Gene

KWMTBOMO12739  Validated by peptides from experiments

Pre Gene Modal

BGIBMGA007171

Annotation

PREDICTED:_probable_tRNA_(guanine(26)-N(2))-dimethyltransferase_isoform_X1_[Bombyx_mori]

Location

Bomo_Chr21(-):13575893-13582429      View in EpiBrowser

Full name

tRNA (guanine(26)-N(2))-dimethyltransferase       + More
Probable tRNA (guanine(26)-N(2))-dimethyltransferase      

Alternative Name

tRNA 2,2-dimethylguanosine-26 methyltransferase
tRNA(guanine-26,N(2)-N(2)) methyltransferase
tRNA(m(2,2)G26)dimethyltransferase

Location in the cell

Nuclear   Reliability : 3.221

CDS

ATGAGAATGGAAACTATATCAAACTTAAAAACTATAAAAGAAGGCCAAGCAGAGATATGTTTAACTACAGAGAAGGTTTTTTACAATCCTGTTCAAGAATTCAACCGTGATCTTAGTATAGCTGTGCTTACGCTGTTTATTGAAGATTATAAAGCGGAAAAGTTGGCACGTTTCGAAAAAAAACAAAAAAAACTGGAAACCGTGCAAGATGAAGAAAGCGGAGGTAATCCAGAGCCAAAAATAACAATCTTAGAAGCACTATCAGCAACAGGCCTCAGAAGCATCCGTTATGCTAAAGAAATACCATATGCTACCAATATCATTGCAAATGATCTCTCAGAACAAGCTGTTGAGACAATTAAGCATAATATTGAACATAACCAGGTGTCACGCATCATAGAAACTAGTCATGATGATGCTTGTATGTTAATGTACAAACATAAACACCCTTCAAAGCGTTTTGCGGCTATTGATCTGGACCCTTATGGATGCCCTTCAATATTCCTCGACTCTGCAGTACAGAGCATTCAAGATGGTGGTTTGCTACTCGTTACAGCAACTGATATGGCAGTTTTAGCCGGCAACTCACCAGAGACATGTTATTGTAAATATGGTGCTGTTAGCTTAAAGACCAAATGCTGTCATGAGATGGCCCTCAGGATAATGCTACAATGTATAGAACAGCATGCAAATCGTTACAGCAGATACATTGTTCCAATTCTTAGTATATCAGCCGATTTCTACATACGTGTTTTTGTCAAAATATACTCTGGTGCCGTGCACTGCAAGAAGACAACAAGCAAGTTGTCAATGGTTTACCACTGCACAGGCTGCGATAACTTGACCCTGCAACCTCTGGGTGGCTTAAAACCGAACCCGTCTGAGAAAAACCCAAACCAAATGAAGGCTTATCTTCCTGCAGCACCACCTATAGGCGAATACTGCACCAACTGCAACCAGAGGCATCATCTAGGCGGTCCAATATGGTCGGCTCCGATACACGACGAATCCTTCGTGTCACGCGTCCTAACGTACGTAGAAGAGAATTCTCAACTCTTCGGAACGGCGAAACGCATTGAAGGCGTCTTGTCTATGGTCCGAGAGGAGTTGAACGACGTCCCTCTGTACTACACCATAGACAAGTTATTCGGTAGAGTGCACTTGCAGACAATGCCGATGTTGATAATGAGATCAGCAATTATTAACGCTGGCTATAAAGTATCTTATTCGCACGCATCGAAAATGTCAGTGAAGACGAACGCTCCACCACAATTGATGTGGGACATCATCAGGACGTGGGAGAAAACACACCCAATCAAGCAGAGCAAACTGGTTTCAGATCCTGTAACGAAGCACATACTATCTCAGGATATAAAGAATAATGTCGATTTGTCGGTGAATGAGCTAGCCAATCCGCTGAGCCGCCGTGACGGCAGGCTTCGCTTCCAGATCAATCCTGCACCGTTCTGGGGACCTGGGTCCAGAGCCGCAGTCAATATCGGCGACGACAAAATGTCGAAGTCTGTTAAAAATCAGAACAAGAACAGGAAGAAAGAAAACAATGTTAAAAGAAAACATTCGGATACAAGTGACGATGATTCACGGAAAAAAGTTGATATGGAATTAAAACATAATGAATAA

Protein

MRMETISNLKTIKEGQAEICLTTEKVFYNPVQEFNRDLSIAVLTLFIEDYKAEKLARFEKKQKKLETVQDEESGGNPEPKITILEALSATGLRSIRYAKEIPYATNIIANDLSEQAVETIKHNIEHNQVSRIIETSHDDACMLMYKHKHPSKRFAAIDLDPYGCPSIFLDSAVQSIQDGGLLLVTATDMAVLAGNSPETCYCKYGAVSLKTKCCHEMALRIMLQCIEQHANRYSRYIVPILSISADFYIRVFVKIYSGAVHCKKTTSKLSMVYHCTGCDNLTLQPLGGLKPNPSEKNPNQMKAYLPAAPPIGEYCTNCNQRHHLGGPIWSAPIHDESFVSRVLTYVEENSQLFGTAKRIEGVLSMVREELNDVPLYYTIDKLFGRVHLQTMPMLIMRSAIINAGYKVSYSHASKMSVKTNAPPQLMWDIIRTWEKTHPIKQSKLVSDPVTKHILSQDIKNNVDLSVNELANPLSRRDGRLRFQINPAPFWGPGSRAAVNIGDDKMSKSVKNQNKNRKKENNVKRKHSDTSDDDSRKKVDMELKHNE

Description

Dimethylates a single guanine residue at position 26 of most tRNAs using S-adenosyl-L-methionine as donor of the methyl groups.

Catalytic Activity

guanosine(26) in tRNA + 2 S-adenosyl-L-methionine = 2 H(+) + N(2)-dimethylguanosine(26) in tRNA + 2 S-adenosyl-L-homocysteine

Similarity

Belongs to the class I-like SAM-binding methyltransferase superfamily. Trm1 family.

Keywords

Complete proteome   Methyltransferase   Reference proteome   RNA-binding   S-adenosyl-L-methionine   Transferase   tRNA processing   tRNA-binding  

Feature

chain  Probable tRNA (guanine(26)-N(2))-dimethyltransferase

Uniprot

H9JCC6   A0A2W1BJJ1   S4NU39   A0A2A4JVD7   A0A2H1V4U5   A0A212FCN3   + More
A0A3S2LAJ8   A0A0N1PHA6   A0A2J7QEC1   A0A2J7QEA8   A0A182P4Q4   A0A182K0H0   A0A182V8A6   A0A1S4H1Y3   A0A182G3N0   A0A182XN82   A0A2C9GR22   A0A182LZX0   A0A182TK31   Q16T94   A0A1Q3EXP2   A0A182QVX7   B4P214   B0WIP5   A0A182WGI4   A0A182NBQ8   A0A1Q3EY09   Q9VK89   Q7PM72   B4IE89   B4Q3T1   A0A182Y0N0   A0A1W4VG93   A0A182UGM8   A0A1I8MVG1   A0A2M4AH45   A0A182R3T0   A0A0L0C5T6   A0A0T6B1C2   W5JES7   A0A2M4AGU6   N6TS59   U4U7Z1   B3N3U7   A0A2M4AH49   A0A1W4XGL0   A0A2M3Z7J1   A0A2M3Z7A9   A0A182F0X8   A0A2M4BID4   A0A2M4BIF2   A0A2M4BID2   A0A2M4BJ34   B4G8T1   A0A1I8NLQ9   Q29MS6   A0A1A9ZK67   A0A1B6CIS3   A0A3B0JE73   A0A084WU36   A0A182JDC6   A0A1Y1KYG8   B3MNU2   A0A1A9UH82   B4MVG1   B4JCQ3   A0A1A9WZQ4   D6WW07   B4KJ40   A0A182T004   A0A1B0AP44   A0A1A9XW12   E2BTE9   W8B9H9   A0A195FY65   F4WLB2   A0A195CRX9   E9J721   A0A158NXB7   A0A0C9QSM6   A0A151WRC9   A0A1B0FAT7   A0A1L8DRG6   A0A034WK11   A0A0J7KUH1   A0A0M3QU48   A0A0K8U6Q6   A0A1B0CYT1   A0A2A3EI55   A0A088AGI4   A0A336K6H1   A0A0A1X631   A0A151IT40   A0A0L7QVK7   A0A195BPE7   A0A1B6IAC8   A0A1B6IPS7   A0A232EPC0   E2AB22   A0A146MC45  

EC Number

2.1.1.216

Pubmed

19121390    28756777    23622113    22118469    26354079    12364791    + More
26483478    17510324    17994087    17550304    10731132    12537572    12537569    22936249    25244985    25315136    26108605    20920257    23761445    23537049    15632085    24438588    28004739    18362917    19820115    20798317    24495485    21719571    21282665    21347285    25348373    25830018    28648823    26823975   

EMBL

BABH01031550    KZ150005    PZC75242.1    GAIX01011961    JAA80599.1    NWSH01000508    + More
PCG75971.1    ODYU01000681    SOQ35870.1    AGBW02009157    OWR51499.1    RSAL01000317    RVE42592.1    KQ460882    KPJ11489.1    NEVH01015318    PNF26917.1    PNF26916.1    AAAB01008980    JXUM01141868    KQ569321    KXJ68666.1    APCN01001775    AXCM01001134    CH477657    EAT37696.1    GFDL01014966    JAV20079.1    AXCN02000684    CM000157    EDW88185.1    DS231951    EDS28622.1    GFDL01014879    JAV20166.1    AE014134    AY051836    EAA14208.5    CH480831    EDW45916.1    CM000361    CM002910    EDX04806.1    KMY89918.1    GGFK01006794    MBW40115.1    JRES01000869    KNC27650.1    LJIG01016428    KRT80631.1    ADMH02001427    ETN62576.1    GGFK01006685    MBW40006.1    APGK01056813    KB741277    ENN71141.1    KB631786    ERL86065.1    CH954177    EDV58799.1    GGFK01006795    MBW40116.1    GGFM01003742    MBW24493.1    GGFM01003662    MBW24413.1    GGFJ01003678    MBW52819.1    GGFJ01003676    MBW52817.1    GGFJ01003675    MBW52816.1    GGFJ01003677    MBW52818.1    CH479180    EDW28761.1    CH379060    EAL33617.1    GEDC01023934    JAS13364.1    OUUW01000004    SPP78953.1    ATLV01026995    KE525421    KFB53730.1    GEZM01073277    JAV65210.1    CH902620    EDV32129.1    CH963857    EDW76506.1    CH916368    EDW04217.1    KQ971361    EFA08644.2    CH933807    EDW11402.1    JXJN01001139    GL450402    EFN81030.1    GAMC01008635    JAB97920.1    KQ981169    KYN45376.1    GL888207    EGI65075.1    KQ977349    KYN03395.1    GL768421    EFZ11336.1    ADTU01029642    GBYB01006739    JAG76506.1    KQ982807    KYQ50363.1    CCAG010023607    GFDF01005006    JAV09078.1    GAKP01004285    JAC54667.1    LBMM01003110    KMQ93951.1    CP012523    ALC40043.1    GDHF01029972    JAI22342.1    AJVK01009332    KZ288235    PBC31397.1    UFQS01000093    UFQT01000093    SSW99228.1    SSX19608.1    GBXI01008154    JAD06138.1    KQ981047    KYN10034.1    KQ414726    KOC62650.1    KQ976433    KYM87327.1    GECU01023810    JAS83896.1    GECU01018787    GECU01005734    JAS88919.1    JAT01973.1    NNAY01002952    OXU20229.1    GL438234    EFN69298.1    GDHC01010912    GDHC01001281    JAQ07717.1    JAQ17348.1   

Proteomes

UP000005204    UP000218220    UP000007151    UP000283053    UP000053240    UP000235965    + More
UP000075885    UP000075881    UP000075903    UP000069940    UP000249989    UP000076407    UP000075840    UP000075883    UP000075902    UP000008820    UP000075886    UP000002282    UP000002320    UP000075920    UP000075884    UP000000803    UP000007062    UP000001292    UP000000304    UP000076408    UP000192221    UP000095301    UP000075900    UP000037069    UP000000673    UP000019118    UP000030742    UP000008711    UP000192223    UP000069272    UP000008744    UP000095300    UP000001819    UP000092445    UP000268350    UP000030765    UP000075880    UP000007801    UP000078200    UP000007798    UP000001070    UP000091820    UP000007266    UP000009192    UP000075901    UP000092460    UP000092443    UP000008237    UP000078541    UP000007755    UP000078542    UP000005205    UP000075809    UP000092444    UP000036403    UP000092553    UP000092462    UP000242457    UP000005203    UP000078492    UP000053825    UP000078540    UP000215335    UP000000311   

PRIDE

H9JCC6    Q9VK89   

Pfam

PF02005   TRM        + More
PF15065   NCU-G1
PF08920   SF3b1
PF12494   DUF3695
PF00248   Aldo_ket_red

Interpro

IPR029063   SAM-dependent_MTases        + More
IPR002905   Trm1       
IPR042296   tRNA_met_Trm1_C       
IPR029382   NCU-G1       
IPR016024   ARM-type_fold       
IPR034085   TOG       
IPR038737   SF3b_su1-like       
IPR022179   DUF3695       
IPR011989   ARM-like       
IPR015016   SF3b_su1       
IPR036812   NADP_OxRdtase_dom_sf       
IPR018170   Aldo/ket_reductase_CS       
IPR020471   Aldo/keto_reductase       
IPR023210   NADP_OxRdtase_dom       

SUPFAM

SSF53335   SSF53335        + More
SSF48371   SSF48371       
SSF51430   SSF51430       

Gene 3D

3.30.56.70     1.25.10.10     3.20.20.100    

CDD

cd06660  Aldo_ket_red

ProteinModelPortal

H9JCC6   A0A2W1BJJ1   S4NU39   A0A2A4JVD7   A0A2H1V4U5   A0A212FCN3   + More
A0A3S2LAJ8   A0A0N1PHA6   A0A2J7QEC1   A0A2J7QEA8   A0A182P4Q4   A0A182K0H0   A0A182V8A6   A0A1S4H1Y3   A0A182G3N0   A0A182XN82   A0A2C9GR22   A0A182LZX0   A0A182TK31   Q16T94   A0A1Q3EXP2   A0A182QVX7   B4P214   B0WIP5   A0A182WGI4   A0A182NBQ8   A0A1Q3EY09   Q9VK89   Q7PM72   B4IE89   B4Q3T1   A0A182Y0N0   A0A1W4VG93   A0A182UGM8   A0A1I8MVG1   A0A2M4AH45   A0A182R3T0   A0A0L0C5T6   A0A0T6B1C2   W5JES7   A0A2M4AGU6   N6TS59   U4U7Z1   B3N3U7   A0A2M4AH49   A0A1W4XGL0   A0A2M3Z7J1   A0A2M3Z7A9   A0A182F0X8   A0A2M4BID4   A0A2M4BIF2   A0A2M4BID2   A0A2M4BJ34   B4G8T1   A0A1I8NLQ9   Q29MS6   A0A1A9ZK67   A0A1B6CIS3   A0A3B0JE73   A0A084WU36   A0A182JDC6   A0A1Y1KYG8   B3MNU2   A0A1A9UH82   B4MVG1   B4JCQ3   A0A1A9WZQ4   D6WW07   B4KJ40   A0A182T004   A0A1B0AP44   A0A1A9XW12   E2BTE9   W8B9H9   A0A195FY65   F4WLB2   A0A195CRX9   E9J721   A0A158NXB7   A0A0C9QSM6   A0A151WRC9   A0A1B0FAT7   A0A1L8DRG6   A0A034WK11   A0A0J7KUH1   A0A0M3QU48   A0A0K8U6Q6   A0A1B0CYT1   A0A2A3EI55   A0A088AGI4   A0A336K6H1   A0A0A1X631   A0A151IT40   A0A0L7QVK7   A0A195BPE7   A0A1B6IAC8   A0A1B6IPS7   A0A232EPC0   E2AB22   A0A146MC45  

PDB

2YTZ     E-value=7.47797e-47,     Score=473

KEGG

101738076    K00555 tRNA (guanine26-N2/guanine27-N2)-dimethyltransferase [EC:2.1.1.215 2.1.1.216] | (RefSeq) prob

GO

GO:0000049   GO:0004809   GO:0016021   GO:0002940   GO:0005634   GO:0003729   GO:0000245   GO:0016491   GO:0003723   GO:0008033   GO:0004386   GO:0005515   GO:0003677   GO:0004003   GO:0006289   GO:0016286  

PANTHER

PTHR10631   PTHR12097  

Length:

546

Number of predicted TMHs:

0

Exp number of AAs in TMHs:

0.17474

Exp number, first 60 AAs:

0.00028

Total prob of N-in:

0.00779

outside

1  -  546

 

 

Pi

19.095209

Theta

19.58061

Tajima's D

0.027234

CLR

1.284905

CSRT

0.381480925953702

Interpretation

Uncertain