Gene

KWMTBOMO14632  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA009562

Annotation

proteasome_25_kDa_subunit_[Bombyx_mori]

Location

Bomo_Chr24(+):11599559-11604468      View in EpiBrowser

Full name

Proteasome subunit alpha type       + More
Proteasome endopeptidase complex      
Proteasome subunit alpha type-2      

Alternative Name

PROS-Dm25
Proteasome 25 kDa subunit

Location in the cell

Cytoplasmic   Reliability : 1.102 Mitochondrial   Reliability : 1.642

CDS

ATGGCGTCGGAGAGATACAGTTTTTCTCTCACAACATTTAGCCCCTCGGGCAAATTAGTGCAAATCGAATATGCTTTAGCTGCCGTAGCCGCCGGTGGTACTTCCGTCGGCATCAAAGCGTCAAATGGCGTCGTGATTGCCACAGAGAACAAACACAAGAGCATACTGTATGATGAACACAGTGTCAACAAAGTCGAGATGATAACTGGCCACATTGGCATGGTTTACTCGGGAATGGGTCCAGACTATCGTCTACTGGTGACTCAAGCAAGGAAGATGGCCCAGCAGTACTTCTTGATGTATCATGAGCCGATCCCGACTGCGCAATTAGTGCAACGAGTAGCTACGGTCATGCAGGAGTACACACAGTCGGGTGGCGTCCGACCCTTTGGGGTATCTCTATTGATCTGTGGCTGGGAAGAAGGACGTCCTTACCTGTTCCAGTGTGACCCCTCCGGAGCCTATTTTGCATGGAAAGCCACAGCCATGGGAAAGAACTTTAATAATGGAAAAACTTTCCTTGAAAAAAGATATACAGAAGACTTGGAGTTAGATGACGCAGTTCACACAGCTATCTTGACCCTTAAGGAAGGTTTTGAGGGTCAAATGACTGCAGATAACATAGAAGTTGGCATCTGTGACGCGAGCGGGTTCCGTCGCTTAGAGCCAGCCCACGTGAAGGATTATCTGGCTAACATACCTTAA

Protein

MASERYSFSLTTFSPSGKLVQIEYALAAVAAGGTSVGIKASNGVVIATENKHKSILYDEHSVNKVEMITGHIGMVYSGMGPDYRLLVTQARKMAQQYFLMYHEPIPTAQLVQRVATVMQEYTQSGGVRPFGVSLLICGWEEGRPYLFQCDPSGAYFAWKATAMGKNFNNGKTFLEKRYTEDLELDDAVHTAILTLKEGFEGQMTADNIEVGICDASGFRRLEPAHVKDYLANIP

Description

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.

Catalytic Activity

Cleavage of peptide bonds with very broad specificity.

Subunit

The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits.
The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel (By similarity). Interacts with Rpn6.

Similarity

Belongs to the peptidase T1A family.

Keywords

Complete proteome   Cytoplasm   Hydrolase   Nucleus   Protease   Proteasome   Reference proteome   Threonine protease  

Feature

chain  Proteasome subunit alpha type-2

Uniprot

Q1HQB7   A0A212F9V0   A0A194QA20   A0A2W1BFN6   A0A2A4JWQ7   A0A2H1VM92   + More
I4DN88   A0A3S2NTZ1   A0A1E1WV72   S4NSC8   A0A0C5APJ2   A0A1Q3FY69   A0A1B0DFF8   A0A1B0GHK9   A0A1L8DSM6   U4UX56   Q177R1   U5EX71   A0A023EKK1   A0A0K8TSL4   A0A2J7Q4L3   D6WBM8   V5GRW0   A0A232FG31   K7IPF2   A0A067QTL1   R4UVS9   A0A1I8NAS9   A0A1W4WV84   A0A0L0CHE0   A0A1L8EHB4   A0A026WEK7   A0A1Y1KUA0   A0A0J7L0C6   E2BNG0   E2AM64   A0A1I8QF75   A0A151IKY8   A0A151IVP7   F4X381   A0A195BDN3   A0A158NJZ6   A0A151WEI3   A0A151K3J4   A0A084VHH7   A0A182J0F1   A0A182XVV1   A0A182LU26   A0A182PKJ1   A0A182T728   A0A182Q9J8   A0A182V8A0   A0A182NS72   A0A182JQP0   A0A182U3D1   A0A182VYZ8   A0A182KLS8   A0A182XBC9   Q8T0Y8   A0A182RPU2   A0A182I071   A0A1B0GEQ9   A0A1A9XM43   A0A1A9VDH1   A0A1B0BQC2   A0A1A9Z865   A0A336M7H7   A0A2M3ZHP9   A0A2M4AG69   W5JL01   A0A182F6U2   A0A088AQ82   A0A1A9WPT6   E0VPK7   A0A1B6II94   T1E9P1   A0A1B6KSD2   A0A2A3E8Z1   B3M2R0   A0A1B6D4E8   A0A3B0JYB6   Q29B24   B4GZ65   A0A1W4UBA2   A0A2J7Q4L5   B4PP99   B3P4B8   B4K880   B4QSA9   B4HGX8   P40301   B4LYA3   B4JTP1   A0A0P5V4U2   A0A0P5B1E3   A0A0P5DQQ1   A0A0P5B1Q5   A0A0N8ASF0   A0A0P5HQ40   A0A0P5I6P2  

EC Number

3.4.25.1

Pubmed

19121390    22118469    26354079    28756777    22651552    23622113    + More
25653432    23537049    17510324    24945155    26483478    26369729    18362917    19820115    28648823    20075255    24845553    25315136    26108605    24508170    30249741    28004739    20798317    21719571    21347285    24438588    25244985    20966253    12364791    12733668    14747013    17210077    20920257    23761445    20566863    17994087    15632085    17550304    8357840    10731132    12537572    12537569    22187461   

EMBL

BABH01040473    DQ443135    ABF51224.1    AGBW02009557    OWR50511.1    KQ459249    + More
KPJ02269.1    KZ150587    PZC70483.1    NWSH01000465    PCG76216.1    ODYU01003325    SOQ41928.1    AK402776    BAM19378.1    RSAL01000199    RVE44512.1    GDQN01000176    JAT90878.1    GAIX01014147    JAA78413.1    KM999970    AJK90585.1    GFDL01002569    JAV32476.1    AJVK01058724    AJWK01005308    GFDF01004635    JAV09449.1    KB632400    ERL94906.1    CH477372    EAT42402.1    GANO01001093    JAB58778.1    JXUM01120764    GAPW01003926    KQ566429    JAC09672.1    KXJ70142.1    GDAI01000495    JAI17108.1    NEVH01018378    PNF23523.1    KQ971311    EEZ99004.1    GALX01005513    JAB62953.1    NNAY01000286    OXU29480.1    KK853498    KDR07186.1    KC571852    AGM32351.1    JRES01000378    KNC31833.1    GFDG01000800    JAV17999.1    KK107250    QOIP01000002    EZA54383.1    RLU25341.1    GEZM01075290    JAV64158.1    LBMM01001639    KMQ95959.1    GL449414    EFN82739.1    GL440715    EFN65470.1    KQ977151    KYN05276.1    KQ980895    KYN11768.1    GL888609    EGI59062.1    KQ976509    KYM82668.1    ADTU01000290    KQ983238    KYQ46264.1    KQ981606    LKEZ01003746    KYN39594.1    KYN50714.1    ATLV01013171    KE524842    KFB37421.1    AXCP01004750    AXCM01008446    AXCN02000267    AJ416109    AAAB01008987    CAC94781.1    EAA01264.2    APCN01002050    CCAG010012556    JXJN01018551    UFQS01000653    UFQT01000653    SSX05806.1    SSX26165.1    GGFM01007333    MBW28084.1    GGFK01006391    MBW39712.1    ADMH02001255    ETN63429.1    DS235366    EEB15313.1    GECU01035835    GECU01026257    GECU01021069    JAS71871.1    JAS81449.1    JAS86637.1    GAMD01001162    JAB00429.1    GEBQ01025639    JAT14338.1    KZ288320    PBC28253.1    CH902617    EDV42381.1    GEDC01016727    JAS20571.1    OUUW01000005    SPP80500.1    CM000070    EAL27175.1    CH479198    EDW28083.1    PNF23524.1    CM000160    EDW97105.1    CH954181    EDV49363.1    CH933806    EDW15434.1    CM000364    EDX13205.1    CH480815    EDW42449.1    X70304    AE014297    AY069280    CH940650    EDW67991.1    CH916374    EDV91470.1    GDIP01118812    GDIP01106963    GDIP01092860    LRGB01001253    JAL96751.1    KZS13175.1    GDIP01191102    JAJ32300.1    GDIP01152788    JAJ70614.1    GDIP01191101    JAJ32301.1    GDIQ01247459    JAK04266.1    GDIQ01247458    JAK04267.1    GDIQ01217170    JAK34555.1   

Proteomes

UP000005204    UP000007151    UP000053268    UP000218220    UP000283053    UP000092462    + More
UP000092461    UP000030742    UP000008820    UP000069940    UP000249989    UP000235965    UP000007266    UP000215335    UP000002358    UP000027135    UP000095301    UP000192223    UP000037069    UP000053097    UP000279307    UP000036403    UP000008237    UP000000311    UP000095300    UP000078542    UP000078492    UP000007755    UP000078540    UP000005205    UP000075809    UP000078541    UP000030765    UP000075880    UP000076408    UP000075883    UP000075885    UP000075901    UP000075886    UP000075903    UP000075884    UP000075881    UP000075902    UP000075920    UP000075882    UP000076407    UP000007062    UP000075900    UP000075840    UP000092444    UP000092443    UP000078200    UP000092460    UP000092445    UP000000673    UP000069272    UP000005203    UP000091820    UP000009046    UP000242457    UP000007801    UP000268350    UP000001819    UP000008744    UP000192221    UP000002282    UP000008711    UP000009192    UP000000304    UP000001292    UP000000803    UP000008792    UP000001070    UP000076858   

PRIDE

Q1HQB7    A0A088AQ82    P40301   

Pfam

PF00227   Proteasome        + More
PF10584   Proteasome_A_N

Interpro

IPR023332   Proteasome_alpha-type        + More
IPR034644   Proteasome_subunit_alpha2       
IPR001353   Proteasome_sua/b       
IPR029055   Ntn_hydrolases_N       
IPR000426   Proteasome_asu_N       

SUPFAM

SSF56235   SSF56235       

Gene 3D

3.60.20.10    

ProteinModelPortal

Q1HQB7   A0A212F9V0   A0A194QA20   A0A2W1BFN6   A0A2A4JWQ7   A0A2H1VM92   + More
I4DN88   A0A3S2NTZ1   A0A1E1WV72   S4NSC8   A0A0C5APJ2   A0A1Q3FY69   A0A1B0DFF8   A0A1B0GHK9   A0A1L8DSM6   U4UX56   Q177R1   U5EX71   A0A023EKK1   A0A0K8TSL4   A0A2J7Q4L3   D6WBM8   V5GRW0   A0A232FG31   K7IPF2   A0A067QTL1   R4UVS9   A0A1I8NAS9   A0A1W4WV84   A0A0L0CHE0   A0A1L8EHB4   A0A026WEK7   A0A1Y1KUA0   A0A0J7L0C6   E2BNG0   E2AM64   A0A1I8QF75   A0A151IKY8   A0A151IVP7   F4X381   A0A195BDN3   A0A158NJZ6   A0A151WEI3   A0A151K3J4   A0A084VHH7   A0A182J0F1   A0A182XVV1   A0A182LU26   A0A182PKJ1   A0A182T728   A0A182Q9J8   A0A182V8A0   A0A182NS72   A0A182JQP0   A0A182U3D1   A0A182VYZ8   A0A182KLS8   A0A182XBC9   Q8T0Y8   A0A182RPU2   A0A182I071   A0A1B0GEQ9   A0A1A9XM43   A0A1A9VDH1   A0A1B0BQC2   A0A1A9Z865   A0A336M7H7   A0A2M3ZHP9   A0A2M4AG69   W5JL01   A0A182F6U2   A0A088AQ82   A0A1A9WPT6   E0VPK7   A0A1B6II94   T1E9P1   A0A1B6KSD2   A0A2A3E8Z1   B3M2R0   A0A1B6D4E8   A0A3B0JYB6   Q29B24   B4GZ65   A0A1W4UBA2   A0A2J7Q4L5   B4PP99   B3P4B8   B4K880   B4QSA9   B4HGX8   P40301   B4LYA3   B4JTP1   A0A0P5V4U2   A0A0P5B1E3   A0A0P5DQQ1   A0A0P5B1Q5   A0A0N8ASF0   A0A0P5HQ40   A0A0P5I6P2  

PDB

6AVO     E-value=4.36177e-111,     Score=1023

KEGG

732876    K02726 20S proteasome subunit alpha 2 [EC:3.4.25.1] | (RefSeq) proteasome 25 kDa subunit

PATHWAY

03050    Proteasome - Bombyx mori (domestic silkworm)

GO

GO:0004298   GO:0005737   GO:0005634   GO:0019773   GO:0006511   GO:0004175   GO:0010498   GO:0043161   GO:0005839   GO:0010499   GO:0000502   GO:0005829   GO:0008063   GO:0005654   GO:0007623   GO:0051603   GO:0003824   GO:0008152   GO:0016491   GO:0015930  

PANTHER

PTHR11599:SF16  

Subcellular location

Cytoplasm  
Nucleus  

Length:

234

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

1.42552

Exp number, first 60 AAs:

1.23043

Total prob of N-in:

0.03827

outside

1  -  234

 

 

Pi

227.602846

Theta

166.475632

Tajima's D

1.918197

CLR

1.925987

CSRT

0.871656417179141

Interpretation

Uncertain