SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO14778
Pre Gene Modal
BGIBMGA003841
Annotation
PREDICTED:_LOW_QUALITY_PROTEIN:_BRCA1-associated_RING_domain_protein_1-like_[Bombyx_mori]
Full name
BRCA1-associated RING domain protein 1      
Alternative Name
RING-type E3 ubiquitin transferase BARD1
Location in the cell
Nuclear   Reliability : 3.37
 

Sequence

CDS
ATGTCTTCTAAAGACTTAAATAACTTCTTAAAAGCATTGGACGCAGTCAAAGAAGATTACACTTGCGGCGTCTGTTCAGAACTCTGTAAAAATCCAGTGACTTTGAGTAAATGTTTTCATTTGATATGCGCTAGTCACTTCGAGGGCTTGGACGCTTGTCCGACCTGCGGGACGAATTTAGAAGGTTGCAAAACCTTCACCGACGAACGTCTTGCCGTATGCATAAACTCGGCGAATAAATTAGACGAGATATTCAGTTGTTTTAGATCTAAAGAAACGTCTACGACAAAACAAGATAAAATCGAATCTAACAGTAAAACCACGTTAACAGATGTCCGAAAACCTCAGGCAAAGCTCCGAAGCCACGATATTTCAATAGCCAGCAGTAAATTCAGCAAGTCCAGTGAAAAACGCAATAATAAAGGTGAAACGCCACTACACATCGCCTGCAGGTTAGGTAAAGTTGACAAAATCACCGAACTATTAAACCAGGGCGTCAATACGAACACTAAAGACAATGCCGGTTGGACGCCTTTACATGAAGTTGTTCAAAATGGACGACTCGACTTGGTCACTCTTTTACTCCAATATAACACTTTAATTGACGTGCCGGGGCTCAATAATGAGACACCATTGCATGAAGCAGTCAGATACGGACACCGAGAAATCATTGAAGAACTTGTCAAACATGGAGCGGACATAAACTGTAGGAATTCTAAAGGGGAAACTCCACTGCAGCTAGCTACAGATGACTTGAAAATAATATTAAAGAAGGCATCAGAGAGTTTGATTCAAACACAGTCCGTTAATGTGACTCACATGTCGATCTTGCACTCAGAGTTAGATTTTGATGATATAAGAGTCTATTGTGTATCTCAGCAGAAGTCGGTTGTATCAAAACTAAAATTGCTTTGTAAACAACACAGCAATTTGCATATTGAAGTCAAATTTACAAAAAAAGTAACACATTTGATCGTTGATGCCGAGAATGGTATATGTATGTCGACAATAGATGTACTGCAGGGGATCGTGAATTCCATATGGATATTAACGTCAGATTGGGTCCTGAAATCTACAGAAGATAGTTTGGAACAGTTTGAACAATTTGAGGTTACTGGAGTGGGTACAGCATCCTACAACGGTCCTAAAACAGCTCGTTATAACAAATACAAGCAACTACCAGGAGTGTTTGATGGCTGCCACTTCTATCTACACAATTTCAATATGAAATACGAAATATCCAAGCACCTGGTGCTGACCAAAGCGACTATAAGCAAGCTGATTACTGATGCAGGCGGTATAGTGCTCAGGAGAGTGCCCAACCCGGAATCCATACCGGACCAAGAGAAATTGGTGCCGTATCATGCTAAAAAAGATGGAAAACTATATATTTGTTCGCATTATATAATTTTTAAAGACACGTACCAGCCAATGTATAATATGCAGCATCTTAAAGCGCTGCCAATAGGCTGGTTGATTGAGTGCATAGAGAGATATGAACTGTGTGATCCGTGA
Protein
MSSKDLNNFLKALDAVKEDYTCGVCSELCKNPVTLSKCFHLICASHFEGLDACPTCGTNLEGCKTFTDERLAVCINSANKLDEIFSCFRSKETSTTKQDKIESNSKTTLTDVRKPQAKLRSHDISIASSKFSKSSEKRNNKGETPLHIACRLGKVDKITELLNQGVNTNTKDNAGWTPLHEVVQNGRLDLVTLLLQYNTLIDVPGLNNETPLHEAVRYGHREIIEELVKHGADINCRNSKGETPLQLATDDLKIILKKASESLIQTQSVNVTHMSILHSELDFDDIRVYCVSQQKSVVSKLKLLCKQHSNLHIEVKFTKKVTHLIVDAENGICMSTIDVLQGIVNSIWILTSDWVLKSTEDSLEQFEQFEVTGVGTASYNGPKTARYNKYKQLPGVFDGCHFYLHNFNMKYEISKHLVLTKATISKLITDAGGIVLRRVPNPESIPDQEKLVPYHAKKDGKLYICSHYIIFKDTYQPMYNMQHLKALPIGWLIECIERYELCDP

Summary

Description
E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.
Catalytic Activity
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.
Subunit
Homo- and heterodimer. Heterodimer (RING-type zinc finger) with BRCA1. Heterodimer (via ANK repeats and BRCT domains) with CSTF1/CSTF-50. Component of the BRCA1-A complex, at least composed of the BRCA1, BARD1, UIMC1/RAP80, ABRAXAS1, BRCC3/BRCC36, BABAM2 and BABAM1/NBA1. Interacts with UBXN1.
Keywords
3D-structure   Alternative splicing   ANK repeat   Complete proteome   DNA damage   DNA repair   Isopeptide bond   Metal-binding   Nucleus   Phosphoprotein   Polymorphism   Reference proteome   Repeat   Transferase   Ubl conjugation   Ubl conjugation pathway   Zinc   Zinc-finger  
Feature
chain  BRCA1-associated RING domain protein 1
splice variant  In isoform beta.
sequence variant  Common polymorphism in Caucasians; less frequent in Africans; dbSNP:rs1048108.
EC Number
2.3.2.27
EMBL
BABH01036991    BABH01036992    BABH01036993    BABH01036994    JTDY01001215    KOB74511.1    + More
KZ150174    PZC72577.1    NWSH01002862    PCG67424.1    ODYU01002408    SOQ39846.1    KQ460246    KPJ16376.1    KQ460254    KPJ16345.1    KQ459580    KPI99227.1    FX985864    BBA93751.1    NEVH01025127    PNF16090.1    KK852463    KDR23353.1    KQ976725    KYM76519.1    KQ435007    KZC13413.1    ADTU01016131    ADTU01016132    QOIP01000006    RLU21993.1    KQ981763    KYN36085.1    GL888255    EGI63769.1    GDIP01211183    GDIP01160679    JAJ12219.1    JAJ62723.1    GDIP01211182    GDIP01163090    JAJ60312.1    GDIP01092186    JAM11529.1    GDIP01163091    JAJ60311.1    GDIP01051542    JAM52173.1    GDIP01052739    JAM50976.1    GDIP01125336    GDIP01124327    JAL78378.1    GDIP01120077    JAL83637.1    GDIP01196532    GDIP01155184    JAJ26870.1    GDIP01146342    GDIP01074646    JAJ77060.1    GDIP01177095    JAJ46307.1    GDIP01074645    JAM29070.1    GDIP01161387    GDIP01131171    JAL72543.1    GDIP01177094    JAJ46308.1    GDIQ01257972    JAJ93752.1    GDIP01162452    JAJ60950.1    GDIP01097394    JAM06321.1    GDIP01168883    JAJ54519.1    GDIP01064334    JAM39381.1    GDIP01156550    JAJ66852.1    GDIP01081200    JAM22515.1    LRGB01002066    KZS09467.1    GDIP01078697    JAM25018.1    GDIP01113084    JAL90630.1    GDIP01085589    JAM18126.1    GDIP01089088    JAM14627.1    GDIP01084143    JAM19572.1    GDIP01087979    JAM15736.1    GDIP01086202    JAM17513.1    GDIP01018468    JAM85247.1    GL732541    EFX81945.1    GDIP01241184    JAI82217.1    GDIP01241183    JAI82218.1    GDIP01137684    JAL66030.1    KK107128    EZA58385.1    KQ977901    KYM98879.1    GL768153    EFZ11954.1    KZ288301    PBC28860.1    DS235231    EEB13686.1    LBMM01001319    KMQ96512.1    KMQ96513.1    RQTK01001154    RUS71816.1    GGMR01002317    MBY14936.1    GDIP01007433    JAM96282.1    GDIP01046307    JAM57408.1    PNF16091.1    AAPE02003325    AAPE02003326    AAPE02003327    KQ982813    KYQ50255.1    KQ414588    KOC70454.1    KE164381    EPQ17669.1    PZQS01000005    PVD30849.1    KQ978957    KYN27180.1    GDIP01126832    JAL76882.1    ABLF02024952    HACA01005611    CDW22972.1    LSMT01000279    PFX21365.1    BC126428    AAI26429.1    AK314260    BAG36925.1    AGTP01068239    AGTP01068240    AJFE02039920    AJFE02039921    AJFE02039922    AK223409    BAD97129.1    U76638    AF038042    AF038034    AF038035    AF038036    AF038037    AF038038    AF038039    AF038040    AF038041    JF790280    JF790281    JF790282    AC016708    BC126426    CABD030018551    CABD030018552    AANG04002752    ABGA01138559    ABGA01138560    ABGA01138561    ABGA01138562    NDHI03003547    PNJ24152.1   
Pfam
PF00405   Transferrin        + More
PF00023   Ank
PF12796   Ank_2
PF13606   Ank_3
PF00533   BRCT
PF16589   BRCT_2
PF14835   zf-RING_6
PF00005   ABC_tran
Interpro
IPR002110   Ankyrin_rpt        + More
IPR001156   Transferrin-like_dom       
IPR001357   BRCT_dom       
IPR036420   BRCT_dom_sf       
IPR036770   Ankyrin_rpt-contain_sf       
IPR020683   Ankyrin_rpt-contain_dom       
IPR013083   Znf_RING/FYVE/PHD       
IPR017907   Znf_RING_CS       
IPR001841   Znf_RING       
IPR039503   BARD1_Znf-RING       
IPR030371   ABCA12       
IPR026082   ABCA       
IPR003439   ABC_transporter-like       
IPR027417   P-loop_NTPase       
IPR003593   AAA+_ATPase       
SUPFAM
SSF48403   SSF48403        + More
SSF52113   SSF52113       
SSF52540   SSF52540       
Gene 3D
PDB
6MOL     E-value=1.70253e-22,     Score=263

Ontologies

Topology

Subcellular location
Nucleus  
Length:
504
Number of predicted TMHs:
0
Exp number of AAs in TMHs:
0.00174
Exp number, first 60 AAs:
0
Total prob of N-in:
0.00107
outside
1  -  504
 
 

Population Genetic Test Statistics

Pi
193.702331
Theta
150.60651
Tajima's D
0.911895
CLR
1.060182
CSRT
0.638818059097045
Interpretation
Uncertain
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