Gene

KWMTBOMO14141  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA011188

Annotation

PREDICTED:_nitrilase_homolog_1_isoform_X2_[Bombyx_mori]

Location

Bomo_Chr23(-):19188410-19196475      View in EpiBrowser

Full name

Nitrilase and fragile histidine triad fusion protein NitFhit      

Alternative Name

NFT-1 protein

Location in the cell

Cytoplasmic   Reliability : 2.699

CDS

ATGTCTAGTAAAAGGATAGCTGTCTGTCAAATGACTTCTGTTGCTGACAAAGCGGCCAATTTAAAAGTTGTCGAAGGCATCATTGATAGTGCTGCCAAAGAAAATGTTCAGATGCTTTTCTTTCCAGAAGCATGCGACTACATCTGTGACAACAAAAAGGATATAGTAAACTTTTCTGAGCCTATATTTGGTGGTAAGATTGTGGGAAAGTATAGAGAATTGGCAGAGAAATATGGAGTTTGGTTGTCTATGGGAGGGGTTCACGAAAGGGATGAAAAAAATCCAAACAAAATGTACAACACGCACATAATAATAGATGACAAAGGATCCCTTGTACAAATGTACAGGAAACTACATCTTTTCGATGTAGAGATACCGGAGAGAAACGTTAGATTGAAGGAGAGTGACTTCTCGAATGCTGGCGACCATATTGTGGCACCAGTCGATACTCCTGTAGGGAAAATTGGTATGGCTATATGTTATGATATGCGCTTCCCTGAGCTGAGTACATCCCTGAGTATAATGAGCGCCGACATCTTGACATTTCCATCGGCTTTCACTCAAGCAACAGGGGAGGCTCACTGGCATATATTACTCAGATCAAGGGCGATAGAAAACCAATGCTACGTGGTTGCAGCAGCTCAGTCCGGCGTTCACAACCCGAAGAGGCGTTCGTACGGGCACGCGCTCTGTGTCGACCCGTGGGGCGAAGTCCTGGCCGACTGCGGGGAGTCCGCCCCCTGCTATAAAATCGTTGAGATATCTCATGACAAACTGAATGATGTGAGACGTAACATGCCCGTATTTCAGCATAGAAGACCTGACGTGTATTCCTTGTACACTTTGTCTTTACGCAAGTCCCTCCCGTTAGAGAAGCTGCCATCCCACTCCCCCACCGACCCCAACCCCCCTCCGTCCTCCCCAGAGCCGTACAACGTCTTCGGTCACGCCAAGATCCCGGAGAGCTGCATTTTCTTCAAGTCCAAATTCGCTTACGCCTTTGTCAATCTGAGATGTGTGATCCCTGGGCACGTCCTGGTGGCGCCCATCAGGCCTGCCGAGAGGAACAAGGATCTCACCGAGGAGGAGGCCGAAGATTTCTTCAGAACCGTTCGTTTGGTCCAAAAGCTGATGGAGAAGGTGCATGGTGCAGAGTCTTGCACGATAACGATACAAGACGGACCTGACGCGGGGCAGACGGTCAAGCATCTTCATTGTCACGTGATGCCGCGGAAGAAGGGAGATTTTATAGACAACGATTTAATATACCTGGAATTGGCGAAGCATGATCAATTGAAGTGCGGTCACCCGTTGAAGCCGGCCCGAAGTTTGGAAGAGAGACGAAGCGAAGCTCAACTTCTCAGAAGCGAATTGGAGAAGTTGATCGACGAGGAACGCATATTGCTAAGATAA

Protein

MSSKRIAVCQMTSVADKAANLKVVEGIIDSAAKENVQMLFFPEACDYICDNKKDIVNFSEPIFGGKIVGKYRELAEKYGVWLSMGGVHERDEKNPNKMYNTHIIIDDKGSLVQMYRKLHLFDVEIPERNVRLKESDFSNAGDHIVAPVDTPVGKIGMAICYDMRFPELSTSLSIMSADILTFPSAFTQATGEAHWHILLRSRAIENQCYVVAAAQSGVHNPKRRSYGHALCVDPWGEVLADCGESAPCYKIVEISHDKLNDVRRNMPVFQHRRPDVYSLYTLSLRKSLPLEKLPSHSPTDPNPPPSSPEPYNVFGHAKIPESCIFFKSKFAYAFVNLRCVIPGHVLVAPIRPAERNKDLTEEEAEDFFRTVRLVQKLMEKVHGAESCTITIQDGPDAGQTVKHLHCHVMPRKKGDFIDNDLIYLELAKHDQLKCGHPLKPARSLEERRSEAQLLRSELEKLIDEERILLR

Description

Cleaves A-5'-PPP-5'A to yield AMP and ADP.

Catalytic Activity

H2O + P(1),P(3)-bis(5'-adenosyl) triphosphate = ADP + AMP + 2 H(+)

Cofactor

Mn(2+)

Subunit

Homotetramer.

Similarity

In the N-terminal section; belongs to the UPF0012 family.

Keywords

Complete proteome   Hydrolase   Manganese   Multifunctional enzyme   Nucleotide-binding   Reference proteome  

Feature

chain  Nitrilase and fragile histidine triad fusion protein NitFhit

Uniprot

H9JNT4   A0A3S2M9X3   A0A194PYW8   A0A2W1BMV7   A0A212EYD9   A0A2A4JM41   + More
A0A2A4JM72   A0A2A4JK95   A0A2A4JKK9   A0A194RN05   A0A2H1WU17   A0A1B6DP50   B0X0L8   Q17CS4   A0A1W4W725   A0A1S4FYD0   Q16KC3   A0A1W4W719   A0A084WIC3   A0A1Y1MLF3   A0A182MYV1   A0A182WF00   A0A182JKL3   A0A182MAY5   A0A2M4APY5   A0A2M4BMK6   A0A2M4BN38   A0A2M4BN41   A0A2M4BNI5   A0A2M4BLN9   A0A1B6E6R9   A0A182QBF2   A0A2M4APS5   A0A182T378   A0A2M4BLG9   A0A336L9P9   W5JI67   A0A182UVD7   Q7PV69   A0A182PKB9   D2A1E5   A0A336KP34   A0A182FBA8   A0A182HQB1   A0A182RFF5   A0A182U2Z7   A0A182Y1J6   A0A182WZR6   A0A1S3I4Y3   A0A182KSD2   A0A0N4UVW9   A0A182G9I2   E9HV92   J3JUQ9   A0A0P5E5D1   A0A0P6H994   A0A182JXE5   A0A1B0CL19   A0A1B6MAR1   A0A2R5LGW8   A0A1L8E2Q3   A0A087T828   B4IZR5   U4UYT5   A0A1I8N324   T1PFZ5   B4HVC3   B4QKU1   A0A1W4W0F5   B3M4Y4   O76464   A0A067QS78   A0A2A2JVC8   A0A238BK31   A0A1I8P4N4   A0A183HY68   J9EQ30   A0A0N4TMT2   A0A1B0BYJ6   A0A2A2LTH4   A0A1B0G9E8   B4KWR0   A0A3P6T7F8   B5DQY7   B4H5C6   A0A0J9Y2F3   A0A1A9XVN6   D3TNS9   A0A044TJV1   A0A1A9VGC5   B4PC25   A0A0M4EYJ4   B3NEL4   A0A0P6BW84   A0A1D1VY80   A0A182DX07   A0A0L0CDX4   X1WQ65   A0A1I7VVT3  

Pubmed

19121390    26354079    28756777    22118469    17510324    24438588    + More
28004739    20920257    23761445    12364791    14747013    17210077    18362917    19820115    25244985    20966253    26483478    21292972    22516182    17994087    23537049    25315136    22936249    9671749    10731132    12537572    12537569    24845553    15632085    17885136    20353571    17550304    18057021    27649274    22919073    26108605    25217238   

EMBL

BABH01009991    BABH01009992    RSAL01000005    RVE54405.1    KQ459593    KPI96350.1    + More
KZ149959    PZC76389.1    AGBW02011569    OWR46477.1    NWSH01001122    PCG72493.1    PCG72492.1    PCG72495.1    PCG72491.1    KQ460152    KPJ17381.1    ODYU01011062    SOQ56560.1    GEDC01021800    GEDC01009844    JAS15498.1    JAS27454.1    DS232241    EDS38179.1    CH477304    EAT44198.1    CH477969    EAT34733.1    ATLV01023928    KE525347    KFB49967.1    GEZM01032248    JAV84726.1    AXCM01003135    GGFK01009525    MBW42846.1    GGFJ01004867    MBW54008.1    GGFJ01005355    MBW54496.1    GGFJ01005356    MBW54497.1    GGFJ01005197    MBW54338.1    GGFJ01004858    MBW53999.1    GEDC01003683    JAS33615.1    AXCN02001021    GGFK01009446    MBW42767.1    GGFJ01004731    MBW53872.1    UFQS01002180    UFQT01002180    SSX13433.1    SSX32864.1    ADMH02001330    ETN62978.1    AAAB01008986    EAA00382.4    KQ971338    EFA01543.1    UFQS01000654    UFQT01000654    SSX05808.1    SSX26167.1    APCN01003305    UXUI01007196    VDD86172.1    JXUM01154242    KQ571901    KXJ68098.1    GL732844    EFX64342.1    BT126974    AEE61936.1    GDIP01147846    LRGB01002443    JAJ75556.1    KZS07687.1    GDIQ01022626    JAN72111.1    AJWK01016847    GEBQ01006979    JAT32998.1    GGLE01004645    MBY08771.1    GFDF01001175    JAV12909.1    KK113888    KFM61267.1    CH916366    EDV95650.1    KB632433    ERL95561.1    KA646808    AFP61437.1    CH480817    EDW49888.1    CM000363    CM002912    EDX08687.1    KMY96588.1    CH902618    EDV40558.2    AF069989    AE014296    AY089221    AAC39137.1    AAF47347.1    AAL89959.1    KK853018    KDR12452.1    LIAE01010201    PAV65628.1    KZ271527    OZC05226.1    UZAJ01039841    VDP11299.1    ADBV01001549    EJW84596.1    UZAD01013163    VDN90917.1    JXJN01022680    LIAE01006444    PAV89531.1    CCAG010017460    CH933809    EDW17507.2    UYRX01000282    VDK79219.1    CH379070    EDY73974.2    CH479211    EDW32978.1    LN857014    CDQ00916.2    EZ423081    ADD19357.1    CMVM020000146    CM000159    EDW92680.2    CP012525    ALC43587.1    CH954178    EDV50009.1    KQS43006.1    GDIP01008737    JAM94978.1    BDGG01000009    GAV03569.1    UYRW01000014    VDK61546.1    JRES01000501    KNC30613.1    ABLF02028319   

Proteomes

UP000005204    UP000283053    UP000053268    UP000007151    UP000218220    UP000053240    + More
UP000002320    UP000008820    UP000192223    UP000030765    UP000075884    UP000075920    UP000075880    UP000075883    UP000075886    UP000075901    UP000000673    UP000075903    UP000007062    UP000075885    UP000007266    UP000069272    UP000075840    UP000075900    UP000075902    UP000076408    UP000076407    UP000085678    UP000075882    UP000038041    UP000274131    UP000069940    UP000249989    UP000000305    UP000076858    UP000075881    UP000092461    UP000054359    UP000001070    UP000030742    UP000095301    UP000001292    UP000000304    UP000192221    UP000007801    UP000000803    UP000027135    UP000218231    UP000095300    UP000050787    UP000004810    UP000038020    UP000278627    UP000092460    UP000092444    UP000009192    UP000277928    UP000001819    UP000008744    UP000092443    UP000024404    UP000078200    UP000002282    UP000092553    UP000008711    UP000186922    UP000077448    UP000271087    UP000037069    UP000007819    UP000095285   

PRIDE

H9JNT4    O76464   

Pfam

PF01230   HIT        + More
PF00795   CN_hydrolase
PF00160   Pro_isomerase

Interpro

IPR003010   C-N_Hydrolase        + More
IPR036526   C-N_Hydrolase_sf       
IPR036265   HIT-like_sf       
IPR001310   Histidine_triad_HIT       
IPR001110   UPF0012_CS       
IPR011146   HIT-like       
IPR039383   FHIT       
IPR019808   Histidine_triad_CS       
IPR002130   Cyclophilin-type_PPIase_dom       
IPR029000   Cyclophilin-like_dom_sf       
IPR020892   Cyclophilin-type_PPIase_CS       

SUPFAM

SSF56317   SSF56317        + More
SSF54197   SSF54197       
SSF50891   SSF50891       

Gene 3D

3.30.428.10     3.60.110.10     2.40.100.10    

CDD

cd01275  FHIT

ProteinModelPortal

H9JNT4   A0A3S2M9X3   A0A194PYW8   A0A2W1BMV7   A0A212EYD9   A0A2A4JM41   + More
A0A2A4JM72   A0A2A4JK95   A0A2A4JKK9   A0A194RN05   A0A2H1WU17   A0A1B6DP50   B0X0L8   Q17CS4   A0A1W4W725   A0A1S4FYD0   Q16KC3   A0A1W4W719   A0A084WIC3   A0A1Y1MLF3   A0A182MYV1   A0A182WF00   A0A182JKL3   A0A182MAY5   A0A2M4APY5   A0A2M4BMK6   A0A2M4BN38   A0A2M4BN41   A0A2M4BNI5   A0A2M4BLN9   A0A1B6E6R9   A0A182QBF2   A0A2M4APS5   A0A182T378   A0A2M4BLG9   A0A336L9P9   W5JI67   A0A182UVD7   Q7PV69   A0A182PKB9   D2A1E5   A0A336KP34   A0A182FBA8   A0A182HQB1   A0A182RFF5   A0A182U2Z7   A0A182Y1J6   A0A182WZR6   A0A1S3I4Y3   A0A182KSD2   A0A0N4UVW9   A0A182G9I2   E9HV92   J3JUQ9   A0A0P5E5D1   A0A0P6H994   A0A182JXE5   A0A1B0CL19   A0A1B6MAR1   A0A2R5LGW8   A0A1L8E2Q3   A0A087T828   B4IZR5   U4UYT5   A0A1I8N324   T1PFZ5   B4HVC3   B4QKU1   A0A1W4W0F5   B3M4Y4   O76464   A0A067QS78   A0A2A2JVC8   A0A238BK31   A0A1I8P4N4   A0A183HY68   J9EQ30   A0A0N4TMT2   A0A1B0BYJ6   A0A2A2LTH4   A0A1B0G9E8   B4KWR0   A0A3P6T7F8   B5DQY7   B4H5C6   A0A0J9Y2F3   A0A1A9XVN6   D3TNS9   A0A044TJV1   A0A1A9VGC5   B4PC25   A0A0M4EYJ4   B3NEL4   A0A0P6BW84   A0A1D1VY80   A0A182DX07   A0A0L0CDX4   X1WQ65   A0A1I7VVT3  

PDB

1EMS     E-value=1.05237e-107,     Score=998

KEGG

101742118    K11206 deaminated glutathione amidase [EC:3.5.1.128] | (RefSeq) nitrilase homolog 1

GO

GO:0006807   GO:0003824   GO:0016787   GO:0047710   GO:0006139   GO:0016810   GO:0000166   GO:0003755   GO:0006457   GO:0008017   GO:0016887   GO:0019829   GO:0005507   GO:0004713   GO:0016491   GO:0015930  

Length:

470

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.08305

Exp number, first 60 AAs:

0.00044

Total prob of N-in:

0.01491

outside

1  -  470

 

 

Pi

18.619954

Theta

19.046179

Tajima's D

0.007454

CLR

0.814746

CSRT

0.378681065946703

Interpretation

Uncertain