Gene

KWMTBOMO14699  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009571

Annotation

Receptor-interacting_serine/threonine-protein_kinase_4_[Papilio_machaon]

Location

Bomo_Chr24(-):12937538-12943303      View in EpiBrowser

Full name

Histone-lysine N-methyltransferase EHMT1      

Alternative Name

Euchromatic histone-lysine N-methyltransferase 1
G9a-like protein 1
Lysine N-methyltransferase 1D

Location in the cell

Cytoplasmic   Reliability : 1.335 Extracellular   Reliability : 1.02 Nuclear   Reliability : 1.56

CDS

ATGTGTTCCGAAGCAGAGAAAGCTCAGTTGTTTGAAGCTATCGACAACAATAATGTAGAAAAAGCTGACGTACTTCTATCTTCAACTGTGGACGTTAACGTACGGAGTCTCGAAGTGAAGAATCGTACTGCGCTGCACCTGGCAGCCCACGCGGGGCGCTACGACCTAGTCGCTCTGCTAGTCGACAAACACAAAGCAGACGTCAACATCGAAGACAGCGAAGGCGACATCCCCTTGCAGCTAGCCACAGATCGCCATCATATGAAGATAGTCAAGTTCCTCCTAAGCAGAAACTCCAAAGGGCGTGAGTACGCGGCGCATTTCCTAAACACTGACGAAGTAGAGGAATTCTTCCAAGCGGCCAAAGCGAATGACATCCAGAGAGTGACGGAGCTACTCGACAGTGGTGTGGATGTCAATTCGGTAGATTTGTCTGATCTACACCGATCCGCGTTGCATGTTGCAGCTCGGGAAGGTCATTATGATCTAGTGCAACTTCTCCTGGAGCGTGGTGCTGATATGCAATACGAAGACGACACTGATGAGTGCGCCTACCACATAGCCCTTAATAACGACCACGACAATATAGGCAGTTTGCTCTTAAAAACAGGGTATATTCCCGACCCGCGCGACGACTCCAGTGTAAGCAGTGACACTGATGAAAGTGTAAGCTTAAGCTTTCTTTTTAACTAA

Protein

MCSEAEKAQLFEAIDNNNVEKADVLLSSTVDVNVRSLEVKNRTALHLAAHAGRYDLVALLVDKHKADVNIEDSEGDIPLQLATDRHHMKIVKFLLSRNSKGREYAAHFLNTDEVEEFFQAAKANDIQRVTELLDSGVDVNSVDLSDLHRSALHVAAREGHYDLVQLLLERGADMQYEDDTDECAYHIALNNDHDNIGSLLLKTGYIPDPRDDSSVSSDTDESVSLSFLFN

Description

Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin. H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones. Also weakly methylates 'Lys-27' of histone H3 (H3K27me). Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently. Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1. During G0 phase, it probably contributes to silencing of MYC- and E2F-responsive genes, suggesting a role in G0/G1 transition in cell cycle. In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys-373' of p53/TP53.

Catalytic Activity

L-lysyl-[histone] + S-adenosyl-L-methionine = H(+) + N(6)-methyl-L-lysyl-[histone] + S-adenosyl-L-homocysteine

Subunit

Interacts with WIZ. Part of the E2F6.com-1 complex in G0 phase composed of E2F6, MGA, MAX, TFDP1, CBX3, BAT8, EHMT1, RING1, RNF2, MBLR, L3MBTL2 and YAF2. Interacts with MPHOSPH8 (By similarity). Interacts with CDYL. Interacts with REST only in the presence of CDYL. Part of a complex containing at least CDYL, REST, WIZ, SETB1, EHMT1 and EHMT2 (By similarity). Heterodimer; heterodimerizes with EHMT2. Interacts (via ANK repeats) with RELA (when monomethylated at 'Lys-310').

Similarity

Belongs to the class V-like SAM-binding methyltransferase superfamily.

Keywords

Acetylation   Alternative splicing   ANK repeat   Chromatin regulator   Chromosome   Complete proteome   Isopeptide bond   Metal-binding   Methyltransferase   Nucleus   Phosphoprotein   Reference proteome   Repeat   S-adenosyl-L-methionine   Transferase   Ubl conjugation   Zinc  

Feature

chain  Histone-lysine N-methyltransferase EHMT1
splice variant  In isoform 2.

Uniprot

H9JJ70   A0A194RW00   A0A194Q060   A0A154NY84   A0A090MA93   A0A087V491   + More
B3EU24   C3NIT9   X1H8V7   A0A1F9AH59   A0A2P6KIZ5   A0A0P7V8U0   A0A3M0IXE2   U3JSN1   I4BZW7   A0A0B8N264   A0A091SQI4   A0A1W4YJ43   A0A225V0M0   H0YXA8   A0A1S3GA49   W5M0N2   A0A218UDV4   A0A1S3G7V3   A0A1S3G885   A0A2S7Q6R5   A0A093SJC5   U3IXU8   A0A3B0YXP3   G2Y6T1   A0A384J8K7   M7TUB6   A0A091IZR3   A0A091QV00   A0A3Q0H4H9   A0A1V4J783   A0A287DCB6   R0L5Y1   A0A3Q0H944   I3LWJ6   A0A091CQK6   A0A1V4J7B8   A0A2K6GI19   Q47DZ9   A0A2J8RMH6   Q4EC44   L8HI15   Q8BHB2   A0A1V3FVR2   A0A2J8PWU5   A0A1B0GWF6   F6UJH7   A0A1D5PVY2   A0A3Q0H4J8   G3UQS6   G3WHW7   A0A3Q0H949   A0A3Q0H964   A0A3L7HD24   A0A3Q0H4I9   A0A1D5PW08   G5BG04   C0R391   Z4YJZ7   A0A1D5PJ82   A0A151MF60   Q5DW34-2   A0A1U7SD70   A0A0H2UH19   A0A250Y7B8   A0A151MF43   Q5DW34   K7FDM7   G3I5Z2   A0A151MFI4   A0A0P6J5K3   A0A3L8SDY2   H9GIS7   K7FDN4   Q73I01   A0A3Q2U3H4   A0A226PMU1   A0A1D5PIX7   A0A3Q0H5S0   A0A151MF76   A0A0Q3MA63   E9Q5A3   H2R5Y8   D4A005   A0A226NG00   A0A1A6GSP9   H0V8M4   H7CHP8   G1RDJ8   Q5DW34-3   G1MU25   A0A286XW35   A0A1D5P7T6   Q5F3H1   A0A1D5P0G7  

Pubmed

19121390    26354079    20023027    19435847    24624126    27774985    + More
20360741    23749191    21876677    23104368    26913498    23704180    19650930    15774024    23375108    10349636    11042159    11076861    11217851    12466851    16141073    15164053    21406692    23186163    25755297    28112733    17495919    15592404    20838655    21709235    29704459    21993625    19307581    19468303    22293439    15774718    15489334    18818694    21131967    28087693    17381049    21804562    30282656    21881562    15024419    24621616    16136131    15057822    21993624    15642098   

EMBL

BABH01036440    KQ459700    KPJ20366.1    KQ459582    KPI98946.1    KQ434782    + More
KZC04541.1    CBMG010003847    CEG04029.1    KL479275    KFO07433.1    CP001102    ACE06726.1    CP001404    ACP49049.1    BARU01029347    GAH65832.1    MGQK01000096    OGP57425.1    MWRG01010813    PRD26298.1    JARO02002970    KPP71466.1    QRBI01000209    RMB93514.1    AGTO01021651    CP003360    AFM22858.1    DF933843    GAM43281.1    KK931406    KFQ45237.1    NBNE01009010    OWY98851.1    ABQF01048574    ABQF01048575    ABQF01048576    ABQF01048577    ABQF01048578    ABQF01048579    ABQF01048580    AHAT01021783    MUZQ01000386    OWK51903.1    NJPR01000087    PQE22229.1    KL671500    KFW82784.1    ADON01124535    UOFK01000175    VAW78959.1    FQ790293    CCD48333.1    CP009806    ATZ46664.1    KB707926    EMR84885.1    KL218434    KFP05071.1    KK804840    KFQ31380.1    LSYS01008925    OPJ67914.1    AGTP01090670    AGTP01090671    KB744204    EOA95687.1    KN124375    KFO21439.1    OPJ67915.1    CP000089    AAZ46932.1    NDHI03003669    PNJ09655.1    AAGB01000025    EAL59018.1    KB007840    ELR24041.1    AK049454    AK052174    AK082062    BAC33756.1    BAC34869.1    BAC38402.1    MQAD01000002    OOE05664.1    NBAG03000141    PNI88490.1    AL365502    AL590627    AL611925    AADN05000150    AEFK01023222    AEFK01023223    AEFK01023224    AEFK01023225    AEFK01023226    AEFK01023227    AEFK01023228    AEFK01023229    AEFK01023230    AEFK01023231    RAZU01000235    RLQ63844.1    JH170063    EHB08215.1    CP001391    ACN95383.1    AL732525    AKHW03006215    KYO23149.1    AB205007    BC056938    BC089302    GFFV01000418    JAV39527.1    KYO23148.1    AGCU01190420    AGCU01190421    AGCU01190422    AGCU01190423    AGCU01190424    AGCU01190425    AGCU01190426    AGCU01190427    AGCU01190428    AGCU01190429    JH001326    EGW07293.1    KYO23150.1    GEBF01005764    JAN97868.1    QUSF01000027    RLW00329.1    AAWZ02030207    AAWZ02030208    AAWZ02030209    AE017196    AAS14111.1    AWGT02000046    OXB80677.1    KYO23151.1    LMAW01002595    KQK79328.1    AACZ04062180    AACZ04062181    AACZ04062182    AACZ04062183    AACZ04062184    AACZ04062185    AABR07051230    AABR07051231    AABR07051232    AABR07051233    AABR07051234    AABR07051235    AABR07051236    MCFN01000062    OXB66474.1    LZPO01075853    OBS68944.1    AAKN02024151    AAKN02024152    AAKN02024153    AAKN02024154    AAKN02024155    AB702942    BAL72057.1    ADFV01120965    ADFV01120966    ADFV01120967    ADFV01120968    ADFV01120969    ADFV01120970    ADFV01120971    ADFV01120972    ADFV01120973    ADFV01120974    AJ851679    CAH65313.1   

Proteomes

UP000005204    UP000053240    UP000053268    UP000076502    UP000001227    UP000006818    + More
UP000034805    UP000269221    UP000016665    UP000006055    UP000192224    UP000198211    UP000007754    UP000081671    UP000018468    UP000197619    UP000238813    UP000053258    UP000016666    UP000008177    UP000001798    UP000012045    UP000054308    UP000189705    UP000190648    UP000005215    UP000028990    UP000233160    UP000000550    UP000011083    UP000188458    UP000005640    UP000002280    UP000000539    UP000001645    UP000007648    UP000273346    UP000006813    UP000001293    UP000000589    UP000050525    UP000007267    UP000001075    UP000276834    UP000001646    UP000008215    UP000198419    UP000051836    UP000002277    UP000002494    UP000198323    UP000092124    UP000005447    UP000001073   

PRIDE

H9JJ70    Q8BHB2    Z4YJZ7    Q5DW34    A0A0H2UH19    G3I5Z2    + More
E9Q5A3    D4A005    H7CHP8    Q5F3H1   

Pfam

PF12796   Ank_2        + More
PF01753   zf-MYND
PF13606   Ank_3
PF05033   Pre-SET
PF00856   SET
PF04564   U-box
PF00023   Ank
PF17107   SesA

Interpro

IPR002110   Ankyrin_rpt        + More
IPR020683   Ankyrin_rpt-contain_dom       
IPR036770   Ankyrin_rpt-contain_sf       
IPR002893   Znf_MYND       
IPR001214   SET_dom       
IPR038035   EHMT1       
IPR007728   Pre-SET_dom       
IPR013083   Znf_RING/FYVE/PHD       
IPR003613   Ubox_domain       
IPR036390   WH_DNA-bd_sf       
IPR036388   WH-like_DNA-bd_sf       
IPR001995   Peptidase_A2_cat       
IPR031352   SesA       
IPR027417   P-loop_NTPase       

SUPFAM

SSF48403   SSF48403        + More
SSF46785   SSF46785       
SSF52540   SSF52540       

Gene 3D

1.25.40.20     3.30.40.10     1.10.10.10    

CDD

cd00204  ANK

ProteinModelPortal

H9JJ70   A0A194RW00   A0A194Q060   A0A154NY84   A0A090MA93   A0A087V491   + More
B3EU24   C3NIT9   X1H8V7   A0A1F9AH59   A0A2P6KIZ5   A0A0P7V8U0   A0A3M0IXE2   U3JSN1   I4BZW7   A0A0B8N264   A0A091SQI4   A0A1W4YJ43   A0A225V0M0   H0YXA8   A0A1S3GA49   W5M0N2   A0A218UDV4   A0A1S3G7V3   A0A1S3G885   A0A2S7Q6R5   A0A093SJC5   U3IXU8   A0A3B0YXP3   G2Y6T1   A0A384J8K7   M7TUB6   A0A091IZR3   A0A091QV00   A0A3Q0H4H9   A0A1V4J783   A0A287DCB6   R0L5Y1   A0A3Q0H944   I3LWJ6   A0A091CQK6   A0A1V4J7B8   A0A2K6GI19   Q47DZ9   A0A2J8RMH6   Q4EC44   L8HI15   Q8BHB2   A0A1V3FVR2   A0A2J8PWU5   A0A1B0GWF6   F6UJH7   A0A1D5PVY2   A0A3Q0H4J8   G3UQS6   G3WHW7   A0A3Q0H949   A0A3Q0H964   A0A3L7HD24   A0A3Q0H4I9   A0A1D5PW08   G5BG04   C0R391   Z4YJZ7   A0A1D5PJ82   A0A151MF60   Q5DW34-2   A0A1U7SD70   A0A0H2UH19   A0A250Y7B8   A0A151MF43   Q5DW34   K7FDM7   G3I5Z2   A0A151MFI4   A0A0P6J5K3   A0A3L8SDY2   H9GIS7   K7FDN4   Q73I01   A0A3Q2U3H4   A0A226PMU1   A0A1D5PIX7   A0A3Q0H5S0   A0A151MF76   A0A0Q3MA63   E9Q5A3   H2R5Y8   D4A005   A0A226NG00   A0A1A6GSP9   H0V8M4   H7CHP8   G1RDJ8   Q5DW34-3   G1MU25   A0A286XW35   A0A1D5P7T6   Q5F3H1   A0A1D5P0G7  

PDB

6BY9     E-value=1.33971e-16,     Score=208

KEGG

101737685    no KO assigned | (RefSeq) histone-lysine N-methyltransferase EHMT1-like

GO

GO:0016301   GO:0046872   GO:0018024   GO:0005694   GO:0005634   GO:0002039   GO:0008270   GO:0004842   GO:0016021   GO:0046976   GO:0018027   GO:0046974   GO:0070742   GO:0000122   GO:0018026   GO:0045995   GO:0006306   GO:0120162   GO:0016604   GO:0004190   GO:0008168   GO:0006325   GO:0016571   GO:0045892   GO:0005654   GO:0060992   GO:0016279   GO:0005515   GO:0016020   GO:0017038   GO:0006605   GO:0006886   GO:0003824   GO:0008152   GO:0016491   GO:0015930  

PANTHER

PTHR46307:SF2  

Subcellular location

Chromosome  
Nucleus  

Length:

230

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00036

Exp number, first 60 AAs:

0.00035

Total prob of N-in:

0.07589

outside

1  -  230

 

 

Pi

330.993714

Theta

216.693499

Tajima's D

1.362947

CLR

0

CSRT

0.750762461876906

Interpretation

Uncertain