Gene

KWMTBOMO13744  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA011521

Annotation

asparagine_synthetase_[Bombyx_mori]

Location

Bomo_Chr23(+):8172295-8174047      View in EpiBrowser

Full name

Asparagine synthetase [glutamine-hydrolyzing]      

Alternative Name

Glutamine-dependent asparagine synthetase

Location in the cell

Cytoplasmic   Reliability : 1.86 Mitochondrial   Reliability : 1.224

CDS

ATGTGCGGTATTTGGGCAACATTCGGTGTAACTGGTGGCCTCACTCCTACCTGCGTAAAATGTTTTTCAAACATTCGCCACCGAGGTCCGGACGCGTGGCGAATTGAGCAAGATGCTAGAGAGCCGTTGGCTATTCTCGGATTTCAACGTCTCGCTATCGTGGACGGTCGGCACGGTATGCAACCGATGCGCCTACACTGTTACCCTCGCACTACGTTAATCTGTAATGGTGAGATTTACAACTGTAAACGCCTACAGAAACAGCATGAGTTTCAATATGAAACAAACTGTGATGTCGAAGCCATCATACATTTGTATCAAAGATTCGGCATAGAAGAAGCCGTCACTAACCTTGACGGGGTTTTTTCGTTTTGCCTTGTCGACGGTGAGAAGAACAAAGTTTATATCGCCCGTGATCCTTATGGCGTTAGACCTCTGTTTAGTTTTCATGACCCTGAAAACGATGTTCTCGCTATTTGTTCTGAAGCTAAAGGCTTGCTCGGCTTAAAGCAAAATGGCAGCCCAAACTCGACACTCGGTCAATTCTCTCCGGGTAACTTACAAGTGTGGAGTATACTTGAAAGTGGTAAAGTCAAACTTGATTACACACATCAATATTTCACACCTGGGAAGGCACCCAAATTTGAGCCTTACGTACCGGAAAATAAATTAATCGATATGAACGTCAATGAAAAGATTGCGTATTTATTGGAGGCCGCTTGCCGAAAACGACTTATGTCCGATCGACGAATAGGCTGTTTACTTAGTGGTGGATTAGATTCCTCGTTAATCACCGCCATTGTAGTGAAACTTGCAAAGGAATACAAATTGCCTTACAAAATACAAACTTTCGCAATCGGGATGGGCGATTCTCCAGATCTTGCAGCCGCCAGAACCGTGGCGGATTATTTAGGAACGGAACATCACGAAGTTCAATTCGATGAAAACGATATAAGAAAAGATCTGGATAACGTTATTTACCATTTAGAATCTTATGATATAACAACTATTCGAGCGAGTTTGCCAATGTACCTTTTGTCAAAGTATATTAAAGAAAAAACAGACACAACAGTGGTATTTAGCGGAGAAGGAGCCGATGAACTAGCGCAAGGCTATATCTACTTCAGAGACGCACCGTCTGAAAAGGACGCACATAAAGAGAGCGTACGTTTGCTTTCTGATATTTATCTCTATGATGGACTGCGAGCAGATAGAACGACGAGTGCCTTTAGTTTAGAATTACGAGTACCGTTTTTAGATATCCAATTCACTCATCACTATCTTTCAATACCTCCTAAACTACGACAACCCCAAAACGGCGTTGAGAAACATCTGTTAAGAAGTAGTTTTGCAAAAAGTGGATTATTGCCAGACTGTGTGCTGTGGCGACACAAAGAAGCATTTAGTGATGGTGTGTCTTCAGTTAAAAAATCGTTGTTCTCGGTCATAAAAGAGATCACAACTGAAAGATTACAAGAAGACGATATACAATATCCAGGACTGCAGCCGAAAACGAATGAATCCAAATATTACCGCTACGTATTTGAGAAATCATTTCCAGGACAACATAATTTTACTCCGTACTATTGGATGCCAAAATGGGTTCAGGTTTCAGACCCTTCGGCAAGGTTTATTAAACATTATGCGGCAAAATGA

Protein

MCGIWATFGVTGGLTPTCVKCFSNIRHRGPDAWRIEQDAREPLAILGFQRLAIVDGRHGMQPMRLHCYPRTTLICNGEIYNCKRLQKQHEFQYETNCDVEAIIHLYQRFGIEEAVTNLDGVFSFCLVDGEKNKVYIARDPYGVRPLFSFHDPENDVLAICSEAKGLLGLKQNGSPNSTLGQFSPGNLQVWSILESGKVKLDYTHQYFTPGKAPKFEPYVPENKLIDMNVNEKIAYLLEAACRKRLMSDRRIGCLLSGGLDSSLITAIVVKLAKEYKLPYKIQTFAIGMGDSPDLAAARTVADYLGTEHHEVQFDENDIRKDLDNVIYHLESYDITTIRASLPMYLLSKYIKEKTDTTVVFSGEGADELAQGYIYFRDAPSEKDAHKESVRLLSDIYLYDGLRADRTTSAFSLELRVPFLDIQFTHHYLSIPPKLRQPQNGVEKHLLRSSFAKSGLLPDCVLWRHKEAFSDGVSSVKKSLFSVIKEITTERLQEDDIQYPGLQPKTNESKYYRYVFEKSFPGQHNFTPYYWMPKWVQVSDPSARFIKHYAAK

Catalytic Activity

ATP + H2O + L-aspartate + L-glutamine = AMP + diphosphate + H(+) + L-asparagine + L-glutamate

Cofactor

Mn(2+)

Miscellaneous

The temperature-sensitive mutation affecting G1 progression results from a single amino acid substitution.

Similarity

Belongs to the glycosyltransferase 2 family. GalNAc-T subfamily.

Keywords

Acetylation   Amino-acid biosynthesis   Asparagine biosynthesis   ATP-binding   Glutamine amidotransferase   Ligase   Nucleotide-binding   Phosphoprotein   Complete proteome   Reference proteome  

Feature

chain  Asparagine synthetase [glutamine-hydrolyzing]

Uniprot

H9JPR6   Q2L9S5   A0A194RR70   A0A194PSC8   S4PMX8   A0A3G1T167   + More
A0A0L7KX40   A0A2H1V6T3   A0A212FFL1   A0A151X8W9   F4X3F1   E9IBX6   A0A195CJ11   A0A158NKV4   A0A195BLW4   A0A067RI79   A0A195DG80   A0A0J7L123   A0A026VVC4   A0A2J7QC70   A0A2P8XMC8   E2BXP2   E2AJW0   A0A2A3EAF7   A0A1B6MEB6   K7ILZ5   A0A232F6W7   J3JXG4   A0A0A9Y0E6   A0A195FCU3   N6U6K6   A0A1W4XK19   A0A1Y1M6J0   A0A139WI23   A0A224XMM5   A0A0V0G3T6   A0A0P4VKA9   E0VXU0   T1IAZ5   A0A2H8TQ21   A0A2R7W4X3   A0A0L7RFS1   A0A2S2N865   A0A0T6AV08   J9JMX7   A0A0C9R706   T1JD29   A0A1S3INM5   V4ASQ3   A0A2T7PBS1   A0A3S0ZWP2   A0A2D4LJS2   A0A146M7S5   W5MYW5   A0A1W7RJ78   J3SE12   T1E786   A0A098M1N3   A0A0B8RZN1   U3EUA1   R7TGJ1   A0A3S1A575   A0A1W0WEP1   A0A210PVE5   K1PWQ4   M7B7L1   K7F8X7   A0A3Q3GVD8   G3I3J1   P19891   A0A3L7IKG1   A0A1S3ICI2   A0A0B6Z4D5   A0A3N0Y0M4   H0VFI5   I3KUD9   A0A2D0S270   P49088   W5U727   A0A286XA60   A0A1A8IH31   B5X2U4   A0A340WVH0   A0A1U7Q2I5   P17714   A0A2U4AR81   A3KQK2   A0A383YPQ6   Q7ZTW9   A0A2I4CL75   V9KLU5   H2LY62   A0A3P9MBJ8   M4AR02   Q61024   A0A1A8KC68   A0A1A8BTC2   A0A1A8VGN5   A0A091T1K5   A0A341D7L1  

EC Number

6.3.5.4

Pubmed

19121390    26354079    23622113    26227816    22118469    21719571    + More
21282665    21347285    24845553    24508170    30249741    29403074    20798317    20075255    28648823    22516182    25401762    23537049    28004739    18362917    19820115    27129103    20566863    26383154    23254933    26823975    26358130    23025625    23758969    25727380    25476704    23915248    28812685    22992520    23624526    17381049    21804562    23929341    2477309    29704459    21993624    25186727    7818476    15489334    23127152    20433749    1972978    23594743    24402279    17554307    23542700    16141072    21183079    24139043   

EMBL

BABH01015193    DQ343760    ABC69171.1    KQ459833    KPJ19805.1    KQ459601    + More
KPI94030.1    GAIX01003305    JAA89255.1    MG846873    AXY94725.1    JTDY01004893    KOB67626.1    ODYU01000963    SOQ36509.1    AGBW02008806    OWR52518.1    KQ982409    KYQ56826.1    GL888613    EGI58979.1    GL762137    EFZ22002.1    KQ977649    KYN00731.1    ADTU01018949    KQ976439    KYM86719.1    KK852665    KDR18971.1    KQ980886    KYN11847.1    LBMM01001428    KMQ96336.1    KK107796    QOIP01000013    EZA47738.1    RLU15169.1    NEVH01016291    PNF26186.1    PYGN01001729    PSN33161.1    GL451281    EFN79560.1    GL440100    EFN66276.1    KZ288311    PBC28454.1    GEBQ01005699    JAT34278.1    NNAY01000772    OXU26596.1    BT127933    AEE62895.1    GBHO01020589    JAG23015.1    KQ981693    KYN37864.1    APGK01040817    KB740984    KB631899    ENN76286.1    ERL86933.1    GEZM01042355    GEZM01042354    GEZM01042353    GEZM01042352    JAV79915.1    KQ971343    KYB27475.1    GFTR01007177    JAW09249.1    GECL01003425    JAP02699.1    GDKW01001849    JAI54746.1    DS235840    EEB18196.1    ACPB03000844    GFXV01003543    MBW15348.1    KK854219    PTY13265.1    KQ414601    KOC69684.1    GGMR01000659    MBY13278.1    LJIG01022749    KRT78921.1    ABLF02023962    ABLF02023963    GBYB01012114    JAG81881.1    JH432094    KB201370    ESO96771.1    PZQS01000005    PVD30867.1    RQTK01000080    RUS88523.1    IACM01031018    LAB21307.1    GDHC01002798    JAQ15831.1    AHAT01004624    GDAY02000209    JAV51204.1    JU173940    GBEX01000289    AFJ49466.1    JAI14271.1    GAAZ01000221    GBKC01000298    GBKD01000194    JAA97722.1    JAG45772.1    JAG47424.1    GBSI01000280    JAC96215.1    GBSH01000351    JAG68673.1    GAEP01000173    GBEW01000100    JAB54648.1    JAI10265.1    AMQN01013089    KB309954    ELT92908.1    RQTK01000021    RUS90971.1    MTYJ01000119    OQV13659.1    NEDP02005461    OWF40463.1    JH815782    EKC26133.1    KB534798    EMP33931.1    AGCU01029125    AGCU01029126    AGCU01029127    AGCU01029128    AGCU01029129    AGCU01029130    JH001188    KE663634    EGW08354.1    ERE91184.1    M27838    X12950    RAZU01000039    RLQ78470.1    HACG01016377    CEK63242.1    RJVU01057109    ROK35680.1    AAKN02016952    AAKN02016953    AERX01064707    AERX01064708    AERX01064709    AERX01064710    U07202    U07201    BC081719    JT406840    AHH37746.1    HAED01010057    SBQ96269.1    BT045363    BT059666    ACI33625.1    ACN11379.1    X52130    BX842688    BC052127    AAH52127.1    JW866458    AFO98975.1    U38940    AK076207    AK167524    BC005552    HAEE01009209    SBR29259.1    HADZ01005829    HAEA01009993    SBP69770.1    HADY01014562    HAEJ01018584    SBS59041.1    KK940410    KFQ50258.1   

Proteomes

UP000005204    UP000053240    UP000053268    UP000037510    UP000007151    UP000075809    + More
UP000007755    UP000078542    UP000005205    UP000078540    UP000027135    UP000078492    UP000036403    UP000053097    UP000279307    UP000235965    UP000245037    UP000008237    UP000000311    UP000242457    UP000002358    UP000215335    UP000078541    UP000019118    UP000030742    UP000192223    UP000007266    UP000009046    UP000015103    UP000053825    UP000007819    UP000085678    UP000030746    UP000245119    UP000271974    UP000018468    UP000014760    UP000242188    UP000005408    UP000031443    UP000007267    UP000261660    UP000001075    UP000030759    UP000273346    UP000005447    UP000005207    UP000221080    UP000002494    UP000087266    UP000265300    UP000189706    UP000245320    UP000000437    UP000261681    UP000192220    UP000001038    UP000265180    UP000002852    UP000000589    UP000252040   

PRIDE

H9JPR6    Q2L9S5    P19891    P49088    Q61024   

Pfam

PF13537   GATase_7        + More
PF00733   Asn_synthase
PF00929   RNase_T
PF00535   Glycos_transf_2
PF00652   Ricin_B_lectin
PF15072   DUF4539

Interpro

IPR017932   GATase_2_dom        + More
IPR029055   Ntn_hydrolases_N       
IPR001962   Asn_synthase       
IPR014729   Rossmann-like_a/b/a_fold       
IPR006426   Asn_synth_AEB       
IPR033738   AsnB_N       
IPR012337   RNaseH-like_sf       
IPR013520   Exonuclease_RNaseT/DNA_pol3       
IPR022894   Oligoribonuclease       
IPR036397   RNaseH_sf       
IPR001173   Glyco_trans_2-like       
IPR029044   Nucleotide-diphossugar_trans       
IPR035992   Ricin_B-like_lectins       
IPR000772   Ricin_B_lectin       
IPR028045   DUF4539       

SUPFAM

SSF56235   SSF56235        + More
SSF53098   SSF53098       
SSF53448   SSF53448       
SSF50370   SSF50370       

Gene 3D

3.40.50.620     3.60.20.10     3.30.420.10     3.90.550.10    

CDD

cd00712  AsnB
cd01991  Asn_Synthase_B_C
cd06135  Orn
cd00161  RICIN

ProteinModelPortal

H9JPR6   Q2L9S5   A0A194RR70   A0A194PSC8   S4PMX8   A0A3G1T167   + More
A0A0L7KX40   A0A2H1V6T3   A0A212FFL1   A0A151X8W9   F4X3F1   E9IBX6   A0A195CJ11   A0A158NKV4   A0A195BLW4   A0A067RI79   A0A195DG80   A0A0J7L123   A0A026VVC4   A0A2J7QC70   A0A2P8XMC8   E2BXP2   E2AJW0   A0A2A3EAF7   A0A1B6MEB6   K7ILZ5   A0A232F6W7   J3JXG4   A0A0A9Y0E6   A0A195FCU3   N6U6K6   A0A1W4XK19   A0A1Y1M6J0   A0A139WI23   A0A224XMM5   A0A0V0G3T6   A0A0P4VKA9   E0VXU0   T1IAZ5   A0A2H8TQ21   A0A2R7W4X3   A0A0L7RFS1   A0A2S2N865   A0A0T6AV08   J9JMX7   A0A0C9R706   T1JD29   A0A1S3INM5   V4ASQ3   A0A2T7PBS1   A0A3S0ZWP2   A0A2D4LJS2   A0A146M7S5   W5MYW5   A0A1W7RJ78   J3SE12   T1E786   A0A098M1N3   A0A0B8RZN1   U3EUA1   R7TGJ1   A0A3S1A575   A0A1W0WEP1   A0A210PVE5   K1PWQ4   M7B7L1   K7F8X7   A0A3Q3GVD8   G3I3J1   P19891   A0A3L7IKG1   A0A1S3ICI2   A0A0B6Z4D5   A0A3N0Y0M4   H0VFI5   I3KUD9   A0A2D0S270   P49088   W5U727   A0A286XA60   A0A1A8IH31   B5X2U4   A0A340WVH0   A0A1U7Q2I5   P17714   A0A2U4AR81   A3KQK2   A0A383YPQ6   Q7ZTW9   A0A2I4CL75   V9KLU5   H2LY62   A0A3P9MBJ8   M4AR02   Q61024   A0A1A8KC68   A0A1A8BTC2   A0A1A8VGN5   A0A091T1K5   A0A341D7L1  

PDB

1CT9     E-value=4.15162e-73,     Score=700

KEGG

692995    K01953 asparagine synthase (glutamine-hydrolysing) [EC:6.3.5.4] | (RefSeq) asparagine synthetase

PATHWAY

00250    Alanine, aspartate and glutamate metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01230    Biosynthesis of amino acids - Bombyx mori (domestic silkworm)

GO

GO:0004066   GO:0006529   GO:0005524   GO:0006541   GO:0000175   GO:0003676   GO:0005794   GO:0016021   GO:0006486   GO:0030246   GO:0016757   GO:0005829   GO:0045931   GO:0043066   GO:0042149   GO:0070981   GO:0032354   GO:0031427   GO:0009416   GO:0001889   GO:0031667   GO:0009636   GO:0043200   GO:0009612   GO:0006520   GO:0048037   GO:0032870   GO:0042803   GO:0031625   GO:0005515   GO:0000287   GO:0016620   GO:0016787   GO:0016491   GO:0015930  

Length:

551

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.419500000000001

Exp number, first 60 AAs:

0.12754

Total prob of N-in:

0.01216

outside

1  -  551

 

 

Pi

155.579539

Theta

186.764735

Tajima's D

-0.637383

CLR

141.874101

CSRT

0.213239338033098

Interpretation

Possibly Positive selection