Gene

KWMTBOMO13997  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA011342

Annotation

fibroinase_precursor_[Bombyx_mori]

Location

Bomo_Chr23(+):15654322-15664685      View in EpiBrowser

Full name

Cathepsin L      

Alternative Name

Cysteine proteinase 1

Location in the cell

Lysosomal   Reliability : 2.79

CDS

ATGAAGTGTTTAGTATTGCTGCTATGCGCAGTGGCTGCTGTGAGTGCTGTTCAGTTCTTTGACCTGGTCAAGGAAGAGTGGAGTGCCTTCAAGCTGCAGCACCGTCTCAACTACGAAAGCGAGGTCGAAGACAATTTCCGCATGAAGATATACGCTGAGCACAAGCACATCATCGCCAAACACAACCAGAAGTACGAAATGGGCCTCGTTTCTTACAAGCTGGGCATGAACAAGTACGGAGACATGCTCCACCACGAGTTCGTGAAGACTATGAACGGCTTCAACAAAACTGCCAAACACAACAAGAATCTGTACATGAAGGGTGGGAGCGTCCGCGGGGCTAAGTTCATATCGCCGGCCAACGTGAAGCTGCCGGAGCAGGTGGACTGGAGGAAGCACGGCGCCGTCACCGACATCAAGGACCAAGGGAAGTGTGGCTCATGCTGGTCCTTCAGCACGACTGGAGCTTTGGAAGGACAGCACTTCCGTCAGTCCGGCTACCTGGTGTCGCTCTCGGAGCAAAACCTCATCGACTGCTCGGAGCAGTACGGGAACAACGGCTGCAACGGGGGGCTCATGGACAACGCCTTCAAGTACATCAAGGACAACGGGGGCATCGACACCGAGCAGACCTACCCCTACGAGGGAGTTGACGACAAGTGCAGGTACAATCCCAAGAACACCGGTGCTGAGGACGTGGGCTTCGTGGACATCCCCGAGGGCGACGAACAGAAGCTGATGGAAGCCGTGGCCACCGTGGGGCCCGTCTCCGTGGCCATCGACGCCTCGCACACCAGCTTCCAGCTCTACTCCAGCGGAGTCTACAACGAGGAGGAGTGCTCCTCCACTGACCTGGACCACGGGGTTCTGGTGGTGGGTTACGGCACCGACGAGCAGGGCGTGGACTACTGGCTCGTGAAGAACTCGTGGGGCCGCTCGTGGGGCGAGCTGGGCTACATCAAGATGATCCGGAACAAGAACAACCGCTGCGGCATCGCCTCCTCCGCCTCCTACCCCCTCGTGTAA

Protein

MKCLVLLLCAVAAVSAVQFFDLVKEEWSAFKLQHRLNYESEVEDNFRMKIYAEHKHIIAKHNQKYEMGLVSYKLGMNKYGDMLHHEFVKTMNGFNKTAKHNKNLYMKGGSVRGAKFISPANVKLPEQVDWRKHGAVTDIKDQGKCGSCWSFSTTGALEGQHFRQSGYLVSLSEQNLIDCSEQYGNNGCNGGLMDNAFKYIKDNGGIDTEQTYPYEGVDDKCRYNPKNTGAEDVGFVDIPEGDEQKLMEAVATVGPVSVAIDASHTSFQLYSSGVYNEEECSSTDLDHGVLVVGYGTDEQGVDYWLVKNSWGRSWGELGYIKMIRNKNNRCGIASSASYPLV

Description

Important for the overall degradation of proteins in lysosomes. Required for differentiation of imaginal disks.
Important for the overall degradation of proteins in lysosomes. Essential for adult male and female fertility. May play a role in digestion.

Catalytic Activity

Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.

Subunit

Dimer of a heavy and a light chain linked by disulfide bonds.

Similarity

Belongs to the peptidase C1 family.

Keywords

Developmental protein   Differentiation   Direct protein sequencing   Disulfide bond   Glycoprotein   Hydrolase   Lysosome   Protease   Signal   Thiol protease   Zymogen   Alternative splicing   Complete proteome   Digestion   Reference proteome  

Feature

propeptide  Activation peptide
chain  Cathepsin L heavy chain
splice variant  In isoform A.

Uniprot

Q6T9Z7   Q26425   C7BWY7   A0A212FHR9   A0A2A4K1J7   A0A2H1VVV9   + More
A0A023J7N9   E2J8A8   I4DJ47   Q6UB44   F8V2Y5   A0A0N0PBH9   S4PHD4   A1DYI6   I4DM20   A0A1E1WFC2   K7J502   U5ET77   A0A194QJW0   Q26636   A0A1W4X5C5   A0A0N9ZTP4   A0A0L7RDJ1   A0A1B0EZ73   A7XH36   A0A1L8DPC9   A0A1L8DPN1   B4KTB9   A0A232F4K0   Q86FS6   C5I793   A0A0M4EVM8   D6X516   A0A1B0CQU1   Q95V59   A0A151JSU9   A0A0C9PTJ9   Q95029-2   Q95029   C6SV44   A0A1W4VQY0   A0A0L0C185   A0A088ARP1   B3NRF0   B4P445   B4JW16   A0A2A3E2D8   V9IF08   A0A0A1XMN3   A0A3B0JLP7   Q7YXL4   B4QEW3   A0A195DTS2   Q7YXL3   B4HQZ0   B4LJE9   E9IEP8   Q28XK9   B4GGA5   R4UMJ7   A0A310SMP9   A0A034W1P8   E2C9C0   O46030   A0A1V1FVL6   A0A067R0U6   V5GVR8   A0A3B0J0I7   A0A182M3E8   B3MHG5   A0A182RYY3   A0A1L8EHK8   A0A195ATU0   A0A1L8EHE3   A0A1D1ZBY8   B4MNQ5   A0A182W1X4   A0A2J7PV32   A0A0U1ZDE6   A0A182J2Y7   A0A336LNC5   A0A182QBB7   A0A158NPU2   T1PFE3   B0WI10   U4U785   N6UN71   A0A1Q3FIJ1   Q17H05   A0A182P7H9   A0A182N3W2   R4FQ93   W8BGA5   T1D4A5   A0A2M3YZP6   A0A2M4BSK6   A0A2M3YZN8   A0A1S4F2V5   J3JY43   A0A023ENZ2  

EC Number

3.4.22.15

Pubmed

19121390    7706225    19361282    22118469    24467606    22651552    + More
26354079    23622113    20075255    8195162    17994087    28648823    15944084    19481148    18362917    19820115    9099581    9662479    10731132    12537572    7851441    26108605    17550304    25830018    22936249    21282665    15632085    25348373    20798317    9133615    28410430    24845553    21347285    25315136    23537049    17510324    24495485    24330624    22516182    24945155   

EMBL

BABH01009669    BABH01009670    AY426971    AAR87763.1    S77508    AAB33990.1    + More
FM958000    CAX16635.1    AGBW02008466    OWR53275.1    NWSH01000235    PCG78141.1    ODYU01004593    SOQ44632.1    KC896759    AHG99253.1    HQ110065    ADN19567.1    AK401315    BAM17937.1    AY373973    AAQ75437.1    JN017204    AEJ31938.1    KQ460973    KPJ10096.1    GAIX01003317    JAA89243.1    EF068256    ABK90824.1    AK402338    BAM18960.1    GDQN01005354    GDQN01001456    JAT85700.1    JAT89598.1    GANO01002905    JAB56966.1    KQ459144    KPJ03741.1    D16533    KR534638    ALI57394.1    KQ414614    KOC68850.1    AJVK01007787    EF488012    ABR88030.1    GFDF01005778    JAV08306.1    GFDF01005779    JAV08305.1    CH933808    EDW10631.1    NNAY01000962    OXU25714.1    AY207373    AAO48766.2    FJ763762    ACR56863.1    CP012524    ALC41950.1    KQ971381    EEZ97696.1    AJWK01024035    AF426414    AAL16954.1    KQ982014    KYN30638.1    GBYB01004688    JAG74455.1    U75652    AF012089    AE013599    BT016071    D31970    BT089036    ACU12382.1    JRES01001139    KNC25214.1    CH954179    EDV56102.1    CM000158    EDW90555.1    CH916375    EDV98154.1    KZ288427    PBC25858.1    JR040710    JR040711    AEY59252.1    AEY59253.1    GBXI01002031    JAD12261.1    OUUW01000001    SPP74166.1    AY332270    KP303276    AAP94046.1    AJF94874.1    CM000362    CM002911    EDX06990.1    KMY93646.1    KQ980487    KYN15924.1    AY332271    AAP94047.1    CH480816    EDW47787.1    CH940648    EDW60529.1    GL762655    EFZ20954.1    CM000071    EAL26307.1    CH479183    EDW35525.1    KC571836    AGM32335.1    KQ760869    OAD58758.1    GAKP01010710    JAC48242.1    GL453806    EFN75465.1    D82884    BAA24442.1    FX985415    BAX07428.1    KK853019    KDR12407.1    GALX01004128    JAB64338.1    SPP74167.1    AXCM01000489    CH902619    EDV37965.2    GFDG01000710    JAV18089.1    KQ976745    KYM75472.1    GFDG01000711    JAV18088.1    GDJX01003544    JAT64392.1    CH963848    EDW73744.1    NEVH01020989    PNF20196.1    KJ913663    AJS13771.1    UFQS01002236    UFQT01000092    UFQT01002236    SSX13560.1    SSX19582.1    AXCN02001095    ADTU01022754    KA646890    AFP61519.1    DS231941    EDS28092.1    KB631815    ERL86466.1    APGK01015962    KB739708    ENN82151.1    GFDL01007699    JAV27346.1    CH477254    EAT45919.1    ACPB03002359    GAHY01000534    JAA76976.1    GAMC01006215    JAC00341.1    GALA01001028    JAA93824.1    GGFM01000986    MBW21737.1    GGFJ01006924    MBW56065.1    GGFM01000950    MBW21701.1    BT128167    AEE63128.1    GAPW01002427    GAPW01002426    JAC11172.1   

Proteomes

UP000005204    UP000007151    UP000218220    UP000053240    UP000002358    UP000053268    + More
UP000192223    UP000053825    UP000092462    UP000009192    UP000215335    UP000092553    UP000007266    UP000092461    UP000078541    UP000000803    UP000192221    UP000037069    UP000005203    UP000008711    UP000002282    UP000001070    UP000242457    UP000268350    UP000000304    UP000078492    UP000001292    UP000008792    UP000001819    UP000008744    UP000008237    UP000027135    UP000075883    UP000007801    UP000075900    UP000078540    UP000007798    UP000075920    UP000235965    UP000075880    UP000075886    UP000005205    UP000095301    UP000002320    UP000030742    UP000019118    UP000008820    UP000075885    UP000075884    UP000015103   

PRIDE

Q6T9Z7    Q26425    Q26636    A7XH36    Q95029    C6SV44    + More

Pfam

PF08246   Inhibitor_I29        + More
PF00112   Peptidase_C1

Interpro

IPR025661   Pept_asp_AS        + More
IPR000668   Peptidase_C1A_C       
IPR000169   Pept_cys_AS       
IPR013201   Prot_inhib_I29       
IPR039417   Peptidase_C1A_papain-like       
IPR038765   Papain-like_cys_pep_sf       
IPR025660   Pept_his_AS       

SUPFAM

SSF54001   SSF54001       

CDD

cd02248  Peptidase_C1A

ProteinModelPortal

Q6T9Z7   Q26425   C7BWY7   A0A212FHR9   A0A2A4K1J7   A0A2H1VVV9   + More
A0A023J7N9   E2J8A8   I4DJ47   Q6UB44   F8V2Y5   A0A0N0PBH9   S4PHD4   A1DYI6   I4DM20   A0A1E1WFC2   K7J502   U5ET77   A0A194QJW0   Q26636   A0A1W4X5C5   A0A0N9ZTP4   A0A0L7RDJ1   A0A1B0EZ73   A7XH36   A0A1L8DPC9   A0A1L8DPN1   B4KTB9   A0A232F4K0   Q86FS6   C5I793   A0A0M4EVM8   D6X516   A0A1B0CQU1   Q95V59   A0A151JSU9   A0A0C9PTJ9   Q95029-2   Q95029   C6SV44   A0A1W4VQY0   A0A0L0C185   A0A088ARP1   B3NRF0   B4P445   B4JW16   A0A2A3E2D8   V9IF08   A0A0A1XMN3   A0A3B0JLP7   Q7YXL4   B4QEW3   A0A195DTS2   Q7YXL3   B4HQZ0   B4LJE9   E9IEP8   Q28XK9   B4GGA5   R4UMJ7   A0A310SMP9   A0A034W1P8   E2C9C0   O46030   A0A1V1FVL6   A0A067R0U6   V5GVR8   A0A3B0J0I7   A0A182M3E8   B3MHG5   A0A182RYY3   A0A1L8EHK8   A0A195ATU0   A0A1L8EHE3   A0A1D1ZBY8   B4MNQ5   A0A182W1X4   A0A2J7PV32   A0A0U1ZDE6   A0A182J2Y7   A0A336LNC5   A0A182QBB7   A0A158NPU2   T1PFE3   B0WI10   U4U785   N6UN71   A0A1Q3FIJ1   Q17H05   A0A182P7H9   A0A182N3W2   R4FQ93   W8BGA5   T1D4A5   A0A2M3YZP6   A0A2M4BSK6   A0A2M3YZN8   A0A1S4F2V5   J3JY43   A0A023ENZ2  

PDB

1CS8     E-value=1.3196e-91,     Score=857

KEGG

693031    K01365 cathepsin L [EC:3.4.22.15] | (RefSeq) Bcp; fibroinase

PATHWAY

04140    Autophagy - animal - Bombyx mori (domestic silkworm)
04142    Lysosome - Bombyx mori (domestic silkworm)
04145    Phagosome - Bombyx mori (domestic silkworm)

GO

GO:0008234   GO:0016021   GO:0007275   GO:0005764   GO:0030154   GO:0045169   GO:0004197   GO:0005615   GO:0051603   GO:0008233   GO:0006508   GO:0007586   GO:0005856   GO:0016311   GO:0016791   GO:0007166   GO:0016020   GO:0004713   GO:0016491   GO:0015930  

Subcellular location

Lysosome  

SignalP

Position:   1 - 16,         Likelihood:  0.984480

 

 

Length:

341

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

5.29856999999999

Exp number, first 60 AAs:

5.29828

Total prob of N-in:

0.28129

outside

1  -  341

 

 

Pi

213.648956

Theta

180.672913

Tajima's D

0.378926

CLR

0.821101

CSRT

0.478126093695315

Interpretation

Uncertain