Gene

KWMTBOMO14991

Pre Gene Modal

BGIBMGA004877

Annotation

PREDICTED:_alanine--tRNA_ligase?_mitochondrial_[Amyelois_transitella]

Location

Bomo_Chr25(-):1937378-1949886      View in EpiBrowser

Full name

Alanine--tRNA ligase, mitochondrial      

Alternative Name

Alanyl-tRNA synthetase

Location in the cell

Cytoplasmic   Reliability : 2.005

CDS

ATGTTACGTGTACCGGTTAGAAAAAGCACAGGTTTCACACGAATGAAGTCTACCAGTAGTATTCGAAATACGTTTATAAAATATTTTATCGATAATCATGCCCATAAATTCGTGAAATCAAGTTCAGTTGTACCGTTATGTGACCCGACAGTTCCATTTGTAAATGCGGGAATGAACCAGTTCAAAGGTGTATTCCTTGGCACAGTTAATGCACCATGTCCGAGAGCTGTAAATTCACAGAAGTGCATAAGGGTTGGGGGCAAGCACAATGACTTGGATTTAGTTGGAACTGATAGTCATCATCACACATTCTTTGAGATGTTGGGCAATTGGTCCTTTGGTGACTACTATAAGAAAGAGGCATGTCAAATGGCATGGGATCTGCTGCTCGGTCCCTATCGCTTGAAGCCTGATGATTTGGTTGTCACATACTTTGCCGGTGATCCTACCATGAAACTGAATGAGGATAGAGAATGCAGAGATATATGGAAGTCCCTTGGAGTGTCTCCGAGTCGTATAAAAGGTCTTGGAGCTTCAGATAATTTCTGGGAGATGGGATTGACTGGGCCTTGCGGTCCATGTACTGAAATACATGTTGTCAACCCTGACGGAAGCCTGACCGAAATCTGGAATTTGGTGTTTATACAGTTTAATAGGGAAACAGATGGTTCAGTTACCTCTCTGTCTCGTCATCACGTGGATACTGGCATGGGATTAGAAAGAATGGCGAAGTTGCTGCAAGGCGTACCCTCTAATTACGACACAGATATTTTCAAACCTCTCATCAAGGCGATCGAGAAGAACAGCAAAAATGTACCAGCTTATAGTGGTCAGTACACGGAGAGTTCAACATTGGACTGTGCTTACAGGCGGCTCGCTGACCACTCGAGGATGATCAGTGTCTGTTTAGCTGACGGTGTCTTCCCTGCCAACAGCTTAAATGTAAAGCAAATAATGCGTAAATCGTTCAAAATATGCTCTGATGTCTTCAACAACCCTCACCTGCTGTCGAATTTATATGATGAAGTTGCAGCGACCTTGGGCTCTACCTATCCAGAGCTAGTGAGCAACAAGTCGAGTGCGAAACTTATTATTGAGCACGAAGCCCAGGCCTATGCGAAGATGAGAGCTGGTCTGGTGAAGAAGTGGAAGGACCTGGTGATGAGGTACCCCGAGGTTGAAAGCCTCAATGATGTCGAGACACCTGGATTCGCTTTAGGATATAAGGAGTTCAAAGAGACTGTTTCAAAACTTAACACATCTATAATACCCGGAGAATTGATATTCAAACTTTACGATACTCACGGCTTGCAAGAGGAACTAATAAATAGAATAGCAGAATTAAACAACATGACCACAGACAAGGAGGGCTTCCAACAACTTCTGTCAAAGCACAAACACAGACACAAGACTGCATTCAAAGAGCAAGCTTTAAACAAAACCATATTATTCAATGATGCAATCGATCGATTAATTAAAAACGGAATTAGTAGAACCAATGATCTTTTTAAATATAATTACGAGATCACAGACAGTAAACTTGAATTTAAAACTTTGACAACGAAATTATGTGCCATTTTGAATGAGGACTGTCAATGGATTGACGTTTTAGATACAATCGAAAATAGACCTTATTATTTGGTAACAGAAGACACGAATTTCTATTGCGAGGAGGGTGGTCAGATATCAGACTCTGGTGTTGCAAGAATCGACGAACATTTATCATTTAAAGTCGATAGTGTTTTTAAAATTCGAGATTTTGTCTTCCACAAAGGCTGTTTTCTAGTTGATAAAAATTATAGTGCTTGTGTTAAGTGCGGTAGTGACGTAACTTTAATTATAGATGGTGAGAAAAGACTTAGGGTCATACAAAATCACACGGCCATCCATCTTTTGAATGCAGCTATCAGGAAAGCATTACCAAACAGTGTTGTAAGTCAAATTGGTTCTAAAGTGACTGACAGGGGCTTGTATCTGAATCTGTCAGTTTATGGTGAGAAATTATCAGAAGATGTCCTATTGGCCGCTGAATGTATGATCAGGGAATCCATAGAATCGAATGCTCGAATAGAAACGAAAATATTAGACAGCGTCCAACTGGCTCTCGAAGACTCAGTGGTCACCGTTCCGGGAGAGACTTACCCGGAGACCGGACTTAGATTGGTGATAATACAGGAGCCTCTGATCTCCAAGGAATTGTGTTGCGGTACTCACGCGCCATCTGCCGGCGCGCTGCGGGACGTGTGCATCACGCAGGCCAAGGGGCTCGGGACCAACGCCCCCACCGTGTACTGCGTGTCCGGGGAGGCGGCCCGGCAGGCAAGGGAGCTGTTCTGCCGTACAAAAAAATTGGAGGAAGTGGTTGATCTGATGGACCCTGAGCGACTTAGAGAGGAGGTGGCGGACATCCGGCGCGCCCTCGGGGACGTGTGCGGGGACCGCGGCGCTCCCCGCGGCGAGCTGGCGCGCTGCCTGACCGCTCTGCGCCGCGCCGCCGCCCGCCCCGCGCCGCACCCCGAGCCGCACCCCGCGCCCGCCCTGCAGGCCATCGCTGAGGCGGAGATCCGTGAAGCGATCCAAGAGGCTGTTGATGGCGGTCGTAGGTTCGTGGTGCAGTTTGTGCGGTGCTCCTACCTCATGCAGGACTCCGGGCTGGCGTGCGCGCTGCGGTCCGGGGGCGCCCTGCCCGCCCTGCTGCTGGGCTGCGCGGGGGGCGTCATCCACGCGCAGGCGCACGTGCCCAAGGATCTAGTGACACAGTCCTTCACGGCGGAGCGCTGGTTGGGCTGCATCCTCCCCGTGTTCAGAGCCGCCGCGCAGCCCCCCGCCTGCGGAGACTCCGCCCTCACGCGAGCTGCGATGACTCCCACCAAGGTGAGCCTGATCGACTGTGAGGATCTAGTGCAGGACGCCATGCGGGCCGCCATCAGGTTCGCGCAGGCGCACGCGCACGCGCCCCGCCGCCCCGCCGACAAGAACAGGCAACACAATTAG

Protein

MLRVPVRKSTGFTRMKSTSSIRNTFIKYFIDNHAHKFVKSSSVVPLCDPTVPFVNAGMNQFKGVFLGTVNAPCPRAVNSQKCIRVGGKHNDLDLVGTDSHHHTFFEMLGNWSFGDYYKKEACQMAWDLLLGPYRLKPDDLVVTYFAGDPTMKLNEDRECRDIWKSLGVSPSRIKGLGASDNFWEMGLTGPCGPCTEIHVVNPDGSLTEIWNLVFIQFNRETDGSVTSLSRHHVDTGMGLERMAKLLQGVPSNYDTDIFKPLIKAIEKNSKNVPAYSGQYTESSTLDCAYRRLADHSRMISVCLADGVFPANSLNVKQIMRKSFKICSDVFNNPHLLSNLYDEVAATLGSTYPELVSNKSSAKLIIEHEAQAYAKMRAGLVKKWKDLVMRYPEVESLNDVETPGFALGYKEFKETVSKLNTSIIPGELIFKLYDTHGLQEELINRIAELNNMTTDKEGFQQLLSKHKHRHKTAFKEQALNKTILFNDAIDRLIKNGISRTNDLFKYNYEITDSKLEFKTLTTKLCAILNEDCQWIDVLDTIENRPYYLVTEDTNFYCEEGGQISDSGVARIDEHLSFKVDSVFKIRDFVFHKGCFLVDKNYSACVKCGSDVTLIIDGEKRLRVIQNHTAIHLLNAAIRKALPNSVVSQIGSKVTDRGLYLNLSVYGEKLSEDVLLAAECMIRESIESNARIETKILDSVQLALEDSVVTVPGETYPETGLRLVIIQEPLISKELCCGTHAPSAGALRDVCITQAKGLGTNAPTVYCVSGEAARQARELFCRTKKLEEVVDLMDPERLREEVADIRRALGDVCGDRGAPRGELARCLTALRRAAARPAPHPEPHPAPALQAIAEAEIREAIQEAVDGGRRFVVQFVRCSYLMQDSGLACALRSGGALPALLLGCAGGVIHAQAHVPKDLVTQSFTAERWLGCILPVFRAAAQPPACGDSALTRAAMTPTKVSLIDCEDLVQDAMRAAIRFAQAHAHAPRRPADKNRQHN

Description

Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction: alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged tRNA(Ala) via its editing domain.

Catalytic Activity

ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

Cofactor

Zn(2+)

Subunit

Monomer.

Similarity

Belongs to the class-II aminoacyl-tRNA synthetase family.

Keywords

Aminoacyl-tRNA synthetase   ATP-binding   Complete proteome   Ligase   Metal-binding   Mitochondrion   Nucleotide-binding   Protein biosynthesis   Reference proteome   RNA-binding   Transit peptide   tRNA-binding   Zinc  

Feature

chain  Alanine--tRNA ligase, mitochondrial

Uniprot

H9J5T7   A0A2W1BGX2   A0A2A4JTU9   A0A2H1WJU5   A0A212EV07   A0A194RHR8   + More
A0A194PFL1   A0A0K8VKZ7   A0A034VNX9   A0A1I8MSU7   A0A067QZS7   A0A1I8PLQ2   A0A139WES2   A0A0L0BR25   A0A0A1WUE8   A0A1B0A6D9   A0A1B0GAR2   A0A084WRQ3   A0A1B0GAR1   A0A182JTG4   W8BT75   A0A0J9RP52   B4HUE1   A0A2M4AHA0   Q16YK4   B3NG98   A0A182QNM4   A0A182JF17   A0A182VJY6   A0A182FGC2   B4IX05   A0A182TYX1   A0A182WWM3   B4N5H5   A0A182NJT8   B4PIR9   A0A182L3H0   A0A2M4BC40   A0A1A9X0J8   A0A1B0C7Y5   W5J6J0   Q7PF58   A0A1W4UF09   A0A1A9VIR7   A0A182P134   B4KWZ9   A0A182GF14   A0A1B0F947   B3M3R9   Q9VRJ1   A0A182I6V9   Q29FC2   A0A1Q3F379   A0A182Y314   N6UN91   B4LFQ6   U4TYE1   A0A0M4EY88   A0A1Y1N1B4   B4H1I9   A0A0C9QHG1   A0A182R9U9   B0WRV7   A0A154PPG0   A0A182WHX8   A0A3B0K3B0   A0A1J1IMB8   E2AK12   A0A182MRN7   A0A088A7M6   A0A1B0D4A3   E9ILZ9   A0A1B0CM04   A0A2A3E8H9   A0A1B6D1Y3   A0A151WX43   A0A195CGJ4   A0A0L7R3K3   A0A195F8T4   A0A195AYK1   A0A158NPC5   F4WF08   A0A026VUW1   E2B7L7   A0A0N0BG91   A0A310SR19   A0A0N8EER4   A0A0N8AW53   A0A0P5N6Y1   A0A0P5PE19   A0A0P6H880   A0A0P5NT52   A0A0P6FKB7   A0A0N8EK49   A0A0P6H7R4   E9GS46   A0A0P6GB56   A0A0P6BCV0   A0A1B6GXK8  

EC Number

6.1.1.7

Pubmed

19121390    28756777    22118469    26354079    25348373    25315136    + More
24845553    18362917    19820115    26108605    25830018    24438588    24495485    22936249    17994087    17510324    17550304    20966253    20920257    23761445    12364791    14747013    17210077    26483478    11035802    10731132    12537572    12537569    11532948    15053877    15632085    25244985    23537049    28004739    20798317    21282665    21347285    21719571    24508170    21292972   

EMBL

BABH01025223    BABH01025224    BABH01025225    KZ150226    PZC72050.1    NWSH01000636    + More
PCG75118.1    ODYU01009052    SOQ53182.1    AGBW02012265    OWR45316.1    KQ460205    KPJ16984.1    KQ459604    KPI92072.1    GDHF01032285    GDHF01012753    JAI20029.1    JAI39561.1    GAKP01014788    JAC44164.1    KK852807    KDR16062.1    KQ971354    KYB26355.1    JRES01001494    KNC22487.1    GBXI01011613    JAD02679.1    CCAG010015502    ATLV01026176    KE525407    KFB52897.1    GAMC01006422    JAC00134.1    CM002912    KMY97711.1    CH480817    EDW50562.1    GGFK01006833    MBW40154.1    CH477515    EAT39703.1    CH954178    EDV50999.1    AXCN02000522    CH916366    EDV96311.1    CH964101    EDW79614.1    CM000159    EDW93489.1    GGFJ01001452    MBW50593.1    JXJN01025925    ADMH02002070    ETN59616.1    AAAB01008807    EAA45453.4    CH933809    EDW18620.1    JXUM01059063    JXUM01059064    KQ562033    KXJ76855.1    CH902618    EDV40362.1    AF188716    AE014296    AY128439    BT001744    APCN01003702    CH379067    EAL31333.2    GFDL01013029    JAV22016.1    APGK01025214    KB740577    ENN80192.1    CH940647    EDW69283.1    KB631756    ERL85827.1    CP012525    ALC43082.1    GEZM01016656    JAV91218.1    CH479202    EDW30166.1    GBYB01013899    GBYB01014073    GBYB01014481    JAG83666.1    JAG83840.1    JAG84248.1    DS232061    EDS33522.1    KQ435012    KZC13771.1    OUUW01000012    SPP87162.1    CVRI01000055    CRL01391.1    GL440135    EFN66222.1    AXCM01000491    AJVK01024242    AJVK01024243    GL764129    EFZ18357.1    AJWK01017984    KZ288347    PBC27582.1    GEDC01017608    JAS19690.1    KQ982668    KYQ52509.1    KQ977791    KYM99837.1    KQ414663    KOC65413.1    KQ981727    KYN36791.1    KQ976703    KYM77122.1    ADTU01022284    ADTU01022285    GL888115    EGI67059.1    KK107894    EZA47311.1    GL446193    EFN88295.1    KQ435789    KOX74438.1    KQ761467    OAD57518.1    GDIQ01033956    JAN60781.1    GDIQ01237035    GDIQ01202048    JAK14690.1    GDIQ01146469    GDIQ01099779    JAL05257.1    GDIQ01196493    GDIQ01129929    GDIQ01128570    JAL21797.1    GDIQ01035455    JAN59282.1    GDIQ01138122    JAL13604.1    GDIQ01143765    GDIQ01049157    JAN45580.1    GDIQ01018876    JAN75861.1    GDIQ01033000    JAN61737.1    GL732561    EFX77730.1    GDIQ01038459    JAN56278.1    GDIP01018109    JAM85606.1    GECZ01002605    JAS67164.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053240    UP000053268    UP000095301    + More
UP000027135    UP000095300    UP000007266    UP000037069    UP000092445    UP000092444    UP000030765    UP000075881    UP000001292    UP000008820    UP000008711    UP000075886    UP000075880    UP000075903    UP000069272    UP000001070    UP000075902    UP000076407    UP000007798    UP000075884    UP000002282    UP000075882    UP000091820    UP000092460    UP000000673    UP000007062    UP000192221    UP000078200    UP000075885    UP000009192    UP000069940    UP000249989    UP000092443    UP000007801    UP000000803    UP000075840    UP000001819    UP000076408    UP000019118    UP000008792    UP000030742    UP000092553    UP000008744    UP000075900    UP000002320    UP000076502    UP000075920    UP000268350    UP000183832    UP000000311    UP000075883    UP000005203    UP000092462    UP000092461    UP000242457    UP000075809    UP000078542    UP000053825    UP000078541    UP000078540    UP000005205    UP000007755    UP000053097    UP000008237    UP000053105    UP000000305   

PRIDE

H9J5T7    Q9VRJ1   

Pfam

PF01411   tRNA-synt_2c        + More
PF07973   tRNA_SAD
PF07074   TRAP-gamma

Interpro

IPR018162   Ala-tRNA-ligase_IIc_anticod-bd        + More
IPR009000   Transl_B-barrel_sf       
IPR018163   Thr/Ala-tRNA-synth_IIc_edit       
IPR018165   Ala-tRNA-synth_IIc_core       
IPR002318   Ala-tRNA-lgiase_IIc       
IPR018164   Ala-tRNA-synth_IIc_N       
IPR012947   tRNA_SAD       
IPR023033   Ala_tRNA_ligase_euk/bac       
IPR009779   SSR3       

SUPFAM

SSF101353   SSF101353        + More
SSF50447   SSF50447       
SSF55186   SSF55186       

ProteinModelPortal

H9J5T7   A0A2W1BGX2   A0A2A4JTU9   A0A2H1WJU5   A0A212EV07   A0A194RHR8   + More
A0A194PFL1   A0A0K8VKZ7   A0A034VNX9   A0A1I8MSU7   A0A067QZS7   A0A1I8PLQ2   A0A139WES2   A0A0L0BR25   A0A0A1WUE8   A0A1B0A6D9   A0A1B0GAR2   A0A084WRQ3   A0A1B0GAR1   A0A182JTG4   W8BT75   A0A0J9RP52   B4HUE1   A0A2M4AHA0   Q16YK4   B3NG98   A0A182QNM4   A0A182JF17   A0A182VJY6   A0A182FGC2   B4IX05   A0A182TYX1   A0A182WWM3   B4N5H5   A0A182NJT8   B4PIR9   A0A182L3H0   A0A2M4BC40   A0A1A9X0J8   A0A1B0C7Y5   W5J6J0   Q7PF58   A0A1W4UF09   A0A1A9VIR7   A0A182P134   B4KWZ9   A0A182GF14   A0A1B0F947   B3M3R9   Q9VRJ1   A0A182I6V9   Q29FC2   A0A1Q3F379   A0A182Y314   N6UN91   B4LFQ6   U4TYE1   A0A0M4EY88   A0A1Y1N1B4   B4H1I9   A0A0C9QHG1   A0A182R9U9   B0WRV7   A0A154PPG0   A0A182WHX8   A0A3B0K3B0   A0A1J1IMB8   E2AK12   A0A182MRN7   A0A088A7M6   A0A1B0D4A3   E9ILZ9   A0A1B0CM04   A0A2A3E8H9   A0A1B6D1Y3   A0A151WX43   A0A195CGJ4   A0A0L7R3K3   A0A195F8T4   A0A195AYK1   A0A158NPC5   F4WF08   A0A026VUW1   E2B7L7   A0A0N0BG91   A0A310SR19   A0A0N8EER4   A0A0N8AW53   A0A0P5N6Y1   A0A0P5PE19   A0A0P6H880   A0A0P5NT52   A0A0P6FKB7   A0A0N8EK49   A0A0P6H7R4   E9GS46   A0A0P6GB56   A0A0P6BCV0   A0A1B6GXK8  

PDB

5V59     E-value=1.12176e-99,     Score=932

KEGG

101741795    K01872 alanyl-tRNA synthetase [EC:6.1.1.7] | (RefSeq) alanine--tRNA ligase, mitochondrial

PATHWAY

00970    Aminoacyl-tRNA biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0006419   GO:0004813   GO:0005737   GO:0003676   GO:0005524   GO:0000049   GO:0008270   GO:0002161   GO:0005739   GO:0006400   GO:0016597   GO:0006614   GO:0016021   GO:0000166   GO:0007154   GO:0016020   GO:0005112   GO:0015930  

Subcellular location

Mitochondrion  

Length:

997

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.14572

Exp number, first 60 AAs:

0.00719

Total prob of N-in:

0.00046

outside

1  -  997

 

 

Pi

266.766306

Theta

176.980987

Tajima's D

1.678806

CLR

0.051953

CSRT

0.820308984550773

Interpretation

Uncertain