Gene

KWMTBOMO13399

Pre Gene Modal

BGIBMGA010907

Annotation

PREDICTED:_FAD_synthase-like_isoform_X1_[Papilio_machaon]

Location

Bomo_Chr22(+):16135063-16150775      View in EpiBrowser

Full name

tRNA (guanine(37)-N1)-methyltransferase       + More
FAD synthase      

Alternative Name

M1G-methyltransferase
tRNA [GM37] methyltransferase
tRNA methyltransferase 5 homolog
FAD pyrophosphorylase
FMN adenylyltransferase
Flavin adenine dinucleotide synthase

Location in the cell

Cytoplasmic   Reliability : 1.414 Extracellular   Reliability : 1.165 Nuclear   Reliability : 1.314

CDS

ATGCAACCGACGTGCGGAGGCATGGACGAGCTACCTGACGTTACTGATGTCCTGAAGGAAGCCGAGCAGGTAATTAGGCAATGCTTCCAAACGTACGCATTGGACGAGGTATTCTTATGTTTCAACGGCGGAAAAGACTGCACAGTATTATTAGACATCACAATAAACGTACTTAAAGATATTTACAAGAGCTGCGACATAGGGAAGAACCTTAAAGTCGTCTACATAAGGACAAAGGGACCGTTTGTCGAAATCGAGAAATTCGTTCAAGAGATTAAAATGTATTATGGTCTGACGTTAAAAGTAACCGAAGGCGAAATGAAGGAGACGTTACAGAGGCTATTAGAGAAGGACGGAATATTGAAGGCCGGCTTGATGGGAACGAGACGGAGCGATCCTTACAGCGAGAATTTGCAATTTGTTCAGAAAACGGATGCGAATTGGCCTCAGATAATGAGAATATCTCCGCTATTGAACTGGTTTTATCACCACATATGGAGCTACATATTGCAACGACAAGTGCCGTACTGCAGTCTCTACGATAAAGGGTACACGTCGATAGGAAGCACAACGAACACGTGGCCGAATCCAGCCTTAGCCCACAAGGACTGCTTCGGTTGCGTCACCTACCACCCCGCCTGGAGATTATCCGACGCGTCCTTAGAGAGAGCCGGTCGAGGAAGTTCAGTGAAGGCACCCGTCAATGGAAACGGTACATATCACAATCATAGTTTGACGTCAACCATCAATCCTTGTATACGCAACGGTGATGTTCTTTGA

Protein

MQPTCGGMDELPDVTDVLKEAEQVIRQCFQTYALDEVFLCFNGGKDCTVLLDITINVLKDIYKSCDIGKNLKVVYIRTKGPFVEIEKFVQEIKMYYGLTLKVTEGEMKETLQRLLEKDGILKAGLMGTRRSDPYSENLQFVQKTDANWPQIMRISPLLNWFYHHIWSYILQRQVPYCSLYDKGYTSIGSTTNTWPNPALAHKDCFGCVTYHPAWRLSDASLERAGRGSSVKAPVNGNGTYHNHSLTSTINPCIRNGDVL

Description

Specifically methylates the N1 position of guanosine-37 in various cytoplasmic and mitochondrial tRNAs. Methylation is not dependent on the nature of the nucleoside 5' of the target nucleoside. This is the first step in the biosynthesis of wybutosine (yW), a modified base adjacent to the anticodon of tRNAs and required for accurate decoding.
Catalyzes the adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme.

Catalytic Activity

guanosine(37) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-methylguanosine(37) in tRNA + S-adenosyl-L-homocysteine
ATP + FMN + H(+) = diphosphate + FAD

Cofactor

Mg(2+)

Subunit

Monomer.

Similarity

Belongs to the TRM5 / TYW2 family.
Belongs to the class I-like SAM-binding methyltransferase superfamily. TRM5/TYW2 family.
Belongs to the AAA ATPase family.
In the C-terminal section; belongs to the PAPS reductase family. FAD1 subfamily.
In the N-terminal section; belongs to the MoaB/Mog family.

Uniprot

A0A2H1V1S0   A0A194RJ13   S4PD19   A0A212ES28   A0A182MSM2   Q16R18   + More
A0A182RT01   A0A182JPS7   A0A023EJW0   A0A067RJ45   A0A182UUE1   A0A182HS54   A0A182PTK6   A0A182VZR8   A0A182XAA9   A0A182PUF7   A0A182TEY1   A0A182QMH8   A0A2M4C0J5   A0A2M4ASX7   A0A0T6B8S1   Q7PR81   A0A182LE17   A0A1S3JLB7   W5JFH8   A0A1S3JK05   A0A182FQW9   A0A182Y3X9   A0A1Q3FX95   A0A0P4W0W7   A0A182NQI2   R7TAB2   A0A336KMK0   K1R7T5   A0A084VHR3   A0A1Y1K9C7   S4RHL1   A0A224XBA9   A0A336LH97   F5HKN2   A0A0P4W1D7   A0A1J1IF22   T1GTI8   A0A3R7PN01   B4IEL7   B4Q4T8   V4BL84   A0A0P4W8U6   A0A087UBU6   U4U573   Q8MZ99   Q9VJY1   T1HF00   A0A1W4VKX7   B3MS20   A0A2C9LXT6   E0VLV1   W8CBV0   A0A091I5W9   A0A0P9AA87   A0A3Q3IK62   A0A2C9LXT3   A0A1W4V7U0   B4IGK2   B4P369   A0A210QUG9   A0A0M4EXK7   A0A3M6U4U2   B4M8I2   A0A2Z6SND3   A0A2G8JXV7   A0A2B4RPN7   A0A3Q2QZ39   A0A0P6AJA4   A0A0M8ZRA3   A0A034WDF5   A0A146WT70   A0A2H6N099   A0A146WSC9   U3IP73   A0A372Q6K6   A0A2G8K5S1   A0A0R1E9W5   A0A2P4P0M3   A0A015JDC8   A0A397I2T9   A0A0A1X3K3   A0A146WSL6   A0A3Q0RSU9   A0A0B6Z746   A0A1B6G5V1   B3NAW5   A0A0N8B0I2   V5G434   B4Q1Y5   N6TD15   A0A0K8UPH6   A0A0K2U4F6  

EC Number

2.1.1.228
2.7.7.2

Pubmed

26354079    23622113    22118469    17510324    24945155    24845553    + More
12364791    14747013    17210077    20966253    20920257    23761445    25244985    27129103    23254933    22992520    24438588    28004739    17994087    22936249    23537049    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    18057021    15562597    20566863    24495485    17550304    28812685    30382153    29023486    25348373    24277808    29355972    25830018    24407650   

EMBL

ODYU01000073    SOQ34214.1    KQ460323    KPJ15931.1    GAIX01003831    JAA88729.1    + More
AGBW02012888    OWR44285.1    AXCM01000621    CH477726    EAT36856.1    GAPW01004226    JAC09372.1    KK852654    KDR19338.1    APCN01000243    AXCN02001534    GGFJ01009367    MBW58508.1    GGFK01010397    MBW43718.1    LJIG01009117    KRT83722.1    AAAB01008859    EAA07691.5    ADMH02001643    ETN61635.1    GFDL01002838    JAV32207.1    GDKW01001249    JAI55346.1    AMQN01003072    KB310836    ELT90649.1    UFQS01000644    UFQT01000644    SSX05689.1    SSX26048.1    JH816527    EKC37260.1    ATLV01013213    KE524845    KFB37507.1    GEZM01088633    JAV58059.1    GFTR01006720    JAW09706.1    UFQS01004045    UFQT01004045    SSX16127.1    SSX35454.1    EGK96784.1    GDRN01080441    JAI62219.1    CVRI01000047    CRK97598.1    CAQQ02030687    QCYY01002259    ROT71711.1    CH480831    EDW46044.1    CM000361    CM002910    EDX04926.1    KMY90101.1    KB202619    ESO89349.1    GDRN01080440    JAI62220.1    KK119125    KFM74835.1    KB631957    ERL87473.1    AY113289    AAM29294.1    AE014134    BT150141    AAF53304.1    AGO63484.1    ACPB03013538    CH902622    EDV34575.1    KPU75203.1    DS235281    EEB14357.1    GAMC01004492    GAMC01004491    JAC02065.1    KL218155    KFP02790.1    KPU75202.1    CH480836    EDW48952.1    CM000157    EDW88311.1    NEDP02001812    OWF52384.1    CP012528    ALC48505.1    RCHS01002246    RMX48606.1    CH940654    EDW57508.1    BEXD01004265    GBC09017.1    MRZV01001112    PIK40525.1    LSMT01000423    PFX18225.1    GDIP01041947    JAM61768.1    KQ435955    KOX68033.1    GAKP01007144    GAKP01007143    JAC51808.1    GCES01054158    JAR32165.1    IACI01027327    LAA21515.1    GCES01054159    JAR32164.1    QKKE01000956    RGB23641.1    MRZV01000861    PIK43299.1    CM000162    KRK06186.1    KRK06187.1    KRK06188.1    AUPC02000486    POG58932.1    JEMT01017717    EXX67487.1    PQFF01000259    RHZ69482.1    GBXI01017523    GBXI01008383    JAC96768.1    JAD05909.1    GCES01054157    JAR32166.1    HACG01016896    CEK63761.1    GECZ01011959    JAS57810.1    CH954177    EDV58679.1    GDIQ01224803    JAK26922.1    BAUL01000283    GAD99193.1    EDX01506.1    APGK01043140    KB741011    ENN75578.1    GDHF01028110    GDHF01026607    GDHF01023727    GDHF01023142    GDHF01009567    JAI24204.1    JAI25707.1    JAI28587.1    JAI29172.1    JAI42747.1    HACA01015450    HACA01015451    CDW32812.1   

Proteomes

UP000053240    UP000007151    UP000075883    UP000008820    UP000075900    UP000075881    + More
UP000027135    UP000075903    UP000075840    UP000075885    UP000075920    UP000076407    UP000075902    UP000075886    UP000007062    UP000075882    UP000085678    UP000000673    UP000069272    UP000076408    UP000075884    UP000014760    UP000005408    UP000030765    UP000245300    UP000183832    UP000015102    UP000283509    UP000001292    UP000000304    UP000030746    UP000054359    UP000030742    UP000000803    UP000015103    UP000192221    UP000007801    UP000076420    UP000009046    UP000054308    UP000261600    UP000002282    UP000242188    UP000092553    UP000275408    UP000008792    UP000247702    UP000230750    UP000225706    UP000265000    UP000053105    UP000263633    UP000018888    UP000022910    UP000266861    UP000261340    UP000008711    UP000018001    UP000019118   

PRIDE

Q8MZ99    Q9VJY1   

Pfam

PF01507   PAPS_reduct        + More
PF02475   Met_10
PF00994   MoCF_biosynth
PF00004   AAA
PF17862   AAA_lid_3

Interpro

IPR030382   MeTrfase_TRM5/TYW2        + More
IPR025792   tRNA_Gua_MeTrfase_euk       
IPR029063   SAM-dependent_MTases       
IPR002500   PAPS_reduct       
IPR014729   Rossmann-like_a/b/a_fold       
IPR001453   MoaB/Mog_dom       
IPR036425   MoaB/Mog-like_dom_sf       
IPR003960   ATPase_AAA_CS       
IPR027417   P-loop_NTPase       
IPR003959   ATPase_AAA_core       
IPR005937   26S_Psome_P45-like       
IPR003593   AAA+_ATPase       
IPR041569   AAA_lid_3       
IPR035245   PSMC2       
IPR012183   FAD_synth_MoaB/Mog-bd       

SUPFAM

SSF53335   SSF53335        + More
SSF53218   SSF53218       
SSF52540   SSF52540       

Gene 3D

3.40.50.620     3.40.980.10    

CDD

cd01713  PAPS_reductase
cd00885  cinA

ProteinModelPortal

A0A2H1V1S0   A0A194RJ13   S4PD19   A0A212ES28   A0A182MSM2   Q16R18   + More
A0A182RT01   A0A182JPS7   A0A023EJW0   A0A067RJ45   A0A182UUE1   A0A182HS54   A0A182PTK6   A0A182VZR8   A0A182XAA9   A0A182PUF7   A0A182TEY1   A0A182QMH8   A0A2M4C0J5   A0A2M4ASX7   A0A0T6B8S1   Q7PR81   A0A182LE17   A0A1S3JLB7   W5JFH8   A0A1S3JK05   A0A182FQW9   A0A182Y3X9   A0A1Q3FX95   A0A0P4W0W7   A0A182NQI2   R7TAB2   A0A336KMK0   K1R7T5   A0A084VHR3   A0A1Y1K9C7   S4RHL1   A0A224XBA9   A0A336LH97   F5HKN2   A0A0P4W1D7   A0A1J1IF22   T1GTI8   A0A3R7PN01   B4IEL7   B4Q4T8   V4BL84   A0A0P4W8U6   A0A087UBU6   U4U573   Q8MZ99   Q9VJY1   T1HF00   A0A1W4VKX7   B3MS20   A0A2C9LXT6   E0VLV1   W8CBV0   A0A091I5W9   A0A0P9AA87   A0A3Q3IK62   A0A2C9LXT3   A0A1W4V7U0   B4IGK2   B4P369   A0A210QUG9   A0A0M4EXK7   A0A3M6U4U2   B4M8I2   A0A2Z6SND3   A0A2G8JXV7   A0A2B4RPN7   A0A3Q2QZ39   A0A0P6AJA4   A0A0M8ZRA3   A0A034WDF5   A0A146WT70   A0A2H6N099   A0A146WSC9   U3IP73   A0A372Q6K6   A0A2G8K5S1   A0A0R1E9W5   A0A2P4P0M3   A0A015JDC8   A0A397I2T9   A0A0A1X3K3   A0A146WSL6   A0A3Q0RSU9   A0A0B6Z746   A0A1B6G5V1   B3NAW5   A0A0N8B0I2   V5G434   B4Q1Y5   N6TD15   A0A0K8UPH6   A0A0K2U4F6  

PDB

3G6K     E-value=4.10112e-25,     Score=282

KEGG

101736382    K00953 FAD synthetase [EC:2.7.7.2] | (RefSeq) FAD synthase

PATHWAY

00740    Riboflavin metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0052906   GO:0005759   GO:0005634   GO:0003824   GO:0016740   GO:0006747   GO:0003919   GO:0005524   GO:0030163   GO:0036402   GO:0005737   GO:0016779   GO:0004604   GO:0008152   GO:0007018   GO:0008017   GO:0005525   GO:0031386   GO:0048869   GO:0003676   GO:0016491   GO:0015930  

PANTHER

PTHR23073:SF13  

Subcellular location

Cytoplasm   Predominantly in the mitochondria and in the nucleus.   With evidence from 1 publications.
Mitochondrion matrix   Predominantly in the mitochondria and in the nucleus.   With evidence from 1 publications.
Nucleus   Predominantly in the mitochondria and in the nucleus.   With evidence from 1 publications.

Length:

259

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.00987

Exp number, first 60 AAs:

0.00088

Total prob of N-in:

0.03188

outside

1  -  259

 

 

Pi

37.495695

Theta

25.781048

Tajima's D

-1.106918

CLR

1.244701

CSRT

0.11624418779061

Interpretation

Uncertain