Gene

KWMTBOMO14536  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA014294

Annotation

PREDICTED:_ATP-citrate_synthase_[Amyelois_transitella]

Location

Bomo_Chr24(-):9830271-9890878      View in EpiBrowser

Full name

ATP-citrate synthase      

Alternative Name

ATP-citrate (pro-S-)-lyase
Citrate cleavage enzyme

Location in the cell

Cytoplasmic   Reliability : 2.071

CDS

ATGTCGTCGAAAGCTATCTACGAGGCAACGGGAAAGGATCTGATCAACAGACACATAGCCTCCGGTACGGCTCTGGTACCGTGCAGGTTCGCCACATTCGAACAGAGCACAGTGTGGGAGGATGAGCTGGGAAAAAATCCATGGCTCTCGAAGGAGCAATTGGTCGTGAAGCCGGACCAGCTAATCAAGCGTCGCGGCAAACTGGGTCTGGTCGGCGTGAACAAGACTGCGGCGGAGGTCCGGCGTTGGCTCGGCGAGCACATGGCCAAGGAGCAGACGATAGGGGCCTGCGTCGGGCGGCTGAGGCACTTTATAGTCGAGCCCTTCGTCAAACACGAACCGAGCGACGAGATGTACCTGTGCATACAGTCGGGCCGGCGCTCCGACACCATCCTGTTCCACCACCAGGGAGGGGTCGACGTCGGAGATGTGGACGCCCTGGCCATCCGACTAGAGGTGCCCGTGGACACCTTCCCGACGGGAGAAGAAGTCGAGAGGGTTTTGTTGAAGAACGTCAAATCTCCATCTACTAAAAGGACCCTGACGACGTTCATAGTGTCTCTATACCGCGTCTACATTGACTTGTACTTCACGTACATGGAGATCAATCCGCTGGTGGTGACCAACGAGAGGATATACCTGCTGGACCTGGCCGCGAAACTGGACCAGACCGCGGACTTCATCTGCGCGAAGAACTGGGGTGAGGTCACGTTCCCTCCGCCCTTCGGGAGGGACGCCTACCCTGAAGAAGCGCACATCGCCGACCTGGACGCCAAGAGCGGGGCCAGTCTGAAGTTGACGGTGCTGAACAAGTCTGGTCGCGTGTGGACGATGGTGGCGGGCGGCGGCGCCAGCGTGGTGTACACGGACACGGTGTGCGCGCTGGGCGGCGCTCACGACCTGGCCAACTACGGGGAGTACTCGGGCGCGCCCACGGAGACGCAGACCGCGGACTACGCGAAGACCATCTTCAGCCTCATGTGCCGCGAGAAGCATCCCGACGGGAAGGTACTGATCGTGGGTGGCGGCATCGCCAACTTCACCAACGTGGCGGATACCTTCCGGGGCATCATCACCGCCATAGAGACGTACAAGGACGCCCTAAAGCAGTACAACGTCACCATCTTCGTGAGGAGAGGGGGACCAAACTACCAGGAGGGACTCAGACAAATGCGCGAAGTGGGCCAGCGCCTCCGCATCCCGCTGTACGTGTTCGGTCCGGAGACGAACATGACGTCCATAGTGGGCCTGGCGCTGGGACACTCGCCCATACCCAAGGAGCACCAGCTCGACTACGCGCCCAAGCAGCTGCCCAGGCCGCAGCCAGCGCCGGCTCCGAAACTGGACCTGCCTGACGTGACCCACAGCCTGCTCGACCTGATCTGCTCCCAGGCTCCGACCAAGCTCGACCTGGGCCAGGGCGTCTCCACCTCGATGCCCCTCTTCAGCGATAGGACGAAGGCCATCATATGGGGCATGCAGAATAGGGCCATACAGGGCATGTTGGACTTCGACTACATCTGCCGGCGCTCGGAGCCCTCGGTCGTCGCCATCGTGTACCCGTTCACGGCGGACCACAAACAGAAGTACTACTTCGGGAACAAGGAAGTCTTCATACCGGTGTTCTCGGACATGGACGTGGCGATGCGCAAGCACCAGGAGGCGACGGTACTCGTCAACTTCGCTTCGCTTCGCTCGGCCTACGACAGCACCATGCAGGCCATGCAGCACCCTCAGATCAACACCGTGGTCATCATCGCGGAGGGCATCCCCGAGAACATGACGCGGAAGATCATCAAGCTCGCTGACGTCAGAGGGGTGAACATAATCGGGCCTGCCACTGTGGGAGGCATTAAACCCGGCTGCTTCAAGATCGGTAACACGGCTGGCATGATTGACAATATCATTGACAGCAAACTGTACCGGCCTGGGAGCGTGGCGTACGTGTCCCGCTCGGGAGGCATGAGCAACGAGCTGAACAAGATCCTGTCGAAGGACGCGGACGGCGTGTGCGAGGGGGTCGCCATCGGGGGCGACCGCTACCCTGGGACCACCTTCATCGACCACCTGCTCAGGTTCGAAGCGGACCCGCAAGTGAAGATGCTGGTGCTGCTTGGAGAAGTGGGGGGAGTCGAGGAGTACCACGTCTGCAAGGCCATCAGGGACGGAATCATCAAGAAGCCCCTCGTGGCCTGGTGCATAGGAACTTGCTCCGACATGTTCACGTCTGAGGTCCAGTTCGGACACGCGGGCTCGCTGGCTGGCTCCGCCGTTGAGAAGGCTGCGGTCAAGAATGAGGCTCTGAAGGCCTACGGAGCGACCGTGCCGGACTCCTTCGACGGCCTAGGGGCTGCCGTGGGCGAGGTCTACCGCAGGCTGGTCGACGAGGGCAAGATCATCGTCAAGGAGGAGGTGGAACCGCCCAAGGTCCCCATGGACTACGATTGGGCTCGGAAGTTGGGTATAATCCGGAAGCCGGCCGCCTTCATCAGCACGATATGCGACGAGCGAGGCCAGGAGCTGAGCTACTGCGGGGTGCCCATCTCCCAGGTGCTGGAGAGGCAGCTCGGGGTCGGCGGCACCGTCAGCCTGCTGTGGTTCCAGCGCGAGCTGCCGGAGTGGGCGAGCCGGTTCTTCGAGCTGGTGCTGATAGTGACCGCGGACCACGGGCCCGCCGTGTCGGGCGCGCACAACACCATGGTCACGGCGCGCGCCGGCAAGGACCTCATCTCCTCCGTCGTCAGCGGCCTGCTCACCATCGGCGACCGGTTCGGTGGGGCCCTGGACCGCGCCGCGGCTGACTTCTGCGCCGCCTACGACAAGGGACAGCACCCCCAGGAGTTCGTCAACGAGAAGAGGGCCAAGGGGGAGCTGATCATGGGCATCGGACACAGGGTGAAGTCGATAAACAACCCGGACTCCCGCGTGCGCGAGCTGAAGGCGTACGTGACGGCGCGCTGGCCCGCGTGGCCGGTCACCCGGTACGCGCTCGACGTGGAGGCCATCACCACCAGGAAGAAGCCGAACCTCATCCTCAACGTGGACGGCATCGTGGCCGCTGCCATGGTCGACCTGTTCCGCCACTGCCAGCTCTTCACGCAAGATGAAGGCAATAATTATATTCAGATGGGCTCAATCAACGCTCTGTTCGTCCTGGGTCGCACGATAGGTCTGGTCGGACATTACTTGGACCAGAAGAGGATGAAGCAGCCTCTGTACCGTCATCCGTGGGACGACATCACCTACATGTCGCCTCTGAACTAA

Protein

MSSKAIYEATGKDLINRHIASGTALVPCRFATFEQSTVWEDELGKNPWLSKEQLVVKPDQLIKRRGKLGLVGVNKTAAEVRRWLGEHMAKEQTIGACVGRLRHFIVEPFVKHEPSDEMYLCIQSGRRSDTILFHHQGGVDVGDVDALAIRLEVPVDTFPTGEEVERVLLKNVKSPSTKRTLTTFIVSLYRVYIDLYFTYMEINPLVVTNERIYLLDLAAKLDQTADFICAKNWGEVTFPPPFGRDAYPEEAHIADLDAKSGASLKLTVLNKSGRVWTMVAGGGASVVYTDTVCALGGAHDLANYGEYSGAPTETQTADYAKTIFSLMCREKHPDGKVLIVGGGIANFTNVADTFRGIITAIETYKDALKQYNVTIFVRRGGPNYQEGLRQMREVGQRLRIPLYVFGPETNMTSIVGLALGHSPIPKEHQLDYAPKQLPRPQPAPAPKLDLPDVTHSLLDLICSQAPTKLDLGQGVSTSMPLFSDRTKAIIWGMQNRAIQGMLDFDYICRRSEPSVVAIVYPFTADHKQKYYFGNKEVFIPVFSDMDVAMRKHQEATVLVNFASLRSAYDSTMQAMQHPQINTVVIIAEGIPENMTRKIIKLADVRGVNIIGPATVGGIKPGCFKIGNTAGMIDNIIDSKLYRPGSVAYVSRSGGMSNELNKILSKDADGVCEGVAIGGDRYPGTTFIDHLLRFEADPQVKMLVLLGEVGGVEEYHVCKAIRDGIIKKPLVAWCIGTCSDMFTSEVQFGHAGSLAGSAVEKAAVKNEALKAYGATVPDSFDGLGAAVGEVYRRLVDEGKIIVKEEVEPPKVPMDYDWARKLGIIRKPAAFISTICDERGQELSYCGVPISQVLERQLGVGGTVSLLWFQRELPEWASRFFELVLIVTADHGPAVSGAHNTMVTARAGKDLISSVVSGLLTIGDRFGGALDRAAADFCAAYDKGQHPQEFVNEKRAKGELIMGIGHRVKSINNPDSRVRELKAYVTARWPAWPVTRYALDVEAITTRKKPNLILNVDGIVAAAMVDLFRHCQLFTQDEGNNYIQMGSINALFVLGRTIGLVGHYLDQKRMKQPLYRHPWDDITYMSPLN

Description

ATP-citrate synthase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues.

Catalytic Activity

acetyl-CoA + ADP + oxaloacetate + phosphate = ATP + citrate + CoA

Subunit

Homotetramer.

Similarity

In the C-terminal section; belongs to the succinate/malate CoA ligase alpha subunit family.
In the N-terminal section; belongs to the succinate/malate CoA ligase beta subunit family.

Uniprot

S4PEX5   A0A0N0P9L9   A0A194R999   A0A1Y1LMG7   A0A1W4WDM4   A0A1W4WEX6   + More
V5GTA5   D2A5W6   A0A182U8G4   A0A182HQV2   A0A2J7RDY5   W8AYI0   A0A1A9XP97   A0A182VKF6   A0A182XLF6   A0A2J7RDZ4   A0A0A1X3Z2   A0A067QJK7   A0A1B0CWI3   A0A0V0G5Z5   A0A182PCH9   A0A224XIL5   A0A182R7Q4   A0A182WA54   A0A034VWU5   A0A1L8E1A7   A0A182Q238   U5EZ83   A0A2M3ZZ32   A0A1A9W2F7   A0A0J9RDY6   A0A1L8E112   A0A2M3ZZ25   A0A0K8U6N3   B4P6T1   A0A0L0CF01   W5J1A9   A0A2M3YY31   A0A182NPW4   A0A0B4LFH8   A0A1W4UZZ4   A0A1L8E0T3   A0A182JI57   A0A182YIL9   B4QHD4   A0A0M4EUF0   A0A182K9C8   B4HSH9   A0A182MUM6   A0A0R1DSP7   A0A069DXN3   A0A2M4BBA3   E2QCF1   A0A1A9ZGT9   A0A3B0IYZ6   A0A1W4VC90   A0A182FMZ3   A0A0J9U372   A0A1W4V0D2   A0A0P4W2W1   N6T8J1   A0A0Q5W116   Q7KN85   A0A0R1DT27   X1WV43   A0A2H8TW78   K7IWV9   B4J9W4   A0A0P8Y505   B3MC04   A0A232FD21   B4KQQ6   Q6AWP8   B3NPZ2   A0A0Q9XH45   A0A0N8P0B2   A0A0G3YKZ0   A0A336M202   A0A1I8P547   Q28WR6   A0A1I8P4X7   A0A0A9WBE8   A0A1I8P4Z2   A0A0R3NQ61   A0A0R3NVZ8   A0A0A9W2Z2   A0A0Q9XLD9   T1P9Z0   A0A0Q5VM32   A0A0Q9W5R4   B4LL37   T1HR11   B4MIX5   A0A336K4N2   B0W198   T1PC34   A0A1I8MVD7   A0A1Q3FL08   A0A1Q3FLA1   A0A026W0M5  

EC Number

2.3.3.8

Pubmed

23622113    26354079    28004739    18362917    19820115    24495485    + More
25830018    24845553    25348373    22936249    17994087    17550304    26108605    20920257    23761445    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    25244985    26334808    27129103    23537049    26109357    26109356    20075255    28648823    18057021    25702953    15632085    25401762    26823975    23185243    25315136    24508170    30249741   

EMBL

GAIX01004302    JAA88258.1    KQ459348    KPJ01339.1    KQ460500    KPJ14207.1    + More
GEZM01055134    JAV73115.1    GALX01005028    JAB63438.1    KQ971345    EFA05014.1    APCN01001603    NEVH01005280    PNF39044.1    GAMC01016623    GAMC01016622    JAB89933.1    PNF39050.1    GBXI01008228    GBXI01002581    JAD06064.1    JAD11711.1    KK853408    KDR07798.1    AJWK01032481    AJWK01032482    GECL01002654    JAP03470.1    GFTR01008545    JAW07881.1    GAKP01011181    GAKP01011180    JAC47771.1    GFDF01001732    JAV12352.1    AXCN02001998    AXCN02001999    GANO01001258    JAB58613.1    GGFK01000438    MBW33759.1    CM002911    KMY94136.1    GFDF01001730    JAV12354.1    GGFK01000440    MBW33761.1    GDHF01033454    GDHF01030000    JAI18860.1    JAI22314.1    CM000158    EDW92008.1    JRES01000487    KNC30821.1    ADMH02002186    ETN57927.1    GGFM01000410    MBW21161.1    AE013599    AHN56267.1    GFDF01001731    JAV12353.1    CM000362    EDX07279.1    KMY94133.1    CP012524    ALC41312.1    CH480816    EDW48057.1    AXCM01000963    KRK00246.1    GBGD01000239    JAC88650.1    GGFJ01001178    MBW50319.1    AAM70940.2    OUUW01000001    SPP73275.1    KMY94135.1    GDKW01000756    JAI55839.1    APGK01047658    APGK01047659    APGK01047660    APGK01047661    KB741084    ENN74028.1    CH954179    KQS62488.1    AF132166    AAD34754.2    AAF58082.2    KRK00245.1    ABLF02035281    ABLF02035282    ABLF02035291    GFXV01005613    MBW17418.1    AAZX01008339    CH916367    EDW02551.1    CH902619    KPU76635.1    EDV37191.1    NNAY01000459    OXU28247.1    CH933808    EDW09255.1    BT015200    AAT94429.1    EDV55839.1    KRG04548.1    KPU76636.1    KM507097    AKM28421.1    UFQS01000101    UFQT01000101    SSW99618.1    SSX19998.1    CM000071    EAL26601.2    GBHO01041446    GBHO01041444    GBRD01005939    GBRD01005938    GDHC01002781    JAG02158.1    JAG02160.1    JAG59882.1    JAQ15848.1    KRT03207.1    KRT03205.1    GBHO01041445    JAG02159.1    KRG04547.1    KA645494    AFP60123.1    KQS62487.1    CH940648    KRF80280.1    EDW61844.1    ACPB03019200    CH963719    EDW72064.1    SSW99619.1    SSX19999.1    DS231821    EDS44968.1    KA645493    AFP60122.1    GFDL01006807    JAV28238.1    GFDL01006789    JAV28256.1    KK107597    QOIP01000013    EZA48609.1    RLU15385.1   

Proteomes

UP000053268    UP000053240    UP000192223    UP000007266    UP000075902    UP000075840    + More
UP000235965    UP000092443    UP000075903    UP000076407    UP000027135    UP000092461    UP000075885    UP000075900    UP000075920    UP000075886    UP000091820    UP000002282    UP000037069    UP000000673    UP000075884    UP000000803    UP000192221    UP000075880    UP000076408    UP000000304    UP000092553    UP000075881    UP000001292    UP000075883    UP000092445    UP000268350    UP000069272    UP000019118    UP000008711    UP000007819    UP000002358    UP000001070    UP000007801    UP000215335    UP000009192    UP000095300    UP000001819    UP000008792    UP000015103    UP000007798    UP000002320    UP000095301    UP000053097    UP000279307   

PRIDE

E2QCF1    Q7KN85    Q6AWP8   

Pfam

PF00285   Citrate_synt        + More
PF00549   Ligase_CoA
PF16114   Citrate_bind
PF02629   CoA_binding
PF08442   ATP-grasp_2

Interpro

IPR033847   Citrt_syn/SCS-alpha_CS        + More
IPR003781   CoA-bd       
IPR013815   ATP_grasp_subdomain_1       
IPR002020   Citrate_synthase       
IPR016143   Citrate_synth-like_sm_a-sub       
IPR014608   ATP-citrate_synthase       
IPR017866   Succ-CoA_synthase_bsu_CS       
IPR032263   Citrate-bd       
IPR005811   CoA_ligase       
IPR016102   Succinyl-CoA_synth-like       
IPR036291   NAD(P)-bd_dom_sf       
IPR036969   Citrate_synthase_sf       
IPR016142   Citrate_synth-like_lrg_a-sub       
IPR013650   ATP-grasp_succ-CoA_synth-type       
IPR017440   Cit_synth/succinyl-CoA_lig_AS       

SUPFAM

SSF48256   SSF48256        + More
SSF51735   SSF51735       
SSF52210   SSF52210       

Gene 3D

1.10.230.10     3.30.1490.20     3.40.50.261     1.10.580.10    

ProteinModelPortal

S4PEX5   A0A0N0P9L9   A0A194R999   A0A1Y1LMG7   A0A1W4WDM4   A0A1W4WEX6   + More
V5GTA5   D2A5W6   A0A182U8G4   A0A182HQV2   A0A2J7RDY5   W8AYI0   A0A1A9XP97   A0A182VKF6   A0A182XLF6   A0A2J7RDZ4   A0A0A1X3Z2   A0A067QJK7   A0A1B0CWI3   A0A0V0G5Z5   A0A182PCH9   A0A224XIL5   A0A182R7Q4   A0A182WA54   A0A034VWU5   A0A1L8E1A7   A0A182Q238   U5EZ83   A0A2M3ZZ32   A0A1A9W2F7   A0A0J9RDY6   A0A1L8E112   A0A2M3ZZ25   A0A0K8U6N3   B4P6T1   A0A0L0CF01   W5J1A9   A0A2M3YY31   A0A182NPW4   A0A0B4LFH8   A0A1W4UZZ4   A0A1L8E0T3   A0A182JI57   A0A182YIL9   B4QHD4   A0A0M4EUF0   A0A182K9C8   B4HSH9   A0A182MUM6   A0A0R1DSP7   A0A069DXN3   A0A2M4BBA3   E2QCF1   A0A1A9ZGT9   A0A3B0IYZ6   A0A1W4VC90   A0A182FMZ3   A0A0J9U372   A0A1W4V0D2   A0A0P4W2W1   N6T8J1   A0A0Q5W116   Q7KN85   A0A0R1DT27   X1WV43   A0A2H8TW78   K7IWV9   B4J9W4   A0A0P8Y505   B3MC04   A0A232FD21   B4KQQ6   Q6AWP8   B3NPZ2   A0A0Q9XH45   A0A0N8P0B2   A0A0G3YKZ0   A0A336M202   A0A1I8P547   Q28WR6   A0A1I8P4X7   A0A0A9WBE8   A0A1I8P4Z2   A0A0R3NQ61   A0A0R3NVZ8   A0A0A9W2Z2   A0A0Q9XLD9   T1P9Z0   A0A0Q5VM32   A0A0Q9W5R4   B4LL37   T1HR11   B4MIX5   A0A336K4N2   B0W198   T1PC34   A0A1I8MVD7   A0A1Q3FL08   A0A1Q3FLA1   A0A026W0M5  

PDB

6O0H     E-value=0,     Score=3531

KEGG

101740166    K01648 ATP citrate (pro-S)-lyase [EC:2.3.3.8] | (RefSeq) ATP-citrate synthase-like

PATHWAY

00020    Citrate cycle (TCA cycle) - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0006629   GO:0005524   GO:0003878   GO:0006101   GO:0048037   GO:0016829   GO:0046872   GO:0005737   GO:0006085   GO:0016021   GO:0005829   GO:0006633   GO:0009346   GO:0016874   GO:0042593   GO:0046912   GO:0003824   GO:0008152   GO:0016491   GO:0015930  

Subcellular location

Cytoplasm  

Length:

1087

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

4.45418

Exp number, first 60 AAs:

0.00199

Total prob of N-in:

0.01841

outside

1  -  1087

 

 

Pi

318.112222

Theta

181.291794

Tajima's D

2.808848

CLR

0.210824

CSRT

0.968201589920504

Interpretation

Uncertain