATCGCGAGGAAGATCGACCAATTGGAAAAGGCCAATTTATGA

Protein

MDHIKLTVDRLSVEEISELVTDDSCGAISLFIGTTRDNFEGKKVLRLEYEAYESMAIKAIKTICDDVRQKWPLVHGIAIYHRLGNVPCREASVVIAISSPQRKDSLSAVSYTIDQLKASVPIWKKEIYDGQPAQWKENREFPSKSESKNIDKNLVQINVSSEELQKRIQNFIERKREQVNYGNIQDFVPGRRQDEEDTCARVRAQFVKRNDSKGHLKVYKVNNEWGPQTVSFSGVPVGSSDPGLPPSVAERVVAVEKFLNLGPVSKDIFKRLKDMEDRIAYLQSVSPEYAQFWKTKADLEEIKSEDRIDYAFSAEDIARKIDQLEKANL

Description

Catalytic subunit of the molybdopterin synthase complex, a complex that catalyzes the conversion of precursor Z into molybdopterin. Acts by mediating the incorporation of 2 sulfur atoms from thiocarboxylated MOCS2A into precursor Z to generate a dithiolene group.
Acts as a sulfur carrier required for molybdopterin biosynthesis. Component of the molybdopterin synthase complex that catalyzes the conversion of precursor Z into molybdopterin by mediating the incorporation of 2 sulfur atoms into precursor Z to generate a dithiolene group. In the complex, serves as sulfur donor by being thiocarboxylated (-COSH) at its C-terminus by MOCS3. After interaction with MOCS2B, the sulfur is then transferred to precursor Z to form molybdopterin.

Catalytic Activity

2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-NH-CH2-C(O)SH + cyclic pyranopterin phosphate + H2O = 2 [molybdopterin-synthase sulfur-carrier protein]-C-terminal Gly-Gly + 4 H(+) + molybdopterin

Subunit

Heterotetramer; composed of 2 small (MOCS2A) and 2 large (MOCS2B) subunits.

Miscellaneous

This protein is produced by a bicistronic gene which also produces the large subunit (MOCS2A).
This protein is produced by a bicistronic gene which also produces the large subunit (MOCS2B).

Similarity

Belongs to the MoaE family. MOCS2B subfamily.
Belongs to the MoaD family. MOCS2A subfamily.

Keywords

Complete proteome   Cytoplasm   Molybdenum cofactor biosynthesis   Reference proteome   Transferase  

Feature

chain  Molybdopterin synthase catalytic subunit

Uniprot

H9J5T6   A0A2H1WSI8   A0A3S2P0I8   A0A2W1BK53   A0A2A4JUN4   A0A212EUY9   + More
A0A2A4JSY2   A0A194RHC9   A0A194PLU0   A0A0L7KYA3   A0A158NAQ4   A0A0M8ZWH9   A0A1Y1NBL5   A0A195EKF0   A0A088AB86   A0A2J7R257   A0A2J7R254   A0A2A3EQ75   A0A0L7QTK5   A0A067RBT2   A0A195CWA1   E2BHL1   A0A026X236   A0A1B0CXZ7   A0A151WWL1   B3M268   D6WT96   A0A1A9XNR3   Q9VBX2   B4PUD1   A0A1W4VM16   A0A1B0GDX2   B4NJW0   A0A1B0AHD5   B4QUC0   A0A1A9W2Q8   B4IJG7   B3P6R5   A0A0P6GA46   N6TIW5   A0A1W4V8X1   B4JHP4   A0A1Y1NF28   A0A1B0BB51   A0A0P4XBZ7   Q297G3   B4GE20   A0A3B0JQJ4   A0A195FRI6   A0A0P5EB01   B4KBH3   A0A0Q9WL45   B4LVP8   A0A0R3NNF3   A0A0P4VT82   A0A0K8U900   A0A0K8WI54   A0A0K8W6G8   A0A1I8MV61   C1C0B0   A0A1I8PJL7   C1BPI4  

EC Number

2.8.1.12

Pubmed

19121390    28756777    22118469    26354079    26227816    21347285    + More
28004739    24845553    20798317    24508170    30249741    17994087    18362917    19820115    10731132    12537572    12537569    18237443    23537049    15632085    25315136   

EMBL

BABH01025217    BABH01025218    BABH01025219    BABH01025220    BABH01025221    ODYU01010736    + More
SOQ56035.1    RSAL01000009    RVE53864.1    KZ150226    PZC72053.1    NWSH01000636    PCG75120.1    AGBW02012265    OWR45318.1    PCG75121.1    KQ460205    KPJ16982.1    KQ459604    KPI92070.1    JTDY01004414    KOB68192.1    ADTU01009937    KQ435848    KOX71043.1    GEZM01009852    JAV94260.1    KQ978739    KYN28718.1    NEVH01008200    PNF34911.1    PNF34914.1    KZ288194    PBC33840.1    KQ414741    KOC61973.1    KK852805    KDR16124.1    KQ977276    KYN04434.1    GL448301    EFN84820.1    KK107054    QOIP01000004    EZA61459.1    RLU23575.1    AJWK01035129    KQ982686    KYQ52292.1    CH902617    KQ971352    EFA06321.2    AE014297    AY047520    CM000160    CCAG010012069    CH964272    CM000364    CH480848    CH954182    GDIQ01257722    GDIP01046269    GDIQ01036440    JAM57446.1    JAN58297.1    APGK01036258    KB740937    ENN77778.1    CH916369    GEZM01009854    JAV94257.1    JXJN01011305    GDIP01244861    JAI78540.1    CM000070    CH479182    OUUW01000007    SPP83233.1    KQ981305    KYN42897.1    GDIP01144658    JAJ78744.1    CH933806    CH940650    KRF82695.1    KRT00592.1    GDRN01099152    JAI58823.1    GDHF01029180    JAI23134.1    GDHF01001486    JAI50828.1    GDHF01005834    JAI46480.1    BT080289    ACO14713.1    BT076513    ACO10937.1   

Proteomes

UP000005204    UP000283053    UP000218220    UP000007151    UP000053240    UP000053268    + More
UP000037510    UP000005205    UP000053105    UP000078492    UP000005203    UP000235965    UP000242457    UP000053825    UP000027135    UP000078542    UP000008237    UP000053097    UP000279307    UP000092461    UP000075809    UP000007801    UP000007266    UP000092443    UP000000803    UP000002282    UP000192221    UP000092444    UP000007798    UP000092445    UP000000304    UP000091820    UP000001292    UP000008711    UP000019118    UP000001070    UP000092460    UP000001819    UP000008744    UP000268350    UP000078541    UP000009192    UP000008792    UP000095301    UP000095300   

PRIDE

H9J5T6    Q9VBX2    B4PUD1    B4QUC0    B3P6R5    B4GE20    + More

Pfam

PF02391   MoaE        + More
PF02597   ThiS

Interpro

IPR036563   MoaE_sf        + More
IPR003448   Mopterin_biosynth_MoaE       
IPR028888   MOCS2B_euk       
IPR003749   ThiS/MoaD-like       
IPR016155   Mopterin_synth/thiamin_S_b       
IPR028887   MOCS2A       
IPR012675   Beta-grasp_dom_sf       

SUPFAM

SSF54690   SSF54690        + More
SSF54285   SSF54285       

Gene 3D

3.90.1170.40     3.10.20.30    

CDD

cd00756  MoaE

ProteinModelPortal

H9J5T6   A0A2H1WSI8   A0A3S2P0I8   A0A2W1BK53   A0A2A4JUN4   A0A212EUY9   + More
A0A2A4JSY2   A0A194RHC9   A0A194PLU0   A0A0L7KYA3   A0A158NAQ4   A0A0M8ZWH9   A0A1Y1NBL5   A0A195EKF0   A0A088AB86   A0A2J7R257   A0A2J7R254   A0A2A3EQ75   A0A0L7QTK5   A0A067RBT2   A0A195CWA1   E2BHL1   A0A026X236   A0A1B0CXZ7   A0A151WWL1   B3M268   D6WT96   A0A1A9XNR3   Q9VBX2   B4PUD1   A0A1W4VM16   A0A1B0GDX2   B4NJW0   A0A1B0AHD5   B4QUC0   A0A1A9W2Q8   B4IJG7   B3P6R5   A0A0P6GA46   N6TIW5   A0A1W4V8X1   B4JHP4   A0A1Y1NF28   A0A1B0BB51   A0A0P4XBZ7   Q297G3   B4GE20   A0A3B0JQJ4   A0A195FRI6   A0A0P5EB01   B4KBH3   A0A0Q9WL45   B4LVP8   A0A0R3NNF3   A0A0P4VT82   A0A0K8U900   A0A0K8WI54   A0A0K8W6G8   A0A1I8MV61   C1C0B0   A0A1I8PJL7   C1BPI4  

PDB

5MPO     E-value=3.12718e-40,     Score=414

KEGG

101736222    K03635 molybdopterin synthase catalytic subunit [EC:2.8.1.12] | (RefSeq) molybdopterin synthase cata

PATHWAY

00790    Folate biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
04122    Sulfur relay system - Bombyx mori (domestic silkworm)

GO

GO:0006777   GO:0005829   GO:0030366   GO:0019008   GO:0005737   GO:0000166   GO:0005700   GO:0006338   GO:0005671   GO:0032324   GO:0004402   GO:0016573   GO:0006419   GO:0003676   GO:0007154   GO:0016020   GO:0005112   GO:0015930  

Subcellular location

Cytoplasm  

Length:

329

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.000770000000000001

Exp number, first 60 AAs:

0.00041

Total prob of N-in:

0.04415

outside

1  -  329

 

 

Pi

215.101656

Theta

167.659235

Tajima's D

0.82358

CLR

0.746912

CSRT

0.616169191540423

Interpretation

Uncertain