Gene

KWMTBOMO12965  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA000447

Annotation

N-ethylmaleimide_sensitive_fusion_protein_[Danaus_plexippus]

Location

Bomo_Chr22(-):5792388-5810994      View in EpiBrowser

Full name

Vesicle-fusing ATPase 2      

Alternative Name

N-ethylmaleimide-sensitive fusion protein 2
Vesicular-fusion protein NSF2
dNsf-2

Location in the cell

Nuclear   Reliability : 2.427

CDS

ATGTCTTCAATGCGAATGAAAGCAGCCAAATGTCCATCTGATGAGCTTGCCATCACCAACTGTGCTCTAATACATCAAGATGACTTTCCAAGTGACATCAAGCATATTGAAGTATCTACAGGACCATCTCAACACTTTGTGTTCAGCATTCGGTTCTACAATGGAGTTGACCGTGGCACTGTTGGATTTTCTGCCCCTCAGCGAAAATGGGCTACCCTCTCTATCGGTCAAACCATTGACGTGAAGCCTTTCAAACCCTCTAGTGCTGAATGCCTTTGCAGCGTAACGTTGGAAGCCGATTTCATGATGAAGAAAACAACATCAACGGATCCATATGATTCGGAACAAATGGCCAGAGATTTCCTTATTCAGTTCGCCAATCAAGTTTTTACTGTCGGACAACAGCTGGCTTTCTTGTTCCAAGAGAAGAAAGTCTTGTCGTTGATCGTAAAAAATTTGGAAGCCGTCGATGTGCAAGCTTTAGCGGCCGGAGCCAACGCCGTGCCCCGCCGGGTGCGGATGGGCCGGCTGCTGCCCGACGCCTCGGTGCAGTTCGACAAGGCGGAGAATTCGTCTCTGAACCTAATCGGGAAGGCTAAAGGAAAGCAACCGCGACAGTCGATCATCAATCCGGACTGGGACTTCGGCAAGATGGGCATCGGCGGTCTCGACAACGAGTTCAACGCGATCTTCCGTCGCGCGTTTGCTTCCCGAGTGTTCCCCCCGGAGGTCGTCGAGCAACTAGGCTGCAAGCACGTGAAGGGAATCTTGCTCTACGGTCCGCCCGGCACCGGCAAGACTTTGATGGCCCGACAGATCGGGAAGATGCTAAACGCCCGCGAACCGAAGATCGTCAACGGCCCACAGATATTGGACAAATACGTTGGCGAGAGCGAGGCCAACATTCGACGTTTATTTGCCGACGCCGAGGAAGAAGAGAAGCGATGCGGAGCGAACAGCGGGCTTCACATCATAATATTCGATGAAATCGACGCGATCTGCAAGGCCAGGGGCTCCGTCGGCGGCAACACCGGCGTCCACGACACCGTCGTCAATCAGCTCCTCTCCAAGATCGACGGTGTGGATCAACTGAACAATATCCTGGTGATCGGTATGACGAACCGGCGCGACATGATCGACGAGGCCCTGCTCCGACCCGGCCGTCTCGAGGTGCAGATGGAGATCGGGCTTCCCGACGAGAAGGGGCGCGTCCAGATATTGAACATTCACACCAAGCGCATGCGCGAGTATAAGAAAATCGCCGAAGACGTCGACTCTATGGAGTTGGCAACGTTAACGAAGAACTTCTCTGGGGCCGAACTGGAGGGTTTGGTCCGAGCTGCCCAGTCGACCGCCATGAACAGACTCATCAAGGCGTCGAGCAAAGTCGAAGTCGATCCCGAAGCCATGGAGAAACTGATGGTCGAACGTACCGACTTCTTGCACGCCTTGGAGAATGATATCAAACCGGCCTTCGGTACATCCGCCGAAGCGCTCGAGCACTTCTTGGCTCGCGGCATCGTCAACTGGGGCTCTCCTGTTTCTTCTATATTTGAAGATGGACAGTTGTATATTCAACAGGCGCGGGCGACCGAAGCCAGCGGTCTGGTGTCGGTGCTGTTGGAGGGCCCCCCGAACAGCGGCAAGACGGCCCTCGCCGCGCAACTGGCCAAGCTGTCGGACTTCCCGTTCGTGAAGGTTTGCTCGCCCGAAGACATGGTCGGCTTCACGGAGACAGCTAAATGCTTGCAGATTAGGAAGTATTTCGACGACGCGTACCGCTCGAGCCTGTCGTGCATCCTGGTCGACAACATCGAGCGGCTGCTGGACTACGGACCGATCGGGCCGCGCTACTCCAACCTGACGCTGCAGGCGCTGCTCGTTCTCCTGAAGAAGCAGCCGCCCAAGGGACGAAAACTGCTCATCTTGTGCACGAGCAGCCGAAGGCAAGTGTTGGAAGACATGGAAATCCTGTCTGCGTTCACCGGAGTGCTCCACGTGCCGAACCTGTCGCAGCCCGAGCACCTGCTGTCGGTGCTCGAGGAGAGCGAGGCCTTCTCCAAGCGCGACCTGCAGAAGATACAGAGCGACCTGCGAGGAGCCAAAATCTTCATCGGTATAAAGAAGCTGCTCGCTCTAATCGACATGGTGAAGCAGACCGACGAAGAATACAGAGTGTTCAAGTTCCTCACGAAGTTGCAGGAGGAGGGCTGCGGATTACACGCGCATACCACGTCGCCCACGACCGACCACACCGCGACCCTTTGTACGTCCAGAACTCACGGCGCCAAACTAACCGAGTGCGCACCTTGCACTTCAGACCTCCGCAGCTATGAAATAGACTACGATGACGTCAGCGAATGCACCGACTCTCCCTTAGACATCGGCTATCACAAAAAAGAAATGGCCGCCAAAAGGACCGAGATATCGCCCGCCAAAATGCCGCTGATGCGAATTCGAAGCGTCGAGATTGTATTTGAAGACGAAATGTCGACTTGCACCGACACCGGGCTCCTGGACCGCAGCGTGGAACTCGTCGTCTCGGACAGTAGCGATGATTCCACTAAATGCGCGGACGGTATCGAGCCTCTACCGCCCGAGAAAGATTGCGACTCTGTGACGCTCAACGCTGACGGCGACTTGTCGGAGGACGACTCATCATCGTCGCTGAACAAACACGAAATACCTGAAATCGTTATTCCACAGCCGACGGCTCCGGCACACGCGACCCTTCTAGTTCGAACAGAGAACGTAACGTCGCGGACGGATAATTTGGCGATGTCGCCAAGTTTCTCTGAAATGGCAGACTCCGGTCGATACACAGAGGAAGCTAGTTCATCCCATATAGACATCGTTGCGATGTCTTACGAAGACGCATTGCTTTCAAACTCCTTTGACGTTATCAATTTAGAGGAACTTCCGGCCGCGACTCGCTCTTACGATTCGGACTCAAGCTTGAAACCAGAGACTCCGAGATTATTGGAATACATTGACAGTAACAGTGACAGCTCAGACACGGAAACGTGTGACTTGGACAGTCGAACGCATCCTCTGACTCCTCTCAACTTCATCTTGTCCGATGACGACCACACGGTGTGCGACAGTTCCGATTTGCAGTACGACTCTTTTGTAGATATGGGGAAGTTTTAA

Protein

MSSMRMKAAKCPSDELAITNCALIHQDDFPSDIKHIEVSTGPSQHFVFSIRFYNGVDRGTVGFSAPQRKWATLSIGQTIDVKPFKPSSAECLCSVTLEADFMMKKTTSTDPYDSEQMARDFLIQFANQVFTVGQQLAFLFQEKKVLSLIVKNLEAVDVQALAAGANAVPRRVRMGRLLPDASVQFDKAENSSLNLIGKAKGKQPRQSIINPDWDFGKMGIGGLDNEFNAIFRRAFASRVFPPEVVEQLGCKHVKGILLYGPPGTGKTLMARQIGKMLNAREPKIVNGPQILDKYVGESEANIRRLFADAEEEEKRCGANSGLHIIIFDEIDAICKARGSVGGNTGVHDTVVNQLLSKIDGVDQLNNILVIGMTNRRDMIDEALLRPGRLEVQMEIGLPDEKGRVQILNIHTKRMREYKKIAEDVDSMELATLTKNFSGAELEGLVRAAQSTAMNRLIKASSKVEVDPEAMEKLMVERTDFLHALENDIKPAFGTSAEALEHFLARGIVNWGSPVSSIFEDGQLYIQQARATEASGLVSVLLEGPPNSGKTALAAQLAKLSDFPFVKVCSPEDMVGFTETAKCLQIRKYFDDAYRSSLSCILVDNIERLLDYGPIGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRQVLEDMEILSAFTGVLHVPNLSQPEHLLSVLEESEAFSKRDLQKIQSDLRGAKIFIGIKKLLALIDMVKQTDEEYRVFKFLTKLQEEGCGLHAHTTSPTTDHTATLCTSRTHGAKLTECAPCTSDLRSYEIDYDDVSECTDSPLDIGYHKKEMAAKRTEISPAKMPLMRIRSVEIVFEDEMSTCTDTGLLDRSVELVVSDSSDDSTKCADGIEPLPPEKDCDSVTLNADGDLSEDDSSSSLNKHEIPEIVIPQPTAPAHATLLVRTENVTSRTDNLAMSPSFSEMADSGRYTEEASSSHIDIVAMSYEDALLSNSFDVINLEELPAATRSYDSDSSLKPETPRLLEYIDSNSDSSDTETCDLDSRTHPLTPLNFILSDDDHTVCDSSDLQYDSFVDMGKF

Description

Required for vesicle-mediated transport. Catalyzes the fusion of transport vesicles within the Golgi cisternae. Is also required for transport from the endoplasmic reticulum to the Golgi stack. Seems to function as a fusion protein required for the delivery of cargo proteins to all compartments of the Golgi stack independent of vesicle origin (By similarity).

Catalytic Activity

ATP + H2O = ADP + H(+) + phosphate

Cofactor

Mg(2+)

Subunit

Homohexamer.

Similarity

Belongs to the AAA ATPase family.

Keywords

ATP-binding   Complete proteome   Cytoplasm   ER-Golgi transport   Hydrolase   Magnesium   Metal-binding   Nucleotide-binding   Protein transport   Reference proteome   Repeat   Transport  

Feature

chain  Vesicle-fusing ATPase 2

Uniprot

A0A212F4N3   A0A2A4JAG1   A0A1P8W8N0   Q9U4Y6   A0A2A4J9E7   A0A2H1VZQ1   + More
A0A194Q822   A0A194QWW7   A0A1B6G249   A0A1B6MSH2   A0A2J7QCI0   A0A0M8ZZU6   A0A1B6C6V7   A0A067RCN5   A0A088A494   V9IKG1   A0A2A3E1X9   A0A1B0CWA3   A0A1Y1KMW4   A0A232F624   A0A1L8DW68   A0A0C9QG91   A0A0K8TTK0   E2C1M2   A0A158NK70   A0A0C9RWV1   A0A195BAX8   A0A195FBP9   F4W8G6   A0A151X6W0   Q17KM8   A0A195CZY0   A0A182GIC0   Q6EJD3   A0A0L7QQB5   A0A1Q3G3L1   A0A195EHT9   A0A0A1XP20   A0A1J1ILN9   A0A0L0C0M2   A0A026WU40   A0A0K8U5G2   A0A034VKS2   E2B0I4   W8AZC2   U5EWA2   B4MA92   B4L596   A0A1I8QDC7   B4NPN2   A0A3B0J6J7   T1PF76   B3NWQ9   B4Q231   A0A1A9WAQ9   D6X0K4   B4JMJ4   A0A0M3QY10   B3MS56   A0A0M5JE01   A0A1W4V7U5   B5DKI8   B4H3A0   A0A1B0FLT1   A0A1W4V6F2   B3LVS7   A0A182W7Y6   B4PQJ8   A0A182YDX3   B3P3Z3   A0A0B4LH53   P54351   T1IP97   A0A336LSI7   E0VAZ5   A0A146LHD2   B4HF87   A0A164W5F2   A0A0A9WYG7  

EC Number

3.6.4.6

Pubmed

22118469    10580154    26354079    24845553    28004739    28648823    + More
26369729    20798317    21347285    21719571    17510324    26483478    25830018    26108605    24508170    25348373    24495485    17994087    25315136    17550304    18362917    19820115    18057021    15632085    25244985    10731132    12537568    12537572    12537573    12537574    16110336    17569856    17569867    26109357    26109356    7642522    7624376    20566863    26823975    25401762   

EMBL

AGBW02010334    OWR48697.1    NWSH01002266    PCG68746.1    KY548397    APZ89680.1    + More
AF118384    AAF18300.1    PCG68747.1    ODYU01005445    SOQ46310.1    KQ459324    KPJ01539.1    KQ461073    KPJ09799.1    GECZ01017003    GECZ01013252    JAS52766.1    JAS56517.1    GEBQ01001090    JAT38887.1    NEVH01016289    PNF26292.1    KQ435789    KOX74480.1    GEDC01030689    GEDC01028091    GEDC01023926    GEDC01023325    GEDC01013279    JAS06609.1    JAS09207.1    JAS13372.1    JAS13973.1    JAS24019.1    KK852788    KDR16531.1    JR049505    AEY60986.1    KZ288470    PBC25332.1    AJWK01032124    AJWK01032125    AJWK01032126    GEZM01078395    GEZM01078394    JAV62742.1    NNAY01000881    OXU26085.1    GFDF01003394    JAV10690.1    GBYB01013543    JAG83310.1    GDAI01000338    JAI17265.1    GL451937    EFN78198.1    ADTU01018723    GBYB01013545    JAG83312.1    KQ976537    KYM81375.1    KQ981693    KYN37637.1    GL887908    EGI69457.1    KQ982472    KYQ56092.1    CH477222    EAT47276.1    KQ977110    KYN05689.1    JXUM01065739    JXUM01065740    KQ562363    KXJ76058.1    AY314995    AAQ83118.1    KQ414786    KOC60817.1    GFDL01000653    JAV34392.1    KQ978881    KYN27818.1    GBXI01001600    JAD12692.1    CVRI01000054    CRK99990.1    JRES01001065    KNC25860.1    KK107105    EZA59590.1    GDHF01030491    JAI21823.1    GAKP01015041    JAC43911.1    GL444608    EFN60826.1    GAMC01012390    JAB94165.1    GANO01001513    JAB58358.1    CH940655    EDW66151.1    CH933811    EDW06355.1    CH964291    EDW86472.1    OUUW01000003    SPP77784.1    KA646815    AFP61444.1    CH954180    EDV47221.1    CM000162    EDX01552.1    KQ971371    EFA10100.1    CH916371    EDV91937.1    CP012526    ALC46809.1    CH902622    EDV34611.1    KPU75214.1    CP012528    ALC49856.1    CH379063    EDY72037.1    CH479205    EDW30793.1    CCAG010020037    CH902617    EDV43701.2    CM000160    EDW97294.1    CH954181    EDV49167.1    AE014297    AHN57317.1    AHN57318.1    U30502    BT023784    U28836    JH431251    UFQT01000155    SSX20982.1    DS235019    EEB10551.1    GDHC01012273    JAQ06356.1    CH480815    EDW42261.1    LRGB01001348    KZS12965.1    GBHO01030815    GBHO01002606    GBHO01002601    JAG12789.1    JAG40998.1    JAG41003.1   

Proteomes

UP000007151    UP000218220    UP000053268    UP000053240    UP000235965    UP000053105    + More
UP000027135    UP000005203    UP000242457    UP000092461    UP000215335    UP000008237    UP000005205    UP000078540    UP000078541    UP000007755    UP000075809    UP000008820    UP000078542    UP000069940    UP000249989    UP000053825    UP000078492    UP000183832    UP000037069    UP000053097    UP000000311    UP000008792    UP000009192    UP000095300    UP000007798    UP000268350    UP000095301    UP000008711    UP000002282    UP000091820    UP000007266    UP000001070    UP000092553    UP000007801    UP000192221    UP000001819    UP000008744    UP000092444    UP000075920    UP000076408    UP000000803    UP000009046    UP000001292    UP000076858   

PRIDE

P54351   

Pfam

PF02933   CDC48_2        + More
PF00004   AAA
PF02359   CDC48_N
PF17862   AAA_lid_3

Interpro

IPR004201   Cdc48_dom2        + More
IPR003959   ATPase_AAA_core       
IPR003338   CDC4_N-term_subdom       
IPR027417   P-loop_NTPase       
IPR003960   ATPase_AAA_CS       
IPR009010   Asp_de-COase-like_dom_sf       
IPR003593   AAA+_ATPase       
IPR041569   AAA_lid_3       
IPR039812   Vesicle-fus_ATPase       
IPR029067   CDC48_domain_2-like_sf       

SUPFAM

SSF54585   SSF54585        + More
SSF52540   SSF52540       
SSF50692   SSF50692       

ProteinModelPortal

A0A212F4N3   A0A2A4JAG1   A0A1P8W8N0   Q9U4Y6   A0A2A4J9E7   A0A2H1VZQ1   + More
A0A194Q822   A0A194QWW7   A0A1B6G249   A0A1B6MSH2   A0A2J7QCI0   A0A0M8ZZU6   A0A1B6C6V7   A0A067RCN5   A0A088A494   V9IKG1   A0A2A3E1X9   A0A1B0CWA3   A0A1Y1KMW4   A0A232F624   A0A1L8DW68   A0A0C9QG91   A0A0K8TTK0   E2C1M2   A0A158NK70   A0A0C9RWV1   A0A195BAX8   A0A195FBP9   F4W8G6   A0A151X6W0   Q17KM8   A0A195CZY0   A0A182GIC0   Q6EJD3   A0A0L7QQB5   A0A1Q3G3L1   A0A195EHT9   A0A0A1XP20   A0A1J1ILN9   A0A0L0C0M2   A0A026WU40   A0A0K8U5G2   A0A034VKS2   E2B0I4   W8AZC2   U5EWA2   B4MA92   B4L596   A0A1I8QDC7   B4NPN2   A0A3B0J6J7   T1PF76   B3NWQ9   B4Q231   A0A1A9WAQ9   D6X0K4   B4JMJ4   A0A0M3QY10   B3MS56   A0A0M5JE01   A0A1W4V7U5   B5DKI8   B4H3A0   A0A1B0FLT1   A0A1W4V6F2   B3LVS7   A0A182W7Y6   B4PQJ8   A0A182YDX3   B3P3Z3   A0A0B4LH53   P54351   T1IP97   A0A336LSI7   E0VAZ5   A0A146LHD2   B4HF87   A0A164W5F2   A0A0A9WYG7  

PDB

3J99     E-value=0,     Score=2406

KEGG

692733    K06027 vesicle-fusing ATPase [EC:3.6.4.6] | (RefSeq) vesicle-fusing ATPase 1

GO

GO:0035494   GO:0005524   GO:0016887   GO:0070050   GO:0008021   GO:0007274   GO:0090160   GO:0048172   GO:0043195   GO:0005829   GO:1900073   GO:0007030   GO:0006914   GO:0098527   GO:0031629   GO:0005795   GO:0043001   GO:0048211   GO:0008582   GO:0060439   GO:0007269   GO:0016082   GO:0046872   GO:0007424   GO:0016192   GO:0005737   GO:0098793   GO:0035002   GO:0007430   GO:0015031   GO:0008270   GO:0003700   GO:0043565   GO:0007155   GO:0005515   GO:0008237   GO:0008152   GO:0016491   GO:0015930  

PANTHER

PTHR23078  

Subcellular location

Cytoplasm  

Length:

1049

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.0538700000000001

Exp number, first 60 AAs:

0.00015

Total prob of N-in:

0.00282

outside

1  -  1049

 

 

Pi

213.975098

Theta

150.621873

Tajima's D

1.81116

CLR

0.283492

CSRT

0.853557322133893

Interpretation

Uncertain