SGID Silkworm Genome Informatics Database
Gene
KWMTBOMO13900
Pre Gene Modal
BGIBMGA011590
Annotation
PREDICTED:_uncharacterized_protein_LOC101738187_[Bombyx_mori]
Full name
Ionotropic receptor 25a      
Location in the cell
PlasmaMembrane   Reliability : 3.513
 

Sequence

CDS
ATGACGACGCAGAATATTAATGTTTTATTAATCAACGAAGAAAATAATGCCCTAGCTGAAAAATCCTTTGAAATAGCGAAAGAATATGTGAGACGTAATCCAAGTTTAGGATTAGCGATAGAACCAGTTATTGTCGTTGGAAACAGATCTGACGCCAAAACTTTCCTAGAAAATGTTTGCAGGAAATACAACGATATGCTATCATCGAAAAAAACACCTCACGTTGTCTTGGATTTCACAATGACCGGCGTCGGATCAGAAACTATAAAATCTTTCACCGCTGCATTGGCATTGCCAACGATATCGGGTTCATTCGGACAAACGGGAGATCTACGACAATGGAGGTCACTTAATGCTAACCAAACTAAATTTCTTCTGCAAGTGATGCCTCCTGCAGATATTCTACCTGAATCCATCAGAGCTATTGTTACTAAACAAGATATCACGAATGCCGCAATTATCTTTGACGAGCTTTTTGTTATGGACCATAAATACAAATCTTTACTACAAAATATTCCAACTCGTCATGTTATTACGCCAGTAAAAAGTTTTAACAAAGAAGACATCAAAACTCAATTACGCAGCCTGCGGGAATTGGATATAGTAAATTTCTTCATTGTCGGTAGTTTGAGGACTATCAAAAACGTGCTAGACGCAGCGGATGAAAATCAGTATTTCGGCCGGAAAACGGCCTGGTTCGCGTTCTCGTTAGATAAAGGAGACATTACTTGCGGCTGTAAAGATGCCACAATTGTTTATATGAGACCAACGCCAGACGCAAAGAGCAGAGATCGTTTGGGAAAAATCAAAACCACTTATAGCATGAACGGGGAGCCGGAAATTACATCTGCGTTCTACTTCGACTTGTCTTTGAGGACATTTTTGGCTGTCAAATCCCTACTGGACTCTGGCAAGTGGCCAAATAACATGAAATATATCACATGCGACGATTACGACGGCAAGAATACACCTAACAGGACCTTGGATCTTAAACTGGCTTTTCAAGAGGTGAAAGAGACTCCAACTTATGCTCCGTTTTATATCCCCGGAGACGACCCTATGAATGGAAGAAGCTACATGGAATTTAGTACTGATTTATCGGCAGTTACCGTCAAAGATGGTGCTTCTATAGGTAGTAAGGCCCTAGGAACTTGGAAAGCTGGTCTTAACAGTCCGCTGTCTTTGACTGATTCCGATAACATGAGCGATTATTCCGCTCAACTTGTGTACAGAGTTGTTACAGTCGAGCAACAACCTTTCATAATACGAGACGATAATGCCCCTAAAGGTTTTAAGGGATATTGCATTGACCTAATCGAGGAGATTCGTCAAATCGTTAAGTTTGATTACGAAGTGACTTTATCACCTGACGGTAATTTCGGAACGATGGACGAGAATGGTAACTGGAACGGCATCATAAAGGAATTGATCGAAAAGAGAGCGGATATCGCTCTAACTTCTCTGTCAGTAATGGCTGAGAGGGAAAATGTTGTAGATTTCACAGTGCCTTACTACGATTTGGTGGGTATAACGATAATGATGAAGTTGCCTAGAACACCGACGTCACTGTTTAAATTTTTAACAGTACTGGAAAATGACGTGTGGCTGTCGATACTTGCCGCTTACTTCTTTACCAGCTTTTTAATGTGGGTATTCGACAAATGGAGTCCGTATAGTTACCAGAACAATCGAGAAAAATACAAAGACGACGAGGAAAAACGCGAATTTACGCTTAAAGAATGTCTTTGGTTCTGTATGACGTCACTTACCCCGCAAGGCGGAGGGGAGGCGCCGAAAAACCTATCCGGGAGATTGCTTGCCGCTACTTGGTGGCTGTTCGGTTTTATAATAATAGCATCGTACACCGCGAATTTGGCGGCTTTCCTCACTGTATCTCGTTTGGATACTCCAATAGAATCGTTGGACGATCTATCGAAGCAATACAAAATACAGTACGCTCCATTGAATGGTTCTGCCGCCATGACATATTTCGAAAGGATGGCCGCTATCGAAGTCAGATTTTATGAAATATGGAAAGAAATGAGTTTGAACGACAGTCTAAGTGATGTCGAACGCGCTAAGCTAGCCGTATGGGATTATCCGGTCAGCGATAAATACAGTAAAATGTGGCAGGCCATGAAAGAGGCAGGCCTGCCTAACTCGATAGAGGAAGCCGTGCAAAGGGTGAGGGATTCGAAGAGCTCGAGCGAAGGTTTCGCCTGGCTGGGAGACGCTACGGACGTTAGGTACTATGTCCTGACGAGCTGCGATCTTCAGATGGTCGGAGACGAATTCTCGAGGAAGCCTTACGCTATTGCCGTGCAGCAAGGATCTCCTTTGAAAGACCAGTTTAACAACGCGATTCTGCAGCTACTTAACAGACGTAGACTAGAGAAGCTCAAAGAAAACTGGTGGAACAATAATCCCAAAGCGATGAAATGTGAAAAGCAAGATGACCAGTCCGACGGGATCTCTATTCAGAACATCGGAGGTGTTTTCATAGTTATCTTTATGGGGATAGGACTTGCTTGCATCACTCTGGGCGTCGAGTACTGGTGGTATAAATGGAGGCGACGTCCAATTGTCGGTGACGTCACACAGGTGGAACCAGCTAAATCAACGAGAAATAACATCGGAAATTTCGTCAAAGGCGAAGGATTCACTTTTCGTTCTAGAAACTTCGGTCTCTCCGATTTAAAGCAGAAATTTTAA
Protein
MTTQNINVLLINEENNALAEKSFEIAKEYVRRNPSLGLAIEPVIVVGNRSDAKTFLENVCRKYNDMLSSKKTPHVVLDFTMTGVGSETIKSFTAALALPTISGSFGQTGDLRQWRSLNANQTKFLLQVMPPADILPESIRAIVTKQDITNAAIIFDELFVMDHKYKSLLQNIPTRHVITPVKSFNKEDIKTQLRSLRELDIVNFFIVGSLRTIKNVLDAADENQYFGRKTAWFAFSLDKGDITCGCKDATIVYMRPTPDAKSRDRLGKIKTTYSMNGEPEITSAFYFDLSLRTFLAVKSLLDSGKWPNNMKYITCDDYDGKNTPNRTLDLKLAFQEVKETPTYAPFYIPGDDPMNGRSYMEFSTDLSAVTVKDGASIGSKALGTWKAGLNSPLSLTDSDNMSDYSAQLVYRVVTVEQQPFIIRDDNAPKGFKGYCIDLIEEIRQIVKFDYEVTLSPDGNFGTMDENGNWNGIIKELIEKRADIALTSLSVMAERENVVDFTVPYYDLVGITIMMKLPRTPTSLFKFLTVLENDVWLSILAAYFFTSFLMWVFDKWSPYSYQNNREKYKDDEEKREFTLKECLWFCMTSLTPQGGGEAPKNLSGRLLAATWWLFGFIIIASYTANLAAFLTVSRLDTPIESLDDLSKQYKIQYAPLNGSAAMTYFERMAAIEVRFYEIWKEMSLNDSLSDVERAKLAVWDYPVSDKYSKMWQAMKEAGLPNSIEEAVQRVRDSKSSSEGFAWLGDATDVRYYVLTSCDLQMVGDEFSRKPYAIAVQQGSPLKDQFNNAILQLLNRRRLEKLKENWWNNNPKAMKCEKQDDQSDGISIQNIGGVFIVIFMGIGLACITLGVEYWWYKWRRRPIVGDVTQVEPAKSTRNNIGNFVKGEGFTFRSRNFGLSDLKQKF

Summary

Description
Integral part of various neural sensory systems in the antenna that provide the neural basis for the response to environmental changes in temperature (thermosensation), humidity (hygrosensation) and odor detection (PubMed:21220098, PubMed:27161501, PubMed:27656904). Required for odor-evoked electrophysiological responses in multiple neuron classes in the antenna and is likely to function as part of an olfactory receptor complex with Ir76a and Ir76b (PubMed:21220098). Together with Ir21a and Ir93a, mediates the response of the larval dorsal organ cool cells, a trio of cool-responsive neurons, to cooling and is required for cool avoidance behavior (PubMed:27126188, PubMed:27161501, PubMed:27656904). Required in chordonotal organ neurons for behavioral synchronization to low-amplitude temperature cycles and mediates circadian clock resetting by temperature (PubMed:26580016). Together with Ir40a and Ir93a, mediates the response of the hydrosensory sacculus neurons to changes in relative humidity, and is required for dry detection and humidiy preference behavior (PubMed:27161501, PubMed:27656904).
Subunit
Interacts with nocte.
Similarity
Belongs to the glutamate-gated ion channel (TC 1.A.10.1) family.
Keywords
Behavior   Biological rhythms   Cell membrane   Cell projection   Complete proteome   Glycoprotein   Ion channel   Ion transport   Ligand-gated ion channel   Membrane   Olfaction   Receptor   Reference proteome   Sensory transduction   Signal   Transmembrane   Transmembrane helix   Transport  
Feature
chain  Ionotropic receptor 25a
EMBL
BABH01015551    BABH01015552    GCVX01000133    JAI18097.1    KX084508    ARO76463.1    + More
MH050691    AZT78940.1    MH193958    AZB49404.1    LC017784    BAR64798.1    MG820695    AXF48866.1    KY325450    AQM73611.1    JN836717    AFC91757.2    KY283568    AST36228.1    KY283699    AST36358.1    KX655898    AOG12847.1    RSAL01000081    RVE48574.1    KQ459601    KPI94081.1    AGBW02007799    OWR54543.1    KJ542744    AJD81628.1    NWSH01000246    PCG78050.1    KU702630    AMM70657.1    GENK01000125    JAV45788.1    KF487714    AII01112.1    KT588089    AOE47999.1    KF487724    AII01122.1    KX252767    APY22696.1    KX585393    ARO70286.1    ODYU01002206    SOQ39397.1    JTDY01000213    KOB78263.1    KP296780    AJO62244.1    KQ971311    EEZ98989.2    KY817099    AVH87315.1    JXUM01110311    JXUM01110312    JXUM01110313    JXUM01110314    JXUM01110315    KQ565401    KXJ71028.1    CH477615    EAT38264.1    AXCN02001867    GFXV01003146    MBW14951.1    ABLF02036596    MH230261    AXY87920.1    GGMS01010745    MBY79948.1    MH230262    AXY87921.1    APCN01005276    AAAB01008980    EAA13931.5    AXCM01000163    KT381529    ALR72535.1    ATLV01020673    KE525304    KFB45509.1    AJWK01006611    GGOB01000051    MCH29447.1    MG766215    AVN97885.1    GGMR01008132    MBY20751.1    CVRI01000001    CRK86500.1    DS235028    EEB10703.1    CH916372    EDV99095.1    GAMC01005235    JAC01321.1    CH940661    EDW71306.2    FX985960    BBD43495.1    GAKP01001202    JAC57750.1    CH933807    EDW13247.2    GDHF01009934    JAI42380.1    CH963920    EDW77591.2    KT381711    PYGN01001004    AMA98202.1    PSN38557.1    GDHC01017435    JAQ01194.1    KU291867    AOE48117.1    CH902620    EDV31713.2    CH479180    EDW28806.1    HQ600588    AE014134    ADU79032.1    BT099588    ACU43550.1    AAF50976.2    AGB92587.1    AGB92586.1    CH379060    EAL33639.3    CM002910    KMY88125.1    KMY88124.1    CM000157    EDW87678.2    CM000361    EDX03748.1    OUUW01000004    SPP79275.1    CH480820    EDW54275.1    GABX01000048    JAA74471.1    KB632161    ERL89264.1    JX263304    AFP89966.1    CP012523    ALC38173.1    JRES01000753    KNC28739.1    KJ702119    AID61273.1    CH954177    EDV57772.2    KK853313    KDR08634.1    ACPB03014189    KU523597    APZ81419.1    APGK01043536    APGK01043537    KB741015    ENN75289.1   
Pfam
PF10613   Lig_chan-Glu_bd        + More
PF00060   Lig_chan
PF01094   ANF_receptor
PF08445   FR47
Interpro
IPR001320   Iontro_rcpt        + More
IPR019594   Glu/Gly-bd       
IPR001508   Iono_rcpt_met       
IPR028082   Peripla_BP_I       
IPR000182   GNAT_dom       
IPR001828   ANF_lig-bd_rcpt       
IPR016181   Acyl_CoA_acyltransferase       
IPR013653   FR47       
SUPFAM
SSF53822   SSF53822        + More
SSF55729   SSF55729       
PDB
5KUF     E-value=2.84655e-67,     Score=652

Ontologies

Topology

Subcellular location
  
Cell membrane   Low levels detected in the axon segment adjacent to the perikaryon in some sensory neurons of the antenna but not detected along axons as they enter the brain or at synapses within antennal lobe glomeruli. In coeloconic neurons, prominently expressed both in the perikaryon and in the distal tip of the dendrite which corresponds to the ciliated outer dendritic segment innervating the sensory hair. Relatively low levels detected in inner dendrites (PubMed:19135896). Detected in dendritic bulbs of the dorsal organ cool cells (PubMed:27126188).   With evidence from 4 publications.
Cell projection   Low levels detected in the axon segment adjacent to the perikaryon in some sensory neurons of the antenna but not detected along axons as they enter the brain or at synapses within antennal lobe glomeruli. In coeloconic neurons, prominently expressed both in the perikaryon and in the distal tip of the dendrite which corresponds to the ciliated outer dendritic segment innervating the sensory hair. Relatively low levels detected in inner dendrites (PubMed:19135896). Detected in dendritic bulbs of the dorsal organ cool cells (PubMed:27126188).   With evidence from 4 publications.
Axon   Low levels detected in the axon segment adjacent to the perikaryon in some sensory neurons of the antenna but not detected along axons as they enter the brain or at synapses within antennal lobe glomeruli. In coeloconic neurons, prominently expressed both in the perikaryon and in the distal tip of the dendrite which corresponds to the ciliated outer dendritic segment innervating the sensory hair. Relatively low levels detected in inner dendrites (PubMed:19135896). Detected in dendritic bulbs of the dorsal organ cool cells (PubMed:27126188).   With evidence from 4 publications.
Dendrite   Low levels detected in the axon segment adjacent to the perikaryon in some sensory neurons of the antenna but not detected along axons as they enter the brain or at synapses within antennal lobe glomeruli. In coeloconic neurons, prominently expressed both in the perikaryon and in the distal tip of the dendrite which corresponds to the ciliated outer dendritic segment innervating the sensory hair. Relatively low levels detected in inner dendrites (PubMed:19135896). Detected in dendritic bulbs of the dorsal organ cool cells (PubMed:27126188).   With evidence from 4 publications.
Perikaryon   Low levels detected in the axon segment adjacent to the perikaryon in some sensory neurons of the antenna but not detected along axons as they enter the brain or at synapses within antennal lobe glomeruli. In coeloconic neurons, prominently expressed both in the perikaryon and in the distal tip of the dendrite which corresponds to the ciliated outer dendritic segment innervating the sensory hair. Relatively low levels detected in inner dendrites (PubMed:19135896). Detected in dendritic bulbs of the dorsal organ cool cells (PubMed:27126188).   With evidence from 4 publications.
Cilium   Low levels detected in the axon segment adjacent to the perikaryon in some sensory neurons of the antenna but not detected along axons as they enter the brain or at synapses within antennal lobe glomeruli. In coeloconic neurons, prominently expressed both in the perikaryon and in the distal tip of the dendrite which corresponds to the ciliated outer dendritic segment innervating the sensory hair. Relatively low levels detected in inner dendrites (PubMed:19135896). Detected in dendritic bulbs of the dorsal organ cool cells (PubMed:27126188).   With evidence from 4 publications.
Length:
903
Number of predicted TMHs:
3
Exp number of AAs in TMHs:
64.90696
Exp number, first 60 AAs:
0.00129
Total prob of N-in:
0.03315
outside
1  -  533
TMhelix
534  -  552
inside
553  -  608
TMhelix
609  -  631
outside
632  -  831
TMhelix
832  -  854
inside
855  -  903
 
 

Population Genetic Test Statistics

Pi
283.942141
Theta
186.501098
Tajima's D
0.339548
CLR
0.648541
CSRT
0.468126593670317
Interpretation
Uncertain
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