Gene

KWMTBOMO13699

Pre Gene Modal

BGIBMGA011091

Annotation

PREDICTED:_lysocardiolipin_acyltransferase_1-like_[Bombyx_mori]

Location

Bomo_Chr23(+):7214005-7220324      View in EpiBrowser

Full name

Lysocardiolipin acyltransferase 1      

Alternative Name

Acyl-CoA:lysocardiolipin acyltransferase 1
1-acylglycerol-3-phosphate O-acyltransferase 8

Location in the cell

PlasmaMembrane   Reliability : 1.799

CDS

ATGATAATGAATCATCGAACCAGGGTTGATTGGAATTATGTATGGATCGCTTTATATCACGCTACGCAAAACCCAAATGAAGATTGTCGTGTGTGTAAAGAGCAATTAAATGGTTTAGAGGAACCTGGAAGTGTTTTCGATATTGGAGGGAAATCAAAAATCAAAATCGTTTTAAAAGACGGAATCAAAAACATTCCCGCAATAGGGTGGATAATGCAACTAAACTTCTTTTTGTACGTGAAAAGAAATTGGCAAGAAGATAACAATAATTTGAATCAGTTTGTAGACTACTATAAAAGTTTGAAATACGATTATCGCTTGGTTTTGTTCCCCGAGGGTACGGATTTGAGCGAGAGCAATAAAATTCGAAGCGACAGTTTTGCTGTTGCTCACAATTTGCCTAAATATGACTTTGTCTTACACCCTCGTTTGACTGGATGGTCAGCGCTGTGCTCACGGCTTCGAGGAACCGGGCTCGCTTCGGTATACGATGTCACCCTAGCCTACGATAACCCCGCACAGACCGAACTCGATCTGGCCAAAGGAAACATACCTAAAAATGTGTACTTTCATTTTAAAAGGTATTCGATAGATAAAATACCATACAATGAGGAAGATTTAAAGAAATGGTTAAACGATAGATGGGGTGAAAAGGAAATTAGTCTTCGTAAATTTCATAAAGAAGGTAATTTCATAGATTTTGAAACAACGACACCTCCCGCATACAGGTCCCCGCGTTCGATGATCGTAGCTAAAGCAGGCTTTACATTTTGGACAATTGTAAATTTATTATTTACCTACGCTATATATTGTAGTGTAATGTTTCAATTTTGGGTTTTGTACCATAGTGTATTATTCGTGATAGTAACATGTTATTTAGGTGGTTTTCAGAACATTCAATACAGGCTACTCAAAAATGAAACACCATAA

Protein

MIMNHRTRVDWNYVWIALYHATQNPNEDCRVCKEQLNGLEEPGSVFDIGGKSKIKIVLKDGIKNIPAIGWIMQLNFFLYVKRNWQEDNNNLNQFVDYYKSLKYDYRLVLFPEGTDLSESNKIRSDSFAVAHNLPKYDFVLHPRLTGWSALCSRLRGTGLASVYDVTLAYDNPAQTELDLAKGNIPKNVYFHFKRYSIDKIPYNEEDLKKWLNDRWGEKEISLRKFHKEGNFIDFETTTPPAYRSPRSMIVAKAGFTFWTIVNLLFTYAIYCSVMFQFWVLYHSVLFVIVTCYLGGFQNIQYRLLKNETP

Description

Acyl-CoA:lysocardiolipin acyltransferase. Possesses both lysophosphatidylinositol acyltransferase (LPIAT) and lysophosphatidylglycerol acyltransferase (LPGAT) activities. Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors. Acts as a remodeling enzyme for cardiolipin, a major membrane polyglycerophospholipid. Converts lysophosphatidic acid (LPA) into phosphatidic acid (PA) with a relatively low activity. Required for establishment of the hematopoietic and endothelial lineages.

Catalytic Activity

a 1-acyl-sn-glycero-3-phosphate + an acyl-CoA = a 1,2-diacyl-sn-glycero-3-phosphate + CoA

Biophysicochemical Properties

39 uM for arachidonoyl-CoA (20:4) for LPIAT activity
289 uM for oleoyl-CoA (18:1) for LPIAT activity
134 uM for linoleoyl-CoA (18:2) for LPIAT activity
34 uM for stearoyl-CoA (18:0) for LPIAT activity
53 uM for palmitoyl-CoA (16:0) for LPIAT activity
54 uM for oleoyl-CoA (18:1) for LPGAT activity
36 uM for linoleoyl-CoA (18:2) for LPGAT activity
70 uM for stearoyl-CoA (18:0) for LPGAT activity
34 uM for palmitoyl-CoA (16:0) for LPGAT activity

Similarity

Belongs to the 1-acyl-sn-glycerol-3-phosphate acyltransferase family.

Keywords

Acetylation   Acyltransferase   Complete proteome   Developmental protein   Endoplasmic reticulum   Glycoprotein   Lipid biosynthesis   Lipid metabolism   Membrane   Phospholipid biosynthesis   Phospholipid metabolism   Reference proteome   Transferase   Transmembrane   Transmembrane helix   Alternative splicing   Polymorphism  

Feature

chain  Lysocardiolipin acyltransferase 1
splice variant  In isoform 3.
sequence variant  In dbSNP:rs12471868.

Uniprot

H9JNI7   A0A2A4K917   A0A2H1X306   A0A212FFS4   A0A437BUG7   A0A2A4KAE8   + More
A0A0L7KVQ6   A0A194QP89   A0A026WL94   A0A195E1J5   E2B044   A0A1W4XDW2   K7IWB4   F4X0W7   A0A0L7RK49   A0A154P894   A0A232FK54   A0A310SMU1   A0A151JV26   A0A087ZPW6   A0A2A3EJH7   A0A195CP85   A0A151HYW1   E9IW27   A0A1B6CQK0   J9JM54   A0A139WGY0   A0A139WGJ9   A0A0M8ZR39   A0A3Q3AHD5   A0A3Q3AHE9   A0A444SRI1   F6Z1A0   A0A218ULG5   A0A2R8MWP2   G5C2X2   A0A151WGS7   A0A091EK02   A0A091CX85   A0A1L8G137   M3XZI5   B0V2Q7   Q3UN02   A0A3B3DKB6   A0A091IA28   V9IH79   L5KT26   A0A402F096   J9NVZ5   G3T046   A0A1D2MM63   K9IXI5   U6DRD8   F6QAZ7   B4F6M8   I3M4Q4   H9F706   A0A151P408   A0A2R8MKZ9   A0A3B3DIV3   A0A2R8ZVQ4   A0A2Y9K6N5   A0A2U3XP59   G1RZV1   A0A3B2WDB3   U3K9X0   A0A3Q7UWD0   A0A2I2Z6V6   Q6UWP7-3   A0A2K5S5T8   A0A1U7RPJ9   A0A1W7RG27   A0A2I3HWC9   A0A1S3EZ85   A0A093GZX9   A0A2K5E023   D3ZFF4   B4DYR5   A0A315UYR5   A0A2U3WL33   A0A2K6DV35   A0A2K5W165   F6QWR0   A0A2I2YN45   Q6UWP7   A0A2Y9HMX2   A0A0D9RFE8   A0A2K5E028   A0A3Q4I762   A0A091H8Y3   A0A2K5K8L4   K7EDA5   A0A1U7Q9A2   G3GW77   A0A091SZY6   A0A3S2MEK2   A0A0Q3LY38   A0A2K6DV17   A0A2K5W176  

Pubmed

19121390    22118469    26227816    26354079    24508170    30249741    + More
20798317    20075255    21719571    28648823    21282665    18362917    19820115    19892987    21993625    27762356    12040188    15489334    21183079    19468303    16141072    15152008    16620771    17675553    29451363    23258410    16341006    27289101    20431018    25319552    22293439    22722832    22398555    12975309    14702039    15815621    19075029    19608861    21406692    26358130    15057822    15632090    29703783    17431167    22002653    25186727    18464734    21804562   

EMBL

BABH01019598    NWSH01000020    PCG80711.1    ODYU01013012    SOQ59616.1    AGBW02008774    + More
OWR52573.1    RSAL01000008    RVE54030.1    PCG80710.1    JTDY01005148    KOB67323.1    KQ461195    KPJ06790.1    KK107154    QOIP01000001    EZA56810.1    RLU27164.1    KQ979814    KYN18971.1    GL444387    EFN60942.1    GL888503    EGI59909.1    KQ414579    KOC71111.1    KQ434824    KZC07340.1    NNAY01000110    OXU30879.1    KQ760832    OAD58884.1    KQ981713    KYN37383.1    KZ288238    PBC31336.1    KQ977565    KYN01919.1    KQ976712    KYM76977.1    GL766449    EFZ15195.1    GEDC01021546    JAS15752.1    ABLF02036165    ABLF02036173    KQ971344    KYB27111.1    KYB27110.1    KQ435966    KOX67923.1    SAUD01025821    RXG56005.1    MUZQ01000248    OWK54202.1    JH173133    EHB15883.1    KQ983152    KYQ47018.1    KK718445    KFO56699.1    KN123775    KFO23312.1    CM004475    OCT77518.1    AEYP01092777    AEYP01092778    AEYP01092779    AEYP01092780    AEYP01092781    AEYP01092782    AEYP01092783    AEYP01092784    BC147499    BC150884    BC158076    BC158079    CH466537    AAI47500.1    AAI50885.1    AAI58077.1    EDL38407.1    AC112949    AK144580    KL217566    KFO96620.1    JR047430    AEY60415.1    KB030580    ELK14101.1    BDOT01000073    GCF49925.1    AAEX03010818    AAEX03010819    AAEX03010820    AAEX03010821    LJIJ01000894    ODM93894.1    GABZ01006594    JAA46931.1    HAAF01012620    CCP84443.1    AAMC01085348    AAMC01085349    AAMC01085350    AAMC01085351    AAMC01085352    AAMC01085353    AAMC01085354    AAMC01085355    AAMC01085356    AAMC01085357    BC167939    AAI67939.1    AGTP01024972    AGTP01024973    AGTP01024974    AGTP01024975    JU326659    AFE70415.1    AKHW03001146    KYO43485.1    AJFE02095327    AJFE02095328    AJFE02095329    AJFE02095330    AJFE02095331    ADFV01011499    ADFV01011500    ADFV01011501    ADFV01011502    ADFV01011503    CT025726    AGTO01020503    CABD030013617    CABD030013618    CABD030013619    CABD030013620    AY358702    AK095284    AC073255    AC132154    BC146817    GDAY02001330    JAV50097.1    KL205627    KFV72357.1    AABR07063254    AABR07063255    AABR07063256    AABR07063257    CH473947    EDM02860.1    AK302562    BAG63827.1    NHOQ01002481    PWA16555.1    AQIA01018653    AQIA01018654    AQIA01018655    AQIA01018656    JSUE03010496    JSUE03010497    JSUE03010498    CM001265    EHH22015.1    AQIB01034473    AQIB01034474    AQIB01034475    AQIB01034476    AQIB01034477    AQIB01034478    AQIB01034479    AQIB01034480    KL525764    KFO91120.1    JH000049    EGV98319.1    KK939047    KFQ49267.1    CM012458    RVE57409.1    LMAW01002934    KQK75472.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000283053    UP000037510    UP000053240    + More
UP000053097    UP000279307    UP000078492    UP000000311    UP000192223    UP000002358    UP000007755    UP000053825    UP000076502    UP000215335    UP000078541    UP000005203    UP000242457    UP000078542    UP000078540    UP000007819    UP000007266    UP000053105    UP000264800    UP000288706    UP000002281    UP000197619    UP000008225    UP000006813    UP000075809    UP000052976    UP000028990    UP000186698    UP000000715    UP000000589    UP000261560    UP000054308    UP000010552    UP000288954    UP000002254    UP000007646    UP000094527    UP000008143    UP000005215    UP000050525    UP000240080    UP000248482    UP000245341    UP000001073    UP000016665    UP000286640    UP000001519    UP000005640    UP000233040    UP000189705    UP000081671    UP000053584    UP000233020    UP000002494    UP000245340    UP000233120    UP000233100    UP000006718    UP000248481    UP000029965    UP000261580    UP000233080    UP000002279    UP000189706    UP000001075    UP000051836   

PRIDE

H9JNI7    Q3UN02    Q6UWP7    D3ZFF4    B4DYR5    G3GW77    + More

Pfam

PF01553   Acyltransferase        + More
PF16076   Acyltransf_C
PF00083   Sugar_tr
PF07690   MFS_1

Interpro

IPR002123   Plipid/glycerol_acylTrfase        + More
IPR032098   Acyltransf_C       
IPR005828   MFS_sugar_transport-like       
IPR036259   MFS_trans_sf       
IPR020846   MFS_dom       
IPR005829   Sugar_transporter_CS       
IPR011701   MFS       
IPR011990   TPR-like_helical_dom_sf       

SUPFAM

SSF103473   SSF103473        + More
SSF48452   SSF48452       

Gene 3D

1.25.40.10    

CDD

cd06174  MFS

ProteinModelPortal

H9JNI7   A0A2A4K917   A0A2H1X306   A0A212FFS4   A0A437BUG7   A0A2A4KAE8   + More
A0A0L7KVQ6   A0A194QP89   A0A026WL94   A0A195E1J5   E2B044   A0A1W4XDW2   K7IWB4   F4X0W7   A0A0L7RK49   A0A154P894   A0A232FK54   A0A310SMU1   A0A151JV26   A0A087ZPW6   A0A2A3EJH7   A0A195CP85   A0A151HYW1   E9IW27   A0A1B6CQK0   J9JM54   A0A139WGY0   A0A139WGJ9   A0A0M8ZR39   A0A3Q3AHD5   A0A3Q3AHE9   A0A444SRI1   F6Z1A0   A0A218ULG5   A0A2R8MWP2   G5C2X2   A0A151WGS7   A0A091EK02   A0A091CX85   A0A1L8G137   M3XZI5   B0V2Q7   Q3UN02   A0A3B3DKB6   A0A091IA28   V9IH79   L5KT26   A0A402F096   J9NVZ5   G3T046   A0A1D2MM63   K9IXI5   U6DRD8   F6QAZ7   B4F6M8   I3M4Q4   H9F706   A0A151P408   A0A2R8MKZ9   A0A3B3DIV3   A0A2R8ZVQ4   A0A2Y9K6N5   A0A2U3XP59   G1RZV1   A0A3B2WDB3   U3K9X0   A0A3Q7UWD0   A0A2I2Z6V6   Q6UWP7-3   A0A2K5S5T8   A0A1U7RPJ9   A0A1W7RG27   A0A2I3HWC9   A0A1S3EZ85   A0A093GZX9   A0A2K5E023   D3ZFF4   B4DYR5   A0A315UYR5   A0A2U3WL33   A0A2K6DV35   A0A2K5W165   F6QWR0   A0A2I2YN45   Q6UWP7   A0A2Y9HMX2   A0A0D9RFE8   A0A2K5E028   A0A3Q4I762   A0A091H8Y3   A0A2K5K8L4   K7EDA5   A0A1U7Q9A2   G3GW77   A0A091SZY6   A0A3S2MEK2   A0A0Q3LY38   A0A2K6DV17   A0A2K5W176  

KEGG

101745058    K13513 lysocardiolipin and lysophospholipid acyltransferase [EC:2.3.1.- 2.3.1.51] | (RefSeq) lysocar

PATHWAY

00561    Glycerolipid metabolism - Bombyx mori (domestic silkworm)
00564    Glycerophospholipid metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016746   GO:0016021   GO:0022857   GO:0055085   GO:0005783   GO:0036149   GO:0006644   GO:0005829   GO:0016024   GO:0005789   GO:0003841   GO:0007275   GO:0060217   GO:0001885   GO:0008374   GO:0035965   GO:0016020   GO:0006654   GO:0008152   GO:0005215   GO:0005515   GO:0000287   GO:0016620   GO:0016787   GO:0003676   GO:0016491   GO:0015930  

Subcellular location

Endoplasmic reticulum membrane  

Length:

309

Number of predicted TMHs:

2

Exp number of AAs in TMHs:

44.12305

Exp number, first 60 AAs:

0.00544

Total prob of N-in:

0.92547

inside

1  -  247

TMhelix

248  -  270

outside

271  -  273

TMhelix

274  -  296

inside

297  -  309

 

 

Pi

24.735749

Theta

182.809134

Tajima's D

0.132099

CLR

0.669444

CSRT

0.411729413529324

Interpretation

Uncertain