Gene

KWMTBOMO13041  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA000292

Annotation

dihydrolipoamide_dehydrogenase_[Bombyx_mori]

Location

Bomo_Chr22(+):7041002-7049730      View in EpiBrowser

Full name

Dihydrolipoyl dehydrogenase       + More
Dihydrolipoyl dehydrogenase, mitochondrial      

Alternative Name

Dihydrolipoamide dehydrogenase
E3

Location in the cell

Cytoplasmic   Reliability : 1.783 Mitochondrial   Reliability : 2.428

CDS

ATGGGCTATAAATTTCTAAAACTTGCCTCTCCTACCTTTAGAAGTGGAAGTTTAGTACGAATTGCTACAAGACAATATGCTACCACACATGATGCAGATCTTGTTGTTATCGGTTCGGGCCCTGGTGGATACGTAGCAGCTATTAAAGCTGCCCAGCTTGGCATGAAGGTGGTCTCAGTAGAAAAGGACCCTACTTTAGGAGGTACTTGTCTCAATGTTGGATGTATACCATCAAAAGCTTTACTGCACAACTCACATCTTTACCATATGGCCAAACATGACTTCAAGCAAAGGGGTATTGAAACTGGTGAAGTTACATTTGACTTCAAAAAGATGATGGAATATAAAGCTAATGCTGTTAAAGGCCTCACAGGAGGTATTGCAATGCTCTTTCAGAAGAACAAGGTTAATCTTGTCAAAGGTGTAGGAACTATAGTTGCCCCAAATAAAGTTGAAGTACACGGAGAGAAGGGTGTTGAGACTGTTAATACCAAAAATATTCTAATCGCTTCTGGGTCTGAAGTTACACCTTTCCCTGGAGTTACTTTTGATGAGAAACAAATAATCACATCGACTGGAGCACTTTCCTTGGAGTCAGTACCGAAAAAAATGTTGGTGATTGGAGCTGGAGTTATCGGTTTGGAGCTTGGTTCTGTTTATCAAAGACTTGGTGCTGATGTTACTGCAATTGAATTCCTAACTAGCATTGGAGGTGTTGGTATTGATGGAGAGGTAGCCAAGACTCTTCAGAAAATCTTAAGCAAACAAGGAATGAAATTCAAACTGGGAACTAAAGTTTTAGGGGTTAAGAAAGAAGGTAGTACTATCAAAGTTGATGTTGAAGCTGCTAAAGGTGGCAATAAGGAAGTGTTGGATTGCGATGTGGTGCTAATTTCAATTGGTCGTCGCCCTTACACTAAAGGTCTCGGGCTCGACAAAGTGGGCATCGCTTTGGACGACCGCGGCCGGATTCCTGTCAACAACAAATTCCAAACTACTGTGCCCGGAATTTACGCTATTGGTGATGTAATTCACGGACCAATGTTGGCTCACAAAGCGGAGGACGAAGGTATCGTCTGCGTGGAAGGTATTAAGGGTATGCCCGTCCACTTCAACTACGATGCAATTCCGTCGGTTATTTACACTTCACCCGAAGTTGGCTGGGTCGGAAAGACTGAAGAAGATTTAAAGAAAGAAGGCAGAGCTTACAAAGTGGGTAAATTCCCGTTCCTGGCGAACTCCAGAGCGAAAACGAATGGGGAAACTGAAGGTTTCGTCAAAGTGCTTTCCGATAAGACTACAGACGTAATCCTTGGGACTCACATTATTGGCCCTGGCGGTGGTGAGTTGATCAACGAGGCTGTACTCGCGCAGGAGTATGGTGCCGCTGCTGAAGACGTTGCGAGAGTCTGTCACGCGCATCCTACATGTGCCGAAGCGCTACGTGAAGCAAATCTAGCAGCGTACTCCGGAAAACCAATCAACTTCTAA

Protein

MGYKFLKLASPTFRSGSLVRIATRQYATTHDADLVVIGSGPGGYVAAIKAAQLGMKVVSVEKDPTLGGTCLNVGCIPSKALLHNSHLYHMAKHDFKQRGIETGEVTFDFKKMMEYKANAVKGLTGGIAMLFQKNKVNLVKGVGTIVAPNKVEVHGEKGVETVNTKNILIASGSEVTPFPGVTFDEKQIITSTGALSLESVPKKMLVIGAGVIGLELGSVYQRLGADVTAIEFLTSIGGVGIDGEVAKTLQKILSKQGMKFKLGTKVLGVKKEGSTIKVDVEAAKGGNKEVLDCDVVLISIGRRPYTKGLGLDKVGIALDDRGRIPVNNKFQTTVPGIYAIGDVIHGPMLAHKAEDEGIVCVEGIKGMPVHFNYDAIPSVIYTSPEVGWVGKTEEDLKKEGRAYKVGKFPFLANSRAKTNGETEGFVKVLSDKTTDVILGTHIIGPGGGELINEAVLAQEYGAAAEDVARVCHAHPTCAEALREANLAAYSGKPINF

Catalytic Activity

(R)-N(6)-dihydrolipoyl-L-lysyl-[protein] + NAD(+) = (R)-N(6)-lipoyl-L-lysyl-[protein] + H(+) + NADH

Cofactor

FAD

Subunit

Homodimer.

Miscellaneous

The active site is a redox-active disulfide bond.

Similarity

Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family.

Keywords

Cytoplasm   Disulfide bond   FAD   Flavoprotein   Glycolysis   NAD   Oxidoreductase   Redox-active center   Alternative splicing   Complete proteome   Fatty acid biosynthesis   Fatty acid metabolism   Lipid biosynthesis   Lipid metabolism   Mitochondrion   Reference proteome   Transit peptide  

Feature

chain  Dihydrolipoyl dehydrogenase
splice variant  In isoform b.

Uniprot

Q8MUB0   D6R773   I4DMH5   S4P991   O18480   A0A3S2M9V9   + More
A0A212FM25   A0A2H1VCT0   A0A2W1C0K5   A0A194Q332   I4DJF2   A0A2A4IX85   A0A0L0CMD7   B4L0F4   W8C1K5   T1PHL2   A0A1I8NPA9   A0A0K8TZ55   A0A034VK02   A0A0A1XNC7   B4LIF2   D3TRU9   A0A1A9URY6   A0A1L8EEI8   Q9VVL7   K7PQ56   B3M3U9   A0A1B0A6P9   A0A1L6K358   K7PR60   A0A0J9RYP2   B4HLB4   A0A1Y1L6U1   A0A1W4W542   K7PQS7   B4PJH9   A0A0M5JB65   K7PQU4   B4N4T3   A0A1L6K360   K7PQU5   K7PQ54   B4QPB7   A0A1B0AW18   K7PQ78   A0A0F6QCS7   B4J058   B3NDC3   A0A0K8TPK2   K7PR58   A0A1A9Y261   Q2LZ16   B4GZR7   K7PR59   A0A1B0CS16   A0A3B0J420   A0A1L8E562   K7J6V1   A0A0F6QB24   E2BDQ8   A0A336LRF6   A0A0U4JS04   G0N6W0   O17953   E3N753   A0A261B5H6   A0A0J7KJN3   A0A1I7UV22   E1ZX53   A0A0U4IDU2   A0A2G5TXX6   A0A2P2HXL3   A8Y0F0   N6T3X8   E9IVN8   A0A0U4AV64   A0A195FWE8   A0A195EE33   A0A151XA98   K7PQ79   A0A1B6HDG4   A0A0F6QE70   A0A195CT09   F4WLJ5   A0A0A9XAA8   A0A026WWC0   A0A0F6QCA3   A0A1B6LXD2   A0A232EGN4   A0A0N7Z9G7   R4G4V3   A0A1S6KZB2   A0A1J1HUU2   A0A226E0Z4   A0A3P8F2V0   A0A2H2IN90   A0A1D2MWM9   A0A069DVF9   A0A310SQX7   A0A0L7RD32  

EC Number

1.8.1.4

Pubmed

19121390    20093189    22651552    23622113    9373151    22118469    + More
28756777    26354079    26108605    17994087    24495485    25315136    25348373    25830018    20353571    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    23139334    22936249    28004739    17550304    18057021    24059691    25853517    26369729    15632085    20075255    20798317    26774057    9851916    14624247    23537049    21282665    21719571    25401762    26823975    24508170    30249741    28648823    27129103    24330026    27289101    26334808   

EMBL

BABH01013298    BABH01013299    AF529135    AAM93255.1    HM126490    ADG45566.1    + More
AK402493    BAM19115.1    GAIX01003839    JAA88721.1    AF008586    RSAL01000005    RVE54366.1    AGBW02007691    OWR54739.1    ODYU01001851    SOQ38627.1    KZ149892    PZC79027.1    KQ459586    KPI97815.1    AK401420    BAM18042.1    NWSH01005242    PCG64341.1    JRES01000296    KNC32634.1    CH933809    EDW19123.1    GAMC01003517    JAC03039.1    KA647378    AFP62007.1    GDHF01032746    JAI19568.1    GAKP01016148    JAC42804.1    GBXI01001766    JAD12526.1    CH940647    EDW70739.1    CCAG010008063    EZ424151    ADD20427.1    GFDG01001780    JAV17019.1    AE014296    AY058740    AAF49294.1    AAL13969.1    JX434602    AFP20528.1    CH902618    EDV39283.1    KX774473    KX774474    KX774475    KX774476    KX774477    KX774479    APR62612.1    APR62613.1    APR62614.1    APR62615.1    APR62616.1    APR62618.1    JX434596    JX434608    AFP20534.1    CM002912    KMZ00325.1    CH480815    EDW41934.1    GEZM01065180    GEZM01065179    JAV68538.1    JX434601    AFP20527.1    CM000159    EDW94667.1    CP012525    ALC43868.1    JX434600    MH891063    MH891065    MH891066    AFP20526.1    QBH99336.1    QBH99338.1    QBH99339.1    CH964095    EDW79157.1    KRF99016.1    KX774472    KX774478    APR62611.1    APR62617.1    JX434605    KF032715    AFP20531.1    AGX84981.1    JX434597    MH891069    AFP20523.1    QBH99342.1    CM000363    EDX10911.1    JXJN01004447    JX434599    AFP20525.1    KP843188    AKD42940.1    CH916366    EDV97851.1    CH954178    EDV51916.1    GDAI01001783    JAI15820.1    JX434598    AFP20524.1    CH379069    EAL29693.1    CH479199    EDW29494.1    JX434603    AFP20529.1    AJWK01025561    AJWK01025562    OUUW01000002    SPP76494.1    GFDF01000412    JAV13672.1    AAZX01011402    AAZX01011404    AAZX01017942    KP843190    AKD42942.1    GL447678    EFN86220.1    UFQT01000131    SSX20616.1    KT893322    KT893323    ALY05704.1    ALY05705.1    GL379845    EGT53974.1    Z82277    DS268545    EFO88408.1    NIPN01000162    OZG05582.1    LBMM01006632    KMQ90446.1    GL435014    EFN74243.1    KT893325    KT893326    KT893327    ALY05707.1    ALY05708.1    ALY05709.1    PDUG01000004    PIC31951.1    IACF01000746    LAB66502.1    HE601157    CAP38335.2    APGK01053269    APGK01053270    KB741223    KB632273    ENN72338.1    ERL91063.1    GL766328    EFZ15353.1    KT893324    ALY05706.1    KQ981208    KYN44751.1    KQ979039    KYN23366.1    KQ982351    KYQ57269.1    JX434604    AFP20530.1    GECU01034976    JAS72730.1    KP843191    AKD42943.1    KQ977372    KYN03264.1    GL888208    EGI64931.1    GBHO01027038    GBRD01015774    GDHC01009946    JAG16566.1    JAG50052.1    JAQ08683.1    KK107085    QOIP01000008    EZA60031.1    RLU19442.1    KP843189    AKD42941.1    GEBQ01011628    JAT28349.1    NNAY01004725    OXU17511.1    GDKW01000595    JAI56000.1    ACPB03011520    GAHY01000431    JAA77079.1    KC223159    AQT27062.1    CVRI01000021    CRK91839.1    LNIX01000008    OXA51139.1    UZAH01031137    VDP14026.1    LJIJ01000451    ODM97358.1    GBGD01001192    JAC87697.1    KQ760869    OAD58780.1    KQ414614    KOC68872.1   

Proteomes

UP000005204    UP000283053    UP000007151    UP000053268    UP000218220    UP000037069    + More
UP000009192    UP000095301    UP000095300    UP000008792    UP000092444    UP000078200    UP000000803    UP000007801    UP000092445    UP000001292    UP000192221    UP000002282    UP000092553    UP000007798    UP000000304    UP000092460    UP000001070    UP000008711    UP000092443    UP000001819    UP000008744    UP000092461    UP000268350    UP000002358    UP000008237    UP000008068    UP000001940    UP000008281    UP000216463    UP000036403    UP000095282    UP000000311    UP000230233    UP000008549    UP000019118    UP000030742    UP000078541    UP000078492    UP000075809    UP000078542    UP000007755    UP000053097    UP000279307    UP000215335    UP000015103    UP000183832    UP000198287    UP000005237    UP000094527    UP000053825   

PRIDE

Q8MUB0    D6R773    O18480    Q9VVL7    O17953   

Pfam

PF07992   Pyr_redox_2        + More
PF02852   Pyr_redox_dim

Interpro

IPR036188   FAD/NAD-bd_sf        + More
IPR012999   Pyr_OxRdtase_I_AS       
IPR016156   FAD/NAD-linked_Rdtase_dimer_sf       
IPR023753   FAD/NAD-binding_dom       
IPR001100   Pyr_nuc-diS_OxRdtase       
IPR004099   Pyr_nucl-diS_OxRdtase_dimer       
IPR006258   Lipoamide_DH       

SUPFAM

SSF55424   SSF55424        + More
SSF51905   SSF51905       

Gene 3D

3.30.390.30     3.50.50.60    

ProteinModelPortal

Q8MUB0   D6R773   I4DMH5   S4P991   O18480   A0A3S2M9V9   + More
A0A212FM25   A0A2H1VCT0   A0A2W1C0K5   A0A194Q332   I4DJF2   A0A2A4IX85   A0A0L0CMD7   B4L0F4   W8C1K5   T1PHL2   A0A1I8NPA9   A0A0K8TZ55   A0A034VK02   A0A0A1XNC7   B4LIF2   D3TRU9   A0A1A9URY6   A0A1L8EEI8   Q9VVL7   K7PQ56   B3M3U9   A0A1B0A6P9   A0A1L6K358   K7PR60   A0A0J9RYP2   B4HLB4   A0A1Y1L6U1   A0A1W4W542   K7PQS7   B4PJH9   A0A0M5JB65   K7PQU4   B4N4T3   A0A1L6K360   K7PQU5   K7PQ54   B4QPB7   A0A1B0AW18   K7PQ78   A0A0F6QCS7   B4J058   B3NDC3   A0A0K8TPK2   K7PR58   A0A1A9Y261   Q2LZ16   B4GZR7   K7PR59   A0A1B0CS16   A0A3B0J420   A0A1L8E562   K7J6V1   A0A0F6QB24   E2BDQ8   A0A336LRF6   A0A0U4JS04   G0N6W0   O17953   E3N753   A0A261B5H6   A0A0J7KJN3   A0A1I7UV22   E1ZX53   A0A0U4IDU2   A0A2G5TXX6   A0A2P2HXL3   A8Y0F0   N6T3X8   E9IVN8   A0A0U4AV64   A0A195FWE8   A0A195EE33   A0A151XA98   K7PQ79   A0A1B6HDG4   A0A0F6QE70   A0A195CT09   F4WLJ5   A0A0A9XAA8   A0A026WWC0   A0A0F6QCA3   A0A1B6LXD2   A0A232EGN4   A0A0N7Z9G7   R4G4V3   A0A1S6KZB2   A0A1J1HUU2   A0A226E0Z4   A0A3P8F2V0   A0A2H2IN90   A0A1D2MWM9   A0A069DVF9   A0A310SQX7   A0A0L7RD32  

PDB

5NHG     E-value=1.67923e-176,     Score=1591

KEGG

692608    K00382 dihydrolipoamide dehydrogenase [EC:1.8.1.4] | (RefSeq) DLDH; dihydrolipoamide dehydrogenase

PATHWAY

00010    Glycolysis / Gluconeogenesis - Bombyx mori (domestic silkworm)
00020    Citrate cycle (TCA cycle) - Bombyx mori (domestic silkworm)
00260    Glycine, serine and threonine metabolism - Bombyx mori (domestic silkworm)
00280    Valine, leucine and isoleucine degradation - Bombyx mori (domestic silkworm)
00310    Lysine degradation - Bombyx mori (domestic silkworm)
00380    Tryptophan metabolism - Bombyx mori (domestic silkworm)
00620    Pyruvate metabolism - Bombyx mori (domestic silkworm)
00630    Glyoxylate and dicarboxylate metabolism - Bombyx mori (domestic silkworm)
00640    Propanoate metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
01200    Carbon metabolism - Bombyx mori (domestic silkworm)

GO

GO:0050660   GO:0005623   GO:0045454   GO:0009055   GO:0004148   GO:0005739   GO:0005737   GO:0006096   GO:0016668   GO:0005960   GO:0006748   GO:0006099   GO:0005947   GO:0006546   GO:0005759   GO:0006633   GO:0000166   GO:0016491   GO:0005515   GO:0008237   GO:0008152   GO:0015930   GO:0055114  

Subcellular location

Cytoplasm  
Mitochondrion matrix  

Length:

496

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0750400000000001

Exp number, first 60 AAs:

0.06187

Total prob of N-in:

0.00449

outside

1  -  496

 

 

Pi

27.997291

Theta

21.918366

Tajima's D

1.096278

CLR

1.5704

CSRT

0.694065296735163

Interpretation

Uncertain