Gene

KWMTBOMO13202

Annotation

PREDICTED:_serine/threonine-protein_kinase_Nek8-like_[Bombyx_mori]

Location

Bomo_Chr22(-):10079326-10082656      View in EpiBrowser

Full name

Serine/threonine-protein kinase Nek8      

Alternative Name

Never in mitosis A-related kinase 8
Nima-related protein kinase 12a

Location in the cell

PlasmaMembrane   Reliability : 2.351

CDS

ATGGATCTACAGAATCGTTTTACTAATCAAAAATTGTCAATATTGAAGACAATAGGCAAAGGAACTTACGGAAATGTGTACTTGTGCGAGAATCATGAAAACCATTTATTAACTATTGTAAAAGATATTCAACTGAACGTCAAAATACAACAACATGAACAGGACATCGCAAACGAAGTTAGAATTTTATCTACTATGAAACATCCCAACATCATTAGTTTCTACCAGTGCTTCTACGCCGAAGATTACGTAATGATATCAATGGAATACGCAACTTGTGGAAATTTAGCCGAGTTCATGTACCTTCGGTGCCCGCAAGTCATCAAACAAGAGGAAATTCTGTTCTACTTTTGCCAAATACTCTTAGGCGTAAGTTACATTCACAATTTGGACATCATTCACCGAGATTTGAAAGCAGAAAATATCTTACTGACTGGAAAATATGGTATAATAGTTAAAATAGGCGACTTTGGAATTTCAAAAATGTTAGCAAGTGCCAAGAAGACTTCAACAGTCATCGGAACACCTTACTACTTGGCGCCGGAACTATGCGAAGGTCAACCTTATGACACGAAAAGCGACATTTGGGCGCTCGGCTGTTTGCTTTACGAAATGTGTACACATAAACGTGCTTTTGAATCGGAGACTCTCGTCGGTCTAGTCAAAGCAATCACAAGTGGTAGCGTCCACCCTATCGACTTGTTTTTATACGACAGAGGGATGCAAGACCTTATTGATTCGATGCTGTCAATATTGCCAAACAAAAGGCCGACCATCAAAGAAGTTATGGGAAAAGAAATCATTTTACCCGTTATTTACACGGTTTATTTGGACGCGGGTGACGACGATTTATTGATTTTGAAAGCTAAAGAATTTATGTGA

Protein

MDLQNRFTNQKLSILKTIGKGTYGNVYLCENHENHLLTIVKDIQLNVKIQQHEQDIANEVRILSTMKHPNIISFYQCFYAEDYVMISMEYATCGNLAEFMYLRCPQVIKQEEILFYFCQILLGVSYIHNLDIIHRDLKAENILLTGKYGIIVKIGDFGISKMLASAKKTSTVIGTPYYLAPELCEGQPYDTKSDIWALGCLLYEMCTHKRAFESETLVGLVKAITSGSVHPIDLFLYDRGMQDLIDSMLSILPNKRPTIKEVMGKEIILPVIYTVYLDAGDDDLLILKAKEFM

Description

Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway (By similarity).
Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia (By similarity). Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.
Required for renal tubular integrity. May regulate local cytoskeletal structure in kidney tubule epithelial cells. May regulate ciliary biogenesis through targeting of proteins to the cilia. Plays a role in organogenesis and is involved in the regulation of the Hippo signaling pathway.

Catalytic Activity

ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-[protein]
ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-threonyl-[protein]

Cofactor

Mg(2+)

Subunit

Interacts with PKD2; may regulate PKD2 targeting to the cilium. Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3 (PubMed:23793029). ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target it to the proximal ciliary axoneme. Interacts with ANKS3 (By similarity).
Interacts with PKD2; may regulate PKD2 targeting to the cilium (By similarity). Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3. ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target the complex to the proximal ciliary axoneme. Interacts with ANKS3 (By similarity).
Interacts with PKD2; may regulate PKD2 targeting to the cilium. Component of a complex containing at least ANKS6, INVS, NEK8 and NPHP3. ANKS6 may organize complex assembly by linking INVS and NPHP3 to NEK8 and INVS may target the complex to the proximal ciliary axoneme (By similarity). Interacts with ANKS3 (PubMed:25671767).

Similarity

Belongs to the protein kinase superfamily.
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.

Keywords

ATP-binding   Cell projection   Cilium   Complete proteome   Cytoplasm   Cytoskeleton   Disease mutation   Kinase   Magnesium   Metal-binding   Nucleotide-binding   Phosphoprotein   Reference proteome   Repeat   Transferase   Ciliopathy   Nephronophthisis  

Feature

chain  Serine/threonine-protein kinase Nek8
sequence variant  In NPHP9; a full-length mouse NEK8 construct containing the mutation shows a defect in ciliary localization with no apparent effect on ciliation, mitosis or centriole number; dbSNP:rs199962228.

Uniprot

A0A212F1I3   A0A2A4J3N5   A0A194PY50   A0A3S2LCX9   A0A194R0U8   A0A0L7LCN4   + More
A0A0L7K4C3   A0A1Y1MT33   J9NYR9   H9H1W0   A0A146YJB5   A0A2J8LIA4   K7EL04   A0A2J8TMM9   C3ZJ42   A0A3M0KIA4   B4DW95   H0Z713   A0A1S3H2T7   A0A1S3F541   A0A2R2MJK9   A0A1V4K2E2   A0A218V1J8   A0A2I0LKT7   A0A384CH41   E1BRZ2   A0A2Y9T5S2   A0A3L8S080   A0A1U7TXZ9   G3IGI2   A0A3Q7MJK5   A0A093GRV6   A0A226P183   A0A3L7GLT7   A0A226MWQ9   A0A2U3VW84   A0A2Y9E1J9   A0A2U3XJJ3   A0A341AR24   D3ZGQ5   M3X8S2   A0A3Q7T4X0   Q6DJA8   L5LKK7   A0A2U4ANQ4   A0A2K5DQ04   A0A2Y9JXW2   A0A1S3A7U2   A0A2Y9I7H5   A0A1W4ZSK4   A0A3P4M9Y8   G3TB80   M3YRU0   A0A2K6FSD9   A0A3Q7Y6N9   G3ULQ9   A0A2U4ANN3   G1QRK6   A0A096P3C4   A0A1W4ZJ40   E1B827   A0A2K6DDI2   A0A2K5XGK4   A0A2K5NPU5   G7NGK7   G7PTY4   A0A2K6P0B4   F6UM89   A0A2Y9Q216   W5P0W7   Q86SG6   A0A1L8H516   A0A093GNQ0   A0A091D5M3   G5BVI5   S7NAL6   Q91ZR4   A0A2K6LPL6   K1Q884   G9KD28   A0A2R8ZBY5   H2QCJ1   A0A1U7QY18   G1NW63   G1SE82   H0X674   A0A2J8TMR5   A0A2K6T597   H0UW90   F6YW35   A0A212D7H0   A0A093IYN3   A0A2Y9FFJ8   I3L8I2   A0A0P6J5G7   A0A341AUP5   A0A384A9J9   A0A2U4ANQ0  

EC Number

2.7.11.1

Pubmed

22118469    26354079    26227816    28004739    16341006    20838655    + More
16625196    18563158    20360741    23371554    15592404    30282656    21804562    24621616    29704459    15057822    23793029    17975172    19393038    22002653    25362486    17431167    20809919    15489334    17974005    15019993    23418306    18199800    27762356    21993625    12421721    16141072    16267153    18235101    25671767    22992520    23236062    22722832    16136131    21993624    19892987   

EMBL

AGBW02010868    OWR47598.1    NWSH01003294    PCG66601.1    KQ459586    KPI97953.1    + More
RSAL01000040    RVE50949.1    KQ460883    KPJ11327.1    JTDY01001687    KOB73154.1    JTDY01010679    KOB58103.1    GEZM01024956    JAV87675.1    AAEX03006669    GCES01032070    JAR54253.1    NBAG03000289    PNI46994.1    AC010761    NDHI03003489    PNJ34275.1    GG666631    EEN47471.1    QRBI01000111    RMC10720.1    AK301429    BAG62957.1    ABQF01026366    LSYS01005108    OPJ78619.1    MUZQ01000070    OWK59905.1    AKCR02000235    PKK18046.1    AADN05000057    QUSF01000101    RLV92295.1    JH002576    EGW14552.1    KL216427    KFV69529.1    AWGT02000220    OXB73372.1    RAZU01001831    RLQ54628.1    MCFN01000384    OXB59540.1    AABR06064754    CH473948    AANG04004035    BC075274    AAH75274.1    KB110620    ELK26939.1    CYRY02007629    VCW76608.1    AEYP01021287    AEYP01021288    ADFV01099414    ADFV01099415    ADFV01099416    ADFV01099417    AHZZ02009949    CM001268    EHH24644.1    AQIA01027020    CM001291    EHH57833.1    JSUE03016317    AMGL01017759    AMGL01017760    AY242354    AY267371    CH471159    BC112240    BC113705    AL833909    CM004469    OCT91101.1    KL205569    KFV71933.1    KN123351    KFO25565.1    JH172092    EHB13321.1    KE163905    EPQ14334.1    AF407579    AK154358    BC070457    JH818702    EKC30153.1    JP014205    AES02803.1    AJFE02040461    AACZ04041502    PNI46993.1    AAPE02030648    AAGW02049845    AAQR03132776    AAQR03132777    AAQR03132778    PNJ34274.1    AAKN02033340    MKHE01000005    OWK14199.1    KK569907    KFW06778.1    AEMK02000080    GEBF01004791    JAN98841.1   

Proteomes

UP000007151    UP000218220    UP000053268    UP000283053    UP000053240    UP000037510    + More
UP000002254    UP000001645    UP000005640    UP000001554    UP000269221    UP000007754    UP000085678    UP000081671    UP000190648    UP000197619    UP000053872    UP000261680    UP000000539    UP000248484    UP000276834    UP000189704    UP000001075    UP000286641    UP000053875    UP000198419    UP000273346    UP000198323    UP000245340    UP000248480    UP000245341    UP000252040    UP000002494    UP000011712    UP000286640    UP000245320    UP000233020    UP000248482    UP000079721    UP000248481    UP000192224    UP000007646    UP000000715    UP000233160    UP000286642    UP000001073    UP000028761    UP000009136    UP000233120    UP000233140    UP000233060    UP000009130    UP000233100    UP000233200    UP000006718    UP000248483    UP000002356    UP000186698    UP000053584    UP000028990    UP000006813    UP000000589    UP000233180    UP000005408    UP000240080    UP000002277    UP000189706    UP000001074    UP000001811    UP000005225    UP000233220    UP000005447    UP000002281    UP000008227    UP000261681   

PRIDE

K7EL04    B4DW95    G3IGI2    D3ZGQ5    Q86SG6    Q91ZR4    + More
I3L8I2   

Pfam

PF00069   Pkinase        + More
PF00415   RCC1
PF02176   zf-TRAF

Interpro

IPR017441   Protein_kinase_ATP_BS        + More
IPR008271   Ser/Thr_kinase_AS       
IPR001245   Ser-Thr/Tyr_kinase_cat_dom       
IPR011009   Kinase-like_dom_sf       
IPR000719   Prot_kinase_dom       
IPR000408   Reg_chr_condens       
IPR009091   RCC1/BLIP-II       
IPR001293   Znf_TRAF       
IPR013083   Znf_RING/FYVE/PHD       
IPR037307   TRAF4_MATH       
IPR008974   TRAF-like       
IPR002083   MATH/TRAF_dom       

SUPFAM

SSF56112   SSF56112        + More
SSF50985   SSF50985       
SSF49599   SSF49599       

Gene 3D

2.130.10.30     2.60.210.10     3.30.40.10    

CDD

cd03781  MATH_TRAF4

ProteinModelPortal

A0A212F1I3   A0A2A4J3N5   A0A194PY50   A0A3S2LCX9   A0A194R0U8   A0A0L7LCN4   + More
A0A0L7K4C3   A0A1Y1MT33   J9NYR9   H9H1W0   A0A146YJB5   A0A2J8LIA4   K7EL04   A0A2J8TMM9   C3ZJ42   A0A3M0KIA4   B4DW95   H0Z713   A0A1S3H2T7   A0A1S3F541   A0A2R2MJK9   A0A1V4K2E2   A0A218V1J8   A0A2I0LKT7   A0A384CH41   E1BRZ2   A0A2Y9T5S2   A0A3L8S080   A0A1U7TXZ9   G3IGI2   A0A3Q7MJK5   A0A093GRV6   A0A226P183   A0A3L7GLT7   A0A226MWQ9   A0A2U3VW84   A0A2Y9E1J9   A0A2U3XJJ3   A0A341AR24   D3ZGQ5   M3X8S2   A0A3Q7T4X0   Q6DJA8   L5LKK7   A0A2U4ANQ4   A0A2K5DQ04   A0A2Y9JXW2   A0A1S3A7U2   A0A2Y9I7H5   A0A1W4ZSK4   A0A3P4M9Y8   G3TB80   M3YRU0   A0A2K6FSD9   A0A3Q7Y6N9   G3ULQ9   A0A2U4ANN3   G1QRK6   A0A096P3C4   A0A1W4ZJ40   E1B827   A0A2K6DDI2   A0A2K5XGK4   A0A2K5NPU5   G7NGK7   G7PTY4   A0A2K6P0B4   F6UM89   A0A2Y9Q216   W5P0W7   Q86SG6   A0A1L8H516   A0A093GNQ0   A0A091D5M3   G5BVI5   S7NAL6   Q91ZR4   A0A2K6LPL6   K1Q884   G9KD28   A0A2R8ZBY5   H2QCJ1   A0A1U7QY18   G1NW63   G1SE82   H0X674   A0A2J8TMR5   A0A2K6T597   H0UW90   F6YW35   A0A212D7H0   A0A093IYN3   A0A2Y9FFJ8   I3L8I2   A0A0P6J5G7   A0A341AUP5   A0A384A9J9   A0A2U4ANQ0  

PDB

4B9D     E-value=1.08629e-48,     Score=486

KEGG

101746358    K20877 NIMA (never in mitosis gene a)-related kinase 8 [EC:2.7.11.1] | (RefSeq) serine/threonine-pro

GO

GO:0005524   GO:0004674   GO:0004672   GO:0009887   GO:0005929   GO:0035330   GO:0060271   GO:0007507   GO:0097546   GO:0097543   GO:0007368   GO:0016301   GO:0005737   GO:0046872   GO:0005856   GO:0008270   GO:0046330   GO:0007250   GO:0006468   GO:0003824   GO:0016829   GO:0048869   GO:0003676   GO:0016491   GO:0015930  

Subcellular location

Cytoplasm   Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells (By similarity).   With evidence from 1 publications.
Cytoskeleton   Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells (By similarity).   With evidence from 1 publications.
Cell projection   Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells (By similarity).   With evidence from 1 publications.
Cilium   Predominantly cytoplasmic. Localizes to the proximal region of the primary cilium and is not observed in dividing cells (By similarity).   With evidence from 1 publications.

Length:

293

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

2.22931

Exp number, first 60 AAs:

0.00362

Total prob of N-in:

0.17294

outside

1  -  293

 

 

Pi

231.523973

Theta

162.797981

Tajima's D

2.670674

CLR

0

CSRT

0.95865206739663

Interpretation

Uncertain