Gene

KWMTBOMO13611  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA011049

Annotation

PREDICTED:_probable_methylthioribulose-1-phosphate_dehydratase_[Amyelois_transitella]

Location

Bomo_Chr23(-):2794652-2798273      View in EpiBrowser

Full name

Probable methylthioribulose-1-phosphate dehydratase       + More
Methylthioribulose-1-phosphate dehydratase      

Alternative Name

APAF1-interacting protein

Location in the cell

Cytoplasmic   Reliability : 3.389

CDS

ATGACGGAAATCGATCCAGAGCATCCTAGGAAATTAATTCCTGAATTATGCAATCAATTTTACCATTTAGGATGGGTGACTGGCACGGGTGGTGGTATATCAATAAAACAAGGAGACAAAATTTACATTGCACCTTCAGGAGTTCAGAAGGAACGCATGAAAGCCAATGATCTGTTTGTCCAAACAATTGATGATGAAGATTTAGAATTACCACCACCGGAAAAAAAGTTGAAGAAAAGTCAATGCACCCCGTTATTTATGTTAGCATATAGAATGCGCAATGCAGGTGCTGTAATACATACACATTCTCCTCACGCAGTCCGATGCACACTGTTGTATGACAAGGTGTTTGAGATCACGCATCAGGAAATGATTAAGGGCATCAAAGACACATCCCTAGGCCGATATCTCCGTTATGATGAGAAATTAGTAGTCCCAATAATAGAAAACACGCCTTTTGAAAAAGATCTAGCCGGTAGTCTGGAAGAAGCTTTAAAGGAATACCCTGGGACTAGTGCAGTGCTCGTCAGAAGACACGGAGTGTACGTATGGGGTGATACGTGGCAACAAGCTAAAACAATGACGGAATGCTATGACTACCTATTCGAGATGGCAGTGGAAATGAAAAAACTAGGACTCGATCCAACTTTTAATCCGGAAACTGCACAAAATGGCCAAAAAACATCCTAG

Protein

MTEIDPEHPRKLIPELCNQFYHLGWVTGTGGGISIKQGDKIYIAPSGVQKERMKANDLFVQTIDDEDLELPPPEKKLKKSQCTPLFMLAYRMRNAGAVIHTHSPHAVRCTLLYDKVFEITHQEMIKGIKDTSLGRYLRYDEKLVVPIIENTPFEKDLAGSLEEALKEYPGTSAVLVRRHGVYVWGDTWQQAKTMTECYDYLFEMAVEMKKLGLDPTFNPETAQNGQKTS

Description

Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P).
Catalyzes the dehydration of methylthioribulose-1-phosphate (MTRu-1-P) into 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P). Functions in the methionine salvage pathway, which plays a key role in cancer, apoptosis, microbial proliferation and inflammation. May inhibit the CASP1-related inflammatory response (pyroptosis), the CASP9-dependent apoptotic pathway and the cytochrome c-dependent and APAF1-mediated cell death.

Catalytic Activity

5-methylsulfanyl-D-ribulose 1-phosphate = 5-methylsulfanyl-2,3-dioxopentyl phosphate + H2O

Cofactor

Zn(2+)

Biophysicochemical Properties

9.3 uM for S-methyl-5-thio-D-ribulose 1-phosphate

Subunit

Homotetramer. Interacts with APAF1. May interact with CASP1.
Homotetramer (PubMed:24367089). Interacts with APAF1 (PubMed:15262985). May interact with CASP1 (PubMed:22837397).

Similarity

Belongs to the aldolase class II family. MtnB subfamily.

Keywords

Alternative initiation   Alternative splicing   Amino-acid biosynthesis   Complete proteome   Cytoplasm   Lyase   Metal-binding   Methionine biosynthesis   Reference proteome   Zinc   3D-structure   Apoptosis   Phosphoprotein   Polymorphism  

Feature

chain  Probable methylthioribulose-1-phosphate dehydratase
splice variant  In isoform E.
sequence variant  In dbSNP:rs2956114.

Uniprot

H9JNE6   A0A2A4JN01   A0A2W1BBH4   S4PT79   A0A212F0G2   A0A0N1PFA1   + More
A0A194PRB6   A0A3S2P8K7   A0A1J1HPY8   A0A182IWB7   T1P951   A0A084VLT5   A0A2K7P6C8   A0A1B0CZC8   A0A2K7P6D4   Q16NX0-2   Q16NX0   U5EJV1   A0A0K8TU72   A0A336MEF2   A0A0Q9WCF6   W5JLZ5   J3JUZ7   B4NEU3   A0A0B6Y7G7   A0A336MIU1   B3MW09   B4M1W5   A0A1I8Q1P6   B4JLL3   A0A2M4AJ53   A0A2M4BY73   A0A3S0ZYW9   A0A023EL85   N6U343   A0A2M4AJD5   A0A2M4BYJ3   A0A2C9K3N2   B3NVZ7   M9PEI9   B4R4E9   B4IG61   Q9VY93   T1E8E1   A0A2M3Z667   A0A1W4VU76   A0A0L7R7W9   B4Q2F5   A0A2M3Z6A8   A0A1Q3FVP3   A0A1B6CGD5   A0A1Y1N8C9   K7JK01   A0A232F2F8   A0A1Q3FYU8   A0A182RHI6   A0A182N174   A0A182Q6P7   A0A182W5J6   A0A034WVF6   A0A0C9QYV9   A0A1L8E1L5   A0A3B0J792   A0A0L0CF23   A0A2P8Z1F4   Q29HV4   A0A182HMK9   Q7PS09   A0A0K8W345   A0A0A1WTR1   D6WY90   A0A1S3DU63   A0A131YCG6   W8BXW6   E2ARR3   A0A182PFX5   A0A067RU59   A0A0M3QYZ2   A0A2T7PKF6   A0A2I3GH41   A0A2R9B3W4   H2Q3E7   Q96GX9   A0A3Q7UN45   G1PZR5   E2C5T1   A0A2K6CXB2   A0A2K6K7P1   A0A2K5ZNX3   A0A0D9QZE7   A0A096NFN2   A0A2K6RRS0   A0A2K5LX17   I2CTP3   F7HS49   A0A2K5FA67   K1QTI9   A0A2K5UVK2   A0A2K5F9Z2   L5MC92  

EC Number

4.2.1.109

Pubmed

19121390    28756777    23622113    22118469    26354079    25315136    + More
24438588    17510324    26369729    17994087    20920257    23761445    22516182    23537049    24945155    15562597    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    12537569    28004739    20075255    28648823    25348373    26108605    29403074    15632085    12364791    25830018    18362917    19820115    26830274    24495485    20798317    24845553    22722832    16136131    10810093    14702039    16554811    15489334    15262985    21269460    21406692    23285211    22837397    23186163    24367089    21993624    25362486    25319552    17431167    22992520   

EMBL

BABH01019230    NWSH01000946    PCG73427.1    KZ150257    PZC71781.1    GAIX01012508    + More
JAA80052.1    AGBW02011095    OWR47222.1    KQ461153    KPJ08653.1    KQ459595    KPI95852.1    RSAL01000002    RVE55016.1    RVE55068.1    CVRI01000013    CRK89594.1    KA644680    AFP59309.1    ATLV01014551    KE524974    KFB38929.1    AJVK01009528    AJVK01009529    CH477804    GANO01002080    JAB57791.1    GDAI01000108    JAI17495.1    UFQS01001072    UFQT01001072    SSX08840.1    SSX28752.1    CH940651    KRF82333.1    ADMH02000670    ETN65387.1    BT127062    KB632255    AEE62024.1    ERL90650.1    CH964239    HACG01005168    CEK52033.1    SSX08841.1    SSX28753.1    CH902625    CH916370    GGFK01007317    MBW40638.1    GGFJ01008896    MBW58037.1    RQTK01000052    RUS89541.1    GAPW01003631    JAC09967.1    APGK01041594    KB740994    ENN75990.1    GGFK01007579    MBW40900.1    GGFJ01009014    MBW58155.1    CH954180    AE014298    AGB95372.1    CM000366    CH480835    AY071553    GAMD01002257    JAA99333.1    GGFM01003265    MBW24016.1    KQ414637    KOC66928.1    CM000162    GGFM01003301    MBW24052.1    GFDL01003509    JAV31536.1    GEDC01024741    JAS12557.1    GEZM01010633    JAV93969.1    NNAY01001140    OXU25006.1    GFDL01002345    JAV32700.1    AXCN02002156    GAKP01000333    JAC58619.1    GBYB01000905    JAG70672.1    GFDF01001464    JAV12620.1    OUUW01000003    SPP78094.1    JRES01000487    KNC30841.1    PYGN01000243    PSN50327.1    CH379064    APCN01008388    AAAB01008846    GDHF01006825    JAI45489.1    GBXI01012055    JAD02237.1    KQ971357    EFA07698.1    GEDV01012329    JAP76228.1    GAMC01000305    JAC06251.1    GL442166    EFN63871.1    KK852463    KDR23379.1    CP012528    ALC48523.1    PZQS01000003    PVD33911.1    ADFV01113403    ADFV01113404    ADFV01113405    ADFV01113406    AJFE02106983    AACZ04016455    GABC01010067    GABF01008012    GABD01010180    GABE01011426    NBAG03000219    JAA01271.1    JAA14133.1    JAA22920.1    JAA33313.1    PNI78661.1    AF132963    AK292648    AC107928    BC008440    BC009077    BC017594    AAPE02035478    AAPE02035479    GL452801    EFN76715.1    AQIB01084554    AQIB01084555    AQIB01084556    AQIB01084557    AQIB01084558    AQIB01084559    AQIB01084560    AQIB01084561    AQIB01084562    AQIB01084563    AHZZ02006654    AHZZ02006655    JV564993    AFJ70328.1    JSUE03012437    JSUE03012438    JU472086    AFH28890.1    JH817826    EKC40092.1    AQIA01020505    KB101864    ELK35971.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000053240    UP000053268    UP000283053    + More
UP000183832    UP000075880    UP000095301    UP000030765    UP000092462    UP000008820    UP000008792    UP000000673    UP000030742    UP000007798    UP000007801    UP000095300    UP000001070    UP000271974    UP000019118    UP000076420    UP000008711    UP000000803    UP000000304    UP000001292    UP000192221    UP000053825    UP000002282    UP000002358    UP000215335    UP000075900    UP000075884    UP000075886    UP000075920    UP000268350    UP000037069    UP000245037    UP000001819    UP000075840    UP000007062    UP000007266    UP000079169    UP000000311    UP000075885    UP000027135    UP000092553    UP000245119    UP000001073    UP000240080    UP000002277    UP000005640    UP000286640    UP000001074    UP000008237    UP000233120    UP000233180    UP000233140    UP000029965    UP000028761    UP000233200    UP000233060    UP000006718    UP000233020    UP000005408    UP000233100   

PRIDE

H9JNE6    M9PEI9    Q9VY93    Q7PS09    Q96GX9   

Pfam

PF00596   Aldolase_II

Interpro

IPR027514   Salvage_MtnB_euk        + More
IPR001303   Aldolase_II/adducin_N       
IPR017714   MethylthioRu-1-P_deHdtase_MtnB       
IPR036409   Aldolase_II/adducin_N_sf       

SUPFAM

SSF53639   SSF53639       

Gene 3D

3.40.225.10    

ProteinModelPortal

H9JNE6   A0A2A4JN01   A0A2W1BBH4   S4PT79   A0A212F0G2   A0A0N1PFA1   + More
A0A194PRB6   A0A3S2P8K7   A0A1J1HPY8   A0A182IWB7   T1P951   A0A084VLT5   A0A2K7P6C8   A0A1B0CZC8   A0A2K7P6D4   Q16NX0-2   Q16NX0   U5EJV1   A0A0K8TU72   A0A336MEF2   A0A0Q9WCF6   W5JLZ5   J3JUZ7   B4NEU3   A0A0B6Y7G7   A0A336MIU1   B3MW09   B4M1W5   A0A1I8Q1P6   B4JLL3   A0A2M4AJ53   A0A2M4BY73   A0A3S0ZYW9   A0A023EL85   N6U343   A0A2M4AJD5   A0A2M4BYJ3   A0A2C9K3N2   B3NVZ7   M9PEI9   B4R4E9   B4IG61   Q9VY93   T1E8E1   A0A2M3Z667   A0A1W4VU76   A0A0L7R7W9   B4Q2F5   A0A2M3Z6A8   A0A1Q3FVP3   A0A1B6CGD5   A0A1Y1N8C9   K7JK01   A0A232F2F8   A0A1Q3FYU8   A0A182RHI6   A0A182N174   A0A182Q6P7   A0A182W5J6   A0A034WVF6   A0A0C9QYV9   A0A1L8E1L5   A0A3B0J792   A0A0L0CF23   A0A2P8Z1F4   Q29HV4   A0A182HMK9   Q7PS09   A0A0K8W345   A0A0A1WTR1   D6WY90   A0A1S3DU63   A0A131YCG6   W8BXW6   E2ARR3   A0A182PFX5   A0A067RU59   A0A0M3QYZ2   A0A2T7PKF6   A0A2I3GH41   A0A2R9B3W4   H2Q3E7   Q96GX9   A0A3Q7UN45   G1PZR5   E2C5T1   A0A2K6CXB2   A0A2K6K7P1   A0A2K5ZNX3   A0A0D9QZE7   A0A096NFN2   A0A2K6RRS0   A0A2K5LX17   I2CTP3   F7HS49   A0A2K5FA67   K1QTI9   A0A2K5UVK2   A0A2K5F9Z2   L5MC92  

PDB

4M6R     E-value=3.03007e-80,     Score=757

KEGG

101745355    K08964 methylthioribulose-1-phosphate dehydratase [EC:4.2.1.109] | (RefSeq) probable methylthioribul

PATHWAY

00270    Cysteine and methionine metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0005737   GO:0019509   GO:0046570   GO:0019284   GO:0008270   GO:0046872   GO:0043066   GO:0070269   GO:0042802   GO:0070372   GO:0051289   GO:0006915   GO:0005829   GO:0006508   GO:0008237   GO:0008152   GO:0016787   GO:0003676   GO:0016491   GO:0015930  

PANTHER

PTHR10640  

Subcellular location

Cytoplasm  

Length:

229

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0409700000000001

Exp number, first 60 AAs:

0.04037

Total prob of N-in:

0.05329

outside

1  -  229

 

 

Pi

380.58876

Theta

201.464226

Tajima's D

2.758413

CLR

1.144112

CSRT

0.966951652417379

Interpretation

Uncertain