Gene

KWMTBOMO14694

Pre Gene Modal

BGIBMGA009620

Annotation

PREDICTED:_LOW_QUALITY_PROTEIN:_E3_ubiquitin-protein_ligase_RFWD2-like_[Plutella_xylostella]

Location

Bomo_Chr24(+):12900654-12905900      View in EpiBrowser

Full name

E3 ubiquitin-protein ligase COP1      

Alternative Name

Constitutive photomorphogenesis protein 1 homolog
RING finger and WD repeat domain protein 2
RING finger protein 200
RING-type E3 ubiquitin transferase RFWD2

Location in the cell

Nuclear   Reliability : 1.065

CDS

ATGATTCAATTAGAAAGTAGCGCGGGCGCGGTGTGCTCGGAGGAGGAGAGCTTCGCGGGCGGCGCGGGGGGCGGGGGGGACGCCCTGGCCGCGCGCTGGAGGAAGCTCACGCAACACTTCGACGACTTCGTGCAGTGTTACTTCGCGCACCGCGCTGACGAGCTGTACTTCCCGGGGTCGGGGTCGCTGACCCCCGCGTCCCCCGCCGTCCCCGCCTACCCCGCACCCCCCACCGTGACCCCCGTGCCCTCCTCGGCGGCCCCGTACACGTGCGAGGCGGACCACGTGCCGCAGGGTCCGGTGTGCGACCCGCAACCAACTCCTGCACCGGGTCCGTCGGACCAGCGGGCGCCGGTGGCGGGCGTGGCGGTCGAGGACTCCGTGGAGATGGAGGAGGCCGGCTCCACTCGAGCCCGCGCCGGACTCGACGCCTTCCGGGACGACCTGGTCGCTTTCACCGCCTACAGGACGCTACGGCCGCTGGCCACGCTGTCGTACTGCAGCGACGCCATCAACTACTCCACGATCGTGTCCACCATCGAGTTCGACAAGGACGAGGAGTTCTTCGCGATCGCCGGCGTCACCAAGAGGATCAAGGTGTTCGAGTTCGAGTCGGTGGTGCGCGACGCGGTGGACGTGCACTACCCGTGCGCAGAGATGCAGTGCTCGCACAAGATCTCGTGCGTGTCGTGGAACAAGTACCACAAGCACGTGCTGGCGTCGTCGGACTACGAGGGCACGGTGTCGGTGTGGGACGCGGGCACGGCGGTGCGCACGCGCGCGCTGCAGGAGCACGACAAGCGCGCCTGGTCCGTGCACTTCAACCGCGCCGACGTGCGCCTGCTGGCCTCGGGCTCCGACGACGCGCGCGTCAAGCTGTGGGCGCTCAACCAGGAGAAGTCCGTCGCCACGCTCGAGGCCAAGTTCAACGTGTGCTGCGTGCGCTTCAACCCCAACTCGTCCTGCCACCTGGCCTTCGGCTCCGCAGACCACTGCGTGCACTACTACGACCTGCGCTCGCCGCGCGCGCCGCTGGCCGTGTTCCGGGACCACCGCAAGGCGGTCTCGTACGTGAAGTTCCTGGACGGCGGCACGCTGGTGTCCGCCTCCACGGACTCGCAGCTCAAGCTGTGGCGCGTGGAGTCCCCGCGCTGCGTGCGCTCCTTCACCGGACACACCAACGAGAAGAACTTTGTGGGGCTGGCCACCGACGGCCGCTACGTGGCGTGCGGCTCGGAGAACAACGCGCTCTACGTGTACCACAGCGGCCTGGCGCGCCCCTGCCTCGCCTACAGGTTCGACCCCGCGCGCCCCCTCCTCGAGCGCGACCGCCGCGACGAGGAGCCCGCAGAGTTCGTCTCCGCCGTCTGCTGGAGGAGGGCGGCCTCCCGCCCCGCCCTCCTCGCCGCCAACAGCCACGGCACCATCAAGGTCCTCGAGCTCGTCTGA

Protein

MIQLESSAGAVCSEEESFAGGAGGGGDALAARWRKLTQHFDDFVQCYFAHRADELYFPGSGSLTPASPAVPAYPAPPTVTPVPSSAAPYTCEADHVPQGPVCDPQPTPAPGPSDQRAPVAGVAVEDSVEMEEAGSTRARAGLDAFRDDLVAFTAYRTLRPLATLSYCSDAINYSTIVSTIEFDKDEEFFAIAGVTKRIKVFEFESVVRDAVDVHYPCAEMQCSHKISCVSWNKYHKHVLASSDYEGTVSVWDAGTAVRTRALQEHDKRAWSVHFNRADVRLLASGSDDARVKLWALNQEKSVATLEAKFNVCCVRFNPNSSCHLAFGSADHCVHYYDLRSPRAPLAVFRDHRKAVSYVKFLDGGTLVSASTDSQLKLWRVESPRCVRSFTGHTNEKNFVGLATDGRYVACGSENNALYVYHSGLARPCLAYRFDPARPLLERDRRDEEPAEFVSAVCWRRAASRPALLAANSHGTIKVLELV

Description

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Involved in JUN ubiquitination and degradation. Directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1. Involved in 14-3-3 protein sigma/SFN ubiquitination and proteasomal degradation, leading to AKT activation and promotion of cell survival. Ubiquitinates MTA1 leading to its proteasomal degradation. Upon binding to TRIB1, ubiquitinates CEBPA, which lacks a canonical COP1-binding motif (Probable).

Catalytic Activity

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.

Subunit

Homodimer. Homodimerization is mediated by the coiled coil domain. Component of the DCX DET1-COP1 ubiquitin ligase complex at least composed of RBX1, DET1, DDB1, CUL4A and COP1. Isoform 2 does not interact with CUL4A but still binds to RBX1, suggesting that the interaction may be mediated by another cullin protein. Isoform 1 and isoform 2 interact with CUL5 but not with CUL1, CUL2 not CUL3. Interacts with bZIP transcription factors JUN, JUNB and JUND but not with FOS, ATF2 nor XBP1. Interacts with p53 (TP53). Interacts with COPS6; this interaction stabilizes RFWD2 through reducing its auto-ubiquitination and decelerating its turnover rate. Interacts with SFN; this interaction leads to SFN degradation. Isoform 4 forms heterodimers with isoform 1, preventing its association with DET1. Interacts with p53/TP53 and MTA1. Interacts with TRIB1 (via C-terminus) and TRIB2 (PubMed:20410507, PubMed:27041596).

Similarity

Belongs to the COP1 family.

Keywords

3D-structure   Alternative splicing   Coiled coil   Complete proteome   Cytoplasm   Metal-binding   Nucleus   Reference proteome   Repeat   Transferase   Ubl conjugation   Ubl conjugation pathway   WD repeat   Zinc   Zinc-finger  

Feature

chain  E3 ubiquitin-protein ligase COP1
splice variant  In isoform 3.

Uniprot

H9JJB9   A0A2H1WAK2   A0A194QU24   A0A212FBZ3   A0A2A4J9J5   A0A2W1B778   + More
A0A1B6L716   A0A1B6CBX0   A0A2P8YKN3   A0A1B6JH28   A0A1B6FDX3   A0A0N7Z8H0   A0A0J7NTT0   A0A195BRB7   A0A2J7PN84   A0A158NZF3   A0A088ADG8   K7J750   A0A0L7RJM9   E2B9F2   A0A232FG18   A0A2A3EDH8   A0A0M8ZZF6   T1I8V9   A0A154P1Z2   A0A0V0G806   D6WAL1   A0A224XJS6   A0A0A9WSN1   A0A310SJF4   A0A2R7X0M9   A0A0T6BAI2   A0A146LYB2   A0A067R1R4   A0A1Z5LHR7   V5HY20   A0A2R5LMI9   A0A293L690   E0VY83   A0A1Y1MGZ3   A0A131ZAM6   A0A224YWI5   A0A224YXC8   A0A131YQH5   L7M554   A0A131XC23   X1XB79   A0A2H8TLK8   A0A2S2PLB0   A0A026WCC7   N6U1H1   A0A1E1XD65   A0A1A8AFQ6   T1JKE0   Q5BJY0   A0A1U8BR60   A0A340WX38   V9KJ35   A0A250Y1X6   A0A340WPI1   A0A2K5PH54   U6D0K2   A0A1S3WE28   A0A3Q0DY25   A0A340WWG4   A0A2U3XGE1   A0A2U3ZDH9   H9F8U6   A0A2U4CRD5   Q8NHY2-3   A0A1V9XYE5   G3HA05   A0A218V488   A0A2I0MC37   A0A2I0MC58   K7FZ14   A0A2J8N6Z8   A0A2J8UC33   H0Y339   A0A3Q0DU09   A0A1S3JBR8   A0A1U7RGS4   A0A091DK72   A0A3Q0DX67   A0A3Q0DQN3   A0A2U3XGG5   A0A3Q0DQN9   X5D9B8   A0A060WTQ7   A0A1S3WET0   A0A3Q2CY50   U3K105   A0A3Q2CY30   A0A2U4CR21  

EC Number

2.3.2.27

Pubmed

19121390    26354079    22118469    28756777    29403074    27129103    + More
21347285    20075255    20798317    28648823    18362917    19820115    25401762    26823975    24845553    28528879    25765539    20566863    28004739    26830274    28797301    25576852    28049606    24508170    23537049    28503490    15489334    24402279    28087693    25319552    12466024    12615916    14739464    24722188    14702039    16710414    15103385    17968316    19837670    19805145    20410507    21625211    27041596    28327890    21804562    23371554    17381049    24755649   

EMBL

BABH01036433    BABH01036434    BABH01036435    ODYU01007377    SOQ50083.1    KQ461108    + More
KPJ08972.1    AGBW02009260    OWR51243.1    NWSH01002382    PCG68426.1    KZ150253    PZC71799.1    GEBQ01020465    JAT19512.1    GEDC01026357    JAS10941.1    PYGN01000528    PSN44774.1    GECU01009202    JAS98504.1    GECZ01021343    JAS48426.1    GDKW01003371    JAI53224.1    LBMM01001743    KMQ95825.1    KQ976423    KYM88754.1    NEVH01023957    PNF17804.1    ADTU01000886    AAZX01008222    KQ414581    KOC71003.1    GL446502    EFN87676.1    NNAY01000297    OXU29379.1    KZ288271    PBC29833.1    KQ435812    KOX72753.1    ACPB03024616    KQ434803    KZC05946.1    GECL01001995    JAP04129.1    KQ971312    EEZ97982.1    GFTR01007584    JAW08842.1    GBHO01033174    GBHO01033173    GDHC01008730    JAG10430.1    JAG10431.1    JAQ09899.1    KQ760549    OAD59875.1    KK856260    PTY25326.1    LJIG01002847    KRT84093.1    GDHC01007003    JAQ11626.1    KK852811    KDR15937.1    GFJQ02000055    JAW06915.1    GANP01003086    JAB81382.1    GGLE01006573    MBY10699.1    GFWV01002787    MAA27517.1    DS235845    EEB18339.1    GEZM01031084    GEZM01031083    JAV85072.1    GEDV01000687    JAP87870.1    GFPF01007507    MAA18653.1    GFPF01007458    MAA18604.1    GEDV01007370    JAP81187.1    GACK01006815    JAA58219.1    GEFH01004936    JAP63645.1    ABLF02034147    GFXV01003218    MBW15023.1    GGMR01017439    MBY30058.1    KK107321    EZA52694.1    APGK01053202    KB741222    KB631665    ENN72402.1    ERL85146.1    GFAC01001996    JAT97192.1    HADY01014585    SBP53070.1    AFFK01019697    BC091284    AAH91284.1    JW865412    AFO97929.1    GFFV01002359    JAV37586.1    HAAF01003812    CCP75637.1    JU327299    AFE71055.1    AF508940    BK000438    AF527539    AY509921    KJ534928    KJ535076    AK025789    AK314750    AL162736    AL359265    AL513329    AL590723    CH471067    BC094728    MNPL01002182    OQR78480.1    JH000242    EGV96157.1    MUZQ01000049    OWK60895.1    AKCR02000021    PKK27247.1    PKK27246.1    AGCU01203946    AGCU01203947    AGCU01203948    AGCU01203949    AGCU01203950    NBAG03000235    PNI67549.1    NDHI03003464    PNJ42804.1    KN122390    KFO30665.1    KJ534933    AHW56573.1    FR904722    CDQ70546.1    AGTO01011210   

Proteomes

UP000005204    UP000053240    UP000007151    UP000218220    UP000245037    UP000036403    + More
UP000078540    UP000235965    UP000005205    UP000005203    UP000002358    UP000053825    UP000008237    UP000215335    UP000242457    UP000053105    UP000015103    UP000076502    UP000007266    UP000027135    UP000009046    UP000007819    UP000053097    UP000019118    UP000030742    UP000189706    UP000265300    UP000233040    UP000079721    UP000189704    UP000245341    UP000245340    UP000245320    UP000005640    UP000192247    UP000001075    UP000197619    UP000053872    UP000007267    UP000085678    UP000189705    UP000028990    UP000193380    UP000265020    UP000016665   

PRIDE

H9JJB9    Q8NHY2    H0Y339   

Pfam

PF00400   WD40        + More
PF00883   Peptidase_M17
PF02789   Peptidase_M17_N

Interpro

IPR036322   WD40_repeat_dom_sf        + More
IPR001841   Znf_RING       
IPR001680   WD40_repeat       
IPR017986   WD40_repeat_dom       
IPR013083   Znf_RING/FYVE/PHD       
IPR017907   Znf_RING_CS       
IPR015943   WD40/YVTN_repeat-like_dom_sf       
IPR020472   G-protein_beta_WD-40_rep       
IPR019775   WD40_repeat_CS       
IPR003613   Ubox_domain       
IPR008283   Peptidase_M17_N       
IPR011356   Leucine_aapep/pepB       
IPR000819   Peptidase_M17_C       

SUPFAM

SSF50978   SSF50978       

Gene 3D

2.130.10.10     3.30.40.10    

CDD

cd00433  Peptidase_M17

ProteinModelPortal

H9JJB9   A0A2H1WAK2   A0A194QU24   A0A212FBZ3   A0A2A4J9J5   A0A2W1B778   + More
A0A1B6L716   A0A1B6CBX0   A0A2P8YKN3   A0A1B6JH28   A0A1B6FDX3   A0A0N7Z8H0   A0A0J7NTT0   A0A195BRB7   A0A2J7PN84   A0A158NZF3   A0A088ADG8   K7J750   A0A0L7RJM9   E2B9F2   A0A232FG18   A0A2A3EDH8   A0A0M8ZZF6   T1I8V9   A0A154P1Z2   A0A0V0G806   D6WAL1   A0A224XJS6   A0A0A9WSN1   A0A310SJF4   A0A2R7X0M9   A0A0T6BAI2   A0A146LYB2   A0A067R1R4   A0A1Z5LHR7   V5HY20   A0A2R5LMI9   A0A293L690   E0VY83   A0A1Y1MGZ3   A0A131ZAM6   A0A224YWI5   A0A224YXC8   A0A131YQH5   L7M554   A0A131XC23   X1XB79   A0A2H8TLK8   A0A2S2PLB0   A0A026WCC7   N6U1H1   A0A1E1XD65   A0A1A8AFQ6   T1JKE0   Q5BJY0   A0A1U8BR60   A0A340WX38   V9KJ35   A0A250Y1X6   A0A340WPI1   A0A2K5PH54   U6D0K2   A0A1S3WE28   A0A3Q0DY25   A0A340WWG4   A0A2U3XGE1   A0A2U3ZDH9   H9F8U6   A0A2U4CRD5   Q8NHY2-3   A0A1V9XYE5   G3HA05   A0A218V488   A0A2I0MC37   A0A2I0MC58   K7FZ14   A0A2J8N6Z8   A0A2J8UC33   H0Y339   A0A3Q0DU09   A0A1S3JBR8   A0A1U7RGS4   A0A091DK72   A0A3Q0DX67   A0A3Q0DQN3   A0A2U3XGG5   A0A3Q0DQN9   X5D9B8   A0A060WTQ7   A0A1S3WET0   A0A3Q2CY50   U3K105   A0A3Q2CY30   A0A2U4CR21  

PDB

5HQG     E-value=8.58299e-119,     Score=1093

KEGG

101746476    K10143 E3 ubiquitin-protein ligase RFWD2 [EC:2.3.2.27] | (RefSeq) E3 ubiquitin-protein ligase RFWD2

PATHWAY

04120    Ubiquitin mediated proteolysis - Bombyx mori (domestic silkworm)

GO

GO:0016874   GO:0004842   GO:0032440   GO:0004177   GO:0008235   GO:0005737   GO:0030145   GO:0000139   GO:0005829   GO:0005654   GO:0046872   GO:0043687   GO:0010212   GO:0043161   GO:0031464   GO:0016607   GO:0032436   GO:0061630   GO:0005515   GO:0008270   GO:0042803   GO:0051087   GO:0005634   GO:0006303   GO:0003824   GO:0008152   GO:0016491   GO:0015930  

Subcellular location

Nucleus speckle  
Cytoplasm  

Length:

482

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00133

Exp number, first 60 AAs:

0.00011

Total prob of N-in:

0.00121

outside

1  -  482

 

 

Pi

193.351058

Theta

135.554876

Tajima's D

1.473201

CLR

0

CSRT

0.774061296935153

Interpretation

Uncertain