Gene

KWMTBOMO14470

Pre Gene Modal

BGIBMGA000149

Annotation

PREDICTED:_tubulin-specific_chaperone_E_[Papilio_polytes]

Location

Bomo_Chr24(+):7356049-7371754      View in EpiBrowser

Full name

Lactoylglutathione lyase       + More
Tubulin-specific chaperone E      

Alternative Name

Glyoxalase I
Tubulin-folding cofactor E

Location in the cell

Cytoplasmic   Reliability : 2.365

CDS

ATGGTGACGTTCGGCGTATTTCAAACTTTAGAGTCCCAAGTGGAATTCGGACGCTCGGAAGAGGAGTTGAGGAGAGTCACACATACACTACGCGTTTCCTCGGTAGAGAATATCGCTGGAGACTTGGCTTTCGACATAAGAAAGTATATTGGTGAGGTTCAGGGCTACAAGGGATTATGGTACGGCATAGAATGGGACAACGAGTCCAGAGGCAAGCACGACGGGTGCTTAGATGGCATCCAGTACTTCAAGACTTCCAAACCTAGTGCCGGTTCTTTTGTTCGTCCAAACAAGATCATCCCATCGAAGACTTGCGCGGAAGCCATAAGACAATATTATGGAGATCCGGAGGACGAAACATTAGCTGCTCACCGCAGAACCATCATCAATGAATGGAAGAGGGAAATGGGAGCGCCTTTCATCGAAATGGTTGGTTTTGAAAAAATACACCAAAAACAGAAATTTGATCGCTTACTAGAAGTATGCGTTCACGATCAGAATGTATCAAGAGCTGGTGATGTGTCTTCTCTATGTCCCAACGTGAGAAGCCTCGACGTCTCACGTAACCTCTTCTCCAACTGGCGTGAGGTCATACAACTGTCTGCTCAACTACCGGACTTGAGAGATTTGGATGTTAGCAAGAACCGTATGCTCATCGATGCGCCCACAGAAATACTTGCACAACTTTCAATCAACTTTTCCAAGCTTGAAAAGATCAATCTTTCTGTCTGCGATTATACATGGACGGACATATTGAAATTATCACATTTATGGCCTAAGATACATGAGATAATAGCTGCCTACAATCGTATAGATGAAATCGAACCACCGCTTGTAACTCTGAGGAGTTTATCTGTGCTGAGACTGGATGGAAACCCGATCACCTCTTGGAGTGAAGTGATGAATCTTGGTGCTCTCAATCTGAAGGTTTTGAGTTTGAACGACTGTCTCATTGCGGAGATTAGGTTCGGTGATGATTGGAATCAGAGAGTTGAGACATTTAAAAATTTGGAGATTTTGTTTTTGAATCGAAATAGGATTAATGATTGGCGTTCCATAAGTGAATTAAACAAACTTAGCTCGTTGAAAAAGCTTTATTTCCTCAAGAATCCGATACAAACCACCGAAGACTACGACACAGGATCGCAGCTGGTTGTCGCTAAAATTGGCACTTTACAGGAATTGAATGGCTCAATAATAACCGGGGAGTTGCGTAGAGGCGCTGAATACGATTACCTGAAGCGTTACGGAGGAGAATGGAAGGTCGCTCAGCTCGATTTTGAGGCGAAAGCGGCCTTCGATGCCGAACATTGTCGCTTTGAGGAGCTCATCCGTAAATACGGAATACCGGAAGACAGCCTCTTGGTCAAGCTGCCGAAAATAACGACGCTGACATCGCAACTTTTAGAAGTAACATTGAGAGACGAAAACGGAAAATCGTTTAAGAAAAAGTTCCCATCTACGATGGCAGTCCAGAAGCTGATCACACTGTCGCAGAGATTGTTCTCGAGAGCCGGCAACACCGGTACACCTCGACTTTACGTACTAGACGAACAGATGGATGGGGCTGAAATATATCTCGACAATTCTATGAAGGATCTCGCTTATTATTCGATTAAAAATGGCGATGTTATTCTAGTTAAATTCAGATAG

Protein

MVTFGVFQTLESQVEFGRSEEELRRVTHTLRVSSVENIAGDLAFDIRKYIGEVQGYKGLWYGIEWDNESRGKHDGCLDGIQYFKTSKPSAGSFVRPNKIIPSKTCAEAIRQYYGDPEDETLAAHRRTIINEWKREMGAPFIEMVGFEKIHQKQKFDRLLEVCVHDQNVSRAGDVSSLCPNVRSLDVSRNLFSNWREVIQLSAQLPDLRDLDVSKNRMLIDAPTEILAQLSINFSKLEKINLSVCDYTWTDILKLSHLWPKIHEIIAAYNRIDEIEPPLVTLRSLSVLRLDGNPITSWSEVMNLGALNLKVLSLNDCLIAEIRFGDDWNQRVETFKNLEILFLNRNRINDWRSISELNKLSSLKKLYFLKNPIQTTEDYDTGSQLVVAKIGTLQELNGSIITGELRRGAEYDYLKRYGGEWKVAQLDFEAKAAFDAEHCRFEELIRKYGIPEDSLLVKLPKITTLTSQLLEVTLRDENGKSFKKKFPSTMAVQKLITLSQRLFSRAGNTGTPRLYVLDEQMDGAEIYLDNSMKDLAYYSIKNGDVILVKFR

Description

Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione.
Tubulin-folding protein; involved in the second step of the tubulin folding pathway and in the regulation of tubulin heterodimer dissociation (PubMed:12389029, PubMed:17184771). Required for correct organization of microtubule cytoskeleton and mitotic splindle, and maintenance of the neuronal microtubule network (By similarity).

Catalytic Activity

(R)-S-lactoylglutathione = glutathione + methylglyoxal

Cofactor

Zn(2+)

Subunit

Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state.
Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state. Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers. Interacts with TBCD (By similarity).

Similarity

Belongs to the glyoxalase I family.
Belongs to the TBCE family.

Keywords

Chaperone   Cytoplasm   Cytoskeleton   Leucine-rich repeat   Repeat   3D-structure   Acetylation   Complete proteome   Phosphoprotein   Polymorphism   Reference proteome  

Feature

chain  Tubulin-specific chaperone E

Uniprot

H9ISC2   A0A2H1WCF3   A0A2A4JYI3   A0A0N1I4V6   A0A2W1BFL5   A0A212FJE5   + More
A0A212EWZ1   A0A194RJW2   A0A158N911   D6WI91   A0A1Y1L5Y1   A0A1Y1L4R6   A0A195BUY8   K7IS98   A0A2C9LD34   A0A154P8U1   A0A0N0BFS8   A0A151WNS7   A0A088ALP0   A0A1W4X421   A0A151J375   A0A0L7QRM2   A0A2A3ETV0   A0A2J7QV38   A0A0J7L128   A0A151K114   E0VEM4   E2BBM1   A0A151IBY3   E2AAG3   A0A232EUB5   A0A310SAK1   A0A1B6EEL5   A0A2J7QV34   A0A0P4XVY7   A0A0P4YSW9   A0A0P6IKA5   V4AGM2   A0A026W415   A0A0P5ZMU4   A0A023F7S1   A0A067RA53   A0A0P6AAN6   A0A1S3IDI9   K7G0W2   A0A1S3ICD7   A0A3B3SA87   A0A0P6DXF3   N6TA25   A0A151M2W4   A0A1B6I484   A0A224XMS2   A0A2D4HA23   A0A0A9Y8G9   A0A0P5CE79   A0A2D4HAA5   A0A0P4VTZ4   A0A1U7RAE4   A0A3B4DXG5   R4GAP8   M3XIN9   A0A060Y2L9   A0A1W7RC53   R4G3X9   V9KB66   A0A1S3L6Y8   R0LCY4   A0A210Q0C9   W5LPV5   T1J621   A0A3P8YIC3   J3S5D9   T1D7I4   A0A0F7Z1T7   W5UCG8   U3IDV7   A0A093RIJ3   A0A1I8NRU6   A0A091G566   H0ZJ32   G3WPP0   A0A1W4VMM1   A0A0P5YR06   A0A1W4VMD9   A0A1L8G8N3   Q5U508   Q8CIV8   W5NG15   A0A091Q4U8   A0A0V0G618   A0A218VAM3   A0A2I0UR94   I3MQ76   B4NYV6   A0A1W4ZSA6   A0A3Q3MKF1   A0A0K8VJ60  

EC Number

4.4.1.5

Pubmed

19121390    26354079    28756777    22118469    21347285    18362917    + More
19820115    28004739    20075255    15562597    20566863    20798317    28648823    23254933    24508170    30249741    25474469    24845553    17381049    29240929    23537049    22293439    25401762    26823975    27129103    9215903    24755649    26358130    24402279    28812685    25329095    25069045    23025625    23758969    23127152    23749191    20360741    21709235    27762356    12389029    16141072    15489334    17184771    21183079    17994087    17550304   

EMBL

BABH01024270    BABH01024271    ODYU01007715    SOQ50743.1    NWSH01000390    PCG76738.1    + More
KQ459286    KPJ02021.1    KZ150604    PZC70463.1    AGBW02008275    OWR53855.1    AGBW02011887    OWR45967.1    KQ460118    KPJ17724.1    ADTU01009023    KQ971321    EFA01432.2    GEZM01068371    JAV66956.1    GEZM01068372    JAV66955.1    KQ976405    KYM91895.1    KQ434827    KZC07638.1    KQ435794    KOX73784.1    KQ982907    KYQ49457.1    KQ980290    KYN16910.1    KQ414775    KOC61278.1    KZ288189    PBC34569.1    NEVH01010479    PNF32445.1    LBMM01001332    KMQ96492.1    KQ981229    KYN44335.1    DS235093    EEB11830.1    GL447128    EFN86898.1    KQ978095    KYM97018.1    GL438125    EFN69564.1    NNAY01002165    OXU21896.1    KQ762190    OAD56160.1    GEDC01020706    GEDC01000958    JAS16592.1    JAS36340.1    PNF32444.1    GDIP01238245    JAI85156.1    GDIP01227634    JAI95767.1    GDIQ01020424    JAN74313.1    KB201847    ESO94310.1    KK107459    QOIP01000004    EZA50346.1    RLU23316.1    GDIP01042597    JAM61118.1    GBBI01001458    JAC17254.1    KK852595    KDR20629.1    GDIP01033202    JAM70513.1    AGCU01078981    AGCU01078982    AGCU01078983    AGCU01078984    AGCU01078985    AGCU01078986    AGCU01078987    GDIQ01070854    JAN23883.1    APGK01038391    APGK01038392    KB740954    KB632338    ENN77099.1    ERL92904.1    AKHW03006780    KYO18826.1    GECU01025960    JAS81746.1    GFTR01006983    JAW09443.1    IACK01016516    LAA68838.1    GBHO01016211    GBRD01015944    GDHC01003000    JAG27393.1    JAG49882.1    JAQ15629.1    GDIP01171832    JAJ51570.1    IACK01016515    IACK01016517    LAA68836.1    GDKW01001113    JAI55482.1    AFYH01046657    AFYH01046658    AFYH01046659    AFYH01046660    AFYH01046661    AFYH01046662    AFYH01046663    AFYH01046664    FR906223    CDQ83639.1    GDAY02002745    JAV48722.1    ACPB03019875    GAHY01001276    JAA76234.1    JW862515    AFO95032.1    KB743543    EOA98157.1    NEDP02005311    OWF42186.1    JH431869    JU176344    AFJ51867.1    GAAZ01002739    JAA95204.1    GBEX01003735    JAI10825.1    JT405921    AHH37382.1    ADON01100450    KL225298    KFW70709.1    KL447787    KFO77088.1    ABQF01014603    AEFK01160097    AEFK01160098    AEFK01160099    AEFK01160100    AEFK01160101    AEFK01160102    GDIP01054864    JAM48851.1    CM004474    OCT80064.1    BC084879    AY082332    AK167383    BC050206    AHAT01001606    KK655550    KFQ04669.1    GECL01002638    JAP03486.1    MUZQ01000016    OWK63097.1    KZ505650    PKU48569.1    AGTP01017245    AGTP01017246    AGTP01017247    AGTP01017248    CM000157    EDW89807.1    GDHF01025707    GDHF01013390    JAI26607.1    JAI38924.1   

Proteomes

UP000005204    UP000218220    UP000053268    UP000007151    UP000053240    UP000005205    + More
UP000007266    UP000078540    UP000002358    UP000076420    UP000076502    UP000053105    UP000075809    UP000005203    UP000192223    UP000078492    UP000053825    UP000242457    UP000235965    UP000036403    UP000078541    UP000009046    UP000008237    UP000078542    UP000000311    UP000215335    UP000030746    UP000053097    UP000279307    UP000027135    UP000085678    UP000007267    UP000261540    UP000019118    UP000030742    UP000050525    UP000189705    UP000261440    UP000001646    UP000008672    UP000193380    UP000015103    UP000087266    UP000242188    UP000018467    UP000265140    UP000221080    UP000016666    UP000054081    UP000095300    UP000053760    UP000007754    UP000007648    UP000192221    UP000186698    UP000000589    UP000018468    UP000197619    UP000005215    UP000002282    UP000192224    UP000261640   

PRIDE

H9ISC2    Q5U508    Q8CIV8   

Pfam

PF01302   CAP_GLY        + More
PF00025   Arf
PF00903   Glyoxalase
PF07717   OB_NTP_bind
PF04408   HA2
PF00271   Helicase_C
PF14560   Ubiquitin_2
PF11625   DUF3253
PF13855   LRR_8

Interpro

IPR001611   Leu-rich_rpt        + More
IPR036859   CAP-Gly_dom_sf       
IPR000938   CAP-Gly_domain       
IPR032675   LRR_dom_sf       
IPR029071   Ubiquitin-like_domsf       
IPR027417   P-loop_NTPase       
IPR006689   Small_GTPase_ARF/SAR       
IPR005225   Small_GTP-bd_dom       
IPR004360   Glyas_Fos-R_dOase_dom       
IPR029068   Glyas_Bleomycin-R_OHBP_Dase       
IPR037523   VOC       
IPR004361   Glyoxalase_1       
IPR018146   Glyoxalase_1_CS       
IPR014001   Helicase_ATP-bd       
IPR001650   Helicase_C       
IPR011709   DUF1605       
IPR002464   DNA/RNA_helicase_DEAH_CS       
IPR007502   Helicase-assoc_dom       
IPR000626   Ubiquitin_dom       
IPR003591   Leu-rich_rpt_typical-subtyp       
IPR021660   DUF3253       
IPR036390   WH_DNA-bd_sf       
IPR036388   WH-like_DNA-bd_sf       

SUPFAM

SSF74924   SSF74924        + More
SSF54236   SSF54236       
SSF52540   SSF52540       
SSF54593   SSF54593       
SSF46785   SSF46785       

Gene 3D

2.30.30.190     3.80.10.10     3.10.180.10     1.10.10.10    

CDD

cd00079  HELICc

ProteinModelPortal

H9ISC2   A0A2H1WCF3   A0A2A4JYI3   A0A0N1I4V6   A0A2W1BFL5   A0A212FJE5   + More
A0A212EWZ1   A0A194RJW2   A0A158N911   D6WI91   A0A1Y1L5Y1   A0A1Y1L4R6   A0A195BUY8   K7IS98   A0A2C9LD34   A0A154P8U1   A0A0N0BFS8   A0A151WNS7   A0A088ALP0   A0A1W4X421   A0A151J375   A0A0L7QRM2   A0A2A3ETV0   A0A2J7QV38   A0A0J7L128   A0A151K114   E0VEM4   E2BBM1   A0A151IBY3   E2AAG3   A0A232EUB5   A0A310SAK1   A0A1B6EEL5   A0A2J7QV34   A0A0P4XVY7   A0A0P4YSW9   A0A0P6IKA5   V4AGM2   A0A026W415   A0A0P5ZMU4   A0A023F7S1   A0A067RA53   A0A0P6AAN6   A0A1S3IDI9   K7G0W2   A0A1S3ICD7   A0A3B3SA87   A0A0P6DXF3   N6TA25   A0A151M2W4   A0A1B6I484   A0A224XMS2   A0A2D4HA23   A0A0A9Y8G9   A0A0P5CE79   A0A2D4HAA5   A0A0P4VTZ4   A0A1U7RAE4   A0A3B4DXG5   R4GAP8   M3XIN9   A0A060Y2L9   A0A1W7RC53   R4G3X9   V9KB66   A0A1S3L6Y8   R0LCY4   A0A210Q0C9   W5LPV5   T1J621   A0A3P8YIC3   J3S5D9   T1D7I4   A0A0F7Z1T7   W5UCG8   U3IDV7   A0A093RIJ3   A0A1I8NRU6   A0A091G566   H0ZJ32   G3WPP0   A0A1W4VMM1   A0A0P5YR06   A0A1W4VMD9   A0A1L8G8N3   Q5U508   Q8CIV8   W5NG15   A0A091Q4U8   A0A0V0G618   A0A218VAM3   A0A2I0UR94   I3MQ76   B4NYV6   A0A1W4ZSA6   A0A3Q3MKF1   A0A0K8VJ60  

PDB

1LPL     E-value=0.000491064,     Score=104

KEGG

101741703    K21768 tubulin-specific chaperone E | (RefSeq) tubulin-specific chaperone E

GO

GO:0005525   GO:0043014   GO:0005737   GO:0007023   GO:0007021   GO:0000226   GO:0004462   GO:0046872   GO:0004771   GO:0004806   GO:0004386   GO:0005524   GO:0009791   GO:0014889   GO:0008344   GO:0048589   GO:0048936   GO:0005856   GO:0007052   GO:0007409   GO:0051082   GO:0006457   GO:0008582   GO:0007274   GO:0046785   GO:0005515   GO:0006468   GO:0004672   GO:0005634   GO:0005507   GO:0004713   GO:0016491   GO:0015930  

Subcellular location

Cytoplasm  
Cytoskeleton  

Length:

550

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00025

Exp number, first 60 AAs:

0

Total prob of N-in:

0.00044

outside

1  -  550

 

 

Pi

230.90818

Theta

158.582112

Tajima's D

2.301262

CLR

0.041241

CSRT

0.92320383980801

Interpretation

Uncertain