Gene

KWMTBOMO14990

Pre Gene Modal

BGIBMGA004784

Annotation

PREDICTED:_protein_CLP1_homolog_[Amyelois_transitella]

Location

Bomo_Chr25(+):1925867-1932890      View in EpiBrowser

Full name

Protein CLP1 homolog       + More
Polyribonucleotide 5'-hydroxyl-kinase Clp1      

Alternative Name

Polyadenylation factor Clp1
Polynucleotide kinase Clp1
Pre-mRNA cleavage complex II protein Clp1

Location in the cell

Cytoplasmic   Reliability : 2.955

CDS

ATGACTGAAGTGCAACTACAAGAATTTAAGCTTGACCCCGATTCGGAATTACGTTTTGAAGTAGAATCGAAGAACGAAAAAGTCGTCTTAGAGGTAAAAAGTGGATATGCTGAATTATTCGGTACAGAACTGGTGAAAGGAAAGCCATACGAATTTCACACAGGAGCTAAAGTTGCTGTGTTTACTTGGCACGGATGTACTATAGAACTCAGAGGAAAGACTGAAGTCAGCTATGTAGCCAAAGAAACACCGATGATAGTGTACTTGAACGTACATGCGGCATTAGAACAGCAAAGAGTTGCAGCAGAACAAGAGAACACAAGAGGTCCGGTAACAATGATAGTTGGGCCTGCAGATGTTGGCAAGTCCACACTCTCAAGGATCCTTTTGAACTATGCTGTCCGCATGGGCAGGAGGCCTATTTATGTTGATTTGGATGTGGGACAGGGCCAGATTAGCGTTCCTGGTACAATCGGTGCATTGTTAGTAGAGCGCCCTGCGTCTATAGAAGAAGGCTTCAGTCAGCAGGCTCCGTTGGTTTATCATTTTGGTCACACTTCGCCGAGCGATAATATTGAGTTATACAACACCATTGTGTCGAGGCTAGCCGAAGTGATCACAGAGAAATGCGAAACGAATAAAAAAGCGGCGTCTTCGGGCGTCATAATAAACACGTGTGGATGGATTAAGAATGCTGGTTACAAGGTTTTAACTCATGCCGCTCAAGCATTTGAGGTTGACGTCATATTAGTTTTAGACAATGAGAGATTGTACAACGAGCTGAAGCGTGACATGCCTAAATTTGTGAAAGTAGTCTATCTACCTAAAAGTGGTGGGGTGGTGGAGCGCTCCGGCACGCAGAGGGCGGAGGCCCGGGACGCGCGCATCCGGGAGTACTTCTACGGGAAGAGAACACCATACTACCCGCATTCCTTTGATGTTAAATTCTCAGATCTCAAGATATACAAGGTGGGCGCGCCGTCGCTGCCGGACTCGTGCATGCCGCTGGGGATGCGCGCGGAGGACGCGCTGACGCGGCTGGTGGCGGTGTGGGGGGGCCGCGCGCTGCAGCACCGCCTGCTCGCCGTGTCCTTCGCGGCGGAGCCCGACGACCACGTGCTGCACTCCAACCTCGCGGGGTTCGTCTGTGTAACGGCAGTCGACATGGAGCGACAGACTTTGACGGTCCTGTCCCCGCAGCCGCGCCCTCTGCCCAACACGATCCTGTTGTTATCAGAACTACAATACATGGACAATCATTAG

Protein

MTEVQLQEFKLDPDSELRFEVESKNEKVVLEVKSGYAELFGTELVKGKPYEFHTGAKVAVFTWHGCTIELRGKTEVSYVAKETPMIVYLNVHAALEQQRVAAEQENTRGPVTMIVGPADVGKSTLSRILLNYAVRMGRRPIYVDLDVGQGQISVPGTIGALLVERPASIEEGFSQQAPLVYHFGHTSPSDNIELYNTIVSRLAEVITEKCETNKKAASSGVIINTCGWIKNAGYKVLTHAAQAFEVDVILVLDNERLYNELKRDMPKFVKVVYLPKSGGVVERSGTQRAEARDARIREYFYGKRTPYYPHSFDVKFSDLKIYKVGAPSLPDSCMPLGMRAEDALTRLVAVWGGRALQHRLLAVSFAAEPDDHVLHSNLAGFVCVTAVDMERQTLTVLSPQPRPLPNTILLLSELQYMDNH

Description

Required for endonucleolytic cleavage during polyadenylation-dependent pre-mRNA 3'-end formation.
Polynucleotide kinase that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. dsRNA is phosphorylated more efficiently than dsDNA, and the RNA component of a DNA:RNA hybrid is phosphorylated more efficiently than the DNA component. Appears to have roles in both tRNA splicing and mRNA 3'-end formation. Component of the tRNA splicing endonuclease complex. Phosphorylates the 5'-terminus of the tRNA 3'-exon during tRNA splicing; this phosphorylation event is a prerequisite for the subsequent ligation of the two exon halves and the production of a mature tRNA. Component of the pre-mRNA cleavage complex II (CF-II), which seems to be required for mRNA 3'-end formation. Also phosphorylates the 5'-terminus of exogenously introduced short interfering RNAs (siRNAs), which is a necessary prerequisite for their incorporation into the RNA-induced silencing complex (RISC). However, endogenous siRNAs and microRNAs (miRNAs) that are produced by the cleavage of dsRNA precursors by DICER1 already contain a 5'-phosphate group, so this protein may be dispensible for normal RNA-mediated gene silencing.

Catalytic Activity

5'-dephospho-(DNA) + ATP = ADP + H(+) + phospho-5'-(DNA)
5'-dephospho-(RNA) + ATP = 5'-end-(RNA) + ADP + H(+)

Subunit

Component of the tRNA splicing endonuclease complex. Component of pre-mRNA cleavage complex II (CF-II).

Similarity

Belongs to the Clp1 family. Clp1 subfamily.

Keywords

ATP-binding   Complete proteome   mRNA processing   Nucleotide-binding   Nucleus   Reference proteome  

Feature

chain  Protein CLP1 homolog

Uniprot

S4PM57   A0A2A4JSV0   A0A2H1V530   A0A194RI29   A0A194PH92   H9J5J4   + More
A0A212EUZ5   A0A067QNZ6   A0A2J7Q9P9   A0A3L8DBP0   A0A195FIC7   F4W693   A0A026WFV2   A0A151WHS8   A0A195AVL0   A0A158P214   A0A2A3E1S2   A0A088AW37   E9ISC1   A0A1B6EEL2   A0A0J7L6Z8   E2BIM6   A0A1B6BXL4   A0A1B6GLU8   A0A0C9R7A9   K7J1J2   A0A232FIT2   V5GVE7   A0A1E1X2W4   G3MND4   A0A023GK14   A0A131XH63   L7M8T0   A0A224YLY6   A0A131YSF0   A0A1Z5L6P2   A0A293MCY9   A0A1B0CM02   A0A1B0D1L0   A0A2R5LJW0   A0A0A9W5J4   A0A139WEM6   A0A1W4WBN3   A0A1Y1L955   E0VBS2   A0A0P4W2R7   A0A023F4J3   A0A224XPF8   A0A2R7VRS5   A0A069DU68   A0A182PA03   A0A182M913   B0VZR4   A0A0V0G7N5   A0A182WIY4   A0A182QEX3   A0A1Q3FCE4   A0A182RB20   A0A336M572   A0A182XZC6   A0A182UTN6   A0A336MQF3   A0A1C7CZJ7   A0A182UCF7   A0A182X6K8   A0A182I3V7   Q7QJW7   A0A182NHC8   U5EVR2   A0A182JCE3   A0A084VF65   A0A182JTV8   A0A2M4CTH4   A0A336MN00   A0A0K8WG81   A0A182FG29   A0A1B6EA97   A0A1A9W0M8   A0A0P5ZAU3   A0A034W9A0   A0A0A1WL03   A0A0N0BBL1   W8C415   D3TPC4   A0A1I8NFB6   A0A0L0BXL2   T1J8D4   E9FU50   Q16WA6   A0A2K7P6C0   A0A1I8QAR4   A0A1B0G3E5   A0A1D2NCD2   A0A1L8DGL6   A0A0P6IHX3   A0A087TNZ7   A0A182H1F3   A0A2I0TWQ1   G1NBF2   A0A226MWM2  

EC Number

2.7.1.78

Pubmed

23622113    26354079    19121390    22118469    24845553    30249741    + More
21719571    24508170    21347285    21282665    20798317    20075255    28648823    25765539    28503490    22216098    28049606    25576852    28797301    26830274    28528879    25401762    26823975    18362917    19820115    28004739    20566863    27129103    25474469    26334808    25244985    20966253    12364791    24438588    25348373    25830018    24495485    20353571    25315136    26108605    21292972    17510324    27289101    26483478    20838655   

EMBL

GAIX01000291    JAA92269.1    NWSH01000636    PCG75117.1    ODYU01000712    SOQ35941.1    + More
KQ460205    KPJ16985.1    KQ459604    KPI92074.1    BABH01025225    AGBW02012265    OWR45315.1    KK853123    KDR11058.1    NEVH01016344    PNF25313.1    QOIP01000010    RLU17299.1    KQ981523    KYN40425.1    GL887707    EGI70228.1    KK107262    EZA53904.1    KQ983112    KYQ47368.1    KQ976731    KYM76273.1    ADTU01006959    KZ288490    PBC25206.1    GL765324    EFZ16539.1    GEDC01000957    JAS36341.1    LBMM01000338    KMR00323.1    GL448516    EFN84473.1    GEDC01031548    JAS05750.1    GECZ01006371    JAS63398.1    GBYB01002641    JAG72408.1    NNAY01000155    OXU30493.1    GANP01010103    JAB74365.1    GFAC01005568    JAT93620.1    JO843385    AEO35002.1    GBBM01001187    JAC34231.1    GEFH01003565    JAP65016.1    GACK01004762    JAA60272.1    GFPF01006819    MAA17965.1    GEDV01007039    JAP81518.1    GFJQ02004246    JAW02724.1    GFWV01012281    MAA37010.1    AJWK01017982    AJVK01022110    GGLE01005706    MBY09832.1    GBHO01039892    GBRD01012824    GDHC01004401    JAG03712.1    JAG53002.1    JAQ14228.1    KQ971354    KYB26428.1    GEZM01066959    GEZM01066958    GEZM01066957    GEZM01066956    JAV67617.1    DS235035    EEB10828.1    GDKW01000792    JAI55803.1    GBBI01002565    JAC16147.1    GFTR01006106    JAW10320.1    KK854031    PTY09848.1    GBGD01001578    JAC87311.1    AXCM01001459    DS231815    GECL01002197    JAP03927.1    AXCN02000896    GFDL01009799    JAV25246.1    UFQS01000450    UFQT01000450    SSX04136.1    SSX24501.1    UFQT01001558    SSX31013.1    APCN01000225    AAAB01008807    GANO01000905    JAB58966.1    ATLV01012362    KE524785    KFB36609.1    GGFL01004401    MBW68579.1    SSX31011.1    GDHF01002217    JAI50097.1    GEDC01025302    GEDC01002461    JAS11996.1    JAS34837.1    GDIP01046258    LRGB01001937    JAM57457.1    KZS09962.1    GAKP01008050    JAC50902.1    GBXI01015092    JAC99199.1    KQ436240    KOX67341.1    GAMC01002537    JAC04019.1    EZ423276    ADD19552.1    JRES01001180    KNC24736.1    JH431954    GL732524    EFX89485.1    CH477572    CCAG010009527    LJIJ01000093    ODN02899.1    GFDF01008600    JAV05484.1    GDIQ01021713    JAN73024.1    KK116106    KFM66836.1    JXUM01023000    KQ560647    KXJ81453.1    KZ506809    PKU38237.1    MCFN01000375    OXB59727.1   

Proteomes

UP000218220    UP000053240    UP000053268    UP000005204    UP000007151    UP000027135    + More
UP000235965    UP000279307    UP000078541    UP000007755    UP000053097    UP000075809    UP000078540    UP000005205    UP000242457    UP000005203    UP000036403    UP000008237    UP000002358    UP000215335    UP000092461    UP000092462    UP000007266    UP000192223    UP000009046    UP000075885    UP000075883    UP000002320    UP000075920    UP000075886    UP000075900    UP000076408    UP000075903    UP000075882    UP000075902    UP000076407    UP000075840    UP000007062    UP000075884    UP000075880    UP000030765    UP000075881    UP000069272    UP000091820    UP000076858    UP000053105    UP000095301    UP000037069    UP000000305    UP000008820    UP000095300    UP000092444    UP000094527    UP000054359    UP000069940    UP000249989    UP000001645    UP000198323   

PRIDE

H9J5J4   

Pfam

PF06807   Clp1        + More
PF16575   CLP1_P
PF16573   CLP1_N
PF13516   LRR_6

Interpro

IPR027417   P-loop_NTPase        + More
IPR032324   Clp1_N       
IPR028606   Clp1       
IPR010655   Clp1_C       
IPR038239   Clp1_N_sf       
IPR032319   CLP1_P       
IPR038238   Clp1_C_sf       
IPR002156   RNaseH_domain       
IPR036397   RNaseH_sf       
IPR012337   RNaseH-like_sf       
IPR032675   LRR_dom_sf       
IPR001611   Leu-rich_rpt       

SUPFAM

SSF52540   SSF52540        + More
SSF53098   SSF53098       

Gene 3D

2.60.120.1030     3.30.70.2410     3.30.420.10     3.80.10.10    

ProteinModelPortal

S4PM57   A0A2A4JSV0   A0A2H1V530   A0A194RI29   A0A194PH92   H9J5J4   + More
A0A212EUZ5   A0A067QNZ6   A0A2J7Q9P9   A0A3L8DBP0   A0A195FIC7   F4W693   A0A026WFV2   A0A151WHS8   A0A195AVL0   A0A158P214   A0A2A3E1S2   A0A088AW37   E9ISC1   A0A1B6EEL2   A0A0J7L6Z8   E2BIM6   A0A1B6BXL4   A0A1B6GLU8   A0A0C9R7A9   K7J1J2   A0A232FIT2   V5GVE7   A0A1E1X2W4   G3MND4   A0A023GK14   A0A131XH63   L7M8T0   A0A224YLY6   A0A131YSF0   A0A1Z5L6P2   A0A293MCY9   A0A1B0CM02   A0A1B0D1L0   A0A2R5LJW0   A0A0A9W5J4   A0A139WEM6   A0A1W4WBN3   A0A1Y1L955   E0VBS2   A0A0P4W2R7   A0A023F4J3   A0A224XPF8   A0A2R7VRS5   A0A069DU68   A0A182PA03   A0A182M913   B0VZR4   A0A0V0G7N5   A0A182WIY4   A0A182QEX3   A0A1Q3FCE4   A0A182RB20   A0A336M572   A0A182XZC6   A0A182UTN6   A0A336MQF3   A0A1C7CZJ7   A0A182UCF7   A0A182X6K8   A0A182I3V7   Q7QJW7   A0A182NHC8   U5EVR2   A0A182JCE3   A0A084VF65   A0A182JTV8   A0A2M4CTH4   A0A336MN00   A0A0K8WG81   A0A182FG29   A0A1B6EA97   A0A1A9W0M8   A0A0P5ZAU3   A0A034W9A0   A0A0A1WL03   A0A0N0BBL1   W8C415   D3TPC4   A0A1I8NFB6   A0A0L0BXL2   T1J8D4   E9FU50   Q16WA6   A0A2K7P6C0   A0A1I8QAR4   A0A1B0G3E5   A0A1D2NCD2   A0A1L8DGL6   A0A0P6IHX3   A0A087TNZ7   A0A182H1F3   A0A2I0TWQ1   G1NBF2   A0A226MWM2  

PDB

4OI2     E-value=5.76489e-118,     Score=1086

KEGG

101736699    K14399 polyribonucleotide 5'-hydroxyl-kinase [EC:2.7.1.78] | (RefSeq) protein CLP1 homolog

PATHWAY

03015    mRNA surveillance pathway - Bombyx mori (domestic silkworm)

GO

GO:0005524   GO:0031124   GO:0005849   GO:0005634   GO:0016301   GO:0006378   GO:0006388   GO:0051736   GO:0035087   GO:0000214   GO:0046404   GO:0021695   GO:0030423   GO:0005829   GO:0016787   GO:0030163   GO:0008138   GO:0035091   GO:0007154   GO:0016020   GO:0005112   GO:0015930  

Subcellular location

Nucleus  

Length:

420

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00403999999999999

Exp number, first 60 AAs:

7e-05

Total prob of N-in:

0.00640

outside

1  -  420

 

 

Pi

275.346531

Theta

168.125978

Tajima's D

1.83429

CLR

0.406134

CSRT

0.856057197140143

Interpretation

Uncertain