Gene

KWMTBOMO13596  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA011003

Annotation

PREDICTED:_UDP-N-acetylglucosamine_transferase_subunit_ALG13_homolog_[Plutella_xylostella]

Location

Bomo_Chr23(+):2488654-2489216      View in EpiBrowser

Full name

Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13      

Alternative Name

Asparagine-linked glycosylation 13 homolog
Glycosyltransferase 28 domain-containing protein 1
UDP-N-acetylglucosamine transferase subunit ALG13 homolog

Location in the cell

Cytoplasmic   Reliability : 3.179

CDS

ATGCTTTCACCAGCCGTAATTCATGCACTAAAGGAGATCGGCTGCAAAGAGGTTGTAATACAAATTGGCAATAGTGACGTAGATCCAGGAAATTTTACTAAAAACGAGATTAAGTTCACTTTGTACAGATTTAAGGACTCTATATTAGAAGATATAAAAAAAGCCGATTTAGTTATAAGTCACGCCGGAGCAGGAAGCTGTCTTGAAGTGTTAGAAGAAAAGAAACCTCTTTTAGTGGTTGTTAACGAGGATTTGATGGACAACCACCAACTAGAACTCGCAGAACAGCTCCAAATAGATGGACATTTATACTATTGTACTTGCGATACTCTGGTCAACACACTAAATGTTGTTGACTTTGAATTATTACGTCCCTTTCCCAAAGCAAATCCTATTTTGTTTGTCAGATATTTGGATGATATTTTCAGAGTATATAAAAATATAGACTGA

Protein

MLSPAVIHALKEIGCKEVVIQIGNSDVDPGNFTKNEIKFTLYRFKDSILEDIKKADLVISHAGAGSCLEVLEEKKPLLVVVNEDLMDNHQLELAEQLQIDGHLYYCTCDTLVNTLNVVDFELLRPFPKANPILFVRYLDDIFRVYKNID

Catalytic Activity

N-acetyl-alpha-D-glucosaminyl-diphosphodolichol + UDP-N-acetyl-alpha-D-glucosamine = H(+) + N,N'-diacetylchitobiosyl diphosphodolichol + UDP
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit

Isoform 2 may interact with ALG14.

Similarity

Belongs to the glycosyltransferase 28 family.

Keywords

Alternative splicing   Complete proteome   Congenital disorder of glycosylation   Disease mutation   Endoplasmic reticulum   Epilepsy   Glycosyltransferase   Hydrolase   Multifunctional enzyme   Polymorphism   Protease   Reference proteome   Thiol protease   Transferase   Ubl conjugation pathway  

Feature

chain  Putative bifunctional UDP-N-acetylglucosamine transferase and deubiquitinase ALG13
splice variant  In isoform 4.
sequence variant  In EIEE36; disease features include abnormal isoelectric focusing of serum transferrin consistent with a glycosylation defect; enzyme activity at about 17% of wild-type; dbSNP:rs867599353.

Uniprot

H9JNA0   A0A2W1BX24   A0A2H1WXA9   A0A0L7LHT1   A0A0N1I546   A0A3S2TTT7   + More
S4PL85   V9LJ87   F7BAW9   A0A151PDS9   A0A3Q0RTP5   K1RHC5   A0A2D4KJU8   A0A2D4PJ01   F7E7G8   A0A2P4TIL5   A0A1D5PWJ2   A0A3Q3ESM9   A0A226NJC2   A0A226P6D7   A0A3B4G942   A0A3Q2VT39   A0A3Q4GBN7   I3JD92   A0A3P9JIF7   A0A3B3HEY0   Q4RP99   A0A3Q3EP66   A0A3P9LDW7   A0A0K8RYE1   A0A3S2MUV2   A0A3P9BA76   A0A3P8QC11   A0A3B3CER7   A0A3B5KPX6   A0A2I4AZX6   A0A1L1RT72   A0A3P8X979   A0A2I0UAA5   A0A3Q2TDJ1   A0A146P5A6   A0A1A8JYN3   A0A1A8EJN9   V8NLS8   A0A3B4AZ89   A0A1A8SGB6   A0A1V4KYP4   A0A1A8N2Q8   A0A1A8BTL9   A0A1A7ZBL4   G3PXZ1   A0A3Q3VNA0   A0A3Q2DAF6   A0A2U9C8I5   A0A2I3MNS4   A0A3Q2ECG1   A0A287B9F4   A0A1A7X791   A0A3P8V6M2   A0A3Q2ZNN7   A0A341BQJ4   A0A3B3WYA9   A0A3Q1IID4   U3FXV1   A0A1W4YLS7   A0A2K5DDH0   W5NCI7   A0A2K6SFU3   A0A2J8VAF9   A0A2Y9DRL6   S7NRJ4   A0A2K5PW92   F6YIZ0   A0A2K5JKH5   A0A2R9CST1   H2QZ05   A0A2K6CHW9   A0A2K5VHX9   F7CXN6   Q9NP73-2   A0A2K5ZYT9   A0A2K5LYR0   A0A2I3G9S4   E2RPL3   A0A087XS90   A0A2I2U8L0   M1ECH4   M4AF83   A0A3B5MIM4   A0A315VHN8   K9IYD3   F6PT67   A0A1U7R7C4   A0A061HXS5   A0A3B3U2T6   A0A3Q0CGD1   A0A2K6PVW6   A0A3P9NTD5   A0A0A9ZGQ7   A0A3S3Q999  

Pubmed

19121390    28756777    26227816    26354079    23622113    24402279    + More
18464734    22293439    22992520    17495919    15592404    24621616    25186727    17554307    15496914    25727380    29451363    21551351    25069045    24297900    25463417    30723633    24487278    25243066    22722832    16136131    17431167    22002653    25319552    14702039    15772651    15489334    16100110    23827681    21269460    22492991    23934111    26138355    23033978    27476654    16341006    17975172    23236062    23542700    29703783    19892987    23929341    29704459    28071753    25362486    25401762   

EMBL

BABH01033318    KZ149909    PZC78195.1    ODYU01011769    SOQ57700.1    JTDY01001053    + More
KOB75007.1    KQ461153    KPJ08641.1    RSAL01000002    RVE55006.1    RVE55059.1    GAIX01004175    JAA88385.1    JW880764    AFP13281.1    AAPN01289039    AAPN01289040    AAPN01289041    AKHW03000474    KYO47267.1    JH819176    EKC33531.1    IACL01071176    LAB08968.1    IACN01075325    IACN01075326    LAB57458.1    PPHD01000017    POI36184.1    AADN05000020    MCFN01000034    OXB67624.1    AWGT02000146    OXB75411.1    AERX01012441    AERX01012442    CAAE01015008    CAG09783.1    GBKC01000176    JAG45894.1    CM012446    RVE67368.1    KZ505944    PKU42980.1    GCES01147209    JAQ39113.1    HAED01014544    HAEE01005042    SBR25062.1    HAEB01000570    HAEC01015341    SBQ47022.1    AZIM01002841    ETE63249.1    HAEH01014405    HAEI01014912    SBS17381.1    LSYS01001150    OPJ89401.1    HAEF01003022    HAEG01000169    SBR63373.1    HADZ01006608    HAEA01013032    SBP70549.1    HADY01001221    HAEJ01018750    SBP39706.1    CP026255    AWP11939.1    AHZZ02038378    AEMK02000121    DQIR01257397    HDC12875.1    HADW01012526    HADX01015210    SBP13926.1    GAMP01001430    JAB51325.1    AHAT01016800    NDHI03003425    PNJ54500.1    KE164818    EPQ20339.1    GAMT01000278    GAMS01002024    GAMR01004478    GAMQ01003576    JAB11583.1    JAB21112.1    JAB29454.1    JAB38275.1    AJFE02076159    AJFE02076160    AC193880    GABC01004694    GABF01005266    GABD01009523    GABD01009522    GABE01004809    NBAG03000329    JAA06644.1    JAA16879.1    JAA23577.1    JAA39930.1    PNI40053.1    AQIA01086487    JSUE03046497    JSUE03046498    JU336437    JU470726    CM001273    AFE80190.1    AFH27530.1    EHH31003.1    AF220051    AK026671    AK300394    AK302729    AK302890    AK304712    AK316306    AK316522    AK312229    AL049563    AL096764    CH471120    BC005336    BC117377    BC117379    AK223154    ADFV01008300    ADFV01008301    ADFV01008302    AAEX03026776    AYCK01006064    AANG04001034    JP005447    AER94044.1    NHOQ01002284    PWA18656.1    GABZ01008291    JAA45234.1    KE685696    RAZU01000333    ERE65171.1    RLQ59675.1    GBHO01002395    GBHO01002394    JAG41209.1    JAG41210.1    NCKU01001935    NCKU01000276    RWS10865.1    RWS16183.1   

Proteomes

UP000005204    UP000037510    UP000053240    UP000283053    UP000002279    UP000050525    + More
UP000261340    UP000005408    UP000002280    UP000000539    UP000261660    UP000198323    UP000198419    UP000261460    UP000264840    UP000261580    UP000005207    UP000265200    UP000001038    UP000007303    UP000265180    UP000265160    UP000265100    UP000261560    UP000005226    UP000192220    UP000265140    UP000265000    UP000261520    UP000190648    UP000007635    UP000261620    UP000265020    UP000246464    UP000028761    UP000008227    UP000265120    UP000264800    UP000252040    UP000261480    UP000265040    UP000192224    UP000233020    UP000018468    UP000233220    UP000248480    UP000233040    UP000008225    UP000233080    UP000240080    UP000002277    UP000233120    UP000233100    UP000006718    UP000005640    UP000233140    UP000233060    UP000001073    UP000002254    UP000028760    UP000011712    UP000002852    UP000261380    UP000002281    UP000189706    UP000030759    UP000273346    UP000261500    UP000233200    UP000242638    UP000285301   

PRIDE

H9JNA0    Q9NP73    F6PT67   

Pfam

PF04101   Glyco_tran_28_C        + More
PF02338   OTU

Interpro

IPR007235   Glyco_trans_28_C        + More
IPR039042   Alg13-like       
IPR004259   PP_M1       
IPR003323   OTU_dom       
IPR038765   Papain-like_cys_pep_sf       
IPR002999   Tudor       

SUPFAM

SSF54001   SSF54001       

ProteinModelPortal

H9JNA0   A0A2W1BX24   A0A2H1WXA9   A0A0L7LHT1   A0A0N1I546   A0A3S2TTT7   + More
S4PL85   V9LJ87   F7BAW9   A0A151PDS9   A0A3Q0RTP5   K1RHC5   A0A2D4KJU8   A0A2D4PJ01   F7E7G8   A0A2P4TIL5   A0A1D5PWJ2   A0A3Q3ESM9   A0A226NJC2   A0A226P6D7   A0A3B4G942   A0A3Q2VT39   A0A3Q4GBN7   I3JD92   A0A3P9JIF7   A0A3B3HEY0   Q4RP99   A0A3Q3EP66   A0A3P9LDW7   A0A0K8RYE1   A0A3S2MUV2   A0A3P9BA76   A0A3P8QC11   A0A3B3CER7   A0A3B5KPX6   A0A2I4AZX6   A0A1L1RT72   A0A3P8X979   A0A2I0UAA5   A0A3Q2TDJ1   A0A146P5A6   A0A1A8JYN3   A0A1A8EJN9   V8NLS8   A0A3B4AZ89   A0A1A8SGB6   A0A1V4KYP4   A0A1A8N2Q8   A0A1A8BTL9   A0A1A7ZBL4   G3PXZ1   A0A3Q3VNA0   A0A3Q2DAF6   A0A2U9C8I5   A0A2I3MNS4   A0A3Q2ECG1   A0A287B9F4   A0A1A7X791   A0A3P8V6M2   A0A3Q2ZNN7   A0A341BQJ4   A0A3B3WYA9   A0A3Q1IID4   U3FXV1   A0A1W4YLS7   A0A2K5DDH0   W5NCI7   A0A2K6SFU3   A0A2J8VAF9   A0A2Y9DRL6   S7NRJ4   A0A2K5PW92   F6YIZ0   A0A2K5JKH5   A0A2R9CST1   H2QZ05   A0A2K6CHW9   A0A2K5VHX9   F7CXN6   Q9NP73-2   A0A2K5ZYT9   A0A2K5LYR0   A0A2I3G9S4   E2RPL3   A0A087XS90   A0A2I2U8L0   M1ECH4   M4AF83   A0A3B5MIM4   A0A315VHN8   K9IYD3   F6PT67   A0A1U7R7C4   A0A061HXS5   A0A3B3U2T6   A0A3Q0CGD1   A0A2K6PVW6   A0A3P9NTD5   A0A0A9ZGQ7   A0A3S3Q999  

PDB

2JZC     E-value=4.77609e-06,     Score=114

KEGG

101742931    K07432 beta-1,4-N-acetylglucosaminyltransferase [EC:2.4.1.141] | (RefSeq) UDP-N-acetylglucosamine tr

PATHWAY

00510    N-Glycan biosynthesis - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0006488   GO:0016758   GO:0003968   GO:0019083   GO:0036459   GO:0004577   GO:0005789   GO:0003723   GO:0016740   GO:0016020   GO:0006520   GO:0006508   GO:0008237   GO:0008152   GO:0016787   GO:0003676   GO:0016491   GO:0015930  

PANTHER

PTHR12867  

Subcellular location

Endoplasmic reticulum  

Length:

149

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.000900000000000001

Exp number, first 60 AAs:

0

Total prob of N-in:

0.04069

outside

1  -  149

 

 

Pi

165.615708

Theta

187.059252

Tajima's D

-0.360734

CLR

0.434092

CSRT

0.273036348182591

Interpretation

Uncertain