Gene

KWMTBOMO12970

Pre Gene Modal

BGIBMGA000442

Annotation

PREDICTED:_bifunctional_heparan_sulfate_N-deacetylase/N-sulfotransferase_[Amyelois_transitella]

Location

Bomo_Chr22(-):5933532-5982285      View in EpiBrowser

Full name

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase      

Alternative Name

Glucosaminyl N-deacetylase/N-sulfotransferase
Sulfateless

Location in the cell

Mitochondrial   Reliability : 1.693

CDS

ATGACGGGCGAAAACCGGGGCGCTCGCGCTCCATTACTTGAGTGCGGGAGTGGCGAATACTCGCATAACCATAAGACGTTAGCGCCGCGGTGTTGCTTCTGGTTGGCCAGCCACGTCAACGTCAGAAAATGCGTCGCTGGCGTCATGCTGCTATCCGTCCTCACCATATTGTTTTACTCCTATTATGTCACAATACCTCTGACCAGCTTAGTGTGGCGAGATCGAATCCCGCGTCCACTCACCCAGTGTTCATTGCTCGCTTCGCAACAGCAAGCTGCGCGCGATCATCGTTCGGACGCCCGACTTCGTATCGACGCTAAAGTACTTGTTATAGTAGAATCGGCCTACTCACGACTTGGTCGCGACATCGCCGAGCTCCTTGTCGCCAACCGTATAAGGTACAAAATTGAAGTAGCAGGCAAGAGTCTGCCTGTACTCACCACTTTGGACAAGGGGCGTTACGGCGTGATCGTGTTCGAATCTCTGTCGAAATATGCCAATATGGATAAATGGAATCGAGAGCTACTCGACAAATACTGTCGGGAGTATTCCGTTGGAGTAGTCGGCTTCGCGACGCCTAGCGAAGAGACCTTGGTCGGCGCACAGCTAAAAGGATTTCCACTATTCATGCATACTAATTTGAGGTTGAAGGATGCAACGTTGAACGCTGCTTCACCCGTATTGCGATTGGCTCGTGCTGGAGAAACTGCCTGGGGCGCGCTTCCTGGTGACCACTGGTCGGTGTTCCGAGCCAACAGCTCTACCTACGAACCTATCGCGTGGGCGCTTAGAGAACACGATTATGAAACTGATGGAGAGCGGATGCCGCTGGCAACTGTGATACAAGACCACGGCCGTTTGGACGGCGTCCAACGCGTTCTGTTCGGTAGTGGTCTACAGTTCTGGTTGCATCGACTGCTTTTTCTGGACGCATTAAGCTACCTCAGTCACGGGCAGCTCAGCTTGAGTCTGGATCGATGGATATTGGTTGATATCGATGACATCTTTGTCGGTGAGAAAGGTACCCGTCTTCACGAAGAAGACGTGGCGGCACTATTGACGTCTCAGGCAGCACTGCAGCGATTAGTTCCGGGATTCAGGTTCAACCTCGGCTACAGCGCCAAGTACTACCACCACGGAACGCCAACGGAAAACCTCGGCGATGACGCTCTGTTAAAACATAGGGAATATTTTAATTGGTTTTGCCACATGTGGAACCATCAACAGCCTCACCTGTACAACAACGTGTCTCAGCTCGAAGCGGAAATGATGCTGAACAAACAGTTCGCTCTAGAGCACGGGATCCCGACGAACTCGTGCTACTCTGTGTCCCCGCACCACTCCGGAGTCTATCCCGTACACGAACCTCTATACGAAGCCTGGAGGAAAGTCTGGGACGTTAAAGTTACTAGCACGGAGGAGTATCCTCACTTAAGACCAGCTAGATTACGTCGCGGTTTCCGGCACCGGGGCGTGATGGTGTTGCCGCGTCAAACGTGCGGTCTGTTCACGCACACGTTACTGTTGGAGCGTTATCCGGGCGGGAGGCATCGACTAGACCGCTCCATACAGGGAGGCGAGCTCTTCCAGACTGTTATCAACAATCCGATCAACGTGTTCATGACCCACATGTCGAATTATGGCAACGATCGACTCGCATTATACACTTTCGAGTCGGTGGTCAAGTTCCTGAGATGCTGGACCAATGTGCGTCTTGCATCAGCACCACCTTTGGCCCTGGCGGAGAAGTATTTCCAACTGAGACCCGACGAACTGAACCCGTTGTGGGGGAATCCGTGCGATGACATCCGACACCGCCGCATATGGTCCAAGAGTAAATGGTGCGGCACACTGCCCAGACTCCTAGTGATCGGTCCCCAGAAGACCGGCAGCACAGCCCTATACACGTTCCTAGCGATGCACCCAACACTGAAGCCGAACTTGCCCAGCCCGAGCACATACGAAGAGCTACAATTCTTCAATAACAACAACTACCTTAAAGGACTGGATTGGTACCTGAATTTTTTCCCGCCAAGTCTAACTAACAACTCACAAATAACATTCGAGAAGTCGGCGACGTACTTCGACGGCGACCTGGTGCCGAGGAGAGCGCACGCATTACTGCCCAACGCCAAGATCATTGCCATACTGATATCACCATCGAAACGTGCGTACTCCTGGTATCAGCACATACGCTCTCACGGCGACCCGATCGCGAATAATTACACATTCCACACAATCATCACGGCGAATGACTCCGCACCGAAACAACTCAGGGATTTGAGGAATCGTTGCTTGAATCCTGGGAAATACAGTCATTATTTAGAACGTTGGTTGTCGGAGTACAGCGTGCACCAGTTGCACGTCATCGACGGGTCGATGCTGCGCTCTGAGCCGGCGGTCGTGATGAACACGCTCCAGAAGTTCCTCAAGATATCGCCGCATATCGACTACAATAAACTGCTCAAATACGATCCGAAAAAAGGATTCTTCTGTCAAGTGGTCGGGGGCGAGAAAACGAAATGCCTTGGGAAGTCGAAGGGTCGCGTGTACCCGCCGATGGAGGAGCGCTCGGCCAAGTTCCTCAGGCGTTACTACACGCCGCATAACACTGCACTGTCCAAGCTGCTGGTCAGGGTCGGCCGCCCCGTGCCGCACTGGCTCAAGGACGAGCTCTCGGCCAACGGATGA

Protein

MTGENRGARAPLLECGSGEYSHNHKTLAPRCCFWLASHVNVRKCVAGVMLLSVLTILFYSYYVTIPLTSLVWRDRIPRPLTQCSLLASQQQAARDHRSDARLRIDAKVLVIVESAYSRLGRDIAELLVANRIRYKIEVAGKSLPVLTTLDKGRYGVIVFESLSKYANMDKWNRELLDKYCREYSVGVVGFATPSEETLVGAQLKGFPLFMHTNLRLKDATLNAASPVLRLARAGETAWGALPGDHWSVFRANSSTYEPIAWALREHDYETDGERMPLATVIQDHGRLDGVQRVLFGSGLQFWLHRLLFLDALSYLSHGQLSLSLDRWILVDIDDIFVGEKGTRLHEEDVAALLTSQAALQRLVPGFRFNLGYSAKYYHHGTPTENLGDDALLKHREYFNWFCHMWNHQQPHLYNNVSQLEAEMMLNKQFALEHGIPTNSCYSVSPHHSGVYPVHEPLYEAWRKVWDVKVTSTEEYPHLRPARLRRGFRHRGVMVLPRQTCGLFTHTLLLERYPGGRHRLDRSIQGGELFQTVINNPINVFMTHMSNYGNDRLALYTFESVVKFLRCWTNVRLASAPPLALAEKYFQLRPDELNPLWGNPCDDIRHRRIWSKSKWCGTLPRLLVIGPQKTGSTALYTFLAMHPTLKPNLPSPSTYEELQFFNNNNYLKGLDWYLNFFPPSLTNNSQITFEKSATYFDGDLVPRRAHALLPNAKIIAILISPSKRAYSWYQHIRSHGDPIANNYTFHTIITANDSAPKQLRDLRNRCLNPGKYSHYLERWLSEYSVHQLHVIDGSMLRSEPAVVMNTLQKFLKISPHIDYNKLLKYDPKKGFFCQVVGGEKTKCLGKSKGRVYPPMEERSAKFLRRYYTPHNTALSKLLVRVGRPVPHWLKDELSANG

Description

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in diffusion of morphogen wingless (wg) via its role in heparan sulfate proteoglycans (HSPGs) biosynthesis, HSPGs being required for movement of wg morphogens. Required for wg signaling during both embryonic and imaginal disk development. Also required for FGF receptor signaling.

Catalytic Activity

3'-phosphoadenylyl sulfate + alpha-D-glucosaminyl-[heparan sulfate](n) = adenosine 3',5'-bisphosphate + 2 H(+) + N-sulfo-alpha-D-glucosaminyl-[heparan sulfate](n)

Subunit

Monomer.

Similarity

Belongs to the sulfotransferase 1 family. NDST subfamily.

Keywords

Complete proteome   Disulfide bond   Glycoprotein   Golgi apparatus   Hydrolase   Membrane   Multifunctional enzyme   Reference proteome   Signal-anchor   Transferase   Transmembrane   Transmembrane helix   Wnt signaling pathway  

Feature

chain  Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase

Uniprot

A0A2H1X2Y8   A0A212F1B6   A0A194Q817   H9IT63   A0A2J7PPM7   A0A0A1XBX4   + More
A0A1I8MTS9   A0A1B0G391   A0A1I8PNG5   A0A0K8V6K5   A0A0K8WCH7   A0A034V7I9   A0A1J1J5R5   K7IZQ8   W8B640   A0A158NK09   A0A151X1F8   A0A1Q3G0C0   A0A0L0BMA9   B4IXX1   B4LIB0   B3M9Y1   B4KVG2   A0A1W4UX65   B3NGI7   A0A0J9RRK6   A0A0M4E8K9   A0A1Y1MM98   B4PJQ0   E1JID8   Q9V3L1   A0A3L8D8D8   B4N2Z1   Q16LS8   A0A195EUE6   A0A224XEM0   A0A0V0G3I8   A0A3B0KHE6   A0A0A9YCA8   A0A023F4K5   A0A1B6ENZ1   A0A069DWP0   A0A0N7Z962   T1HUA9   A0A0N0BIQ6   A0A195CJT5   A0A1B6L2T5   A0A154PCE4   U5EPY2   B4QJE9   A0A336K7Q8   A0A1B6LXE4   A0A1Y9HDL8   A0A1B0CX87   A0A2Y9D1L4   A0A0T6B084   A0A2M4CU90   A0A2M4CUH9   D6WJ22   Q7Q5P3   A0A084WKB2   A0A1W4WQ95   A0A2M3ZII0   A0A2M4ALR6   A0A2M4BDZ3   A0A1A9V3X6   A0A1A9ZU57   A0A1B0AVQ5   A0A1A9YRV7   A0A195DWC5   A0A195B3X9   B0WMN5   E0VV47   E9I9T1   E2A347   A0A0L7QTA0   A0A182QJ64   A0A182WCE3   A0A182VGG6   A0A182X0U9   A0A182YLE0   A0A182LLB8   Q29FH2   A0A182M6I8   B4H1B3   A0A2S2QAD8   A0A182I102   A0A2M4CZH7   A0A1A9W499   A0A182JI14   A0A182PCB5   A0A182FC56   A0A2H8TF57   A0A2S2NCB4   N6ULE5   A0A182G5V2   X1WI69   A0A0P6IHK9   A0A0P5TQ65   A0A0P5DYY6  

EC Number

2.8.2.8

Pubmed

22118469    26354079    19121390    25830018    25315136    25348373    + More
20075255    24495485    21347285    26108605    17994087    18057021    22936249    28004739    17550304    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    10421372    10433902    10644674    15531366    30249741    17510324    25401762    26823975    25474469    26334808    27129103    18362917    19820115    12364791    24438588    20566863    21282665    20798317    25244985    20966253    15632085    23537049    26483478   

EMBL

ODYU01013031    SOQ59669.1    AGBW02010913    OWR47529.1    KQ459324    KPJ01534.1    + More
BABH01013391    BABH01013392    BABH01013393    BABH01013394    NEVH01022660    PNF18256.1    GBXI01005850    JAD08442.1    CCAG010013733    CCAG010013734    GDHF01017752    JAI34562.1    GDHF01003749    JAI48565.1    GAKP01020851    GAKP01020850    GAKP01020849    JAC38102.1    CVRI01000067    CRL06814.1    GAMC01021266    GAMC01021264    JAB85289.1    ADTU01018584    ADTU01018585    ADTU01018586    ADTU01018587    ADTU01018588    ADTU01018589    ADTU01018590    ADTU01018591    ADTU01018592    KQ982585    KYQ54253.1    GFDL01001815    JAV33230.1    JRES01001668    KNC21063.1    CH916366    EDV96492.1    CH940647    EDW69677.2    CH902618    EDV41201.1    CH933809    EDW18405.2    KRG06179.1    CH954178    EDV51153.2    CM002912    KMY97929.1    KMY97930.1    CP012525    ALC43228.1    GEZM01030073    GEZM01030072    JAV85820.1    CM000159    EDW93649.2    AE014296    BT132640    ACZ94625.1    AEQ05538.1    AF175689    QOIP01000012    RLU16188.1    CH964062    EDW78730.2    CH477892    EAT35284.1    KQ981979    KYN31522.1    GFTR01008198    JAW08228.1    GECL01003486    JAP02638.1    OUUW01000009    SPP85166.1    GBHO01016465    GBHO01016464    GDHC01013977    GDHC01003255    JAG27139.1    JAG27140.1    JAQ04652.1    JAQ15374.1    GBBI01002505    JAC16207.1    GECZ01030134    JAS39635.1    GBGD01000426    JAC88463.1    GDKW01001435    JAI55160.1    ACPB03019114    KQ435727    KOX78012.1    KQ977642    KYN00991.1    GEBQ01022022    JAT17955.1    KQ434870    KZC09599.1    GANO01003521    JAB56350.1    CM000363    EDX09458.1    UFQS01000006    UFQT01000006    SSW97040.1    SSX17427.1    GEBQ01011618    JAT28359.1    AJWK01033418    AJWK01033419    AJWK01033420    AJWK01033421    AJWK01033422    AJWK01033423    AJWK01033424    AJWK01033425    LJIG01016385    KRT80760.1    GGFL01004738    MBW68916.1    GGFL01004737    MBW68915.1    KQ971342    EFA03870.1    AAAB01008960    EAA11447.3    ATLV01024105    KE525349    KFB50656.1    GGFM01007497    MBW28248.1    GGFK01008412    MBW41733.1    GGFJ01002040    MBW51181.1    JXJN01004361    KQ980295    KYN16864.1    KQ976625    KYM78977.1    DS231999    EDS31117.1    DS235802    EEB17253.1    GL761846    EFZ22726.1    GL436272    EFN72150.1    KQ414755    KOC61799.1    AXCN02000408    CH379067    EAL31283.3    AXCM01012933    CH479202    EDW30090.1    GGMS01005500    MBY74703.1    APCN01000007    GGFL01006453    MBW70631.1    GFXV01000928    GFXV01003391    GFXV01003852    MBW12733.1    MBW15196.1    MBW15657.1    GGMR01002201    MBY14820.1    APGK01018841    KB740085    KB631844    ENN81496.1    ERL86671.1    JXUM01044009    KQ561384    KXJ78757.1    ABLF02022314    ABLF02022316    ABLF02022317    ABLF02050851    GDIQ01005920    JAN88817.1    GDIP01126169    JAL77545.1    GDIP01150370    JAJ73032.1   

Proteomes

UP000007151    UP000053268    UP000005204    UP000235965    UP000095301    UP000092444    + More
UP000095300    UP000183832    UP000002358    UP000005205    UP000075809    UP000037069    UP000001070    UP000008792    UP000007801    UP000009192    UP000192221    UP000008711    UP000092553    UP000002282    UP000000803    UP000279307    UP000007798    UP000008820    UP000078541    UP000268350    UP000015103    UP000053105    UP000078542    UP000076502    UP000000304    UP000075900    UP000092461    UP000075884    UP000007266    UP000007062    UP000030765    UP000192223    UP000078200    UP000092445    UP000092460    UP000092443    UP000078492    UP000078540    UP000002320    UP000009046    UP000000311    UP000053825    UP000075886    UP000075920    UP000075903    UP000076407    UP000076408    UP000075882    UP000001819    UP000075883    UP000008744    UP000075840    UP000091820    UP000075880    UP000075885    UP000069272    UP000019118    UP000030742    UP000069940    UP000249989    UP000007819   

PRIDE

H9IT63    E1JID8    Q9V3L1   

Pfam

PF12062   HSNSD        + More
PF00685   Sulfotransfer_1

Interpro

IPR021930   Heparan_SO4_deacetylase        + More
IPR000863   Sulfotransferase_dom       
IPR037359   NST/OST       
IPR027417   P-loop_NTPase       

SUPFAM

SSF52540   SSF52540       

ProteinModelPortal

A0A2H1X2Y8   A0A212F1B6   A0A194Q817   H9IT63   A0A2J7PPM7   A0A0A1XBX4   + More
A0A1I8MTS9   A0A1B0G391   A0A1I8PNG5   A0A0K8V6K5   A0A0K8WCH7   A0A034V7I9   A0A1J1J5R5   K7IZQ8   W8B640   A0A158NK09   A0A151X1F8   A0A1Q3G0C0   A0A0L0BMA9   B4IXX1   B4LIB0   B3M9Y1   B4KVG2   A0A1W4UX65   B3NGI7   A0A0J9RRK6   A0A0M4E8K9   A0A1Y1MM98   B4PJQ0   E1JID8   Q9V3L1   A0A3L8D8D8   B4N2Z1   Q16LS8   A0A195EUE6   A0A224XEM0   A0A0V0G3I8   A0A3B0KHE6   A0A0A9YCA8   A0A023F4K5   A0A1B6ENZ1   A0A069DWP0   A0A0N7Z962   T1HUA9   A0A0N0BIQ6   A0A195CJT5   A0A1B6L2T5   A0A154PCE4   U5EPY2   B4QJE9   A0A336K7Q8   A0A1B6LXE4   A0A1Y9HDL8   A0A1B0CX87   A0A2Y9D1L4   A0A0T6B084   A0A2M4CU90   A0A2M4CUH9   D6WJ22   Q7Q5P3   A0A084WKB2   A0A1W4WQ95   A0A2M3ZII0   A0A2M4ALR6   A0A2M4BDZ3   A0A1A9V3X6   A0A1A9ZU57   A0A1B0AVQ5   A0A1A9YRV7   A0A195DWC5   A0A195B3X9   B0WMN5   E0VV47   E9I9T1   E2A347   A0A0L7QTA0   A0A182QJ64   A0A182WCE3   A0A182VGG6   A0A182X0U9   A0A182YLE0   A0A182LLB8   Q29FH2   A0A182M6I8   B4H1B3   A0A2S2QAD8   A0A182I102   A0A2M4CZH7   A0A1A9W499   A0A182JI14   A0A182PCB5   A0A182FC56   A0A2H8TF57   A0A2S2NCB4   N6ULE5   A0A182G5V2   X1WI69   A0A0P6IHK9   A0A0P5TQ65   A0A0P5DYY6  

PDB

1NST     E-value=8.37458e-113,     Score=1044

KEGG

101743047    K02577 heparan sulfate N-deacetylase/N-sulfotransferase NDST2 [EC:3.1.1.- 2.8.2.-] | (RefSeq) bifunc

PATHWAY

00534    Glycosaminoglycan biosynthesis - heparan sulfate / heparin - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)

GO

GO:0016021   GO:0015016   GO:0016787   GO:0045570   GO:0008543   GO:0006024   GO:0016055   GO:0007474   GO:0006790   GO:0008587   GO:0007283   GO:0007427   GO:0015014   GO:0007367   GO:0005783   GO:0000137   GO:0007509   GO:0030210   GO:0008146   GO:0000139   GO:0015012   GO:0034483   GO:0019213   GO:0042328   GO:0005794   GO:0043565   GO:0007155   GO:0005515   GO:0008237   GO:0008152   GO:0016491   GO:0015930  

PANTHER

PTHR10605  

Subcellular location

Golgi apparatus membrane  

Length:

896

Number of predicted TMHs:

1

Exp number of AAs in TMHs:

22.40905

Exp number, first 60 AAs:

13.81826

Total prob of N-in:

0.99792

POSSIBLE N-term signal

sequence

inside

1  -  49

TMhelix

50  -  72

outside

73  -  896

 

 

Pi

244.449442

Theta

175.460122

Tajima's D

1.142705

CLR

0.299649

CSRT

0.697315134243288

Interpretation

Uncertain