Gene

KWMTBOMO14844

Pre Gene Modal

BGIBMGA012194

Annotation

protein_O-fucosyltransferase_2_precursor_[Bombyx_mori]

Location

Bomo_Chr24(-):16235151-16236580      View in EpiBrowser

Full name

GDP-fucose protein O-fucosyltransferase 2      

Alternative Name

Peptide-O-fucosyltransferase 2

Location in the cell

Cytoplasmic   Reliability : 1.674

CDS

ATGAAACTAATTTCCACAATATTTTTTAATTTAATACTGATAAACGCTGCAATTAATACTGAAAATGGTTACTGTGTATCAAAGGACTCTTGTGCGGAAACAAAAAATACATCTTCCGACAAAAAATGGTTATTCTACGACGTTAATCCACCCGAAGGGTTTAATTTGCGACGAGACGTGTACATGAGATTCGCTATATTACTCTCCAACGAACAGAAACGAGGTAAACTGAAAGAGTTCGGATTAGTTCTACCGCCTTGGTATAAGCTGTACCATTGGAAAACCACAAAAAAGAGAGAACCAATTCCCTGGGGAAATTTTTTCGATATAGAAAGCTTAAAATCATTCGCGCCTGTCGTTGAGTTGTACGAGATATTCGAAAAATCTCTACAAAAGCAATTGATAATAGATACGATTTACGTGTTGCAAAACTACGACAATCCCTTCGAAAATGGCCTTTTCGAAGACAAATGGGAGGTACAGGAGGAATGTCCGTACGTGCCACAGTACTGGGGCTACGGAAACATAACGGCCAACGAGATAATCTGTGTTAAATTTCAAGGAAAAATTTCCATGCTACCGGAACTTTTTCAATTACACCGTAATGACAAATTCATAATGGTGGCCCACGGTGAAATACCTCTACACGATAATTATGGCGACCGAAAATACTGGAAATGCAGGAAAAGTATGAAATTCAATAAGAAACTAACAGAAATTGCTTTTAATTACATTAAAGAAAAATTAAATTGTGATACAGAGCTGTGCAAAAACTATTTAGGCGTGCACTGGAGGAGACAGGATTTTGCTAAATACAGGAGCAAAGACGTACCAACAACGTCTGGAACGGTCAAGCAAATCCAAAACATAATGCAAAACAAACTTCCTCATGTGCAAACAGTTTTCATAGCTACAGACGCATCGGCCAAAGAAAGAGAAATACTCCAGAATAATCTAGAAACTTTAGGTTATAACGTACATTATTATGTACCTAATATAGCTGAAGTTACCGAATTTGATGATGGTACTATTGCGATATTAGAACAAATAATTTGTTCACATGCTGCCTTCTTTGTGGGCACACATGAAAGTACCTTTACATTCCGAATACAAGAGGAAAGGGAGTTATTAGGGTTTCCTAGTGATACTACGTTCAATAGGCTGTGCCCCGACAAAGGCAATTGCGAAAAGCCATCCCGTTGGACTGTAGTGAATTAA

Protein

MKLISTIFFNLILINAAINTENGYCVSKDSCAETKNTSSDKKWLFYDVNPPEGFNLRRDVYMRFAILLSNEQKRGKLKEFGLVLPPWYKLYHWKTTKKREPIPWGNFFDIESLKSFAPVVELYEIFEKSLQKQLIIDTIYVLQNYDNPFENGLFEDKWEVQEECPYVPQYWGYGNITANEIICVKFQGKISMLPELFQLHRNDKFIMVAHGEIPLHDNYGDRKYWKCRKSMKFNKKLTEIAFNYIKEKLNCDTELCKNYLGVHWRRQDFAKYRSKDVPTTSGTVKQIQNIMQNKLPHVQTVFIATDASAKEREILQNNLETLGYNVHYYVPNIAEVTEFDDGTIAILEQIICSHAAFFVGTHESTFTFRIQEERELLGFPSDTTFNRLCPDKGNCEKPSRWTVVN

Description

Catalyzes the reaction that attaches fucose through an O-glycosidic linkage to a conserved serine or threonine residue in the consensus sequence C1-X(2,3)-S/T-C2-X(2)-G of thrombospondin type 1 repeats where C1 and C2 are the first and second cysteines, respectively. O-fucosylates members of several protein families including the ADAMTS family, the thrombosporin (TSP) and spondin families. The O-fucosylation of TSRs is also required for restricting epithelial to mesenchymal transition (EMT), maintaining the correct patterning of mesoderm and localization of the definite endoderm (By similarity). Required for the proper secretion of ADAMTS family members such as ADAMSL1 and ADAMST13.

Catalytic Activity

Transfers an alpha-L-fucosyl residue from GDP-beta-L-fucose to the serine hydroxy group of a protein acceptor.

Biophysicochemical Properties

9.8 uM for GDP-fucose

Similarity

Belongs to the glycosyltransferase 68 family.

Keywords

3D-structure   Alternative splicing   Carbohydrate metabolism   Complete proteome   Disulfide bond   Endoplasmic reticulum   Fucose metabolism   Glycoprotein   Glycosyltransferase   Golgi apparatus   Reference proteome   Signal   Transferase  

Feature

chain  GDP-fucose protein O-fucosyltransferase 2
splice variant  In isoform B.

Uniprot

Q00P36   Q659L5   A0A2A4KA84   A0A2W1BBV8   A0A0N0PDQ9   S4PXP5   + More
A0A194Q0Z0   A0A212F2S7   A0A212F2Q4   D6WRI8   A0A1Y1MFF9   R4FP67   E0VEA7   A0A2P8XHD8   A0A1W4WSE4   J3JY99   A0A0P4VST8   A0A151WTF1   A0A158NRJ4   A0A154PIX3   E2C3D5   A0A151I8Y5   A0A026VZH5   A0A088ARB6   A0A146LL86   A0A0L7RCT4   A0A0A9WW19   A0A0P5PQ29   A0A0P5PMB5   A0A195BU85   A0A0N0BG72   A0A2P6KD75   A0A0P6FIZ7   A0A0P5RLY4   A0A182HBG0   V5GHC5   A0A1B6FEF3   V5II81   A0A2A3EBZ0   A0A224Z5R1   A0A0N8A3E4   A0A0P5VY32   A0A0P5B3V9   A0A0P6AIM2   A0A2C9JSB3   A0A131XSF2   L7M5C6   A0A164YLQ4   A0A3B3IBZ2   A0A3S1AQR3   A0A131YYT0   A0A3P9LMZ7   A0A0P5LST5   A0A1S4F6U9   Q0IFR1   C4WX22   A0A0P5AYQ8   A0A3P9H4A4   A0A0A1WW97   Q00P34   A0A3Q1B5L0   A0A3P8T7D1   A0A3Q2FYF9   A0A0P5AQP2   A0A2S2QIT1   A0A3B5K6P6   N6UR66   U4UB77   A0A0L0C8S0   F1L550   A0A067R730   A0A0P5R135   E9GXZ5   A0A0P5WUF9   A0A3Q1F6I5   A0A146NVL5   W8C4I8   A0A182IRA3   A0A3P8QBR5   A0A3B3B4N8   A0A3B4BE28   A0A2K5WB24   A0A087T4T4   K1P9F9   A0A3B4GWF5   A0A3Q2UU62   A0A3B3UQ17   A0A087X929   A0A2Y9E0H5   A0A2Y9G6Z9   I0FGL4   A0A182E546   A0A0S7H6B7   A0A044TK33   Q9Y2G5   A0A3Q2P4I9   G1QQA2   Q00P33   B0S641  

EC Number

2.4.1.221

Pubmed

16679357    19121390    28756777    26354079    23622113    22118469    + More
18362917    19820115    28004739    20566863    29403074    22516182    27129103    21347285    20798317    24508170    30249741    26823975    25401762    26483478    25765539    28797301    15562597    25576852    17554307    26830274    17510324    25830018    21551351    23537049    26108605    21685128    24845553    21292972    24495485    29451363    25463417    22992520    25319552    22919073    15233996    12966037    10231032    17974005    10830953    15489334    11067851    16464858    17395589    17395588    25944712    22588082    23594743   

EMBL

BABH01031823    DQ139953    ABA29474.1    AJ831834    CAH41977.1    NWSH01000015    + More
PCG80824.1    KZ150242    PZC71901.1    KQ460240    KPJ16389.1    GAIX01004678    JAA87882.1    KQ459580    KPI99252.1    AGBW02010693    OWR48021.1    OWR48020.1    KQ971351    EFA07652.2    GEZM01035974    GEZM01035973    GEZM01035972    GEZM01035971    JAV83065.1    GAHY01001034    JAA76476.1    DS235088    EEB11713.1    PYGN01002122    PSN31421.1    BT128223    AEE63184.1    GDKW01001552    JAI55043.1    KQ982757    KYQ51113.1    ADTU01002058    ADTU01002059    KQ434931    KZC11772.1    GL452320    EFN77473.1    KQ978331    KYM95069.1    KK107538    QOIP01000005    EZA49132.1    RLU22922.1    GDHC01009911    JAQ08718.1    KQ414615    KOC68600.1    GBHO01030958    GBHO01030957    GBRD01007399    GBRD01007398    JAG12646.1    JAG12647.1    JAG58422.1    GDIQ01146128    JAL05598.1    GDIQ01147518    JAL04208.1    KQ976403    KYM92137.1    KQ435791    KOX74368.1    MWRG01015540    PRD24271.1    GDIQ01063664    JAN31073.1    GDIQ01098847    JAL52879.1    JXUM01124797    KQ566870    KXJ69771.1    GANP01014848    JAB69620.1    GECZ01021285    GECZ01020965    JAS48484.1    JAS48804.1    GANP01003647    JAB80821.1    KZ288289    PBC29293.1    GFPF01010456    MAA21602.1    GDIP01186385    JAJ37017.1    GDIP01093592    JAM10123.1    GDIP01190095    JAJ33307.1    GDIP01030105    JAM73610.1    GEFM01006661    JAP69135.1    GACK01006735    JAA58299.1    LRGB01000872    KZS15384.1    RQTK01001694    RUS69404.1    GEDV01004845    JAP83712.1    GDIQ01165773    JAK85952.1    CH477294    EAT44474.1    ABLF02038228    AK342404    BAH72442.1    GDIP01196548    JAJ26854.1    GBXI01010993    JAD03299.1    DQ139955    ABA29476.1    GDIP01195437    JAJ27965.1    GGMS01008400    MBY77603.1    APGK01019954    KB740150    ENN81252.1    KB632173    ERL89578.1    JRES01000754    KNC28671.1    JI171547    ADY45254.1    KK852657    KDR19215.1    GDIQ01109391    JAL42335.1    GL732574    EFX75507.1    GDIP01081549    JAM22166.1    GCES01150539    JAQ35783.1    GAMC01002282    JAC04274.1    AQIA01044232    AQIA01044233    KK113396    KFM60123.1    JH818836    EKC20412.1    AYCK01006445    AYCK01006446    JV043519    AFI33590.1    UYRW01000565    VDK68049.1    GBYX01445506    GBYX01445502    JAO35931.1    CMVM020000146    AJ302080    AJ302079    AY066015    AJ575591    AB023175    AL110285    AL163301    BC011044    BC064623    ADFV01100857    ADFV01100858    DQ139956    ABA29477.1    BX323586    BC163087    BC163097    AAI63087.1   

Proteomes

UP000005204    UP000218220    UP000053240    UP000053268    UP000007151    UP000007266    + More
UP000009046    UP000245037    UP000192223    UP000075809    UP000005205    UP000076502    UP000008237    UP000078542    UP000053097    UP000279307    UP000005203    UP000053825    UP000078540    UP000053105    UP000069940    UP000249989    UP000242457    UP000076420    UP000076858    UP000001038    UP000271974    UP000265180    UP000008820    UP000007819    UP000265200    UP000257160    UP000265080    UP000265020    UP000005226    UP000019118    UP000030742    UP000037069    UP000027135    UP000000305    UP000257200    UP000075880    UP000265100    UP000261560    UP000261520    UP000233100    UP000054359    UP000005408    UP000261460    UP000264840    UP000261500    UP000028760    UP000248480    UP000248481    UP000077448    UP000271087    UP000024404    UP000005640    UP000265000    UP000001073    UP000000437   

PRIDE

Q00P36    Q659L5    Q9Y2G5   

Pfam

PF10250   O-FucT

Interpro

IPR019378   GDP-Fuc_O-FucTrfase       

ProteinModelPortal

Q00P36   Q659L5   A0A2A4KA84   A0A2W1BBV8   A0A0N0PDQ9   S4PXP5   + More
A0A194Q0Z0   A0A212F2S7   A0A212F2Q4   D6WRI8   A0A1Y1MFF9   R4FP67   E0VEA7   A0A2P8XHD8   A0A1W4WSE4   J3JY99   A0A0P4VST8   A0A151WTF1   A0A158NRJ4   A0A154PIX3   E2C3D5   A0A151I8Y5   A0A026VZH5   A0A088ARB6   A0A146LL86   A0A0L7RCT4   A0A0A9WW19   A0A0P5PQ29   A0A0P5PMB5   A0A195BU85   A0A0N0BG72   A0A2P6KD75   A0A0P6FIZ7   A0A0P5RLY4   A0A182HBG0   V5GHC5   A0A1B6FEF3   V5II81   A0A2A3EBZ0   A0A224Z5R1   A0A0N8A3E4   A0A0P5VY32   A0A0P5B3V9   A0A0P6AIM2   A0A2C9JSB3   A0A131XSF2   L7M5C6   A0A164YLQ4   A0A3B3IBZ2   A0A3S1AQR3   A0A131YYT0   A0A3P9LMZ7   A0A0P5LST5   A0A1S4F6U9   Q0IFR1   C4WX22   A0A0P5AYQ8   A0A3P9H4A4   A0A0A1WW97   Q00P34   A0A3Q1B5L0   A0A3P8T7D1   A0A3Q2FYF9   A0A0P5AQP2   A0A2S2QIT1   A0A3B5K6P6   N6UR66   U4UB77   A0A0L0C8S0   F1L550   A0A067R730   A0A0P5R135   E9GXZ5   A0A0P5WUF9   A0A3Q1F6I5   A0A146NVL5   W8C4I8   A0A182IRA3   A0A3P8QBR5   A0A3B3B4N8   A0A3B4BE28   A0A2K5WB24   A0A087T4T4   K1P9F9   A0A3B4GWF5   A0A3Q2UU62   A0A3B3UQ17   A0A087X929   A0A2Y9E0H5   A0A2Y9G6Z9   I0FGL4   A0A182E546   A0A0S7H6B7   A0A044TK33   Q9Y2G5   A0A3Q2P4I9   G1QQA2   Q00P33   B0S641  

PDB

4AP5     E-value=2.64832e-68,     Score=657

KEGG

692681    K03691 peptide-O-fucosyltransferase [EC:2.4.1.221] | (RefSeq) pofut2, Fut13, O-FucT-2; protein O-fuc

PATHWAY

00514    Other types of O-glycan biosynthesis - Bombyx mori (domestic silkworm)

GO

GO:0016757   GO:0006004   GO:0046922   GO:0008107   GO:0016021   GO:0010468   GO:0010717   GO:0051046   GO:0005794   GO:0001707   GO:0036066   GO:0005789  

Subcellular location

Endoplasmic reticulum  
Golgi apparatus  

SignalP

Position:   1 - 16,         Likelihood:  0.952763

 

 

Length:

405

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0753699999999999

Exp number, first 60 AAs:

0.06792

Total prob of N-in:

0.00563

outside

1  -  405

 

 

Pi

139.161835

Theta

148.92865

Tajima's D

-0.217927

CLR

67.890534

CSRT

0.310534473276336

Interpretation

Uncertain