Gene

KWMTBOMO14501

Pre Gene Modal

BGIBMGA000169

Annotation

Pre-mRNA-splicing_factor_ATP-dependent_RNA_helicase-like_protein_cdc28_[Papilio_xuthus]

Location

Bomo_Chr24(+):8477379-8481890      View in EpiBrowser

Full name

Ceramide phosphoethanolamine synthase      

Location in the cell

PlasmaMembrane   Reliability : 4.816

CDS

ATGCTTGGAAATCGCCTGAAATTTGTTAAACCAGAAGACAAATCTCTCTACCCAATTCGGAACAAAGATGGAGCCATTGAAACACTTATAAATCCGAACACTGATTCAGCACTTGTCATTGATTCTGACTCACACTCTAATTTCGTTTATAATCGCTACCACCACTTACCTTTAGAGAGTCAAAGACAGAAATTACCAATTTTTGAATACAGAAATCATATTTTATATTTGCTGGAGAAGTATCAGACTTTAGTGCTCTGCGGAGAGACTGGATGTGGAAAATCAACTCAAGTGCCACAGTTAACAAACATAATGTGGCCACCATCGCAAGCCAGCAAATTCCTTATTTTGCTTGTACTCATCTTAGTCGTCATTTACTGCGTCTTCATGGACACGCTCTTGTATATGAGAGTGCAGTATTACAGCATAGAAACATTTGAGACTTCAACGCAAACCACATTCCAAGAATCGCCCACAACGAGACTCCGGGCTCTGAGCTATGAAGATGTTGTCTGGATACCTTGCAGTGTGAACCCTTTATGCCATCCAACAGTCAAGGCTTTGATGGTCGATCACATCAACCATTACATCTACGGTCCCTTGTGCGCGATCCTAGATAAAGCATTGAGGATATCCGATAGAATGCTCTTCCTGACACCGAACACAATATCGTGCGCTCACGTTTTTATAGCAATGATTGGCGCGAACCTTTTGACGTGTCCGAATCTAAGCGTCCGTCGTATGGGCATAGTGCTCTTCCAGATCAGGATGTTCCTGGACGATTTGGATGGTCACGTGGCGAGAGAGCGTAAGCATATCAGAGGTGAAAGGTCAGAAGTCGGCAGCCTTGGTTACTGGGTCGACGGAATTTGCGATTTAATTGGCGTAGTCGCGATGATGCTCGGCATATTCGTGTACTTCAAGCAGTATCCGCCCAGACGTGGGTACAGGGGTACTTCCGCATCAGCGTTACCGTACTATCAGCTAAAAGAGATGAACGCTGCCGAGAATATGGAAAAGGATCACGCGTCAGACGTGGGGATCTCGTACAAAACCAAAGTGTCTCTGAAGGCGATAATTCAAGTTATTATACTGTTTTCTGGTCAAATGGTACTGTCTTCTGTCGCCTGGAATAGATATATAAATGTTTACCAGGAAATGTTCGAGAACGGCAATGGATATCTTGCCGGGAGGCTGGTCACTTTTAGATCTGTGAGATTCTTTTGTGCCACTTTGATGTGGAGGATATTGAACCCGCATAGTTACTTGCATGTTCTATCGCTAGCCGTGTTTATTGATAAGACGTGGTCTCTCCTGAAATCGGTCCGATATATCGGCTACGTGCTGTTGTTACTGGCCGTTTTTGTAACTGAATATTTGATTAGTGGCTTCGAAGCCTCCGTTAAAACCTCCGTTGATGGTAGATAA

Protein

MLGNRLKFVKPEDKSLYPIRNKDGAIETLINPNTDSALVIDSDSHSNFVYNRYHHLPLESQRQKLPIFEYRNHILYLLEKYQTLVLCGETGCGKSTQVPQLTNIMWPPSQASKFLILLVLILVVIYCVFMDTLLYMRVQYYSIETFETSTQTTFQESPTTRLRALSYEDVVWIPCSVNPLCHPTVKALMVDHINHYIYGPLCAILDKALRISDRMLFLTPNTISCAHVFIAMIGANLLTCPNLSVRRMGIVLFQIRMFLDDLDGHVARERKHIRGERSEVGSLGYWVDGICDLIGVVAMMLGIFVYFKQYPPRRGYRGTSASALPYYQLKEMNAAENMEKDHASDVGISYKTKVSLKAIIQVIILFSGQMVLSSVAWNRYINVYQEMFENGNGYLAGRLVTFRSVRFFCATLMWRILNPHSYLHVLSLAVFIDKTWSLLKSVRYIGYVLLLLAVFVTEYLISGFEASVKTSVDGR

Description

Catalyzes the biosynthesis of ceramide phosphoethanolamine (CPE) through the transfer of a phosphatidyl head group from cytidine 5'-diphosphate (CDP)-ethanolamine on to the primary hydroxyl of ceramide.

Catalytic Activity

an N-acylsphing-4-enine + CDP-ethanolamine = CMP + H(+) + N-acylsphing-4-enine 1-phosphoethanolamine

Cofactor

Mn(2+)

Similarity

Belongs to the CDP-alcohol phosphatidyltransferase class-I family.
Belongs to the GST superfamily.

Keywords

Cell membrane   Complete proteome   Golgi apparatus   Lipid biosynthesis   Lipid metabolism   Membrane   Phospholipid metabolism   Reference proteome   Sphingolipid metabolism   Transferase   Transmembrane   Transmembrane helix  

Feature

chain  Ceramide phosphoethanolamine synthase

Uniprot

H9ISE2   A0A2H1VZ35   A0A0N1PF37   A0A0N1PGT0   A0A2W1BU13   A0A2A4K2W6   + More
A0A212EK32   A0A182J7H8   A0A182Q1E4   A0A182MIK1   A0A067QU38   B3MCK9   A0A1L8D9N7   A0A0M3QVL4   B4MRQ0   A0A1W4V1E5   B4KMD8   A0A1L8EHY1   B4LNX5   A0A139WLT0   A0A1A9UYD8   A0A1I8NQG2   A0A1A9ZRD3   A0A1B0F9I2   A0A0L0BQA9   A0A1B0DAL5   B4PA83   B3NPT9   A0A0B4LGC8   O77475   T1PG01   B4GCR0   B4QB09   B4I8U7   A0A1A9YJQ6   A0A3B0J2D3   A0A0A1X3R6   Q16H48   A0A1Y1L2D5   E2BPC7   A0A0C9REJ0   A0A151ILY1   A0A1J1J6N9   A0A195EVH6   A0A195E5R1   A0A1B0CKW2   A0A151WWW9   W8CCY4   A0A232EUA0   U4U4I9   A0A1Y1KX30   A0A087ZX33   E9I8P3   A0A0J7K7U5   A0A226E7Y0  

EC Number

2.7.8.n3

Pubmed

19121390    26354079    28756777    22118469    24845553    17994087    + More
18057021    18362917    19820115    26108605    17550304    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    10191082    12537569    23449981    25315136    22936249    25830018    17510324    28004739    20798317    24495485    28648823    23537049    21282665   

EMBL

BABH01024384    ODYU01005330    SOQ46067.1    KQ459286    KPJ02043.1    KQ460501    + More
KPJ14168.1    KZ149963    PZC76270.1    NWSH01000222    PCG78244.1    AGBW02014345    OWR41834.1    AXCN02001123    AXCM01002708    KK852950    KDR13355.1    CH902619    EDV36243.1    GFDF01010898    JAV03186.1    CP012524    ALC42609.1    CH963850    EDW74789.1    CH933808    EDW09826.1    GFDG01000449    JAV18350.1    CH940648    EDW61144.1    KRF79814.1    KRF79815.1    KRF79816.1    KQ971321    KYB28787.1    CCAG010000714    JRES01001535    JRES01000890    KNC22196.1    KNC27523.1    AJVK01029188    CM000158    EDW92409.1    KRK00498.1    CH954179    EDV56880.1    AE013599    AHN56583.1    AHN56584.1    AHN56585.1    AB010261    AB010264    AY060752    AAF47081.1    AAL28300.1    BAA32689.1    KA647686    AFP62315.1    CH479181    EDW31508.1    CM000362    CM002911    EDX08446.1    KMY96139.1    CH480824    EDW57022.1    OUUW01000001    SPP73153.1    GBXI01017320    GBXI01008907    JAC96971.1    JAD05385.1    CH478204    CH477488    EAT33570.1    EAT40097.1    GEZM01066510    GEZM01066509    JAV67842.1    GL449613    EFN82452.1    GBYB01005391    JAG75158.1    KQ977085    KYN05874.1    CVRI01000074    CRL08048.1    KQ981965    KYN31889.1    KQ979608    KYN20503.1    AJWK01016669    AJWK01016670    AJWK01016671    AJWK01016672    AJWK01016673    AJWK01016674    KQ982685    KYQ52338.1    GAMC01002892    GAMC01002891    JAC03665.1    NNAY01002165    OXU21897.1    KB631630    ERL84860.1    GEZM01075817    JAV63946.1    GL761612    EFZ23060.1    LBMM01012230    KMQ86339.1    LNIX01000006    OXA52766.1   

Proteomes

UP000005204    UP000053268    UP000053240    UP000218220    UP000007151    UP000075880    + More
UP000075886    UP000075883    UP000027135    UP000007801    UP000092553    UP000007798    UP000192221    UP000009192    UP000008792    UP000007266    UP000078200    UP000095300    UP000092445    UP000092444    UP000037069    UP000092462    UP000002282    UP000008711    UP000000803    UP000095301    UP000008744    UP000000304    UP000001292    UP000092443    UP000268350    UP000008820    UP000008237    UP000078542    UP000183832    UP000078541    UP000078492    UP000092461    UP000075809    UP000215335    UP000030742    UP000005203    UP000036403    UP000198287   

PRIDE

H9ISE2    O77475   

Pfam

PF01066   CDP-OH_P_transf        + More
PF07717   OB_NTP_bind
PF00271   Helicase_C
PF04408   HA2
PF02798   GST_N
PF14497   GST_C_3

Interpro

IPR000462   CDP-OH_P_trans        + More
IPR027417   P-loop_NTPase       
IPR014001   Helicase_ATP-bd       
IPR002464   DNA/RNA_helicase_DEAH_CS       
IPR000014   PAS       
IPR001650   Helicase_C       
IPR011709   DUF1605       
IPR007502   Helicase-assoc_dom       
IPR036249   Thioredoxin-like_sf       
IPR036282   Glutathione-S-Trfase_C_sf       
IPR004046   GST_C       
IPR004045   Glutathione_S-Trfase_N       
IPR010987   Glutathione-S-Trfase_C-like       
IPR040079   Glutathione_S-Trfase       

SUPFAM

SSF52540   SSF52540        + More
SSF52833   SSF52833       
SSF47616   SSF47616       

CDD

cd00079  HELICc

ProteinModelPortal

H9ISE2   A0A2H1VZ35   A0A0N1PF37   A0A0N1PGT0   A0A2W1BU13   A0A2A4K2W6   + More
A0A212EK32   A0A182J7H8   A0A182Q1E4   A0A182MIK1   A0A067QU38   B3MCK9   A0A1L8D9N7   A0A0M3QVL4   B4MRQ0   A0A1W4V1E5   B4KMD8   A0A1L8EHY1   B4LNX5   A0A139WLT0   A0A1A9UYD8   A0A1I8NQG2   A0A1A9ZRD3   A0A1B0F9I2   A0A0L0BQA9   A0A1B0DAL5   B4PA83   B3NPT9   A0A0B4LGC8   O77475   T1PG01   B4GCR0   B4QB09   B4I8U7   A0A1A9YJQ6   A0A3B0J2D3   A0A0A1X3R6   Q16H48   A0A1Y1L2D5   E2BPC7   A0A0C9REJ0   A0A151ILY1   A0A1J1J6N9   A0A195EVH6   A0A195E5R1   A0A1B0CKW2   A0A151WWW9   W8CCY4   A0A232EUA0   U4U4I9   A0A1Y1KX30   A0A087ZX33   E9I8P3   A0A0J7K7U5   A0A226E7Y0  

PDB

6FAC     E-value=1.93783e-06,     Score=124

KEGG

101737048    no KO assigned | (RefSeq) ceramide phosphoethanolamine synthase

GO

GO:0016780   GO:0016021   GO:0008654   GO:0004386   GO:0005524   GO:0016740   GO:0000139   GO:0006665   GO:0005886   GO:0016020   GO:0043565   GO:0005634   GO:0005507   GO:0004713   GO:0016491   GO:0015930  

Subcellular location

Membrane   The majority of protein localizes to the Golgi apparatus.   With evidence from 3 publications.
Golgi apparatus membrane   The majority of protein localizes to the Golgi apparatus.   With evidence from 3 publications.
Cell membrane   The majority of protein localizes to the Golgi apparatus.   With evidence from 3 publications.

Length:

475

Number of predicted TMHs:

6

Exp number of AAs in TMHs:

122.18883

Exp number, first 60 AAs:

0.00012

Total prob of N-in:

0.37759

outside

1  -  113

TMhelix

114  -  136

inside

137  -  215

TMhelix

216  -  238

outside

239  -  284

TMhelix

285  -  307

inside

308  -  358

TMhelix

359  -  381

outside

382  -  409

TMhelix

410  -  432

inside

433  -  443

TMhelix

444  -  466

outside

467  -  475

 

 

Pi

174.999967

Theta

157.118835

Tajima's D

0.145048

CLR

1.059052

CSRT

0.407929603519824

Interpretation

Uncertain