Gene

KWMTBOMO14252  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA002161

Annotation

PREDICTED:_cullin-2_[Bombyx_mori]

Location

Bomo_Chr23(-):21435454-21445552      View in EpiBrowser

Full name

Cullin-2      

Location in the cell

Cytoplasmic   Reliability : 1.322 Mitochondrial   Reliability : 1.107 Nuclear   Reliability : 1.088

CDS

ATGATGTCGCTAAAGCCGAGGCACGTGGACTTCCAGGAGACATGGGCCAACCTCAAAGAGACCGTTGCGGGGGTGGTGGGGCTGAAGGCCGTGGACCGCATCGTGTGGAACACCCGCTTCTCTGACGTGTACGCGCTGTGCGTCGCTCACCCCGAGCCCCTGGCGGACCGGCTCTACGACGAGACCAGGAACTTCTTAGAGGAGCACGTGGCAAGTCTGCTGGAGAGAGTTCGAGGCTCCACCACCCTCGAGCAGTCCGACCACAACGATGGACTACTGGACAGGTACGTGTCGGCGTGGCGCGAGTACAGCCAGGGCGTGGCCTACCTCAACAGCCTCTACTCCTACCTCAACCTGCAGCACGTCAAGCGACAGAAGGTCTCCGACGCGGAGATCATATACGGCACGTCGGCCACGGTGAGCTGTCCCGAGCAAGACGCGCGACAACTGGAGGTGGGCGAGCTGGGCCTGGACATCTGGGAGCGCGTGCTGGTGCGGCCGCTGTCCGCCGCCCTCACGGCGCGCATCGTCAGCGCGCTGCACGCCGCCCGGGACGCGCCGCCCGACCCGCGCCACGCCGACATCATCCGACAAGCCATACAGAGCACCGTGCACGTCCGCAGTTTTCGCGTCCGGTCTCCACTCGCGCTGTACGAGCAGCTCGTGCTGGAGCCGTTCCTCCAGGCGGCCGGCGAGCAGCACGCCAGGACGGCGGCCCACTTACTGCAGGAGGGGGACGTCGCACATTACATGAAGCACGTTCTCGCAGGGCTGGAGCGCGAGGTGTCGCTGGCGCAGCGCTACCTGCCGGCGTCGTCCCGCGAGGCGGTCCGCGCGTGCTACGAGCGCGCCGCGGTGGCCGCGCACCTGCCCGCGCTGCACGCCGACGTGGACCGCCTGCTGGCCGAGGCGAGCGCCGACGACGACGACGCGCCCCAGCGCCGCGAGGACTTGTCCCGCATGTACCAGCTGGTGCGGCCCCTGGGCCCGGCGGCGCTGCGGCCGCTGGTGGACGCCGCGCTGGCGCACGCGCAGGCCCACGGACACGCGCTGCTCTCCAGCGCGCTGCAGCACCACAAGGACGAGGTACACATACACTTCGTGGAGGCCATACTGACGCTGCACGGCAAGTACAGCAAGCTGTTCAACGAGGTCTTCGGGGGAGCTCAGGCGTTCATGGGCGCGCTGGACAAGGCCTGCAGCGCCGTGGTGAACAGCCGCAGCGGCGAGGAGGCGTGCCGCGCGCCGGAGCTGGTGGCGCGGTACTGCGACTGGGTGCTCCGCCGCCGCCCCGCCGCCGCGCCCCTCACCGACGTCGACGACAAGCTCACCGCCGCCATCGTCGTCTTCAAGTACATCGACGACAAGGACATCTTCCAGAAGCACTACGCCCGCGCGCTGGCCCGCAGGCTCATCCACCAGCTCTCCTACTCCATGGAGCAGGAGGAGTCGATGATCAACCGTCTGAAGGCGGCGTGCGGCTACGAGTTCACCAACAAGCTGCACCGCATGTTCACCGACGTGGCCGTGTCCGCCGACCTCAACGCCAAATTCCAGCAGTACCTGCGGGACAACGACCTCACGCTCAACACGGGCTTCTTCATCCAGGTGCTGCAGGCGGGCGCGTGGCCGCTGGGGGGCCCCACGGCTCCGCTGGCGCCGCCCGCCGCGCTGGAGCGCCCGGCGGGGCTGTTCGAGCAGTTCTACCGCAGCGCCTTCAGCGGGCGGCGGCTGGCCTGGCTGCACCACCTGTGCACGGGCGAGCTGCGCGTGCGCTACACCCCGCGCGTGTACCACCTCACGGCCAGCACGCCGCAGTGCGCGCTGCTGCTGTCGCTGGAGCGCCGCGACGCCACCACGCTGTCCGAGGCGCGCGCCGCCGCCGTGCTGCCCGAGGACTCCTGGGAGCGCCACGTGCGCCCGCTGCTGGACATCGGCCTGCTGCTGCCGGGCGCCGACCCCGACGAGCTGCTGCTCAACCTGTCCTTCTCGTGCAAGCGCTCCAAGCTGCGGCTGTGCGCGTCCCCGGGCCACGGCGCCGCCGCCTCGCCCCCCGACCCGCCGCAGCACCGCGACGACGACCGCAAGATGTACCTGCAGGCCGCCATCGTGCGCATCATGAAGCAGCGCAAGGTGCTGCGCCACACGGAGCTCATCCAGGAGGTGGTGCAGCAGGCGCGGGGCTCCTTCGCGCCCTCCGTCGCCATCATCAAGAAGTGCATCGAGGCGCTCATCGACAAGCAGTACCTGGAGCGCGCGCCGGGGGCCCTGGACACCTACTCCTACCTGGCCTGA

Protein

MMSLKPRHVDFQETWANLKETVAGVVGLKAVDRIVWNTRFSDVYALCVAHPEPLADRLYDETRNFLEEHVASLLERVRGSTTLEQSDHNDGLLDRYVSAWREYSQGVAYLNSLYSYLNLQHVKRQKVSDAEIIYGTSATVSCPEQDARQLEVGELGLDIWERVLVRPLSAALTARIVSALHAARDAPPDPRHADIIRQAIQSTVHVRSFRVRSPLALYEQLVLEPFLQAAGEQHARTAAHLLQEGDVAHYMKHVLAGLEREVSLAQRYLPASSREAVRACYERAAVAAHLPALHADVDRLLAEASADDDDAPQRREDLSRMYQLVRPLGPAALRPLVDAALAHAQAHGHALLSSALQHHKDEVHIHFVEAILTLHGKYSKLFNEVFGGAQAFMGALDKACSAVVNSRSGEEACRAPELVARYCDWVLRRRPAAAPLTDVDDKLTAAIVVFKYIDDKDIFQKHYARALARRLIHQLSYSMEQEESMINRLKAACGYEFTNKLHRMFTDVAVSADLNAKFQQYLRDNDLTLNTGFFIQVLQAGAWPLGGPTAPLAPPAALERPAGLFEQFYRSAFSGRRLAWLHHLCTGELRVRYTPRVYHLTASTPQCALLLSLERRDATTLSEARAAAVLPEDSWERHVRPLLDIGLLLPGADPDELLLNLSFSCKRSKLRLCASPGHGAAASPPDPPQHRDDDRKMYLQAAIVRIMKQRKVLRHTELIQEVVQQARGSFAPSVAIIKKCIEALIDKQYLERAPGALDTYSYLA

Description

Core component of multiple cullin-RING-based ECS (ElonginB/C-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes, which mediate the ubiquitination of target proteins. ECS complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins (By similarity). May serve as a rigid scaffold in the complex and may contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. The E3 ubiquitin-protein ligase activity of the complex is dependent on the neddylation of the cullin subunit and is inhibited by the association of the deneddylated cullin subunit with TIP120A/CAND1 (By similarity). The functional specificity of the ECS complex depends on the substrate recognition component. ECS(VHL) mediates the ubiquitination of hypoxia-inducible factor (HIF) (By similarity).

Subunit

Component of multiple ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complexes formed of CUL2, Elongin BC (ELOB and ELOC), RBX1 and a variable substrate-specific adapter. Component of the ECS(VHL) or CBC(VHL) complex containing VHL. Component of the ECS(MED8) complex with the probable substrate recognition component MED8. Component of the ECS(LRR1) complex with the probable substrate recognition component LRR1. Component of a probable ECS E3 ubiquitin-protein ligase complex containing CUL2, RBX1, ELOB, ELOC and FEM1B. Part of an E3 ubiquitin-protein ligase complex including ZYG11B, CUL2 and Elongin BC. Part of an E3 ubiquitin-protein ligase complex including ZYG11BL, CUL2 and Elongin BC. Interacts with RBX1, RNF7, FEM1B and TIP120A/CAND1. Interacts with COPS2, and MED8. Found in a complex composed of LIMD1, VHL, EGLN1/PHD2, ELOB and CUL2. Interacts with KLHDC10; which may be an E3 ubiquitin ligase complex substrate recognition component. Interacts (when neddylated) with ARIH1; leading to activate the E3 ligase activity of ARIH1 (By similarity).

Similarity

Belongs to the cullin family.

Keywords

Acetylation   Alternative splicing   Complete proteome   Isopeptide bond   Phosphoprotein   Reference proteome   Ubl conjugation   Ubl conjugation pathway  

Feature

chain  Cullin-2
splice variant  In isoform 2.

Uniprot

A0A194PSD7   A0A212F1N7   A0A2W1B5D8   A0A2H1WKG6   A0A1B6DHH6   A0A1B6LDY5   + More
A0A2J7Q9Q8   A0A1B6HVB3   A0A1B6EIL2   A0A0A9YGX7   A0A2R7VR77   A0A067QY00   A0A0K8SH59   A0A0C9PV90   A0A026VZ64   U5EWS4   A0A0V0GB37   A0A023F3B2   A0A069DWS3   A0A224XJ96   K7J9J0   E9ICR7   A0A232ES04   T1I044   A0A195EZZ3   F4X522   A0A195BPU6   A0A158P2X5   E2C3F0   A0A336LM86   A0A195DNB9   A0A336MXA2   U4UXQ9   A0A151WYK7   A0A1Y1LAV3   N6TVT5   A0A151IIP4   A0A293LDI7   A0A154P1N7   A0A0K2UTU7   D2A5E2   A0A0P5P109   A0A0P5B4N1   A0A0P5S2A1   A0A0P5RH15   E2ALH2   A0A0P5U1P1   A0A0P5PC28   A0A0P5VBD8   A0A310SL12   A0A0P5WV24   A0A0P6FJI6   A0A0P5W4U0   A0A0P4WT17   A0A0P5VAM5   A0A0P5G6V3   A0A2R5LGV2   A0A1L8DSW8   A0A0J7P0J7   A0A0L7RD55   A0A1A9Y0K3   A0A1B0B7K3   A0A087SY48   W8AYD1   A0A1A9VZX7   A0A0K8U2S9   A0A140D042   A0A182QGB2   A0A1A9UXM2   A0A0A1XAX8   A0A0P6F5A2   A0A1Q3G2L9   A0A182WVQ4   A0A182VJQ5   A0A182KXL2   A0A182I9D3   Q7PQH5   A0A023EXR6   T1PHU8   A0A0L0BL23   A0A182YI94   A0A1I8NG86   A0A034VVR3   T1JK90   W5JME3   G5BI23   A0A182P922   Q174D9   A0A0P6HIF3   A0A2M4AJL6   A0A1I8PC49   A0A182J4T9   A0A2M4BEX4   A0A182NFR1   Q571A2   A0A1S4FF02   A0A182FRU2   Q9D4H8   A0A1U7QSN5   S4RCX6  

Pubmed

26354079    22118469    28756777    25401762    24845553    24508170    + More
30249741    25474469    26334808    20075255    21282665    28648823    21719571    21347285    20798317    23537049    28004739    18362917    19820115    24495485    26864195    25830018    20966253    12364791    14747013    17210077    24945155    26108605    25244985    25315136    25348373    20920257    23761445    21993625    17510324    16141072    15489334    11967155    12149480    21183079    23806337   

EMBL

KQ459593    KPI96351.1    AGBW02010838    OWR47655.1    KZ150513    PZC70631.1    + More
ODYU01009228    SOQ53497.1    GEDC01012196    JAS25102.1    GEBQ01018085    JAT21892.1    NEVH01016344    PNF25321.1    GECU01029327    GECU01029126    JAS78379.1    JAS78580.1    GECZ01032011    JAS37758.1    GBHO01011287    JAG32317.1    KK854031    PTY09849.1    KK853123    KDR11056.1    GBRD01013183    JAG52643.1    GBYB01005318    JAG75085.1    KK107570    QOIP01000005    EZA48756.1    RLU22941.1    GANO01000425    JAB59446.1    GECL01001623    JAP04501.1    GBBI01002807    JAC15905.1    GBGD01000608    JAC88281.1    GFTR01007896    JAW08530.1    AAZX01017353    GL762354    EFZ21628.1    NNAY01002518    OXU21148.1    ACPB03011808    KQ981905    KYN33444.1    GL888680    EGI58479.1    KQ976432    KYM87517.1    ADTU01007640    GL452320    EFN77488.1    UFQS01002888    UFQT01002888    SSX14832.1    SSX34222.1    KQ980713    KYN14400.1    UFQT01002906    SSX34251.1    KB632404    ERL95080.1    KQ982649    KYQ52968.1    GEZM01061125    JAV70764.1    APGK01052870    KB741216    ENN72501.1    KQ977459    KYN02555.1    GFWV01001208    MAA25938.1    KQ434796    KZC05846.1    HACA01024099    CDW41460.1    KQ971345    EFA05359.1    GDIQ01136728    JAL14998.1    GDIP01189726    JAJ33676.1    GDIQ01093277    JAL58449.1    GDIQ01107122    GDIP01089705    GDIQ01072547    GDIQ01047702    JAL44604.1    JAM14010.1    GL440607    EFN65730.1    GDIP01120983    GDIP01119036    JAL84678.1    GDIQ01152364    JAK99361.1    GDIQ01214686    GDIQ01107123    GDIP01101984    JAK37039.1    JAM01731.1    KQ762882    OAD55361.1    GDIP01081390    JAM22325.1    GDIQ01056447    JAN38290.1    GDIP01104832    JAL98882.1    GDIP01251216    JAI72185.1    GDIP01104833    JAL98881.1    GDIQ01246426    JAK05299.1    GGLE01004620    MBY08746.1    GFDF01004536    JAV09548.1    LBMM01000518    KMQ98150.1    KQ414615    KOC68691.1    JXJN01009682    KK112489    KFM57787.1    GAMC01015428    GAMC01015427    JAB91127.1    GDHF01031538    GDHF01030394    GDHF01012864    GDHF01012565    JAI20776.1    JAI21920.1    JAI39450.1    JAI39749.1    KU175340    AMJ52355.1    AXCN02000243    GBXI01015058    GBXI01006015    JAC99233.1    JAD08277.1    GDIQ01053711    JAN41026.1    GFDL01001001    JAV34044.1    APCN01003257    AAAB01008888    EAA08832.5    GAPW01000299    JAC13299.1    KA647700    AFP62329.1    JRES01001704    KNC20747.1    GAKP01012781    GAKP01012780    JAC46172.1    JH431641    ADMH02000631    ETN65547.1    JH170408    EHB08934.1    CH477413    EAT41424.1    GDIQ01019173    JAN75564.1    GGFK01007633    MBW40954.1    GGFJ01002402    MBW51543.1    AK220287    BAD90212.1    AK016520    AK160597    BC026779    BC027428    BC025902   

Proteomes

UP000053268    UP000007151    UP000235965    UP000027135    UP000053097    UP000279307    + More
UP000002358    UP000215335    UP000015103    UP000078541    UP000007755    UP000078540    UP000005205    UP000008237    UP000078492    UP000030742    UP000075809    UP000019118    UP000078542    UP000076502    UP000007266    UP000000311    UP000036403    UP000053825    UP000092443    UP000092460    UP000054359    UP000091820    UP000075886    UP000078200    UP000076407    UP000075903    UP000075882    UP000075840    UP000007062    UP000037069    UP000076408    UP000095301    UP000000673    UP000006813    UP000075885    UP000008820    UP000095300    UP000075880    UP000075884    UP000069272    UP000000589    UP000189706    UP000245300   

PRIDE

Q571A2    Q9D4H8   

Pfam

PF00888   Cullin        + More
PF10557   Cullin_Nedd8

Interpro

IPR036317   Cullin_homology_sf        + More
IPR016158   Cullin_homology       
IPR016159   Cullin_repeat-like_dom_sf       
IPR036390   WH_DNA-bd_sf       
IPR001373   Cullin_N       
IPR036388   WH-like_DNA-bd_sf       
IPR019559   Cullin_neddylation_domain       
IPR016157   Cullin_CS       

SUPFAM

SSF46785   SSF46785        + More
SSF74788   SSF74788       
SSF75632   SSF75632       

Gene 3D

1.10.10.10    

ProteinModelPortal

A0A194PSD7   A0A212F1N7   A0A2W1B5D8   A0A2H1WKG6   A0A1B6DHH6   A0A1B6LDY5   + More
A0A2J7Q9Q8   A0A1B6HVB3   A0A1B6EIL2   A0A0A9YGX7   A0A2R7VR77   A0A067QY00   A0A0K8SH59   A0A0C9PV90   A0A026VZ64   U5EWS4   A0A0V0GB37   A0A023F3B2   A0A069DWS3   A0A224XJ96   K7J9J0   E9ICR7   A0A232ES04   T1I044   A0A195EZZ3   F4X522   A0A195BPU6   A0A158P2X5   E2C3F0   A0A336LM86   A0A195DNB9   A0A336MXA2   U4UXQ9   A0A151WYK7   A0A1Y1LAV3   N6TVT5   A0A151IIP4   A0A293LDI7   A0A154P1N7   A0A0K2UTU7   D2A5E2   A0A0P5P109   A0A0P5B4N1   A0A0P5S2A1   A0A0P5RH15   E2ALH2   A0A0P5U1P1   A0A0P5PC28   A0A0P5VBD8   A0A310SL12   A0A0P5WV24   A0A0P6FJI6   A0A0P5W4U0   A0A0P4WT17   A0A0P5VAM5   A0A0P5G6V3   A0A2R5LGV2   A0A1L8DSW8   A0A0J7P0J7   A0A0L7RD55   A0A1A9Y0K3   A0A1B0B7K3   A0A087SY48   W8AYD1   A0A1A9VZX7   A0A0K8U2S9   A0A140D042   A0A182QGB2   A0A1A9UXM2   A0A0A1XAX8   A0A0P6F5A2   A0A1Q3G2L9   A0A182WVQ4   A0A182VJQ5   A0A182KXL2   A0A182I9D3   Q7PQH5   A0A023EXR6   T1PHU8   A0A0L0BL23   A0A182YI94   A0A1I8NG86   A0A034VVR3   T1JK90   W5JME3   G5BI23   A0A182P922   Q174D9   A0A0P6HIF3   A0A2M4AJL6   A0A1I8PC49   A0A182J4T9   A0A2M4BEX4   A0A182NFR1   Q571A2   A0A1S4FF02   A0A182FRU2   Q9D4H8   A0A1U7QSN5   S4RCX6  

PDB

5N4W     E-value=8.64045e-144,     Score=1311

KEGG

101739865    K03870 cullin 2 | (RefSeq) cullin-2

PATHWAY

04120    Ubiquitin mediated proteolysis - Bombyx mori (domestic silkworm)

GO

GO:0031625   GO:0031461   GO:0006511   GO:0043161   GO:0031462   GO:0019005   GO:0031146   GO:0016874   GO:0030891   GO:0005654   GO:0030163   GO:0005730   GO:0016567   GO:0044877   GO:0001944   GO:0043009   GO:0016791   GO:0005488   GO:0005507   GO:0004713   GO:0016491   GO:0015930  

Length:

764

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0025

Exp number, first 60 AAs:

0.00084

Total prob of N-in:

0.00019

outside

1  -  764

 

 

Pi

4.361687

Theta

15.885416

Tajima's D

-1.888614

CLR

1.897264

CSRT

0.0205489725513724

Interpretation

Uncertain