Gene

KWMTBOMO12764

Pre Gene Modal

BGIBMGA007158

Annotation

PREDICTED:_phosphatidylinositol_3-kinase_catalytic_subunit_type_3_[Bombyx_mori]

Location

Bomo_Chr21(-):13990219-14022473      View in EpiBrowser

Full name

Phosphatidylinositol 3-kinase catalytic subunit type 3      

Alternative Name

Phosphoinositide-3-kinase class 3

Location in the cell

Cytoplasmic   Reliability : 1.217 Mitochondrial   Reliability : 1.399 Nuclear   Reliability : 1.113

CDS

ATGAACGAAATAGACGACAAGTTTTATTATGTTTACAGTTCATCTTTGGATTACAAGGTCGAAATAAAAATTGGTACCCTTGAAGGCAAGAGAACTAAACCGGAATATGACAAACTACTATCAGATCCGATGTTGAAATTCTCAGGACTTTACCAAGATGGATGCTCAGACTTGTACGTCACCTGCCAAATCCTGAGTGATGGAGAACCTCTGGCGCTACCCGTCATGACCACTTATAAGGCATTCACTACTCGATGGAATTGGAATGAATGGGTAACCCTTCCGGTGTACTACAGTGATTTGCCCCGTAATGCTATACTAGCTATGACTATCTACGACTGTGCCGGTCCCGGTAAAGTGATGGTGGTCGGTGGCACGACCATATCGATATTTGGGAAGCACGGCATGCTGAGACAGGGGATGTTCGATCTGCGAGTGTGGCCCGGGGTTGAGGGCAGCGAGAAGACTCCGGGGAAGGCTCCGGACCGCGGCAAGGAGCAGATGCAGCGACTTGCGAAGCTCGCCAAGAAACACAGAAACGGACATTTGCCCAAGGTAGACTGGCTGGACAGGCTGACGTTTCGTGAAATCGAGATGATAAATGAAAAGGAGAAGCGAAGTTCGAATTATATGTATCTGATGGTGGAATTCCCGAGTGTTCAGATCGACGGCATCAATCACGCAGTTGTGTACTACGAACAGGACGGCGATGAGATGTACCAGTTCAGAGCTCAGGCCGAGCTGGTCACCGTACCGGATTGCGAAATATTGAAGGAGAACATAGTGGAGAGCAAGCAGCACCGGCTGGCGCGGTCGCTGCGCGCGGGGCAGTCGGCGCGCGACGCCAAGCCCTCCGCGCTCGAGCGGGACCAGCTCGCCGCGCTGCTGGCCGCGCCGCCCGCCCGCGCCGCGCCCGCCCTGCACCAGGACCTGCTGTGGAAGTTCCGGTTTTACTTGTCGTCGCACCGCCGCGCCCTCACCAAGTTCCTGGAATGCGTCAATTGGAATAGACCTGGCGAAGTGCGTCAGGCCCTCGCCATGATGAGGCAGTGGGCGCCCATGGACGTGGAAGATGCGTTAGAGCTGCTGACTCCGAAGTTCACGCATCCCGCTGTTAGGAAATACGCGATATCGAGATTGAGACAGGCTCCCGACGAGGATCTGCTGCTGTACCTGCTGCAACTGGTCCAGGCCCTGAAGTACGAGAGCTACTCGGAAATACACGAGGAGTACGCGCGCACGTGCGGCAAGGACATGCCGCTACCCGTGGAGCTGGACCCTCGCCTGGGGTACAGCCACCGAGACACTCTGCTGGCCACCTCGGCTCTGAGCGACGTGTCCGCGACCAGCCGGGACCCGGACGGCGCGGGGGGACAGGGGGGACCGGGGGGACCCACCGGGGGACAGACCCCGGGGACCGCCGCGCCCAGCGATGACGTCACGTCTGGTGGCGAGGAGCAGGGCAGCCTGGCGTGGTTCCTGATCGACCGCGCGTGCCGCAACCCGCAGCTGGCCAACTACCTGTACTGGTACCTGCTGGTGGAGTGCGAGGACGCGCCCGAGCCGCCGCCCCCGCGACACATGTACCTCGCCGTCATGCGCACCTTCAGCAGACGACTCGCCGACGGTAGCAGCTCGCACCAGCGCACGCGCTCGTTCCTGGCGCGGCAGCAGGTGTTCATCGACAAGCTGGTGCGGCTGGTGCGCTCCGTGGCGCGGGAGAGCGGCAGCCGCGCGCGCAAGGCCCAGCGCCTGCAGCAGCTGCTCGCAGACCCCGACGCCAACAAGTTCAACTTCGCCAGCTTCGAGCCCATGCCCTTCCCGCTCGACCCCAGCGTCACCATCAAAGGGATCGTCGCCAAGAAGGCCAGCCTCTTCAAGTCCGCCCTCATGCCCACCAAGCTCACCTTCCTCACGACCGAGGGCGTCGAGTACGATGCCATCTTCAAGTACGGCGACGACCTGCGCCAAGACCAGCTGATCCTGCAGATGATCACGCTGATGGACAAGCTGCTGAGGCGCGAGAACCTGGACCTGAAGCTGACGCCGTACCGCGTGCTGGCCACCAGCTCCAAGCACGGCTTCCTGCAGTTCGTGGAGTCCGTGACGGTGGCGGAGGCGCTGGCCACGGAGGGCAGCATACAGAACTTCTTCAGGAAGCACAACCCGTGCGAGGGCGCGCCCTACGGCATCAAGCCCGAGACCATGGACACGTACATCCGCAGCTGCGCCGGATACTGCGTCATCACCTATCTGCTCGGTGTGGGTGACCGGCATCTGGACAACCTGCTGCTGAGCCGCAGCGGCGCGCTGTTCCACATCGACTTCGGCTACATCCTGGGTCGGGATCCGAAGCCGCTTCCGCCACCCATGAAGCTCAGCAAGGAGATGGTGGAGGCCATGGGCGGGGTCAACTCGGACCACTACCACGCCTTCCGCAAGCTCACCTACACCGCCTTCCTGCATCTCAGACGTCATGCAAACCTGATGCTGAACCTGTTCTCGCTGATGGTGGACGCCTCCGTCCCGGACATTGCGCTGGAGCCGGACAAGGCCGTCAAGAAAGTGCAGGATAAGTTCAGGCTGGATCTCGGCGACGAGGAAGCCATTCACTACCTGCAGAATCTCTTGGACATGTCAGTGACAGCTGTCATGGCGGTCCTCGTCGAACAATTCCACAAGTTCGCTCAATACTGGAGGAAATGA

Protein

MNEIDDKFYYVYSSSLDYKVEIKIGTLEGKRTKPEYDKLLSDPMLKFSGLYQDGCSDLYVTCQILSDGEPLALPVMTTYKAFTTRWNWNEWVTLPVYYSDLPRNAILAMTIYDCAGPGKVMVVGGTTISIFGKHGMLRQGMFDLRVWPGVEGSEKTPGKAPDRGKEQMQRLAKLAKKHRNGHLPKVDWLDRLTFREIEMINEKEKRSSNYMYLMVEFPSVQIDGINHAVVYYEQDGDEMYQFRAQAELVTVPDCEILKENIVESKQHRLARSLRAGQSARDAKPSALERDQLAALLAAPPARAAPALHQDLLWKFRFYLSSHRRALTKFLECVNWNRPGEVRQALAMMRQWAPMDVEDALELLTPKFTHPAVRKYAISRLRQAPDEDLLLYLLQLVQALKYESYSEIHEEYARTCGKDMPLPVELDPRLGYSHRDTLLATSALSDVSATSRDPDGAGGQGGPGGPTGGQTPGTAAPSDDVTSGGEEQGSLAWFLIDRACRNPQLANYLYWYLLVECEDAPEPPPPRHMYLAVMRTFSRRLADGSSSHQRTRSFLARQQVFIDKLVRLVRSVARESGSRARKAQRLQQLLADPDANKFNFASFEPMPFPLDPSVTIKGIVAKKASLFKSALMPTKLTFLTTEGVEYDAIFKYGDDLRQDQLILQMITLMDKLLRRENLDLKLTPYRVLATSSKHGFLQFVESVTVAEALATEGSIQNFFRKHNPCEGAPYGIKPETMDTYIRSCAGYCVITYLLGVGDRHLDNLLLSRSGALFHIDFGYILGRDPKPLPPPMKLSKEMVEAMGGVNSDHYHAFRKLTYTAFLHLRRHANLMLNLFSLMVDASVPDIALEPDKAVKKVQDKFRLDLGDEEAIHYLQNLLDMSVTAVMAVLVEQFHKFAQYWRK

Description

Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).
Catalytic subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Involved in the transport of lysosomal enzyme precursors to lysosomes. Required for transport from early to late endosomes (By similarity).

Catalytic Activity

a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol) + ATP = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol 3-phosphate) + ADP + H(+)

Cofactor

Mn(2+)

Subunit

Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit pik3c3, the regulatory subunit pik3r4 and becn1 associating with additional regulatory/auxilliary subunits to form alternative complex forms.
Component of the PI3K (PI3KC3/PI3K-III/class III phosphatidylinositol 3-kinase) complex the core of which is composed of the catalytic subunit PIK3C3, the regulatory subunit PIK3R4 and BECN1 associating with additional regulatory/auxilliary subunits to form alternative complex forms. Alternative complex forms containing a forth regulatory subunit in a mutually exclusive manner are: the PI3K complex I (PI3KC3-C1) containing ATG14, and the PI3K complex II (PI3KC3-C2) containing UVRAG. PI3KC3-C1 displays a V-shaped architecture with PIK3R4 serving as a bridge between PIK3C3 and the ATG14:BECN1 subcomplex (By similarity). Both, PI3KC3-C1 and PI3KC3-C2, can associate with further regulatory subunits such as RUBCN, SH3GLB1/Bif-1 and AMBRA1 (PubMed:17589504). PI3KC3-C1 probably associates with PIK3CB (PubMed:21059846). Interacts with RAB7A in the presence of PIK3R4 (By similarity). Interacts with AMBRA1 (PubMed:17589504). Interacts with BECN1P1/BECN2 (By similarity). Interacts with SLAMF1 (PubMed:22493499). May be a component of a complex composed of RAB5A (in GDP-bound form), DYN2 and PIK3C3 (By similarity). Interacts with NCKAP1L (By similarity).

Similarity

Belongs to the PI3/PI4-kinase family.

Keywords

ATP-binding   Autophagy   Cell cycle   Cell division   Kinase   Manganese   Nucleotide-binding   Transferase   Alternative splicing   Complete proteome   Cytoplasmic vesicle   Endosome   Phosphoprotein   Reference proteome  

Feature

chain  Phosphatidylinositol 3-kinase catalytic subunit type 3
splice variant  In isoform 2.

Uniprot

A0A194QLY7   A0A212F9V4   H9JCB3   A0A2H1VUC4   A0A1Y1KF83   A0A2J7PWP0   + More
A0A067RKC6   A0A0P4VM95   T1HML4   A0A023F599   D6W9A3   A0A195BWH8   A0A154PFT4   E2B101   A0A195D9L4   A0A0L7RF14   A0A151WST9   A0A195CQ30   A0A195FQL7   A0A2R7VZ13   A0A3L8D828   A0A2A3EGH2   E0VQ65   E9IDA9   A0A2J7PWM9   A0A1S4EPX6   A0A2S2Q897   T1JEU2   J9K3U7   A0A310SPJ0   A0A2H8TFV5   A0A2P8XVN6   A0A1L8DMC6   B0W5Z6   C3Y1V6   A0A1Q3FDS2   A0A146P539   A0A3P8UT11   A0A3Q2FWL0   A0A2U9B189   A0A1L8I323   Q6AZN6   A0A3B4WVD8   A0A3B4TW17   G3Q5W9   A0A1A9WPA1   A0A3Q2PAN6   A0A3Q1FZN2   A0A3P8S0F3   A0A1W4ZW98   H2LUA0   A0A3P9LW04   A0A1B0CTU6   A0A1W4ZNA0   A0A1U8CKK8   A0A2I4CLW9   A0A3B3C9C2   A0A3Q2E6U0   A0A3Q3AKW4   G3Q5X0   Q6PF93   A0A3B5Q9A0   A0A1V4KTV3   I3K560   A0A3B3WQT0   A0A1U7RYK9   A0A3B3W1B4   A0A3S2PT87   A0A1J1ITM6   A0A147ASV9   A0A1A8RSW8   G1KPF8   F1Q9F3   A0A1A8NAL0   A0A1A8I9L5   A0A1A8UHN0   G1SX90   A0A3P9IUA7   A0A3Q3KSY3   A0A1A8C650   A0A146Y7U9   A0A3B4X232   A0A151MWA8   A0A3B1J562   A0A3Q2UEL4   A0A1S3SDV9   A0A3B4EA32   T1E4S4   A0A402EVR1   A0A1A8H9A0   A0A0F8B087   A0A060WR34   A0A3B3HSF3   A0A3P9KBE9   A0A1S3SDV8   J3SCI6   A0A3B4F817   A0A2I4CLX1   A0A3Q2VFU5   K9IP63  

EC Number

2.7.1.137

Pubmed

26354079    22118469    19121390    28004739    24845553    27129103    + More
25474469    18362917    19820115    20798317    30249741    20566863    21282665    29403074    18563158    24487278    27762356    17554307    29451363    15489334    17589504    21183079    21059846    22493499    23332761    24089209    23542700    25186727    23594743    21993624    22293439    25329095    15592404    23758969    25835551    24755649    23025625   

EMBL

KQ458981    KPJ04466.1    AGBW02009556    OWR50514.1    BABH01031593    BABH01031594    + More
ODYU01004213    SOQ43844.1    GEZM01087202    JAV58850.1    NEVH01020871    PNF20745.1    KK852460    KDR23478.1    GDKW01000953    JAI55642.1    ACPB03020499    GBBI01002434    JAC16278.1    KQ971312    EEZ98191.1    KQ976401    KYM92635.1    KQ434886    KZC10178.1    GL444767    EFN60627.1    KQ981082    KYN09610.1    KQ414606    KOC69577.1    KQ982769    KYQ50916.1    KQ977444    KYN02740.1    KQ981335    KYN42592.1    KK854182    PTY12724.1    QOIP01000011    RLU16647.1    KZ288267    PBC30101.1    DS235389    EEB15521.1    GL762454    EFZ21498.1    PNF20743.1    GGMS01004752    MBY73955.1    JH432130    ABLF02026968    KQ762031    OAD56362.1    GFXV01001144    MBW12949.1    PYGN01001278    PSN36071.1    GFDF01006570    JAV07514.1    DS231845    EDS35983.1    GG666480    EEN65846.1    GFDL01009334    JAV25711.1    GCES01147447    JAQ38875.1    CP026244    AWO97541.1    CM004466    OCU02735.1    BC077528    AJWK01027997    BC024675    BC057678    LSYS01001587    OPJ87851.1    AERX01017946    CM012454    RVE60403.1    CVRI01000057    CRL02454.1    GCES01004660    JAR81663.1    HAEH01003759    HAEI01009684    SBS08463.1    BX510349    HAEF01021582    HAEG01001868    SBR66118.1    HAED01007585    HAEE01003542    SBQ93797.1    HADY01004517    HAEJ01007133    SBS47590.1    AAGW02032208    AAGW02032209    AAGW02032210    HADZ01011436    HAEA01007987    SBP75377.1    GCES01036072    JAR50251.1    AKHW03004724    KYO28843.1    AC189108    AC192000    GAAZ01001775    JAA96168.1    BDOT01000049    GCF48210.1    HAEB01002391    HAEC01011695    SBQ79912.1    KQ042129    KKF18716.1    FR904686    CDQ69823.1    JU174894    GBEX01002474    AFJ50420.1    JAI12086.1    GABZ01004243    JAA49282.1   

Proteomes

UP000053268    UP000007151    UP000005204    UP000235965    UP000027135    UP000015103    + More
UP000007266    UP000078540    UP000076502    UP000000311    UP000078492    UP000053825    UP000075809    UP000078542    UP000078541    UP000279307    UP000242457    UP000009046    UP000079169    UP000007819    UP000245037    UP000002320    UP000001554    UP000265000    UP000265120    UP000265020    UP000246464    UP000186698    UP000261360    UP000261420    UP000007635    UP000091820    UP000257200    UP000265080    UP000192224    UP000001038    UP000265180    UP000092461    UP000189706    UP000192220    UP000261560    UP000264800    UP000000589    UP000002852    UP000190648    UP000005207    UP000261480    UP000189705    UP000261500    UP000183832    UP000001646    UP000000437    UP000001811    UP000265200    UP000261660    UP000050525    UP000018467    UP000000539    UP000087266    UP000261440    UP000288954    UP000193380    UP000261460    UP000264840   

PRIDE

H9JCB3    Q6AZN6    Q6PF93   

Pfam

PF00454   PI3_PI4_kinase        + More
PF00613   PI3Ka
PF00792   PI3K_C2
PF00476   DNA_pol_A
PF04991   LicD
PF00226   DnaJ
PF09320   DUF1977
PF12578   3-PAP
PF06602   Myotub-related

Interpro

IPR008290   PI3K_Vps34        + More
IPR036940   PI3/4_kinase_cat_sf       
IPR015433   PI_Kinase       
IPR016024   ARM-type_fold       
IPR035892   C2_domain_sf       
IPR001263   PI3K_accessory_dom       
IPR042236   PI3K_accessory_sf       
IPR002420   PI3K_C2_dom       
IPR011009   Kinase-like_dom_sf       
IPR000403   PI3/4_kinase_cat_dom       
IPR018936   PI3/4_kinase_CS       
IPR001098   DNA-dir_DNA_pol_A_palm_dom       
IPR002298   DNA_polymerase_A       
IPR036322   WD40_repeat_dom_sf       
IPR007074   LicD_fam       
IPR015399   DUF1977_DnaJ-like       
IPR022587   MTMR12-like_C       
IPR029021   Prot-tyrosine_phosphatase-like       
IPR036869   J_dom_sf       
IPR010569   Myotubularin-like_Pase_dom       
IPR001623   DnaJ_domain       

SUPFAM

SSF48371   SSF48371        + More
SSF56112   SSF56112       
SSF50978   SSF50978       
SSF46565   SSF46565       
SSF52799   SSF52799       

Gene 3D

1.10.1070.11     1.25.40.70     2.60.40.150     1.10.287.110    

CDD

cd06257  DnaJ

ProteinModelPortal

A0A194QLY7   A0A212F9V4   H9JCB3   A0A2H1VUC4   A0A1Y1KF83   A0A2J7PWP0   + More
A0A067RKC6   A0A0P4VM95   T1HML4   A0A023F599   D6W9A3   A0A195BWH8   A0A154PFT4   E2B101   A0A195D9L4   A0A0L7RF14   A0A151WST9   A0A195CQ30   A0A195FQL7   A0A2R7VZ13   A0A3L8D828   A0A2A3EGH2   E0VQ65   E9IDA9   A0A2J7PWM9   A0A1S4EPX6   A0A2S2Q897   T1JEU2   J9K3U7   A0A310SPJ0   A0A2H8TFV5   A0A2P8XVN6   A0A1L8DMC6   B0W5Z6   C3Y1V6   A0A1Q3FDS2   A0A146P539   A0A3P8UT11   A0A3Q2FWL0   A0A2U9B189   A0A1L8I323   Q6AZN6   A0A3B4WVD8   A0A3B4TW17   G3Q5W9   A0A1A9WPA1   A0A3Q2PAN6   A0A3Q1FZN2   A0A3P8S0F3   A0A1W4ZW98   H2LUA0   A0A3P9LW04   A0A1B0CTU6   A0A1W4ZNA0   A0A1U8CKK8   A0A2I4CLW9   A0A3B3C9C2   A0A3Q2E6U0   A0A3Q3AKW4   G3Q5X0   Q6PF93   A0A3B5Q9A0   A0A1V4KTV3   I3K560   A0A3B3WQT0   A0A1U7RYK9   A0A3B3W1B4   A0A3S2PT87   A0A1J1ITM6   A0A147ASV9   A0A1A8RSW8   G1KPF8   F1Q9F3   A0A1A8NAL0   A0A1A8I9L5   A0A1A8UHN0   G1SX90   A0A3P9IUA7   A0A3Q3KSY3   A0A1A8C650   A0A146Y7U9   A0A3B4X232   A0A151MWA8   A0A3B1J562   A0A3Q2UEL4   A0A1S3SDV9   A0A3B4EA32   T1E4S4   A0A402EVR1   A0A1A8H9A0   A0A0F8B087   A0A060WR34   A0A3B3HSF3   A0A3P9KBE9   A0A1S3SDV8   J3SCI6   A0A3B4F817   A0A2I4CLX1   A0A3Q2VFU5   K9IP63  

PDB

3LS8     E-value=0,     Score=1706

KEGG

101743450    K00914 phosphatidylinositol 3-kinase [EC:2.7.1.137] | (RefSeq) phosphatidylinositol 3-kinase catalyt

PATHWAY

00562    Inositol phosphate metabolism - Bombyx mori (domestic silkworm)
01100    Metabolic pathways - Bombyx mori (domestic silkworm)
04070    Phosphatidylinositol signaling system - Bombyx mori (domestic silkworm)
04136    Autophagy - other - Bombyx mori (domestic silkworm)
04140    Autophagy - animal - Bombyx mori (domestic silkworm)
04145    Phagosome - Bombyx mori (domestic silkworm)

GO

GO:0005524   GO:0016303   GO:0048015   GO:0036092   GO:0005737   GO:0006468   GO:0000407   GO:0006897   GO:0034272   GO:0000045   GO:0005768   GO:0005777   GO:0034271   GO:0030242   GO:0046854   GO:0016020   GO:0006261   GO:0003677   GO:0003887   GO:0016301   GO:0030496   GO:0032465   GO:0007049   GO:0051301   GO:0006914   GO:0016021   GO:0045335   GO:0004672   GO:0016236   GO:0016485   GO:0043201   GO:0045022   GO:0005829   GO:0035004   GO:0042149   GO:0035032   GO:0009267   GO:0044754   GO:0034497   GO:0050708   GO:0006497   GO:0005770   GO:0007032   GO:0005515   GO:0005488   GO:0004003   GO:0005634   GO:0006289   GO:0016286  

PANTHER

PTHR10048  

Subcellular location

Midbody  
Late endosome   As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (PubMed:24089209).   With evidence from 3 publications.
Cytoplasmic vesicle   As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (PubMed:24089209).   With evidence from 3 publications.
Autophagosome   As component of the PI3K complex I localized to pre-autophagosome structures. As component of the PI3K complex II localized predominantly to endosomes (By similarity). Localizes also to discrete punctae along the ciliary axoneme and to the base of the ciliary axoneme (PubMed:24089209).   With evidence from 3 publications.

Length:

901

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

1.15403

Exp number, first 60 AAs:

5e-05

Total prob of N-in:

0.03043

outside

1  -  901

 

 

Pi

22.718261

Theta

2.259455

Tajima's D

0.230657

CLR

0.938198

CSRT

0.480525973701315

Interpretation

Uncertain